LOCUS       NM_024412               2409 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus chloride channel, voltage-sensitive Ka (Clcnka),
            transcript variant 1, mRNA.
ACCESSION   NM_024412 XM_982619
VERSION     NM_024412.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Lin,M.H., Chen,J.C., Tian,X., Lee,C.M., Yu,I.S., Lo,Y.F.,
            Uchida,S., Huang,C.L., Chen,B.C. and Cheng,C.J.
  TITLE     Impairment in renal medulla development underlies salt wasting in
            Clc-k2 channel deficiency
  JOURNAL   JCI Insight 6 (20), e151039 (2021)
   PUBMED   34499620
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2409)
  AUTHORS   Wang,H., Morris,R.G., Knepper,M.A. and Zhou,X.
  TITLE     Sickle cell disease up-regulates vasopressin, aquaporin 2, urea
            transporter A1, Na-K-Cl cotransporter 2, and epithelial Na channels
            in the mouse kidney medulla despite compromising urinary
            concentration ability
  JOURNAL   Physiol Rep 7 (8), e14066 (2019)
   PUBMED   31033226
REFERENCE   4  (bases 1 to 2409)
  AUTHORS   Nomura,N., Shoda,W., Wang,Y., Mandai,S., Furusho,T., Takahashi,D.,
            Zeniya,M., Sohara,E., Rai,T. and Uchida,S.
  TITLE     Role of ClC-K and barttin in low potassium-induced sodium chloride
            cotransporter activation and hypertension in mouse kidney
  JOURNAL   Biosci Rep 38 (1) (2018)
   PUBMED   29326302
  REMARK    GeneRIF: our study provides in vivo evidence that, in response to a
            low-potassium diet, ClC-K and barttin play important roles in the
            activation of the WNK4-SPAK-NCC cascade and blood pressure
            regulation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2409)
  AUTHORS   Hennings,J.C., Andrini,O., Picard,N., Paulais,M., Huebner,A.K.,
            Cayuqueo,I.K., Bignon,Y., Keck,M., Corniere,N., Bohm,D.,
            Jentsch,T.J., Chambrey,R., Teulon,J., Hubner,C.A. and Eladari,D.
  TITLE     The ClC-K2 Chloride Channel Is Critical for Salt Handling in the
            Distal Nephron
  JOURNAL   J Am Soc Nephrol 28 (1), 209-217 (2017)
   PUBMED   27335120
REFERENCE   6  (bases 1 to 2409)
  AUTHORS   Akizuki,N., Uchida,S., Sasaki,S. and Marumo,F.
  TITLE     Impaired solute accumulation in inner medulla of Clcnk1-/- mice
            kidney
  JOURNAL   Am J Physiol Renal Physiol 280 (1), F79-F87 (2001)
   PUBMED   11133517
REFERENCE   7  (bases 1 to 2409)
  AUTHORS   Winters,C.J., Zimniak,L., Mikhailova,M.V., Reeves,W.B. and
            Andreoli,T.E.
  TITLE     Cl(-) channels in basolateral TAL membranes XV. Molecular
            heterogeneity between cortical and medullary channels
  JOURNAL   J Membr Biol 177 (3), 221-230 (2000)
   PUBMED   11014860
REFERENCE   8  (bases 1 to 2409)
  AUTHORS   Uchida,S., Tanaka,Y., Ito,H., Saitoh-Ohara,F., Inazawa,J.,
            Yokoyama,K.K., Sasaki,S. and Marumo,F.
  TITLE     Transcriptional regulation of the CLC-K1 promoter by myc-associated
            zinc finger protein and kidney-enriched Kruppel-like factor, a
            novel zinc finger repressor
  JOURNAL   Mol Cell Biol 20 (19), 7319-7331 (2000)
   PUBMED   10982849
REFERENCE   9  (bases 1 to 2409)
  AUTHORS   Matsumura,Y., Uchida,S., Kondo,Y., Miyazaki,H., Ko,S.B., Hayama,A.,
            Morimoto,T., Liu,W., Arisawa,M., Sasaki,S. and Marumo,F.
