LOCUS       NM_007712               2320 bp    mRNA    linear   ROD 17-JUN-2024
DEFINITION  Mus musculus CDC-like kinase 2 (Clk2), transcript variant 1, mRNA.
ACCESSION   NM_007712
VERSION     NM_007712.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Li,S.Z., Shu,Q.P., Zhou,H.M., Liu,Y.Y., Fan,M.Q., Liang,X.Y.,
            Qi,L.Z., He,Y.N., Liu,X.Y., Du,X.H., Huang,X.C., Chen,Y.Z.,
            Du,R.L., Liang,Y.X. and Zhang,X.D.
  TITLE     CLK2 mediates IkappaBalpha-independent early termination of
            NF-kappaB activation by inducing cytoplasmic redistribution and
            degradation
  JOURNAL   Nat Commun 15 (1), 3901 (2024)
   PUBMED   38724505
  REMARK    GeneRIF: CLK2 mediates IkappaBalpha-independent early termination
            of NF-kappaB activation by inducing cytoplasmic redistribution and
            degradation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2320)
  AUTHORS   Norberto,S., Assalin,H.B., Guadagnini,D., Tobar,N., Boer,P.A.,
            Kang,M.C., Saad,M.J.A., Kim,Y.B. and Prada,P.O.
  TITLE     CLK2 in GABAergic neurons is critical in regulating energy balance
            and anxiety-like behavior in a gender-specific fashion
  JOURNAL   Front Endocrinol (Lausanne) 14, 1172835 (2023)
   PUBMED   37635967
  REMARK    GeneRIF: CLK2 in GABAergic neurons is critical in regulating energy
            balance and anxiety-like behavior in a gender-specific fashion.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2320)
  AUTHORS   Munoz,V.R., Gaspar,R.C., Kuga,G.K., Nakandakari,S.C.B.R.,
            Baptista,I.L., Mekary,R.A., da Silva,A.S.R., de Moura,L.P.,
            Ropelle,E.R., Cintra,D.E. and Pauli,J.R.
  TITLE     Exercise decreases CLK2 in the liver of obese mice and prevents
            hepatic fat accumulation
  JOURNAL   J Cell Biochem 119 (7), 5885-5892 (2018)
   PUBMED   29575149
  REMARK    GeneRIF: High CLK2 expression is associated with obesity.
REFERENCE   5  (bases 1 to 2320)
  AUTHORS   Hatting,M., Rines,A.K., Luo,C., Tabata,M., Sharabi,K., Hall,J.A.,
            Verdeguer,F., Trautwein,C. and Puigserver,P.
  TITLE     Adipose Tissue CLK2 Promotes Energy Expenditure during High-Fat
            Diet Intermittent Fasting
  JOURNAL   Cell Metab 25 (2), 428-437 (2017)
   PUBMED   28089567
  REMARK    GeneRIF: CLK2 is a regulatory component of diet-induced
            thermogenesis in brown adipose tissue.
REFERENCE   6  (bases 1 to 2320)
  AUTHORS   Kunarso,G., Wong,K.Y., Stanton,L.W. and Lipovich,L.
  TITLE     Detailed characterization of the mouse embryonic stem cell
            transcriptome reveals novel genes and intergenic splicing
            associated with pluripotency
  JOURNAL   BMC Genomics 9, 155 (2008)
   PUBMED   18400104
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2320)
  AUTHORS   Pritsker,M., Doniger,T.T., Kramer,L.C., Westcot,S.E. and
            Lemischka,I.R.
  TITLE     Diversification of stem cell molecular repertoire by alternative
            splicing
  JOURNAL   Proc Natl Acad Sci U S A 102 (40), 14290-14295 (2005)
   PUBMED   16183747
REFERENCE   8  (bases 1 to 2320)
  AUTHORS   Ng,P., Wei,C.L., Sung,W.K., Chiu,K.P., Lipovich,L., Ang,C.C.,
            Gupta,S., Shahab,A., Ridwan,A., Wong,C.H., Liu,E.T. and Ruan,Y.
