LOCUS       NM_001193659            3804 bp    mRNA    linear   ROD 28-SEP-2023
DEFINITION  Mus musculus claudin 12 (Cldn12), transcript variant 2, mRNA.
ACCESSION   NM_001193659
VERSION     NM_001193659.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Chen JT, Hu X, Otto IUC, Schurger C, von Bieberstein BR, Doppler K,
            Krug SM, Hankir MK, Blasig R, Sommer C, Brack A, Blasig IE and
            Rittner HL.
  TITLE     Myelin barrier breakdown, mechanical hypersensitivity, and
            painfulness in polyneuropathy with claudin-12 deficiency
  JOURNAL   Neurobiol Dis 185, 106246 (2023)
   PUBMED   37527762
  REMARK    GeneRIF: Myelin barrier breakdown, mechanical hypersensitivity, and
            painfulness in polyneuropathy with claudin-12 deficiency.
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Xing W, Pourteymoor S, Chen Y and Mohan S.
  TITLE     Targeted Deletion of the Claudin12 Gene in Mice Increases Articular
            Cartilage and Inhibits Chondrocyte Differentiation
  JOURNAL   Front Endocrinol (Lausanne) 13, 931318 (2022)
   PUBMED   35937800
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3804)
  AUTHORS   Cao H, Ni C, Han L, Wang R, Blasig R, Haseloff R, Qin Y, Lan J, Lou
            X, Ma P, Yao X, Wang L, Wang F, Zhu L, Lei N, Blasig IE and Qin Z.
  TITLE     Claudin-12 Deficiency Inhibits Tumor Growth by Impairing
            Transendothelial Migration of Myeloid-Derived Suppressor Cells
  JOURNAL   Cancer Res 82 (13), 2472-2484 (2022)
   PUBMED   35580275
REFERENCE   4  (bases 1 to 3804)
  AUTHORS   Beggs MR, Young K, Pan W, O'Neill DD, Saurette M, Plain A, Rievaj
            J, Doschak MR, Cordat E, Dimke H and Alexander RT.
  TITLE     Claudin-2 and claudin-12 form independent, complementary pores
            required to maintain calcium homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 118 (48) (2021)
   PUBMED   34810264
  REMARK    GeneRIF: Claudin-2 and claudin-12 form independent, complementary
            pores required to maintain calcium homeostasis.
REFERENCE   5  (bases 1 to 3804)
  AUTHORS   Higashi AY, Higashi T, Furuse K, Ozeki K, Furuse M and Chiba H.
  TITLE     Claudin-9 constitutes tight junctions of folliculo-stellate cells
            in the anterior pituitary gland
  JOURNAL   Sci Rep 11 (1), 21642 (2021)
   PUBMED   34737342
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3804)
  AUTHORS   Hashizume A, Ueno T, Furuse M, Tsukita S, Nakanishi Y and Hieda Y.
  TITLE     Expression patterns of claudin family of tight junction membrane
            proteins in developing mouse submandibular gland
  JOURNAL   Dev Dyn 231 (2), 425-431 (2004)
   PUBMED   15366020
REFERENCE   7  (bases 1 to 3804)
  AUTHORS   Takasato M, Osafune K, Matsumoto Y, Kataoka Y, Yoshida N, Meguro H,
            Aburatani H, Asashima M and Nishinakamura R.
  TITLE     Identification of kidney mesenchymal genes by a combination of
            microarray analysis and Sall1-GFP knockin mice
  JOURNAL   Mech Dev 121 (6), 547-557 (2004)
   PUBMED   15172686
REFERENCE   8  (bases 1 to 3804)
  AUTHORS   Kitajiri SI, Furuse M, Morita K, Saishin-Kiuchi Y, Kido H, Ito J
            and Tsukita S.
  TITLE     Expression patterns of claudins, tight junction adhesion molecules,
            in the inner ear
  JOURNAL   Hear Res 187 (1-2), 25-34 (2004)
   PUBMED   14698084
  REMARK    GeneRIF: Claudin-12 is expressed at the variety of epithelial
            tissues in inner ear including Organ of Corti, stria vascularis,
            Reissner's membrane, spiral limbus, vestibular sensory epithelia,
            and dark cell area.
REFERENCE   9  (bases 1 to 3804)
  AUTHORS   Nitta T, Hata M, Gotoh S, Seo Y, Sasaki H, Hashimoto N, Furuse M
            and Tsukita S.
  TITLE     Size-selective loosening of the blood-brain barrier in
            claudin-5-deficient mice
  JOURNAL   J Cell Biol 161 (3), 653-660 (2003)
   PUBMED   12743111
REFERENCE   10 (bases 1 to 3804)
  AUTHORS   Niimi T, Nagashima K, Ward JM, Minoo P, Zimonjic DB, Popescu NC and
            Kimura S.
