LOCUS       NM_001416768            3144 bp    mRNA    linear   ROD 07-AUG-2024
DEFINITION  Mus musculus chloride channel, voltage-sensitive 2 (Clcn2),
            transcript variant 2, mRNA.
ACCESSION   NM_001416768 XM_017316862
VERSION     NM_001416768.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Fu,S.J., Hu,M.C., Hsiao,C.T., Cheng,A.T., Chen,T.Y., Jeng,C.J. and
            Tang,C.Y.
  TITLE     Regulation of ClC-2 Chloride Channel Proteostasis by Molecular
            Chaperones: Correction of Leukodystrophy-Associated Defect
  JOURNAL   Int J Mol Sci 22 (11), 5859 (2021)
   PUBMED   34070744
  REMARK    GeneRIF: Regulation of ClC-2 Chloride Channel Proteostasis by
            Molecular Chaperones: Correction of Leukodystrophy-Associated
            Defect.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Goppner,C., Soria,A.H., Hoegg-Beiler,M.B. and Jentsch,T.J.
  TITLE     Cellular basis of ClC-2 Cl- channel-related brain and testis
            pathologies
  JOURNAL   J Biol Chem 296, 100074 (2021)
   PUBMED   33187987
REFERENCE   3  (bases 1 to 3144)
  AUTHORS   Catalan,M.A., Julio-Kalajzic,F., Niemeyer,M.I., Cid,L.P. and
            Sepulveda,F.V.
  TITLE     Short Chain Fatty Acids Effect on Chloride Channel ClC-2 as a
            Possible Mechanism for Lubiprostone Intestinal Action
  JOURNAL   Cells 9 (8), 1781 (2020)
   PUBMED   32722648
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3144)
  AUTHORS   Fu,S.J., Hu,M.C., Peng,Y.J., Fang,H.Y., Hsiao,C.T., Chen,T.Y.,
            Jeng,C.J. and Tang,C.Y.
  TITLE     CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2
            Chloride Channels: Implication for Aldosteronism and Leukodystrophy
  JOURNAL   Cells 9 (6), 1332 (2020)
   PUBMED   32466489
  REMARK    GeneRIF: CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis
            of ClC-2 Chloride Channels: Implication for Aldosteronism and
            Leukodystrophy.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3144)
  AUTHORS   Collin,G.B., Gogna,N., Chang,B., Damkham,N., Pinkney,J., Hyde,L.F.,
            Stone,L., Naggert,J.K., Nishina,P.M. and Krebs,M.P.
  TITLE     Mouse Models of Inherited Retinal Degeneration with Photoreceptor
            Cell Loss
  JOURNAL   Cells 9 (4), 931 (2020)
   PUBMED   32290105
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3144)
  AUTHORS   Speake,T., Kajita,H., Smith,C.P. and Brown,P.D.
  TITLE     Inward-rectifying anion channels are expressed in the epithelial
            cells of choroid plexus isolated from ClC-2 'knock-out' mice
  JOURNAL   J Physiol 539 (Pt 2), 385-390 (2002)
   PUBMED   11882672
REFERENCE   7  (bases 1 to 3144)
  AUTHORS   Hubner,C.A., Stein,V., Hermans-Borgmeyer,I., Meyer,T., Ballanyi,K.
            and Jentsch,T.J.
  TITLE     Disruption of KCC2 reveals an essential role of K-Cl cotransport
            already in early synaptic inhibition
  JOURNAL   Neuron 30 (2), 515-524 (2001)
   PUBMED   11395011
REFERENCE   8  (bases 1 to 3144)
  AUTHORS   Bosl,M.R., Stein,V., Hubner,C., Zdebik,A.A., Jordt,S.E.,
            Mukhopadhyay,A.K., Davidoff,M.S., Holstein,A.F. and Jentsch,T.J.
  TITLE     Male germ cells and photoreceptors, both dependent on close
            cell-cell interactions, degenerate upon ClC-2 Cl(-) channel
            disruption
  JOURNAL   EMBO J 20 (6), 1289-1299 (2001)
   PUBMED   11250895
REFERENCE   9  (bases 1 to 3144)
  AUTHORS   Joo,N.S., Clarke,L.L., Han,B.H., Forte,L.R. and Kim,H.D.
  TITLE     Cloning of ClC-2 chloride channel from murine duodenum and its
            presence in CFTR knockout mice
  JOURNAL   Biochim Biophys Acta 1446 (3), 431-437 (1999)
   PUBMED   10524221
REFERENCE   10 (bases 1 to 3144)
  AUTHORS   Lengeling,A., Gronemeier,M., Ronsiek,M., Thiemann,A., Jentsch,T.J.
            and Jockusch,H.
  TITLE     Chloride channel 2 gene (Clc2) maps to chromosome 16 of the mouse,
            extending a region of conserved synteny with human chromosome 3q
  JOURNAL   Genet Res 66 (2), 175-178 (1995)
   PUBMED   8522159
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT010490.10 and AC087898.19.
