LOCUS       NM_178697               3951 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus chloride channel accessory 2 (Clca2), transcript
            variant 1, mRNA.
ACCESSION   NM_178697
VERSION     NM_178697.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Seltmann,K., Meyer,M., Sulcova,J., Kockmann,T., Wehkamp,U.,
            Weidinger,S., Auf dem Keller,U. and Werner,S.
  TITLE     Humidity-regulated CLCA2 protects the epidermis from hyperosmotic
            stress
  JOURNAL   Sci Transl Med 10 (440) (2018)
   PUBMED   29743348
  REMARK    GeneRIF: CLCA2 knockdown promoted keratinocyte apoptosis induced by
            hyperosmotic stress through impairment of cell-cell adhesion.
REFERENCE   3  (bases 1 to 3951)
  AUTHORS   Hughes,K., Blanck,M., Pensa,S. and Watson,C.J.
  TITLE     Stat3 modulates chloride channel accessory protein expression in
            normal and neoplastic mammary tissue
  JOURNAL   Cell Death Dis 7 (10), e2398 (2016)
   PUBMED   27711075
  REMARK    GeneRIF: Stat3-mediated regulation of expression of members of the
            calcium-activated chloride channel regulator (CLCA) family of
            proteins during involution and mammary carcinogenesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3951)
  AUTHORS   Chen,J.Q., Mori,H., Cardiff,R.D., Trott,J.F., Hovey,R.C.,
            Hubbard,N.E., Engelberg,J.A., Tepper,C.G., Willis,B.J., Khan,I.H.,
            Ravindran,R.K., Chan,S.R., Schreiber,R.D. and Borowsky,A.D.
  TITLE     Abnormal Mammary Development in 129:STAT1-Null Mice is
            Stroma-Dependent
  JOURNAL   PLoS One 10 (6), e0129895 (2015)
   PUBMED   26075897
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3951)
  AUTHORS   Hiromatsu,R., Hatta,M., Okamura,K., Sakagami,R. and Yamazaki,J.
  TITLE     NF-kappaB-regulated transcriptional control of CLCA in a
            differentiated mouse keratinocyte line
  JOURNAL   J Dermatol Sci 78 (3), 189-196 (2015)
   PUBMED   25828855
  REMARK    GeneRIF: basal NF-kappaB activity is involved in the
            Ca(2+)-dependent regulation of mCLCA2 expression in a mouse
            keratinocyte line
REFERENCE   6  (bases 1 to 3951)
  AUTHORS   Braun,J., Bothe,M.K., Mundhenk,L., Beck,C.L. and Gruber,A.D.
  TITLE     Murine mCLCA5 is expressed in granular layer keratinocytes of
            stratified epithelia
  JOURNAL   Histochem Cell Biol 133 (3), 285-299 (2010)
   PUBMED   20012443
  REMARK    GeneRIF: The results imply that mCLCA5 may play a role in
            maturation and keratinization of squamous epithelial cells.
REFERENCE   7  (bases 1 to 3951)
  AUTHORS   Piirsoo,M., Meijer,D. and Timmusk,T.
  TITLE     Expression analysis of the CLCA gene family in mouse and human with
            emphasis on the nervous system
  JOURNAL   BMC Dev Biol 9, 10 (2009)
   PUBMED   19210762
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3951)
  AUTHORS   Patel,A.C., Morton,J.D., Kim,E.Y., Alevy,Y., Swanson,S., Tucker,J.,
            Huang,G., Agapov,E., Phillips,T.E., Fuentes,M.E., Iglesias,A.,
            Aud,D., Allard,J.D., Dabbagh,K., Peltz,G. and Holtzman,M.J.
  TITLE     Genetic segregation of airway disease traits despite redundancy of
            calcium-activated chloride channel family members
  JOURNAL   Physiol Genomics 25 (3), 502-513 (2006)
   PUBMED   16569774
REFERENCE   9  (bases 1 to 3951)
  AUTHORS   Beckley,J.R., Pauli,B.U. and Elble,R.C.