  TITLE     claudin-18, a novel downstream target gene for the T/EBP/NKX2.1
            homeodomain transcription factor, encodes lung- and
            stomach-specific isoforms through alternative splicing
  JOURNAL   Mol Cell Biol 21 (21), 7380-7390 (2001)
   PUBMED   11585919
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB845860.1, AK039672.1,
            AK128999.1 and AC068663.5.
            
            [WARNING] On Nov 22, 2023 this sequence was replaced by
            NM_001193659.2.
            
            Summary: This gene encodes a member of the claudin family. Claudins
            are integral membrane proteins and components of tight junction
            strands. Tight junction strands serve as a physical barrier to
            prevent solutes and water from passing freely through the
            paracellular space between epithelial or endothelial cell sheets,
            and also play critical roles in maintaining cell polarity and
            signal transductions. This gene, along with several other family
            members, is expressed in the inner ear. The protein encoded by this
            gene and another family member, claudin 2, are critical for vitamin
            D-dependent Ca2+ absorption between enterocytes. Multiple
            alternatively spliced transcript variants encoding the same protein
            have been found. [provided by RefSeq, Oct 2011].
            
            Transcript Variant: This variant (2) differs in the 5' UTR, as
            compared to variant 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK039672.1, SRR7652917.842987.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-119               BB845860.1         2-120
            120-824             AK039672.1         2-706
            825-3708            AK128999.1         694-3577
            3709-3804           AC068663.5         43295-43390
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 2.98 cM"
     gene            1..3804
                     /gene="Cldn12"
                     /note="claudin 12"
                     /db_xref="GeneID:64945"
                     /db_xref="MGI:MGI:1929288"
     exon            1..222
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     exon            223..312
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     exon            313..364
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     exon            365..3804
                     /gene="Cldn12"
                     /inference="alignment:Splign:2.1.0"
     CDS             394..1128
                     /gene="Cldn12"
                     /codon_start=1
                     /product="claudin-12"
                     /protein_id="NP_001180588.1"
                     /db_xref="CCDS:CCDS19074.1"
                     /db_xref="GeneID:64945"
                     /db_xref="MGI:MGI:1929288"
                     /translation="MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRN
                     EKNLTIYTGLWVKCARYDGSSDCLMYDRTWYLSVDQLDLRVLQFALPLSIVIAMGALL
                     LCLIGMCNTAFNSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWAIFYNSH
                     LNRKFEPVFTFDYAVFVTIASSGGLFMTALLLFVWYCACKSLSSPFWQPLYSHAPGMH
                     TYSQPYSSRSRLSAIEIDIPVVSHST"
ORIGIN      
        1 agaacgcact tccgcggcag cggagggaga ccaaggagcc gcggctcgga gtgggtgagc
       61 gggatgcccg agccgtgagg agccggtagg tgtggtcagg gacgctcccg tttccgagtc
      121 ttttgcaagt agttgaaggt gttgcgtgaa acggcgaagc ggcggcgagt cggcatttga
      181 aaggttgccc acatgggtgg acgtggaaaa gtggccgagg aggtccagat tatctctgct
      241 ttgtgtgcag atgtgctcct gttgcatctg agttcactaa gctgactttg gaggatcagg
      301 cacagggaaa ggggatcaga ggcgcagaca ggctgcttgg agaaacgctg attaccttga
      361 aattactgac catacagctc aagccctcct gccatgggct gccgagatgt ccacgcagcc
      421 accgtcctgt ccttcctgtg tggtattgcc tctgtcgcag gcctctttgc ggggactctg
      481 cttcctaact ggaggaaact gcggctgatc acattcaaca gaaacgagaa gaacctgacg
      541 atttacacgg gcctgtgggt gaagtgtgcc cggtatgatg gaagcagtga ctgcctgatg
