LOCUS       NM_207208               2998 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Mus musculus chloride channel accessory 4A (Clca4a), mRNA.
ACCESSION   NM_207208 XM_131262
VERSION     NM_207208.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Rajagopal,M., Kathpalia,P.P., Widdicombe,J.H. and Pao,A.C.
  TITLE     Differential effects of extracellular ATP on chloride transport in
            cortical collecting duct cells
  JOURNAL   Am J Physiol Renal Physiol 303 (4), F483-F491 (2012)
   PUBMED   22647633
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Bothe,M.K., Mundhenk,L., Beck,C.L., Kaup,M. and Gruber,A.D.
  TITLE     Impaired autoproteolytic cleavage of mCLCA6, a murine integral
            membrane protein expressed in enterocytes, leads to cleavage at the
            plasma membrane instead of the endoplasmic reticulum
  JOURNAL   Mol Cells 33 (3), 251-257 (2012)
   PUBMED   22350745
  REMARK    GeneRIF: cleavage of the mCLCA6 E157Q variant protein is
            zinc-dependent and sensitive to metalloprotease inhibitors,
            suggesting secondary cleavage by a metalloprotease.
REFERENCE   3  (bases 1 to 2998)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   4  (bases 1 to 2998)
  AUTHORS   Piirsoo,M., Meijer,D. and Timmusk,T.
  TITLE     Expression analysis of the CLCA gene family in mouse and human with
            emphasis on the nervous system
  JOURNAL   BMC Dev Biol 9, 10 (2009)
   PUBMED   19210762
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2998)
  AUTHORS   Bothe,M.K., Braun,J., Mundhenk,L. and Gruber,A.D.
  TITLE     Murine mCLCA6 is an integral apical membrane protein of non-goblet
            cell enterocytes and co-localizes with the cystic fibrosis
            transmembrane conductance regulator
  JOURNAL   J Histochem Cytochem 56 (5), 495-509 (2008)
   PUBMED   18285349
  REMARK    GeneRIF: mCLCA6 is colocalized with CFTR at the surface of colonic
            crypt cells
REFERENCE   6  (bases 1 to 2998)
  AUTHORS   Patel,A.C., Morton,J.D., Kim,E.Y., Alevy,Y., Swanson,S., Tucker,J.,
            Huang,G., Agapov,E., Phillips,T.E., Fuentes,M.E., Iglesias,A.,
            Aud,D., Allard,J.D., Dabbagh,K., Peltz,G. and Holtzman,M.J.
  TITLE     Genetic segregation of airway disease traits despite redundancy of
            calcium-activated chloride channel family members
  JOURNAL   Physiol Genomics 25 (3), 502-513 (2006)
   PUBMED   16569774
REFERENCE   7  (bases 1 to 2998)
  AUTHORS   Evans,S.R., Thoreson,W.B. and Beck,C.L.
  TITLE     Molecular and functional analyses of two new calcium-activated
            chloride channel family members from mouse eye and intestine
  JOURNAL   J Biol Chem 279 (40), 41792-41800 (2004)
   PUBMED   15284223
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK033591.1.