  TITLE     Overt nephrogenic diabetes insipidus in mice lacking the CLC-K1
            chloride channel
  JOURNAL   Nat Genet 21 (1), 95-98 (1999)
   PUBMED   9916798
REFERENCE   10 (bases 1 to 2409)
  AUTHORS   Kere,J.
  TITLE     Kidney kinetics and chloride ion pumps
  JOURNAL   Nat Genet 21 (1), 67-68 (1999)
   PUBMED   9916791
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ068439.1, AY373832.1, BB743618.1 and BG061970.1.
            
            On Apr 7, 2009 this sequence version replaced NM_024412.2.
            
            Summary: This gene is a member of the CLC family of voltage-gated
            chloride channels. The gene is located adjacent to a highly similar
            chloride channel gene on chromosome 4. This gene is syntenic with
            human CLCNKB (geneID:1188). Multiple alternatively spliced
            variants, encoding the same protein, have been found for this gene.
            [provided by RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC037077.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-55                CJ068439.1         5-59
            56-2130             AY373832.1         7-2081
            2131-2383           BB743618.1         161-413
            2384-2409           BG061970.1         1-26                c
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 73.94 cM"
     gene            1..2409
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /note="chloride channel, voltage-sensitive Ka"
                     /db_xref="GeneID:12733"
                     /db_xref="MGI:MGI:1329026"
     exon            1..50
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            51..155
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..2119
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /note="chloride channel K1; putative basolateral cTAL
                     chloride channel ClC-Ka; chloride channel Ka"
                     /codon_start=1
                     /product="chloride channel protein ClC-Ka"
                     /protein_id="NP_077723.3"
                     /db_xref="CCDS:CCDS18871.1"
                     /db_xref="GeneID:12733"
                     /db_xref="MGI:MGI:1329026"
                     /translation="MEELVGLREGSSGKPVTLQELWGPCPRIRRGIRGGLEWLKERLF
                     RAREDWYFLVALGVLMALISYAMNFAIGRVVRAHKWLYREVGDGHLLRYLSWTVYPVA
                     LLSFSSGFSQSITPFSGGSGLPELKTMLSGVVLENYLDIKNFGAKVVGLSCTLATGST
                     IFLGKVGPFVHLSVMISAYLGRVRAKAVGDTESKAKEVEMLSAAAAVGVATVFAAPFS
                     GVLFSIEVMSSHFSVWNYWRGFFAATCGAFMFRLLAVFNSEQETITSIYKTRFRVDVP
                     FDLPEIFFFVALGFICGILSCVYLFCQRNFLRFIKTNRYTSKLLATSKPSYAALVALV
                     LASVTYPPGVGRFMASRLSMAEHLHSLFDNNSWALMTRNASPPWPSEPDPQNLWFEWY
                     HPRFTIFGTLAFFLIMKFWMLILATTIPMPAGYFMPIFIIGAAIGRLLGEALSVAFPE
                     GIVAGGEVYPIMPGGYALAGAAAFSGAVTHTISTALLAFELTGQIVHALPVLMAVLAA
                     NVISQNLQPSFYDGTIMAKKLPYLPWIRGRQIGSYPVTVEHFMNSNLTTLAKDMPLEE
                     VVKVVTSTDVSQYPLVETRESQTLVGIVERTHLVQALQTQPASWAPGQERFLQDILAG
                     GCPTQPVTLQLSPETSLYQAHRLFELLTLQALFVTSRGRAVGSVSWVELKKAISTLIN
                     PPAPK"
     exon            156..284
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            285..413
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            414..553
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            554..631
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            632..710
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            711..836
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            837..921
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            922..1023
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1024..1108