      601 tacgaccgta cgtggtacct gtcggttgac cagctggacc tgcgtgtcct ccagtttgcc
      661 ctgcctctca gcatcgtgat cgcaatgggt gccttgctac tctgcctgat tggaatgtgt
      721 aacacggcct tcaattcttc cgtgcctaac atcaaactgg ccaagtgtct ggtcaatagt
      781 gcaggctgcc acctggtggc cggactcctg ttttttctgg caggtaccgt gagcctctct
      841 ccgtccatct gggccatctt ttataacagc catctcaaca ggaagtttga gccggtcttt
      901 acctttgact atgcagtatt tgtcactatt gctagctcag ggggtctgtt tatgactgct
      961 ctcctgctgt tcgtttggta ttgtgcatgc aagtctttgt cctctccttt ctggcaaccg
     1021 ctgtactctc acgctcccgg gatgcacact tactcacagc cctattcatc acggtcccgc
     1081 ctctctgcca ttgaaatcga cattccagta gtctcacaca gcacttaaca gagatacaat
     1141 taactgttca aaggatgtct tgtagcctca cagccccctg cataatgagc ctttttggac
     1201 ctgtgtacat ttttcctatg ttccttatca gtcagtggag ccaaatttgt atatcttggt
     1261 agaatgaaat gctgctagat tttatgaggt acatattaca ttcttctaaa tgtgaaccat
     1321 tccttttatc ttgcttctga gactagaagg gtctctagcc tccttgttga tgtctgatca
     1381 atagcttatg tgaaaggacc catgataaca ctggaggcag tgaagacagc atattgaaca
     1441 atgctgtttg atcttcaccc gtatttcaga ttgtgcattg actccgatga ttgaaaggtg
     1501 gaaggggcag tttcaaactt aagacgtttc agtcggatga ggctaggagt ttgtctgtct
     1561 ccagaactct tccagcttga aggtataatt tcattctctc tggggactaa ttgaataggc
     1621 atcagtatac tgaagattgc tatcttctac ctcattccag ggaaaagtaa aacgcatttt
     1681 acatgacaga gtaaagctaa tatttaagca tggttaacat ttggcttctt cattggtttc
     1741 agatggggtc ttgctgtgta gctctggctg gcctagagat cactgcagac cagtgtgtac
     1801 tcagactttc tacccgcttg cctcctgagc actaactaca aggcacaccc aattacacta
     1861 acaatgcgtc aggagacagt cagtatgttg cttcatttga agcaagaatc cctttccaga
     1921 tccccttatc aggtagtgct catgcgctgg ccaagctctt agtgcctagg acaaaattgt
     1981 ttttcagggc gcgggagtct gttctttagt gtcagcgcca aagattcatt tggtggctac
     2041 tatttgagta gtggctttag tctgttcagt tcctgcttca gaactgattt cttgcttctg
     2101 acactgctta acgtttgagt aagtacttca gaggatacac aaataggaga aatagccatg
     2161 tctatgtagg aatgttgatc taattcacct gataaccagt gggaagtctg acattatggt
     2221 agctggtaag cttgtcaagc attgacccag gagtgtattt catctgaaag atgtctttgt
     2281 gacacagaca gcctgtggtc caccaccagc aatagcctgg acgctggaaa ttccctttaa
     2341 tcaggtctcc tcccatagcc gtaataatgg tctcagatca tgagaatggt aagataggag
     2401 agagattaaa ttatacctat tctacctctg aagctaagat gataaattaa agaggggcaa
     2461 attttaaaaa tatctcttct gatgtgatat caaatggata aaaccatgtt agtactaaca
     2521 tgacattgag gggtgttggg tcttagctta gggatttgag ctgctcattt agataatgtc
     2581 cacgtactag cattgtttgt aaggactgta gtacattgtg cttatagatt tttacaagca
     2641 gctgatttta tgataacagt ttctgatgcc tcttttgcaa atctgagtct ttctgtagag
     2701 atatgtagta cttttcccta agtccaacac tactgtatgt aggccaacac tactgtatgt
     2761 aggcaagtta atccaattgt ataattttaa aacgccgtca atacaaagca tgcacatcta
     2821 tggtttttta aaattacaac ttctagcctt ttccacctct ggttcttctc actcataaat
     2881 ggagctgagt gttggtcatt ccaagacagt ggcagctcga attctgagac taataatagt
     2941 gttttccctt tgttaattaa tcttagttct tctctcacta aactgtttcc agatggaatt
     3001 ctaccatgcc ttggtgttag tatacagaga taaaagtagg gccaggtgta gtggcttttg
     3061 actttaatac cagcactcag gaggcagtga caggtgcatt tctggaaatt ctggtctcca
     3121 tagcaagttc caggccagtc agagctacac agtgagaacc tgtctttaaa aacaattaga
     3181 aaaagtggac tcagcaatga gaatactaga cagttttcag ctcacaggat tgtaactaca
     3241 aacatttaag ctttcttatg agaatctttc ggcattatca tctatagcct caaattactg
     3301 ttatgaattc actcaggaag agaaacagct gtttctgtat tatcttttta gtgttccaga
     3361 ttgggcataa atagctgttt tttaaagcca tcaaaggaaa gcacgtattt acaatgcttt
     3421 tgagtcactg gggctgttat tgagatgggg tggataactt cttttgttgc taaaaagctg
     3481 ttgattttaa aggccttcta aggagccagt taggcaaaga accttgaatg tgagacggtg
     3541 catagaattt ctgttaccca gctttgggag aagaataaac ctgagattct gagttcactc
     3601 tgcttgtaca tacgccctat gacggatagt gtgtgctgta aaccacaagg tagaatcctc
     3661 agtgggtgtg ctgtgctgcc tcatgtttat taaaagatgt atttttacaa gtttgtctct
     3721 gtccttttct cagctctaag gttaaatatt ttggaaaaca tttgtcatgt gtatgtcaac
     3781 gaaaataaag cactcctaaa gacc
//
