LOCUS       NM_019815               2842 bp    mRNA    linear   ROD 26-JUN-2024
DEFINITION  Mus musculus claudin 18 (Cldn18), transcript variant A1.1, mRNA.
ACCESSION   NM_019815
VERSION     NM_019815.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   De Sanctis,F., Dusi,S., Caligola,S., Anselmi,C., Petrova,V.,
            Rossi,B., Angelini,G., Erdeljan,M., Woll,S., Schlitter,A.M.,
            Metzler,T., Steiger,K., Borok,Z., Bailey,P., Bauer,A., Halin,C.,
            Boschi,F., Giugno,R., Cane,S., Lawlor,R., Corbo,V., Scarpa,A.,
            Constantin,G., Ugel,S., Vascotto,F., Sahin,U., Tureci,O. and
            Bronte,V.
  TITLE     Expression of the membrane tetraspanin claudin 18 on cancer cells
            promotes T lymphocyte infiltration and antitumor immunity in
            pancreatic cancer
  JOURNAL   Immunity 57 (6), 1378-1393 (2024)
   PUBMED   38749447
  REMARK    GeneRIF: Expression of the membrane tetraspanin claudin 18 on
            cancer cells promotes T lymphocyte infiltration and antitumor
            immunity in pancreatic cancer.
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2842)
  AUTHORS   Liu,Y., Sun,Y., Wang,P., Li,S., Dong,Y., Zhou,M., Shi,B., Jiang,H.,
            Sun,R. and Li,Z.
  TITLE     FAP-targeted CAR-T suppresses MDSCs recruitment to improve the
            antitumor efficacy of claudin18.2-targeted CAR-T against pancreatic
            cancer
  JOURNAL   J Transl Med 21 (1), 255 (2023)
   PUBMED   37046312
  REMARK    GeneRIF: FAP-targeted CAR-T suppresses MDSCs recruitment to improve
            the antitumor efficacy of claudin18.2-targeted CAR-T against
            pancreatic cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2842)
  AUTHORS   Fatehullah,A., Terakado,Y., Sagiraju,S., Tan,T.L., Sheng,T.,
            Tan,S.H., Murakami,K., Swathi,Y., Ang,N., Rajarethinam,R., Ming,T.,
            Tan,P., Lee,B. and Barker,N.
  TITLE     A tumour-resident Lgr5+ stem-cell-like pool drives the
            establishment and progression of advanced gastric cancers
  JOURNAL   Nat Cell Biol 23 (12), 1299-1313 (2021)
   PUBMED   34857912
REFERENCE   5  (bases 1 to 2842)
  AUTHORS   Higashi,A.Y., Higashi,T., Furuse,K., Ozeki,K., Furuse,M. and
            Chiba,H.
  TITLE     Claudin-9 constitutes tight junctions of folliculo-stellate cells
            in the anterior pituitary gland
  JOURNAL   Sci Rep 11 (1), 21642 (2021)
   PUBMED   34737342
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2842)
  AUTHORS   Arabzadeh,A., Troy,T.C. and Turksen,K.
  TITLE     Changes in the distribution pattern of Claudin tight junction
            proteins during the progression of mouse skin tumorigenesis
  JOURNAL   BMC Cancer 7, 196 (2007)
   PUBMED   17945025
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2842)
  AUTHORS   Troy,T.C., Li,Y., O'Malley,L. and Turksen,K.
  TITLE     The temporal and spatial expression of Claudins in epidermal
            development and the accelerated program of epidermal
            differentiation in K14-CaSR transgenic mice
  JOURNAL   Gene Expr Patterns 7 (4), 423-430 (2007)
   PUBMED   17182288
REFERENCE   8  (bases 1 to 2842)
  AUTHORS   Hatakeyama,J., Bessho,Y., Katoh,K., Ookawara,S., Fujioka,M.,
            Guillemot,F. and Kageyama,R.
  TITLE     Hes genes regulate size, shape and histogenesis of the nervous
            system by control of the timing of neural stem cell differentiation
  JOURNAL   Development 131 (22), 5539-5550 (2004)
   PUBMED   15496443
REFERENCE   9  (bases 1 to 2842)
  AUTHORS   Kitajiri,S.I., Furuse,M., Morita,K., Saishin-Kiuchi,Y., Kido,H.,
            Ito,J. and Tsukita,S.
  TITLE     Expression patterns of claudins, tight junction adhesion molecules,
            in the inner ear
  JOURNAL   Hear Res 187 (1-2), 25-34 (2004)
   PUBMED   14698084
  REMARK    GeneRIF: Claudin-18 is expressed at the variety of epithelial
            tissues in inner ear including Organ of Corti, stria vascularis,
            Reissner's membrane, spiral limbus, vestibular sensory epithelia,
            and dark cell area.
