LOCUS       NM_009904               4066 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus calmegin (Clgn), transcript variant 1, mRNA.
ACCESSION   NM_009904
VERSION     NM_009904.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Weyn-Vanhentenryck,S.M., Feng,H., Ustianenko,D., Duffie,R., Yan,Q.,
            Jacko,M., Martinez,J.C., Goodwin,M., Zhang,X., Hengst,U.,
            Lomvardas,S., Swanson,M.S. and Zhang,C.
  TITLE     Precise temporal regulation of alternative splicing during neural
            development
  JOURNAL   Nat Commun 9 (1), 2189 (2018)
   PUBMED   29875359
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Fujihara,Y., Okabe,M. and Ikawa,M.
  TITLE     GPI-anchored protein complex, LY6K/TEX101, is required for sperm
            migration into the oviduct and male fertility in mice
  JOURNAL   Biol Reprod 90 (3), 60 (2014)
   PUBMED   24501175
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4066)
  AUTHORS   Okabe,M.
  TITLE     Lessons Learned in Andrology: seeing is believing
  JOURNAL   Andrology 2 (1), 3-4 (2014)
   PUBMED   24357627
REFERENCE   4  (bases 1 to 4066)
  AUTHORS   Pandey,R.R., Tokuzawa,Y., Yang,Z., Hayashi,E., Ichisaka,T.,
            Kajita,S., Asano,Y., Kunieda,T., Sachidanandam,R., Chuma,S.,
            Yamanaka,S. and Pillai,R.S.
  TITLE     Tudor domain containing 12 (TDRD12) is essential for secondary PIWI
            interacting RNA biogenesis in mice
  JOURNAL   Proc Natl Acad Sci U S A 110 (41), 16492-16497 (2013)
   PUBMED   24067652
REFERENCE   5  (bases 1 to 4066)
  AUTHORS   Yamaguchi,R., Fujihara,Y., Ikawa,M. and Okabe,M.
  TITLE     Mice expressing aberrant sperm-specific protein PMIS2 produce
            normal-looking but fertilization-incompetent spermatozoa
  JOURNAL   Mol Biol Cell 23 (14), 2671-2679 (2012)
   PUBMED   22621904
REFERENCE   6  (bases 1 to 4066)
  AUTHORS   Tanaka,H., Ikawa,M., Tsuchida,J., Nozaki,M., Suzuki,M.,
            Fujiwara,T., Okabe,M. and Nishimune,Y.
  TITLE     Cloning and characterization of the human Calmegin gene encoding
            putative testis-specific chaperone
  JOURNAL   Gene 204 (1-2), 159-163 (1997)
   PUBMED   9434179
REFERENCE   7  (bases 1 to 4066)
  AUTHORS   Taketo,M.M., Araki,Y., Matsunaga,A., Yokoi,A., Tsuchida,J.,
            Nishina,Y., Nozaki,M., Tanaka,H., Koga,M., Uchida,K., Matsumiya,K.,
            Okuyama,A., Rochelle,J.M., Nishimune,Y., Matsui,M. and Seldin,M.F.
  TITLE     Mapping of eight testis-specific genes to mouse chromosomes
  JOURNAL   Genomics 46 (1), 138-142 (1997)
   PUBMED   9403069
REFERENCE   8  (bases 1 to 4066)
  AUTHORS   Ikawa,M., Wada,I., Kominami,K., Watanabe,D., Toshimori,K.,
            Nishimune,Y. and Okabe,M.
  TITLE     The putative chaperone calmegin is required for sperm fertility
  JOURNAL   Nature 387 (6633), 607-611 (1997)
   PUBMED   9177349
REFERENCE   9  (bases 1 to 4066)
  AUTHORS   Ohsako,S., Hayashi,Y. and Bunick,D.