  TITLE     Gene identification signature (GIS) analysis for transcriptome
            characterization and genome annotation
  JOURNAL   Nat Methods 2 (2), 105-111 (2005)
   PUBMED   15782207
REFERENCE   9  (bases 1 to 2320)
  AUTHORS   Nayler,O., Schnorrer,F., Stamm,S. and Ullrich,A.
  TITLE     The cellular localization of the murine serine/arginine-rich
            protein kinase CLK2 is regulated by serine 141 autophosphorylation
  JOURNAL   J Biol Chem 273 (51), 34341-34348 (1998)
   PUBMED   9852100
REFERENCE   10 (bases 1 to 2320)
  AUTHORS   Nayler,O., Stamm,S. and Ullrich,A.
  TITLE     Characterization and comparison of four serine- and arginine-rich
            (SR) protein kinases
  JOURNAL   Biochem J 326 (Pt 3) (Pt 3), 693-700 (1997)
   PUBMED   9307018
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY247622.1, BY021174.1, BY738871.1, DT924403.1, CN538691.1,
            CD740936.1 and AC161600.6.
            
            On Jun 20, 2015 this sequence version replaced NM_007712.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF033564.1, SRR7345562.4601309.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                BY247622.1         1-10
            11-367              BY021174.1         1-357
            368-577             BY738871.1         318-527
            578-711             DT924403.1         203-336             c
            712-910             DT924403.1         1-199               c
            911-1499            CN538691.1         63-651
            1500-2154           CD740936.1         18-672              c
            2155-2320           AC161600.6         170714-170879
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 39.01 cM"
     gene            1..2320
                     /gene="Clk2"
                     /note="CDC-like kinase 2"
                     /db_xref="GeneID:12748"
                     /db_xref="MGI:MGI:1098669"
     exon            1..312
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             313..1812
                     /gene="Clk2"
                     /EC_number="2.7.12.1"
                     /note="isoform 1 is encoded by transcript variant 1; dual
                     specificity protein kinase CLK2"
                     /codon_start=1
                     /product="dual specificity protein kinase CLK2 isoform 1"
                     /protein_id="NP_031738.2"
                     /db_xref="CCDS:CCDS17491.1"
                     /db_xref="GeneID:12748"
                     /db_xref="MGI:MGI:1098669"
                     /translation="MPHPRRYHSSERGSRGSYHEHYQSRKHKRRRSRSWSSSSDRTRR
                     RRREDSYHVRSRSSYDDHSSDRRLYDRRYCGSYRRNDYSRDRGEAYYDTDFRQSYEYH
                     RENSSYRSQRSSRRKHRRRRRRSRTFSRSSSHSSRRAKSVEDDAEGHLIYHVGDWLQE
                     RYEIVSTLGEGTFGRVVQCVDHRRGGTQVALKIIKNVEKYKEAARLEINVLEKINEKD
                     PDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQA
                     VKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDH
                     EHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMM
                     ERILGPVPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEDHHQ
                     LFDLIENMLEYEPAKRLTLGEALQHPFFACLRTEPPNTKLWDSSRDISR"
     exon            313..482
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            483..711
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            712..796
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            797..863
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            864..980
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1147
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1242
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1372
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1455