  TITLE     Re-expression of detachment-inducible chloride channel mCLCA5
            suppresses growth of metastatic breast cancer cells
  JOURNAL   J Biol Chem 279 (40), 41634-41641 (2004)
   PUBMED   15292178
  REMARK    GeneRIF: results establish mCLCA5 as the mouse ortholog of hCLCA2,
            demonstrate that mCLCA5 is a detachment-sensitive growth inhibitor,
            and suggest a mechanism whereby these channels may antagonize
            mammary tumor progression, metastases [CLCA5]
REFERENCE   10 (bases 1 to 3951)
  AUTHORS   Evans,S.R., Thoreson,W.B. and Beck,C.L.
  TITLE     Molecular and functional analyses of two new calcium-activated
            chloride channel family members from mouse eye and intestine
  JOURNAL   J Biol Chem 279 (40), 41792-41800 (2004)
   PUBMED   15284223
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC152400.9.
            
            On Jul 22, 2022 this sequence version replaced NM_178697.5.
            
            Summary: This gene encodes a member of the calcium-activated
            chloride channel regulator (CLCR) family of proteins. Members of
            this family regulate the transport of chloride across the plasma
            membrane. Expression of this gene is upregulated by the tumor
            suppressor protein p53 in response to DNA damage. Mice lacking a
            functional copy of this gene exhibit increased liver weight and
            hepatocyte hypertrophy. [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096379.1, AK028704.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-292               AC152400.9         106025-106316
            293-430             AC152400.9         107200-107337
            431-581             AC152400.9         109829-109979
            582-690             AC152400.9         112868-112976
            691-850             AC152400.9         114235-114394
            851-1078            AC152400.9         116947-117174
            1079-1309           AC152400.9         118569-118799
            1310-1487           AC152400.9         119989-120166
            1488-1594           AC152400.9         120903-121009
            1595-1819           AC152400.9         123675-123899
            1820-2090           AC152400.9         126956-127226
            2091-2261           AC152400.9         129298-129468
            2262-2492           AC152400.9         132728-132958
            2493-3951           AC152400.9         133373-134831
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 69.31 cM"
     gene            1..3951
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /note="chloride channel accessory 2"
                     /db_xref="GeneID:229933"
                     /db_xref="MGI:MGI:2139758"
     exon            1..292
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     CDS             107..2935
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /note="isoform 1 is encoded by transcript variant 1;
                     calcium-activated chloride channel regulator 2;
                     calcium-activated chloride channel family member 5"
                     /codon_start=1
                     /product="calcium-activated chloride channel regulator 2
                     isoform 1"
                     /protein_id="NP_848812.1"
                     /db_xref="CCDS:CCDS17890.1"