            
            On Feb 16, 2023 this sequence version replaced XM_017316862.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7974084.20783.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-206               CT010490.10        61149-61354         c
            207-363             CT010490.10        58853-59009         c
            364-516             CT010490.10        58547-58699         c
            517-645             CT010490.10        58120-58248         c
            646-779             CT010490.10        57894-58027         c
            780-857             CT010490.10        57712-57789         c
            858-936             CT010490.10        57552-57630         c
            937-1062            CT010490.10        57297-57422         c
            1063-1147           CT010490.10        57132-57216         c
            1148-1249           CT010490.10        56930-57031         c
            1250-1334           CT010490.10        55795-55879         c
            1335-1490           CT010490.10        55512-55667         c
            1491-1560           CT010490.10        55120-55189         c
            1561-1671           CT010490.10        54810-54920         c
            1672-1885           CT010490.10        54342-54555         c
            1886-2019           CT010490.10        53874-54007         c
            2020-2192           CT010490.10        53534-53706         c
            2193-2307           CT010490.10        53317-53431         c
            2308-2381           CT010490.10        53083-53156         c
            2382-2441           CT010490.10        52813-52872         c
            2442-2480           CT010490.10        52659-52697         c
            2481-2585           CT010490.10        52414-52518         c
            2586-2672           AC087898.19        1473-1559
            2673-3144           AC087898.19        1677-2148
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 12.5 cM"
     gene            1..3144
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /note="chloride channel, voltage-sensitive 2"
                     /db_xref="GeneID:12724"
                     /db_xref="MGI:MGI:105061"
     exon            1..206
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     CDS             120..2867
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /note="isoform 2 is encoded by transcript variant 2;
                     chloride channel protein 2; chloride channel 2"
                     /codon_start=1
                     /product="chloride channel protein 2 isoform 2"
                     /protein_id="NP_001403697.1"
                     /db_xref="GeneID:12724"
                     /db_xref="MGI:MGI:105061"
                     /translation="MAAATAAAAAAAAAGEGMEPRALQYEQTLMYGRYTQELGAFAKE
                     EAARIRLGGPEPWKGSPSARATPELLEYGQSRCARCRICSVRCHKFLVSRVGEDWIFL
                     VLLGLLMALVSWAMDYAIAVCLQGEGKGWRAQQWMSRGLNTNILLQYLAWVTYPVVLI
                     TFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFVAKVIGLTCALGSGMPLG
                     KEGPFVHIASMCAALLSKFLSLFGGIYEHESRNTEMLAAACAVGVGCCFAAPIGGVLF
                     SIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQ
                     ELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTL
                     TFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEDLELPSTSQAWSPPRANVFLTL
                     VIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYR
                     IVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPS
                     LYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKG
                     RMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQKLRKAQLSPPSDQESPP
                     SSETSIRFQVNTEDSGFSGAHGQTHKPLKPALKRGPSNSTSLQEGTTGNMESAGIALR
                     SLFCGSPPLEATSELEKSESCDKRKLKRVRISLASDSDPEAEMSPEEILEWEEQQLDE
                     PVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAI
                     EGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPRSRHGLPREGTPSDSDDKC
                     Q"
     exon            207..363
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            364..516
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            517..645
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            646..779
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            780..857
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            858..936
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1062
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1147
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1249
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            1250..1334
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1490
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1560
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1671
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1885
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2019