  TITLE     Molecular cloning and sequencing of calnexin-t. An abundant male
            germ cell-specific calcium-binding protein of the endoplasmic
            reticulum
  JOURNAL   J Biol Chem 269 (19), 14140-14148 (1994)
   PUBMED   8188695
REFERENCE   10 (bases 1 to 4066)
  AUTHORS   Watanabe,D., Yamada,K., Nishina,Y., Tajima,Y., Koshimizu,U.,
            Nagata,A. and Nishimune,Y.
  TITLE     Molecular cloning of a novel Ca(2+)-binding protein (calmegin)
            specifically expressed during male meiotic germ cell development
  JOURNAL   J Biol Chem 269 (10), 7744-7749 (1994)
   PUBMED   8126001
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY100733.1, BC050767.1,
            AC155304.4 and AW456491.1.
            
            On Jul 25, 2016 this sequence version replaced NM_009904.3.
            
            Summary: This gene belongs to the calreticulin family, which
            includes calreticulin, calnexin, and calmegin, and encodes a
            calcium-binding molecular chaperone specifically expressed in
            pachytene stage male germ cells. It is required for the proper
            folding of newly synthesized membrane proteins in the endoplasmic
            reticulum including those critical for sperm migration from the
            uterus into the oviduct and sperm adhesion to and penetration of
            the zona pellucida. This gene plays a key role in spermatogenesis
            and male infertility. Alternative splice variants exist for this
            gene. [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1). Both variants 1 and
            2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050767.1, D14117.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                BY100733.1         1-49
            50-2327             BC050767.1         1-2278
            2328-4049           AC155304.4         49900-51621         c
            4050-4066           AW456491.1         1-17                c
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 39.97 cM"
     gene            1..4066
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /note="calmegin"
                     /db_xref="GeneID:12745"
                     /db_xref="MGI:MGI:107472"
     exon            1..259
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            260..409
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     CDS             269..2104
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; calnexin-T; MEG 1 antigen"
                     /codon_start=1
                     /product="calmegin isoform 1 precursor"
                     /protein_id="NP_034034.2"
                     /db_xref="CCDS:CCDS22452.1"
                     /db_xref="GeneID:12745"
                     /db_xref="MGI:MGI:107472"
                     /translation="MRFQGVGLCLGLLFITVNADFMDDGVEVEDFSENSDESNIKDEP
                     SSGTFKYKTPQPIGEVYFTETFDSGNLAGWVLSKAKKDDMDSEIAIYDGRWEIEELKE
                     NQVPGDRGLVLKSKAKHHAIAAVLEKPFIFADKPLIVQYEVNFQDGIDCGGAYIKLLA
                     DTGDLILENFYDKTSYTIMFGPDKCGEDYKLHLIFRHKHPKTGVFEEKHAKPPDVDLK
                     EFFTDRKTHLYTLVMNPDDTFEVLIDQKVVNQGTLLDDVVPPINPPREIDDPSDKKPE
                     EWDDRAKIPDPTAVRPEDWDENEPAQIEDSSAVKPDGWLDDEPKFIPNPKAEKPEDWS
                     DDMDGEWEAPHIPNPACQIGCGEWKPPMIDNPKYKGIWRPPMINNPNYQGLWSPQKIP