            
            On Jul 19, 2006 this sequence version replaced NM_207208.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK033591.1, AY560902.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 69.22 cM"
     gene            1..2998
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /note="chloride channel accessory 4A"
                     /db_xref="GeneID:99663"
                     /db_xref="MGI:MGI:2139744"
     exon            1..196
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     CDS             32..2809
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /note="calcium-activated chloride channel regulator 4;
                     chloride channel calcium activated 6; chloride channel
                     calcium activated 4A; calcium-activated chloride channel
                     regulator 4A; mClca6; calcium-activated chloride channel
                     regulator 6; calcium activated chloride channel"
                     /codon_start=1
                     /product="calcium-activated chloride channel regulator 4A
                     precursor"
                     /protein_id="NP_997091.3"
                     /db_xref="CCDS:CCDS38661.1"
                     /db_xref="GeneID:99663"
                     /db_xref="MGI:MGI:2139744"
                     /translation="MMAFSRGPVFLLLLLYLLWGSDTSLIRLNENGYEDIIIAIDPAV
                     PEDTTIIEHIKGMVTKASTYLFEATEKRFFFKNVSILIPESWKDSPQYRRPKQESYKH
                     ADIKVAPPTVEGRDEPYTRQFTQCEEKAEYIHFTPDFVLGRKQDEYGDSGKVLVHEWA
                     HLRWGVFDEYNEDQPFYSASSKKIEATRCSTGITGTNRVYACQGGSCAMRRCRTNSTT
                     KLYEKDCQFFPDKVQSEKASIMFMQSIDSVTEFCKKENHNREAPTLHNKKCNYRSTWE
                     VISTSEDFNSSTPMETSPSPPFFSLLRISERIMCLVLDVSGSMTSYDRLNRMNQAAKY
                     FLSQIIENRSWVGMVHFSSQATIVHELIQINSDIERNQLLQTLPTSANGGTSICSGIK
                     AAFQVFKNGEYQTDGTEILLLSDGEDSTAKDCIDEVKDSGSIVHFIALGPSADLAVTN
                     MSILTGGNHKLATDEAQNNGLIDAFGALASENADITQKSLQLESKGAILNNSLWLNDT
                     VVIDSTLGRDTFFLVTWSKQAPAIYLRDPKGTQTTNFTMDSASKMAYLSIPGTAQVGV
                     WTYNLEAKENSEILTITVTSRAANSSVPPITVNAKVNTDTNTFPSPMIVYAEVLQGYT
                     PIIGARVTATIESNSGKTEELVLLDNGAGADAFKDDGVYSRFFTAYSVNGRYSLKVRA
                     DGGRNSARRSLRHPSSRAAYIPGWVVDGEIQGNPPRPETTEATQPVLEDFSRTASGGA
                     FVMSNVPIGPLPDVYPPNRITDLQATLDGEEISLTWTAPGDDYDVGRVQQYIIRTSKN
                     IIELRDNFNNSPRVDTTNLTPKEANSEETFAFKPENITEENATYIFIAIESVDKSSLS
                     SGPSNIAQVALFTPQAEPDPDESPSLSGVSVATIVLSVLGALVLVCIIVGTTICILKN
                     KRSSSAAITKF"
     exon            197..337
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            338..485
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            486..594
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            595..772
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            773..991
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1219
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1397
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1504
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1720
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            1721..1988
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2159
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2393
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2998
                     /gene="Clca4a"
                     /gene_synonym="9130020L07Rik; Clca4; Clca6"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gaaacaaagg cctctgggtg accaggctgg aatgatggct ttctccagag ggcctgtttt
       61 cctcctcctc ctcctgtacc tgctttgggg gtcagacact tccttgataa ggctgaatga
