LOCUS       NM_019701               2344 bp    mRNA    linear   ROD 26-JUN-2024
DEFINITION  Mus musculus chloride channel, voltage-sensitive Kb (Clcnkb), mRNA.
ACCESSION   NM_019701
VERSION     NM_019701.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Pyrshev,K., Khayyat,N.H., Stavniichuk,A., Tomilin,V.N., Zaika,O.,
            Ramkumar,N. and Pochynyuk,O.
  TITLE     ClC-K2 Cl- channel allows identification of A- and B-type of
            intercalated cells in split-opened collecting ducts
  JOURNAL   FASEB J 36 (5), e22275 (2022)
   PUBMED   35349181
REFERENCE   3  (bases 1 to 2344)
  AUTHORS   Lin,M.H., Chen,J.C., Tian,X., Lee,C.M., Yu,I.S., Lo,Y.F.,
            Uchida,S., Huang,C.L., Chen,B.C. and Cheng,C.J.
  TITLE     Impairment in renal medulla development underlies salt wasting in
            Clc-k2 channel deficiency
  JOURNAL   JCI Insight 6 (20), e151039 (2021)
   PUBMED   34499620
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2344)
  AUTHORS   Khayyat,N.H., Zaika,O., Tomilin,V.N., Pyrshev,K. and Pochynyuk,O.
  TITLE     Angiotensin II increases activity of the ClC-K2 Cl- channel in
            collecting duct intercalated cells by stimulating production of
            reactive oxygen species
  JOURNAL   J Biol Chem 296, 100347 (2021)
   PUBMED   33524393
  REMARK    GeneRIF: Angiotensin II increases activity of the ClC-K2 Cl(-)
            channel in collecting duct intercalated cells by stimulating
            production of reactive oxygen species.
REFERENCE   5  (bases 1 to 2344)
  AUTHORS   Honda,K., Kim,S.H., Kelly,M.C., Burns,J.C., Constance,L., Li,X.,
            Zhou,F., Hoa,M., Kelley,M.W., Wangemann,P., Morell,R.J. and
            Griffith,A.J.
  TITLE     Molecular architecture underlying fluid absorption by the
            developing inner ear
  JOURNAL   Elife 6, e26851 (2017)
   PUBMED   28994389
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2344)
  AUTHORS   Kobayashi,K., Uchida,S., Mizutani,S., Sasaki,S. and Marumo,F.
  TITLE     Intrarenal and cellular localization of CLC-K2 protein in the mouse
            kidney
  JOURNAL   J Am Soc Nephrol 12 (7), 1327-1334 (2001)
   PUBMED   11423561
REFERENCE   7  (bases 1 to 2344)
  AUTHORS   Winters,C.J., Zimniak,L., Mikhailova,M.V., Reeves,W.B. and
            Andreoli,T.E.
  TITLE     Cl(-) channels in basolateral TAL membranes XV. Molecular
            heterogeneity between cortical and medullary channels
  JOURNAL   J Membr Biol 177 (3), 221-230 (2000)
   PUBMED   11014860
REFERENCE   8  (bases 1 to 2344)
  AUTHORS   Uchida,S., Tanaka,Y., Ito,H., Saitoh-Ohara,F., Inazawa,J.,
            Yokoyama,K.K., Sasaki,S. and Marumo,F.
  TITLE     Transcriptional regulation of the CLC-K1 promoter by myc-associated
            zinc finger protein and kidney-enriched Kruppel-like factor, a
            novel zinc finger repressor
  JOURNAL   Mol Cell Biol 20 (19), 7319-7331 (2000)
   PUBMED   10982849
REFERENCE   9  (bases 1 to 2344)
  AUTHORS   Matsumura,Y., Uchida,S., Kondo,Y., Miyazaki,H., Ko,S.B., Hayama,A.,
            Morimoto,T., Liu,W., Arisawa,M., Sasaki,S. and Marumo,F.
  TITLE     Overt nephrogenic diabetes insipidus in mice lacking the CLC-K1
            chloride channel
  JOURNAL   Nat Genet 21 (1), 95-98 (1999)
   PUBMED   9916798
REFERENCE   10 (bases 1 to 2344)
  AUTHORS   Kere,J.