                     NPDYFEDDHPFLLTSFSALGLELWSMTPDIYFDNFIICSEKEVADQWATDGWELKIMV
                     ANANEPGVLRQLVIAAEERPWLWLMYLVMAGLPVALVASFCWPRKVKKKYEDTGPKKT
                     ELCKLQSKAALEQEAEEEKAPEKPEDVQEEKKPGEAEVVTVEKEVIGEPEEKSKEDRE
                     TLEGQEEVSKLSKSGSEDEMKDADESPGSGDAPLKSLRKRRVRKD"
     exon            410..486
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            487..545
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            546..687
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            688..769
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            770..962
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1152
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1266
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1417
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1633
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1759
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1922
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2023
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..4050
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2307..2312
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /note="hexamer: AATAAA"
     polyA_site      2329
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /note="major polyA site"
     regulatory      4028..4033
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /note="hexamer: AATAAA"
     polyA_site      4050
                     /gene="Clgn"
                     /gene_synonym="4930459O04Rik; A2/6; Cln"
                     /note="major polyA site"
ORIGIN      
        1 ggccgctcgc gcacgcgcag ttagcagtcg ctgctgggcg gctgccggcg ggatcgctct
       61 ggggagacgc cgggcaccaa gtagcggcca gtcggacacc tgagctgccg ctgctggaca
      121 caagcccgca ggacaggtgt gtagaccgcg ggccgccttc ctgcggcttg ttctctgggg
      181 accgggtgag aaatcggatc caggtgcccc gcagcatcct cttctgagac tagaagaagg
      241 cttagcttgg tgcggccaga gagtcgatat gcgtttccag ggtgttggac tatgtttggg
      301 tctcctgttc atcacggtca atgcggactt tatggatgat ggtgttgagg tggaagactt
      361 cagtgaaaat tcagacgaga gtaatattaa agatgaacct tcctcgggga cttttaagta
      421 taagacacct caacctatag gagaagtata ttttacagaa acatttgata gtggaaatct
      481 ggctgggtgg gtcttatcaa aagcaaagaa ggatgatatg gattcagaaa ttgctatata
      541 tgatggaaga tgggaaatag aagaactgaa ggaaaaccag gtgcctggcg acagagggct
      601 ggttctgaag tctaaagcca agcaccacgc aatagccgct gtcctcgaaa aaccctttat
      661 ttttgccgat aaacctttga tagttcaata tgaagtaaat tttcaagatg gtattgattg
      721 tggaggtgca tacattaaac tgctagcaga cactggtgat ttgattctgg aaaactttta
      781 tgataaaaca tcctatacca tcatgtttgg accagataaa tgtggagagg attataaact
      841 tcatttaatc ttccgacaca aacatccgaa aactggagtt tttgaagaaa agcatgccaa
      901 gcctccagat gtcgatctga aggagttctt tacagacagg aagactcacc tctataccct
      961 tgtgatgaat ccggatgata catttgaagt attaattgat cagaaagttg tgaaccaagg
     1021 gaccttgcta gatgatgtgg tcccgcctat caacccaccc agagaaattg acgaccccag
     1081 tgataaaaag ccagaggagt gggatgacag agccaagatc cccgacccta ccgccgtcag
     1141 gccagaggac tgggatgaaa atgaacctgc ccaaatagaa gactcaagtg ctgttaaacc
     1201 tgatggctgg cttgatgatg aaccaaagtt tatccccaat cctaaagctg agaaacctga
     1261 ggactggagt gacgacatgg atggagaatg ggaggcacca catattccta atccagcctg
     1321 tcagattgga tgtggagaat ggaaacctcc catgatcgat aacccaaaat acaaaggaat