      121 gaatggttat gaagacatca ttattgccat tgatcctgct gtgccagaag atacaacaat
      181 aattgaacac ataaagggaa tggtgactaa agcatctaca tacctttttg aagctacaga
      241 aaaaagattt ttcttcaaaa atgtatctat attaattcct gagagttgga aagacagtcc
      301 ccagtacagg aggccaaaac aggagagtta caagcatgct gacattaaag tggcacctcc
      361 caccgttgag ggcagagatg agccctacac caggcaattc acacagtgtg aagagaaagc
      421 agaatacatc cacttcaccc ctgactttgt actggggaga aaacaagatg aatatggaga
      481 ttcaggaaaa gtgttggtcc atgaatgggc ccaccttcgc tggggagtat ttgatgaata
      541 caatgaagac cagccattct acagcgcttc atcaaagaaa atcgaagcaa caaggtgctc
      601 cacaggtatc acggggacga atagagtcta tgcatgccaa gggggcagct gtgcgatgag
      661 aaggtgcaga actaattcca ccacaaagtt atatgaaaag gattgtcaat tcttccctga
      721 taaagttcaa tcagaaaagg cttccataat gttcatgcaa agtatcgatt ctgtgactga
      781 attctgtaaa aaggaaaacc ataatcgaga ggctccaact ctacataata aaaagtgcaa
      841 ttacagaagc acttgggaag taatcagcac ttctgaggat tttaacagca gcacgcccat
      901 ggagacgtca ccctctccac ccttcttctc cctgctgagg atcagtgaga gaattatgtg
      961 cctagttctt gatgtgtctg ggagtatgac ttcttatgat cgcctgaatc gaatgaacca
     1021 agcagcaaaa tactttctaa gccagataat tgaaaatagg tcctgggtag ggatggtgca
     1081 ctttagtagc caggccacta ttgtgcatga gctgatccaa ataaacagtg acatcgagag
     1141 aaaccagctc ttgcaaacct tacctacaag tgccaatgga gggacgtcca tctgctctgg
     1201 aatcaaggct gcatttcagg tatttaaaaa tggagaatac caaacagatg ggactgagat
     1261 cttgctgttg agtgatggtg aggacagcac agccaaagac tgtattgatg aggtgaaaga
     1321 cagcgggtcc atagttcatt tcattgcctt gggaccatcg gctgacttag ctgtaacaaa
     1381 catgagcatt ttaacaggtg gaaaccataa gcttgctaca gatgaagccc agaacaatgg
     1441 actcattgat gctttcgggg ccctggcttc agaaaatgct gatatcaccc agaagtcact
     1501 tcagcttgaa agtaagggtg ccatactgaa caatagcctc tggctgaatg acactgttgt
     1561 aattgacagc accttgggaa gggacacatt ctttcttgtc acctggagca aacaagcccc
     1621 tgccatttat ctcagggatc ccaaaggaac acaaaccacc aatttcacaa tggactctgc
     1681 ttccaaaatg gcctatctca gtattccagg aacagctcag gtgggtgttt ggacttacaa
     1741 cctggaagcc aaagaaaact cagagatact aacgattaca gtaacctctc gggcagcaaa
     1801 ttcttctgtg ccaccaatca ctgtaaatgc taaggtaaac acggacacta acactttccc
     1861 cagcccaatg attgtgtatg ctgaagttct acaaggttac actcccatca ttggagcccg
     1921 agtgacagcc accatagaat ctaacagtgg gaagacagaa gagctagtcc ttctggacaa
     1981 cggtgcaggg gctgatgctt tcaaggatga tggggtctac tccaggttct tcacagctta
     2041 ttcagtgaat ggcaggtata gcttaaaagt ccgtgcagat ggaggaagaa attctgcgag
     2101 gaggagttta cgccacccat caagcagagc tgcatacata ccaggctggg tagtagatgg
     2161 agaaattcaa gggaacccac ccagacccga aacgactgag gctactcagc cagtcttgga
     2221 ggatttcagc aggacagcat ctggaggtgc ctttgtgatg tccaatgttc caattggtcc
     2281 attgcctgat gtatacccac caaatcgaat cacagacctc caggccacac tcgatgggga
     2341 agagatcagt ctaacatgga cggccccagg agatgattat gatgttggaa gagttcaaca
     2401 gtatatcata agaacaagca aaaacatcat tgaactgaga gacaatttta ataactctcc
     2461 tcgggttgat acaactaacc ttacaccaaa agaagccaac tccgaggaaa cctttgcctt
     2521 taaaccagaa aatatcacag aagaaaatgc aacgtacata ttcattgcca ttgaaagtgt
     2581 cgacaaaagc agtttgagtt cgggaccatc caacatcgca caagtggcac tgttcacccc
     2641 tcaggcagag cctgaccctg atgaaagtcc aagtttatca ggagttagtg ttgctactat
     2701 tgtgctgtct gttttgggcg ctcttgttct agtttgtatt attgtaggta ccacaatttg
     2761 tatcttaaag aacaaaaggt cctcatcagc agctataaca aaattttgaa agataacatg
     2821 aataaaaaag atatttttga gtttttaaag catatcccat gtcatcatga actcaaaagt
     2881 aacttcaaga gggtctaaaa aatactttaa gcacacccat tctgtgaaaa gctaccaata
     2941 ttaatattta taattttatt aacttatcat agtgataaat aaaactggtt ttattttg
//