REFERENCE   10 (bases 1 to 2842)
  AUTHORS   Niimi,T., Nagashima,K., Ward,J.M., Minoo,P., Zimonjic,D.B.,
            Popescu,N.C. and Kimura,S.
  TITLE     claudin-18, a novel downstream target gene for the T/EBP/NKX2.1
            homeodomain transcription factor, encodes lung- and
            stomach-specific isoforms through alternative splicing
  JOURNAL   Mol Cell Biol 21 (21), 7380-7390 (2001)
   PUBMED   11585919
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB663682.1, BX528145.1,
            AF221068.1, AC157949.1 and CF805431.1.
            
            On Aug 13, 2010 this sequence version replaced NM_019815.2.
            
            Summary: This gene encodes a member of the claudin family. Claudins
            are integral membrane proteins and components of tight junction
            strands. Tight junction strands serve as a physical barrier to
            prevent solutes and water from passing freely through the
            paracellular space between epithelial or endothelial cell sheets,
            and also play critical roles in maintaining cell polarity and
            signal transductions. This gene is a downstream target gene
            regulated by the T/EBP/NKX2.1 homeodomain transcription factor.
            Four alternatively spliced transcript variants resulted from
            alternative promoters and alternative splicing have been
            identified, which encode two lung-specific isoforms and two
            stomach-specific isoforms respectively. This gene is also expressed
            in colons, inner ear and skin, and its expression is increased in
            both experimental colitis and ulcerative colitis. [provided by
            RefSeq, Aug 2010].
            
            Transcript Variant: This variant (A1.1) is the lung-specific form.
            It encodes the longer isoform (A1.1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF221068.1, SRR5189673.232647.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849387 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-612               BB663682.1         1-612
            613-648             BX528145.1         532-567
            649-1762            AF221068.1         601-1714
            1763-2495           AC157949.1         95013-95745
            2496-2842           CF805431.1         27-373
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 52.0 cM"
     gene            1..2842
                     /gene="Cldn18"
                     /note="claudin 18"
                     /db_xref="GeneID:56492"
                     /db_xref="MGI:MGI:1929209"
     exon            1..335
                     /gene="Cldn18"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..910
                     /gene="Cldn18"
                     /note="isoform A1.1 precursor is encoded by transcript
                     variant A1.1; Claudin-18"
                     /codon_start=1
                     /product="claudin-18 isoform A1.1 precursor"
                     /protein_id="NP_062789.1"
                     /db_xref="CCDS:CCDS23437.1"
                     /db_xref="GeneID:56492"
                     /db_xref="MGI:MGI:1929209"
                     /translation="MATTTCQVVGLLLSLLGLAGCIAATGMDMWSTQDLYDNPVTAVF
                     QYEGLWRSCVQQSSGFTECRPYFTILGLPAMLQAVRALMIVGIVLGVIGILVSIFALK
                     CIRIGSMDDSAKAKMTLTSGILFIISGICAIIGVSVFANMLVTNFWMSTANMYSGMGG
                     MGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLTPDDSNFKAVSYHASG
                     QNVAYRPGGFKASTGFGSNTRNKKIYDGGARTEDDEQSHPTKYDYV"
     exon            336..500
                     /gene="Cldn18"
                     /inference="alignment:Splign:2.1.0"
     exon            501..627
                     /gene="Cldn18"
                     /inference="alignment:Splign:2.1.0"
     exon            628..738
                     /gene="Cldn18"
                     /inference="alignment:Splign:2.1.0"