                     /db_xref="GeneID:229933"
                     /db_xref="MGI:MGI:2139758"
                     /translation="MTHRDSTGPVIGLKLVTLLFTLSPELLFLGAGLKLKENGYDGLL
                     VAINPRVPEDLKLITNIKEMITEASFYLFNATKRRVFFRNVQILVPATWTDHNYSRVR
                     QESYDKANVIVAEQSEEHGDDPYTLQHRGCGQEGRYIHFTPSFLLNDELAAGYGARGR
                     VFVHEWAHLRWGVFDEYNNDKPFYVNGRNEIQVTRCSSDITGVFVCEKGLCPHEDCII
                     SKIFREGCTFLYNSTQNATGSIMFMPSLPSVVEFCNESTHNQEAPNLQNQVCSLRSTW
                     DVITASSDLNHSLPVHGVGLPAPPTFSLLQAGDRVVCLVIDVSRKMAEGDRLLRLQQA
                     AELYLMQVVEAHTFVGIVTFDSKGEIRASLQQIYSDDDRKLLVSYLPTAVSTDAETNI
                     CAGVKKGFEVVEERNGRADGSVLILVTSGADEHIANCLLTSMNSGSTIHSMALGSSAA
                     RKVGELSRLTGGLKFFIPDKFTSNGMTEAFVRISSGTGDIFQQSLQVESVCETVQPQH
                     QLADTMTVDSAVGNDTLFLVTWQTGGPPEIALLDPSGRKYNTGDFIINLAFRTASLKI
                     PGTAKHGHWTYTLNNTHHSPQALKVTVASRASSLAMSPATLEAFVERDSTYFPQPVII
                     YANVRKGLHPILNATVVATVEPEAGDPVVLQLLDGGAGADVIRNDGIYSRYFSSFAVS
                     GSYSLTVHVRHSPSTSTLALPVPGNHAMYVPGYITNDNIQMNAPKNLGHRPVKERWGF
                     SRVSSGGSFSVLGVPDGPHPDMFPPCKITDLEAMKVEDDVVLSWTAPGEDFDQGQTTS
                     YEIRMSRSLWNIRDDFDNAILVNSSELVPQHAGTRETFTFSPKLVTHELDHELAEDAQ
                     EPYIVYVALRAMDRSSLRSAVSNIALVSMSLPPNSSPVVSRDDLILKGVLTTVGLIAI
                     LCLIMVVAHCIFNRKKRPSRKENETKFL"
     exon            293..430
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            431..581
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            582..690
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            691..850
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            851..1078
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1309
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1487
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1594
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            1595..1819
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..2090
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            2091..2261
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            2262..2492
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     exon            2493..3951
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3839..3844
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /note="hexamer: AATAAA"
     polyA_site      3859
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /note="major polyA site"
     regulatory      3932..3937
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /note="hexamer: AATAAA"
     polyA_site      3951
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /note="major polyA site"
ORIGIN      
        1 gtaagacctt ctaagttccg aaagaagcca gctgcttcca cattgatcag ctgactcaag
       61 gtacccagca ggctccccgt gaataaacca gaggtttcac tgcaagatga cccacaggga
      121 cagcacagga cctgtcatcg ggctgaaact tgtgaccctt ctgttcaccc taagtccaga
      181 acttctgttc ctgggagctg gattgaagct gaaagagaat ggctatgatg gattgcttgt
      241 tgccatcaat ccccgggtac ccgaggatct gaagctgatt acaaacatta aggaaatgat
      301 aaccgaagct tccttttacc tgttcaatgc gaccaagagg agggtgtttt tcagaaatgt
      361 acagatttta gtacctgcca cttggacgga tcataattac agtagagtaa gacaagaatc
      421 ctatgacaag gcaaatgtca tagtggctga gcagagtgag gaacacggag atgatcccta