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1282
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1352
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1353..1463
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1677
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1678..1811
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1900
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..1984
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2071
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
     exon            2072..2409
                     /gene="Clcnka"
                     /gene_synonym="CLC-K1; Clcnk1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgttagaga agcaccagcc aggcaggacc tctacagggt ggaccgggag gacggatgga
       61 agagctggtg gggctgcgtg agggctcctc cgggaagccg gtgactctgc aagagctatg
      121 gggtccgtgt ccacgcatcc gccgaggcat cagagggggc ctagagtggc tgaaagagcg
      181 gctgttccgt gcgcgtgagg actggtactt cctggtggct cttggggtgc tcatggctct
      241 gatcagctat gccatgaact ttgctatcgg gcgtgtggtc agagcacaca agtggctgta
      301 cagagaagtt ggggacggcc acctgctccg gtacctctcc tggaccgtgt accccgtagc
      361 tcttctgtcc ttctcctccg gcttctcaca gagcattacg cccttctctg gagggtctgg
      421 actcccagag ctgaagacca tgctgtctgg agtagtcctg gagaactacc tagacatcaa
      481 gaacttcggg gccaaggtgg tgggcctctc ctgcaccctg gccacaggca gcaccatctt
      541 cctaggcaaa gtgggcccgt tcgtgcacct gagtgtgatg atttctgctt atctgggccg
      601 cgtgcgcgcc aaagccgtcg gggatactga gagcaaggcc aaggaagttg aaatgctgtc
      661 ggcggcagcg gcggtgggcg tggccacagt cttcgccgcc cccttcagtg gtgtcctgtt
      721 cagcatcgag gtcatgtcct ctcacttctc cgtctggaat tactggaggg gcttcttcgc
      781 tgccacctgt ggggccttca tgttccgtct cctggcggtc ttcaacagtg aacaggagac
      841 catcacctcc atctacaaga ccagattccg agtggatgtg ccctttgacc tgcctgaaat
      901 cttcttcttt gtggctctgg ggttcatctg cgggatcctc agttgcgttt acctgttctg
      961 tcagcgaaat tttctccgct tcatcaagac caatcgctac acctccaaac tgctggctac
     1021 aagcaagcct tcctacgcag ctctggtcgc gctggtcctg gcttcagtca cctatcctcc
     1081 tggtgtgggc cgtttcatgg cttcccggct gtccatggcg gaacacttgc actctctgtt
     1141 tgacaacaac tcgtgggcgt tgatgactcg gaatgcgtcc ccaccctggc cttctgagcc
     1201 cgacccccag aacctgtggt ttgaatggta ccacccacgg ttcaccatct ttgggacgct
     1261 ggccttcttc ctgatcatga agttctggat gctgattctg gccaccacga tccccatgcc
     1321 tgcaggctac ttcatgccta tattcatcat cggagctgcc atcgggcgcc tcttgggaga
     1381 ggccctgtct gtcgccttcc cagagggcat tgtggctggc ggagaggtct atcccatcat
     1441 gcctgggggc tatgctctgg caggtgctgc tgccttctcc ggagcagtga cccacaccat
     1501 ctccacagcg ctgctggcct ttgagctgac tggccagatc gttcacgcac tgcctgtgct
     1561 gatggctgtg ctggcggcca atgtcatctc ccaaaactta cagccctctt tctacgatgg
     1621 caccatcatg gccaagaaac tgccgtacct gccgtggatc cgtggccgac agattggctc
     1681 ctaccctgta acggtggaac atttcatgaa ctctaacctc accacactgg ccaaggacat
     1741 gcccctggag gaggtggtca aggtggtgac ctccacagat gtgtctcagt atcccttggt
     1801 ggagaccaga gagtctcaga ccctggtggg cattgtggaa aggacccact tggtgcaagc
     1861 ccttcagacc cagccagctt cctgggctcc aggccaagag cgatttctcc aggacatctt
     1921 ggctggtggc tgccccacac agccagtgac cctgcagctg tccccagaga cctccctata
     1981 tcaggcacac aggctctttg agctgctgac ccttcaggcg ctgttcgtga cgtcacgggg
     2041 cagagctgtg ggctctgtgt cctgggtgga gctgaagaaa gccatttcca ccttgatcaa
     2101 cccaccagcc cccaagtgag ccgtgtgggg gtcggaacag gaacccagct gagggctcca
     2161 gatgctgcac agagccctgc cccttttccc tccctccctg cactcctcca gcccagccca
     2221 gctactcagt gcatcagcca gctctaccca gctgaacagg gctccacatt tatcacggcc
     2281 ccccgagagg acaacatctc tctggtctgg aagggacaaa tctcaccccg gtttacgaga
     2341 cgtaccttct gggattatct attcacagcc agtagctcct gagaaaataa aactaaagct
     2401 gattcccag
//