  TITLE     Kidney kinetics and chloride ion pumps
  JOURNAL   Nat Genet 21 (1), 67-68 (1999)
   PUBMED   9916791
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB845352.1, AK146876.1, AA240610.1 and BX636867.1.
            
            On Apr 4, 2008 this sequence version replaced NM_019701.1.
            
            Summary: This gene is a member of the CLC family of voltage-gated
            chloride channels. The gene is located adjacent to a highly similar
            chloride channel gene on chromosome 4. This gene is syntenic with
            human CLCNKA (geneID:1187). [provided by RefSeq, Sep 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849385
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                BB845352.1         2-13
            13-624              AK146876.1         2-613
            625-1598            AK146876.1         688-1661
            1599-2109           AA240610.1         1-511
            2110-2339           AK146876.1         2172-2401
            2340-2344           BX636867.1         1-5                 c
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 74.02 cM"
     gene            1..2344
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /note="chloride channel, voltage-sensitive Kb"
                     /db_xref="GeneID:56365"
                     /db_xref="MGI:MGI:1930643"
     exon            1..41
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            42..148
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..2112
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /note="putative basolateral mTAL chloride channel ClC-Ka;
                     clC-K2; chloride channel K1-like; putative basolateral
                     cTAL chloride channel ClC-Ka; chloride channel Kb"
                     /codon_start=1
                     /product="chloride channel protein ClC-Kb"
                     /protein_id="NP_062675.2"
                     /db_xref="CCDS:CCDS18872.1"
                     /db_xref="GeneID:56365"
                     /db_xref="MGI:MGI:1930643"
                     /translation="MEELVGLREGASKKPVPLQELWGPCPRIRRNIQGGLEWLKERLF
                     RVGEDWYFLVALGVLMALISYAMNFTIGRVVRAHKWLYREIGDGHLLRYLSWTVYPVA
                     LLSFSSGFSQSITPSSGGSGIPEVKTILTGVVLEDYLDIKNFGAKVVGLSCTLATGST
                     IFLGKLGPFVHLSVMIAAYLGRVRTKTVGEPESKTKEMELLAAGAAVGVATVFAAPIS
                     GVLFSIEVMSSHFSVWDYWRGFFAATCGAFMFHLLAVFNNEQETITSIYKTSFPVDIP
                     FDLPEIFFFVALGAICGILSCGYNYSQRTFLFFLKANGFTSKLLATSKPLYSALAAVV
                     LASITYPPGVGHFMASRLSMSEHLETLFDNNSWALMTKNSSPPWAAEPDPQKLWLEWC
                     HPQLTVFGTLVFFLVMKFWMLILATTIPIPAGYFLPIFIYGAVIGRLFGEVLSVAFPE
                     GIVAGGRVNPIMPGAYALAGAAAFSGAVTHTLSTALLAFEVTGQLVHALPVLMAVLAA
                     NAISQSFQPSFYDGTIIVKKLPYLPWIRGRQIGSHSVTVGHFMNCALTTLAKDMPLEQ
                     VIQVVISTDVTQYPLVETTESQTLVGVVKRTHLVQALQTEPASWAPGQQPCLQDILAN
                     GCPTQPVTLQLSLETSLHETHNLFELLNLQTLFVTSRGRAVGSVSWVELKKAISTLTN
                     PPAPK"
     exon            149..277
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            278..406
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            407..546
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            547..624
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            625..703
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            704..829
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            830..914
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1016
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1101
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1275
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1345