     1381 ctggagacca ccaatgatca ataatcctaa ctaccaggga ctctggagtc ctcagaagat
     1441 tcccaaccca gattactttg aggatgacca ccccttcctc ctgacttctt tcagtgcgct
     1501 gggcttagag ctttggtcca tgactcctga catctacttt gataatttta tcatctgttc
     1561 agaaaaggag gtggccgatc agtgggctac agatggctgg gaactgaaga taatggtggc
     1621 aaatgctaat gagcctggtg tactccgaca gctggtgata gccgcagagg agcgcccctg
     1681 gctctggctc atgtacctgg tgatggccgg cttgcccgtt gcattagttg cttccttttg
     1741 ttggccaagg aaagtaaaga aaaaatatga agacacggga cctaaaaaaa ctgaattatg
     1801 caaactgcaa agcaaggccg ctctggaaca agaggcagag gaggagaagg ccccagagaa
     1861 gccagaagat gtgcaagaag aaaagaagcc aggtgaagct gaggtcgtca ccgtggaaaa
     1921 ggaagtaatt ggtgaaccgg aagagaagag taaggaagac agagaaactc tagaaggtca
     1981 agaggaagtg agcaagctga gtaaatctgg atcagaggat gagatgaagg acgctgatga
     2041 gagcccagga tctggagatg ccccactgaa gtcattgcgg aagaggaggg tacggaagga
     2101 ttgaagcgtc aagggtcgca aatcccagag agagagagag agagatcggg catctagagt
     2161 cagggtgtcc agaaggacag tgactgcgca tctgcacaaa ctctttctaa gtatctagca
     2221 atgttactct ttcagacact tctttctatc tttcttttgg ggggtggggg gaagtaactt
     2281 tgaagttaac tggtctttga atttagaata aaagaaaagt ggcacattac atgagagcca
     2341 agagattcgt ctccttagaa gttacagctt aacagctgga aggaaggtcg ttttggtttt
     2401 atggagaaaa aataatgtgc tttttgttca cctggtattg gaacacctgc tactgggctc
     2461 tcatctgcag aaaggagcag gttagtttta tcctgagctg taggtttgct ctgttgtaca
     2521 ggctgaaggt gataagagtc cgtgaatgat cgttcaatgc tgtcagcact ataaagagtg
     2581 tgtgagcgag ccacacttcc ctggcttgag ttacactttt cttaagtcag tccacttagc
     2641 ttagagattc cctgaagagg tgctgtgttg gcaggcgtgt caactgagtg gctgtttaag
     2701 agtcactcac tccatcaccg ggaaatatgc aagcatagtt tacaagataa taggcttgtc
     2761 aaaactaaat aacgtacact ttttttttca agctcttgta gccacttcat gcccagtggc
     2821 ttacctcgtt caccaaattg ggtgcacact cactgctctt ggaaatgaaa tttaaatatt
     2881 tttcccatct aaattatatc ctccgtatgg ctcattattg gaaaggtttc ttttattggt
     2941 gtgggattct aacggttgta tccaattctg ccactatgtc tttttaccat gctttcctgc
     3001 tgacaaggtc accctcagag ctgcatctga agtgggagcc caaacaaact accttatcag
     3061 cagtattgct gtatttaaaa tactttattg aagaactttc tactcttaag taccaggtgt
     3121 caaccctttg aaaggccatt caagtccacc tgaagaccag aggatgactt tagtactgag
     3181 tccctatgga acttcttatg agttggggtt tgttttgtaa agatgcacac cccaccttct
     3241 gactccctca cataggtaca ttcagaactc acacactgaa aacaaaccct agcttcacct
     3301 ccttaaaggg ggctgagctg agctaatggc gttgtttgca tcttccatac tggcagaagg
     3361 ggagggagtg cttgccctgt ggccatgcac tgacgttgat agactcctgg tgaacttgta
     3421 acacagctaa ccataactag aatgtactta gcaataatag ggcttaacat agctttcaag
     3481 ggctcatttt tttcctttga attagtcact tgaactaatt ataaataaaa atgtatctca
     3541 aaataaaaaa ctatgcttca gatatttata ccttggtata aatcaggctt gacctgggac
     3601 ttgtgttaaa gctgttttaa ctcttggctg cagattgact ctgcatggac atttgactaa
     3661 actcaggagc ttgcagtggt aatgtctgag caaccgagtt ttatttttat agtttgtcaa
     3721 tacaagggtt tactctgtag ctcaagctga cttggaactc ctagatagcc caggctgcac
     3781 ttagattgat gcaatcctcc tctatcaacc cctcagtact gggacagcag gcgtgaggag
     3841 ccatgcaaac atcactacaa actcctggct gtggatgtgc cgtggctgac aggtcactaa
     3901 actaaaaccc tgtgggggac acatgacatc tatgcaactg aaaagagttt tagtggccaa
     3961 gtggtttttt ttctttattc tagtctgatt ttatttttta taattacttg aatttatcat
     4021 tttccctaat aaaagataaa tatatatgta aaaaaaaaaa aaaaaa
//