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2192
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2307
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2381
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2441
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            2442..2480
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            2481..2585
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            2586..2672
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     exon            2673..3144
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3095..3100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /note="hexamer: AATAAA"
     regulatory      3112..3117
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /note="hexamer: AATAAA"
     polyA_site      3117
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /note="major polyA site"
     polyA_site      3144
                     /gene="Clcn2"
                     /gene_synonym="ClC-2; Clc2; nmf240"
                     /note="major polyA site"
ORIGIN      
        1 gcactctgca ctcggccgcc tgggctgagg ggacgggacg ggtgcgggcg cgggctcagc
       61 gggccgggag ctgagtcaag gccgagaagc agagggaggc aagaggacag tgcaccgaga
      121 tggcggctgc aacggctgcg gcggccgcgg cggcggcggc gggggaaggg atggagcctc
      181 gagcgctgca gtacgagcag accctgatgt atggccggta cactcaggaa ctcggggcct
      241 ttgccaaaga ggaagctgct cgtattcgcc tgggagggcc tgagccctgg aaggggtccc
      301 cttctgcccg ggctacccca gagctcctag aatatggaca gagccgatgt gccagatgtc
      361 gcatttgttc tgtacgctgc cacaagttcc tggtgtccag ggtcggtgaa gactggatct
      421 tcctggttct gttggggctc ctcatggcac tggtcagctg ggctatggac tatgccatcg
      481 ctgtctgtct acagggtgag ggcaaaggct ggcgagctca gcaatggatg tcccggggct
      541 taaacaccaa catcttactc cagtacctgg cttgggttac ctaccccgtg gtcctcatca
      601 ctttctctgc tggattcacc cagatcctgg ccccacaggc tgtcgggtct ggcatccccg
      661 aaatgaaaac catccttcgg ggagtggtgc tgaaagaata cctcaccctc aagacctttg
      721 tagctaaggt cattgggcta acctgtgccc tgggcagtgg gatgcccctt ggcaaagagg
      781 gaccctttgt gcacattgcc agcatgtgtg ccgcccttct cagcaagttc ctctccctct
      841 ttgggggtat ctatgagcat gagtcccgga acacggagat gctagctgct gcatgcgcag
      901 taggagtggg ctgctgcttt gccgcaccaa tcggaggggt cctattcagc attgaagtca
      961 cctccacctt cttcgctgtt aggaactact ggcggggctt ctttgcggcc accttcagtg
     1021 ccttcatctt tcgggtcttg gcagtgtgga accgtgatga agaaaccatc acagctctct
     1081 tcaaaactcg gttccgactc gacttcccat ttgacctgca agagctgcca gcctttgctg
     1141 tcattggcat tgctagtggc ttcgggggag ccctctttgt ctacctgaac cggaagattg
     1201 tccaggtgat gcggaagcaa aaaaccatca accgcttcct catgaggaaa cggctgctct
     1261 tcccggcact ggtgactctg ctcatctcca ctctgacctt cccccctggc tttggacagt
     1321 tcatggccgg acagctctca cagaaggaga ccctagtcac tctgtttgac aaccggacgt
     1381 gggtccgcca gggcctggtt gaggatctag agctacccag cacttcacag gcctggagcc
     1441 caccacgtgc caatgtcttc cttactctgg tcatcttcat cctcatgaag ttctggatgt
     1501 ctgcactggc taccactatc ccagtgccct gtggggcctt catgcctgtc tttgtcattg
     1561 gagcggcatt tgggcggctg gtgggcgaaa gcatggccgc ctggttccca gatgggattc
     1621 acacagatag cagcacctac cgaattgtac ctggaggcta tgctgtggtc ggggcggctg
     1681 cactcgcagg agcagtgaca cacacagtgt ccacagcagt gattgtcttc gagctcacgg
     1741 gccagatcgc tcacattctg cctgtcatga ttgctgtcat cctggctaat gctgttgccc
     1801 agagcctgca gccatcgctc tatgacagta tcatccgcat caagaagctg ccctacctac
     1861 ctgagctggg ctggggccgc caccagcagt accgggtgcg agtcgaggac atcatggttc
     1921 gggatgtacc ccatgtagcc ctcagctgca cttttcggga cctgcggttg gcactgcaca
     1981 gaaccaaggg ccgtatgttg gccctagtgg agtctcctga gtccatgatc ctactgggat
     2041 ccatcgaacg ctcacaggtg gtagcactac taggagccca gctgagccca gcgcgcaggc
     2101 ggcagcacat gcaaaagcta agaaaagccc agctgtctcc accgtcggat caggagagcc
     2161 cccctagctc cgagacatct atccgcttcc aggtgaacac agaggactcg ggcttctctg
     2221 gagcccacgg gcagactcac aagcccctga agcctgctct aaagagaggg cccagcaaca
     2281 gtacaagcct gcaggaaggt accacaggca acatggagtc agcaggcatt gccctcagaa
     2341 gcctcttctg tggcagtcca cctctggagg caacatcaga attggaaaag tcagaatcct
     2401 gtgacaagcg caagctgaag cgggtccgaa tctccctggc gagtgactca gacccggaag
     2461 ccgagatgag tcctgaggag atcttagagt gggaagaaca gcagctagat gagccagtca
     2521 acttcagtga ctgcaaaatc gaccctgccc ccttccagct ggtggagcgg acttctttgc
     2581 acaagaccca caccatcttc tcattgctgg gagtggacca tgcttatgtc accagcattg
     2641 gcagactcat tgggattgtc accctaaagg agctccggaa ggccattgaa ggctctgtca
     2701 cagcacaggg tgttaaagtc aggccacccc tcgccagttt ccgggacagt gccaccagca
     2761 gcagtgacac agagaccact gaggtgcatg cgctctgggg gccaagatcc cgccacggcc
     2821 tcccacgaga gggtaccccc tccgacagtg atgacaagtg ccagtgaacc ccttacggat
     2881 ggcctgtatt ccttctgcaa gcagcagtga ccagaaactg gaacccaagg ccaccctaac
     2941 tgctggggga tcatcaagtg tcctggcggg atgaggtggg gttctatggc ccctgcccca
     3001 tctttgagaa aagggcagaa ctaaactggg tttatactgg agcctccaat gacaagatgt
     3061 atatagaaat ttacaaagat ttttatatta atttaataaa acaataaata gaataaacac
     3121 ctaattagcc actcatgtat agaa
//