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1456..1535
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1536..1626
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..2320
                     /gene="Clk2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2131..2136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clk2"
                     /note="hexamer: AATAAA"
     polyA_site      2154
                     /gene="Clk2"
                     /note="major polyA site"
     regulatory      2208..2213
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clk2"
                     /note="hexamer: AATAAT"
     polyA_site      2228
                     /gene="Clk2"
     regulatory      2235..2240
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clk2"
                     /note="hexamer: TATAAA"
     polyA_site      2259
                     /gene="Clk2"
ORIGIN      
        1 gcgtctgtgc gtgcgtgctc gctcacggcg gcggcggcgg cggagcggag aggccagagc
       61 cggagaccga gctgggatcg ggccccgggc gggggcggtg cgagaggcgc cgagcggatc
      121 ctgggggcgg ggacggagcc cgggcgggcg ggactggagc agagcccggg aacggggcgg
      181 gaggtcccag ggccccgggt tggggggtgg aaccgcactt cgtcgccgcg ggggtgccgg
      241 gactccggcc gcagtgccgc cgccatcacg gacttcctgt gggacaagcg cacgggcctc
      301 gccgccagaa cgatgcccca tccccgaagg taccattcct cagagcgagg tagccggggg
      361 agttaccacg aacactatca gagccgaaag cataagcgaa gaagaagtcg ctcctggtca
      421 agtagcagtg accggacaag gcggcggcgg agggaggaca gctaccacgt tcggtcccga
      481 agcagctatg atgaccattc gtccgatcgg cggctgtacg atcggcggta ctgtggcagc
      541 tacaggcgca atgactacag ccgggacaga ggggaggctt actacgacac agactttcgg
      601 cagtcctatg aataccatcg agagaacagc agttaccgaa gccagcgcag cagccgaagg
      661 aaacacagaa ggcggaggag acggagccgg acattcagcc gctcatcttc acacagcagc
      721 cggagagcca agagtgtaga ggacgacgct gagggccacc tcatctacca cgtcggggac
      781 tggctacaag agcgatatga aattgtaagc accttaggag aagggacctt cggccgagtt
      841 gtgcagtgtg tggaccatcg caggggcgga acacaagttg ccctgaagat cattaagaat
      901 gtggagaagt acaaggaagc agcccgacta gaaatcaacg tgctggagaa aatcaatgag
      961 aaagatcctg acaacaagaa cctctgtgtc cagatgtttg actggtttga ctaccatggc
     1021 cacatgtgta tctcctttga gcttctgggc cttagcacct tcgatttcct caaagacaac
     1081 aactacctgc cctaccccat ccaccaagtg cgccacatgg ccttccagct ctgccaggcc
     1141 gtcaagttcc tccatgataa caagttgaca catacggacc tcaaacctga aaatattctg
     1201 tttgtgaatt cagactacga actcacctac aacctagaga agaagcgaga tgagcgcagt
     1261 gtaaagagca cagccgtgcg ggtggtggac ttcggcagtg ccacctttga ccacgaacac
     1321 catagcacca ttgtctccac tcgccattac cgagcccccg aggtcatcct ggagttgggc
     1381 tggtcacagc catgcgatgt atggagcata ggctgcatca tctttgagta ctacgttggc
     1441 ttcaccctct tccagaccca tgacaacaga gagcatctag ccatgatgga aaggatcctg
     1501 ggtcctgtcc cttctcggat gatcagaaag acaagaaaac agaaatattt ttatcggggt
     1561 cgcctggatt gggatgagaa cacctcagcc gggcgctacg ttcgtgagaa ctgcaaacct
     1621 ctgcggcggt atctgacctc agaggcagag gaccaccacc agctcttcga tctgattgaa
     1681 aatatgctag agtatgagcc tgctaagcgg ctgaccttag gtgaagccct tcagcatcct
     1741 ttcttcgcct gccttcggac tgagccaccc aacaccaagt tgtgggactc cagtcgggat
     1801 atcagtcggt gacaattagg ctttgggccc cctgctcctt gtgtagcagt gggtgtccaa
     1861 tccaggacac tggtgctttt ttatacaaaa gaacagaggc agaacttgcc ctctctccag
     1921 cccctggctc ctttgtacct gtgaatatgt ggaatagtgt aaatatgaca gaacttgtac
     1981 ctatcgattc aacccctgac atgtatatac tactatttac actctcccaa cctcatctgc
     2041 ccctcatgtg gaggtggatg gggggcagaa tgacgtaacc aggtggtgtc taccccatgt
     2101 tttataaggg attttgtaca gtctttgtga aataaaatga catgcttcat ctgacccccc
     2161 ccaatccctg gaatttgagg ttcgagggta ctggggtgaa ggagtgaaat aattggtagc
     2221 catctgaatt tgggtataaa ggcctctttg tcctgccaac ttagagtcac ttatatgcac
     2281 tttgtattct gaggtagtaa aaattaaagt agatgcaatc
//