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1456
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1670
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1804
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1893
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..1977
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..2064
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     exon            2065..2340
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2322..2327
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /note="hexamer: AATAAA"
     polyA_site      2340
                     /gene="Clcnkb"
                     /gene_synonym="Clc-Ka; Clck2; CLCKB; Clcnk1l; Clcnk2"
                     /note="major polyA site"
ORIGIN      
        1 ggaggagggc ctgttggggc ttcagcaggg cacactgaca gagaaaccat ggaggaatta
       61 gtggggcttc gtgagggcgc ctccaagaag ccagtgccac tgcaagaact ctgggggcca
      121 tgcccacgca tccgcagaaa catccagggg ggcctagagt ggctgaaaga gcggctcttc
      181 cgtgtgggtg aggactggta cttcctggtg gctctcgggg tgctcatggc tctgatcagc
      241 tatgctatga acttcactat tgggcgcgtg gtcagagcac acaagtggct gtacagggag
      301 attggagatg gccacctgct ccggtacctc tcctggaccg tgtaccccgt ggctcttctg
      361 tccttctcct ccggcttctc acagagcatc acaccatcct ctggagggtc tggaatccct
      421 gaggtgaaga ccatcttgac cggagtggtc ctggaggact acctagacat caagaacttc
      481 ggggccaagg tggtgggcct ctcctgcacc ctggccacag gcagtaccat cttcctggga
      541 aaattgggtc ccttcgtgca cctgagcgtg atgatcgctg cttacctggg ccgcgtgcgc
      601 accaagaccg tcggggaacc tgagagcaag accaaagaaa tggaactgtt ggccgcagga
      661 gcagcagtgg gtgtggccac ggtcttcgca gctcccatca gcggtgtcct gttcagcatc
      721 gaggtcatgt cctctcactt ctccgtctgg gattactgga ggggcttctt cgctgccacc
      781 tgtggggcct ttatgttcca cctcctggca gtcttcaaca atgaacagga gaccatcacc
      841 tccatctaca agaccagctt tccagtggac ataccctttg acttaccaga gatcttcttt
      901 tttgtggccc tgggggccat ctgtggcatc ctgagctgcg ggtacaacta cagccagcgg
      961 actttcttat ttttcctcaa ggccaatggg ttcacctcca aactgctggc taccagcaaa
     1021 cccttgtact cagccctggc tgctgtggtc ctggcttcca tcacgtatcc ccctggtgtg
     1081 ggccacttca tggcttcccg gctgtccatg tcggagcatt tggagacact gtttgacaac
     1141 aactcctggg cactgatgac taagaactcg tctccaccct gggcagcgga gcctgatccc
     1201 cagaagctgt ggttggagtg gtgtcaccca cagttgactg tctttgggac actagtcttc
     1261 ttcctggtca tgaagttctg gatgctgatt ttggcaacca ccatccccat ccctgctgga
     1321 tacttcttgc ccatctttat ctatggagct gtcattgggc gcctctttgg ggaggttcta
     1381 tctgtcgcct tcccagaggg cattgtggct ggaggcaggg tcaatcccat catgcccggg
     1441 gcctatgctc tggcaggtgc tgctgccttc tcaggggcgg tgacccacac cctctccacg
     1501 gcactgctgg cctttgaggt aacgggccag ctcgttcacg cactgcctgt gctgatggct
     1561 gtgctggcgg ccaacgccat ctctcagagc ttccagccct ctttctacga tggcaccatc
     1621 attgtcaaga aactgccgta cctgccgtgg atccgtggcc gacagattgg ttcccactct
     1681 gtaactgtgg gtcacttcat gaactgcgcc ctcactacac tggccaagga catgcccctg
     1741 gagcaagtga tccaagtcgt gatctccaca gatgtgaccc agtaccccct ggtggagact
     1801 acagagtctc agaccctggt gggcgttgta aaaaggaccc acttggtgca agccctccag
     1861 actgagccag cttcctgggc tccaggccag cagccctgtc tccaggatat cttggctaat
     1921 ggctgcccca cccagccagt gacactgcag ctgtccctgg agacctcctt gcatgagaca
     1981 cacaaccttt ttgagctgct gaaccttcag acgctgttcg tgacgtcacg aggcagagct
     2041 gtgggctctg tatcgtgggt ggagctgaag aaagcaattt caaccctgac caatcctcct
     2101 gcccccaagt gagtcagcct ggggggggtc ggcagcctgg cacccagctg ctcagctgcc
     2161 atggatctgg gatctctcag cccggcaaga catctccact cagcagagaa gaacgctcca
     2221 tattcacact ccgatccctg aaggagctga gagtaaatga agacattgtg acccttgtga
     2281 cttctggggt tgcctgctgc taaccaggag cttctgagaa aaataaaact gatgtctgga
     2341 aaaa
//