     exon            739..2837
                     /gene="Cldn18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2810..2815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cldn18"
                     /note="hexamer: ATTAAA"
     polyA_site      2837
                     /gene="Cldn18"
                     /note="major polyA site"
ORIGIN      
        1 tgagctgagg ggagcttctg attaagagcg ctccccagcg aggcccgagg ccgtgaacct
       61 tcccagcaag agggtggtgg ttgctcctgg aagcctgcgc ccagcagctg aagccatggc
      121 caccaccacg tgccaggtgg tagggcttct cctgtccctc ctgggtctgg ccggctgcat
      181 agccgccact gggatggaca tgtggagcac tcaagacctg tatgacaacc cagtcaccgc
      241 cgtgttccag tatgaagggc tctggaggag ttgcgtgcaa cagagctcgg ggttcaccga
      301 gtgccggcca tacttcacca tcctgggcct tccagccatg ctgcaagctg tacgagccct
      361 gatgatcgtg ggcattgttc tgggggtcat cggtatcctc gtgtccatct tcgccctgaa
      421 gtgcattcgc attggtagca tggatgactc tgccaaggcc aagatgactc tgacttctgg
      481 gatcttgttc atcatctccg gcatctgtgc aatcattggt gtgtctgtgt ttgccaacat
      541 gctggtgacc aacttctgga tgtccacagc taacatgtac agcggcatgg gcggcatggg
      601 tggcatggtg cagaccgttc agaccaggta cacctttggt gcagctctgt tcgtgggctg
      661 ggttgctgga ggcctcaccc tgattggggg agtgatgatg tgcatcgcct gccgtggcct
      721 gacaccagat gacagcaact tcaaagctgt gtcttaccat gcctctggcc aaaatgttgc
      781 ctacaggcct ggaggcttta aggccagcac tggctttggg tccaacacca gaaacaagaa
      841 gatctacgat gggggtgccc gcacagaaga cgatgaacag tctcatccta ccaagtatga
      901 ctatgtgtag tgctctaaga cccgccaacc tgtgtgcagg aggaaccctt ccccaagaag
      961 agctcacccc aaagcaacgg gagtctacct tgttcccttg ttgatttcaa ctgacatctg
     1021 aaagttggta aagcctgatt ttcatccata gggaggctag acagtcttgg ccacatgtgt
     1081 ctgcctctaa atatcccatc acaaaacagc tgagttatcg tttatgagtt agaggccata
     1141 acactcactt tagcccaacc ctctgctttt taccgtagac tttcttttca tctggtgatg
     1201 gaatggaatt tgactcacag actaatactt taatggttta gagaaacttt ccttcctcgt
     1261 acttaataag cctgctgatg gtcgattttc cagcttgacc accaagggaa attttaaaag
     1321 gaaaaaaaaa tacattaaaa ggcattattt cctactcaat tgtgccttac ccacccccaa
     1381 cttgactgat aataataatg aacaccactt aaagaaagaa tgccagagga aagatagttg
     1441 tgtttccccc cagccagtca tctgagtccc cctatgtggt gatctagaac attactcgcc
     1501 acagtgattt tcaaagaagg caagcgagcc tgttcgctct gctcagcatc tgctgattcc
     1561 agcaaggccc ttccagagct ttccactaga agtcctcctt ctctcggaag tcagaaattc
     1621 cccctagaag agtaagaaat agattctttt gggtaacctg agtcctaggt atagttataa
     1681 taaatagtat attagcaaaa cggtttggta tctcagtgaa ttagtttcag ccttacatat
     1741 agaaaaagct ggggaaaaaa aagcatccct tgacattgtc tatagcgtaa gatcctatat
     1801 aaatccaagc ttcaacaaaa gctcactgag tctaatagtt ttcttttgag gtctccacgg
     1861 ccttagtact catagatgca gcccctgttt aaaagtaaaa aaattaaagt agcttaaaac
     1921 gggttctttt tttttttttt tttttcaaaa aatccaatag agacctgtgt gtctggcata
     1981 gctacagtta ctgccaatcg acagggccac ttctttggtc ctgtaggcag ttttgcagtt
     2041 ctgacagctg cgccgggcat caatatgcag accacaccct tctctgtgct tgtaggacga
     2101 cccgttcaag gagaaagcat gaactccatc tccatgtgag cctgaatgct cccaggaaat
     2161 ggagataggg tgctctctaa aacccacctg aacctgaaac agctgtagcg ctatgctgta
     2221 agagcctggc catcaagttc ctatggagaa aaagggcagt ccttgcatta atagtgcata
     2281 tataagtggc ctctgggggg cagggatgaa tattcagtgg tggctccgag tatgtacaga
     2341 ccgtctaagg agctgtgttg accaagagcc aggttaatac gcagagtttt tcccactggg
     2401 actacagtga ttttagacta tactgaagaa ggccctctgg aaaatcatta tctgaaatgg
     2461 cataaagaat gaacagacca aacaatttaa ggggaggggg caggtggaag gagggggaag
     2521 gaggtagaaa taagcaatct agggcatgaa gattgttaag gttcttgggg tccaaatgga
     2581 aggtcacccc tttgaggcca tggacacaat gcaccccacc cctaccccca cctgcccacc
     2641 caccagaaag tccctggtcg gactggaggc agtgagaatc agctgttttc agttagtggg
     2701 tctcggtgta gcacctggct gtttcaaagc ttccccttgc tttgccgttt tttccgccat
     2761 tgctgtcttg ttttctgtgt tattaacctc catgttttgt acgttaaata ttaaaacact
     2821 gttaacatcc attcaaaaaa aa
//