      481 caccctacag cacagagggt gtgggcaaga ggggagatac atccacttca cccccagctt
      541 cctactcaac gatgagttag ccgcaggcta tggtgcgcga ggcagagtgt ttgtccacga
      601 gtgggcccat ctccgctggg gtgtgttcga tgaatataac aacgacaaac cgttctacgt
      661 gaatggacgg aatgagattc aagtcaccag gtgttcatct gacattacag gcgtttttgt
      721 gtgtgaaaag ggcctctgcc cccatgaaga ctgcattatt agcaagattt ttagggaagg
      781 atgcacattc ctctacaaca gcacccagaa tgcaactgga tcaataatgt tcatgccaag
      841 tttaccttct gtggttgaat tctgtaacga aagtacacac aaccaagaag ctccaaacct
      901 ccagaaccaa gtgtgcagcc tcagaagcac ttgggacgtg atcacagcct cctccgacct
      961 gaaccatagc ctccccgtgc atggggttgg acttccagcc cctcccacgt tctccctctt
     1021 gcaggctggt gaccgagtgg tctgcttagt gattgatgtg tccaggaaga tggcagaggg
     1081 agacagactg ctccgacttc aacaagcagc cgaactgtac ttgatgcagg ttgtcgaagc
     1141 tcacactttc gtgggcattg tcacttttga tagcaaagga gaaatccgag cctcgctgca
     1201 gcaaatttac agtgacgatg accggaagct gctggtttca tacctgccga ccgccgtgtc
     1261 cactgacgca gaaacgaaca tctgtgcagg ggttaagaaa ggctttgagg tggttgagga
     1321 gcggaatgga agagctgacg gctctgtcct gatattagtg accagtggag cagatgaaca
     1381 cattgccaac tgcctgctca cctcgatgaa cagtggatcc accattcact ccatggccct
     1441 gggttcctct gcagccagaa aagtggggga attatcacgt cttacaggag gtctaaagtt
     1501 cttcattcca gataaattta cttctaatgg aatgactgaa gctttcgttc gaatctcttc
     1561 tggaacagga gacattttcc agcaaagctt acaggttgag agcgtgtgcg aaactgtgca
     1621 accccagcac cagctggcgg atactatgac tgtggatagc gccgtgggca atgacacact
     1681 ttttctagtc acgtggcaga ctggtggccc ccctgagatt gcattattgg atcctagcgg
     1741 aagaaaatac aacactggtg actttatcat caacctggcc tttcggacag ccagccttaa
     1801 gattccaggg acagctaagc atgggcactg gacttacacg ctgaacaaca cccaccattc
     1861 tccccaagct ctgaaagtga cagtggcctc tcgtgcctcc agcctggcca tgtccccagc
     1921 cactctggaa gcctttgtgg aaagagacag cacctatttt cctcagccag tgatcattta
     1981 tgcgaatgtg aggaaaggtc tgcatcccat tctcaatgcc accgtggtgg cgacagtgga
     2041 accagaggct ggagatcccg ttgtactgca acttttggat ggcggagcag gtgcagatgt
     2101 tataagaaat gatgggattt actccaggta tttttcctcc tttgctgtaa gtggtagcta
     2161 tagcctgaca gtgcatgtcc gtcactctcc cagcacaagc acactagccc tccctgtccc
     2221 aggaaaccat gctatgtatg taccaggtta cataacaaac gataatattc aaatgaatgc
     2281 tcccaaaaac ttgggccaca gacctgtgaa ggagaggtgg ggcttcagtc gagtgagctc
     2341 gggaggctcc ttctccgtgc tgggagtccc agacggcccc caccctgaca tgtttccacc
     2401 gtgcaaaatt actgacctgg aagccatgaa agtggaagac gacgtcgtcc tctcttggac
     2461 ggcacctggg gaagacttcg atcaggggca aactacaagc tatgaaataa gaatgagcag
     2521 aagcctatgg aacattcggg atgactttga caatgccatc ttggtgaatt cgtcagagct
     2581 agttcctcag catgctggca ccagggagac atttacattc tcacccaagc ttgtcaccca
     2641 tgaacttgat catgaacttg ctgaagatgc acaagaaccc tacatagtgt atgtggccct
     2701 gagagccatg gatagaagct ccctcaggtc agctgtgtca aacattgccc tggtatcaat
     2761 gtctcttcct ccaaactctt ctcctgtagt gagcagagat gatctgatcc tgaaaggagt
     2821 tttaacaaca gtaggtttga tagcaatcct ttgccttatt atggttgtag cacactgtat
     2881 ttttaacagg aaaaagagac catcaagaaa agagaatgag acaaaatttc tatgaacaag
     2941 caggcacagt atcttccttc ttaggtagga tggacatgac ctttacatcc acaaaataaa
     3001 atgtgaacaa aatcaaaata gtctcgacat ggggactttt acataatgca aaaatgccct
     3061 cccccccccc ccaaaaaaaa cccaccaact tttaactcat tttgggaaag ggttagaaaa
     3121 cagtgtaagg ttccagttat ggaaaaataa taaaatatat tgctcaaggt aatggcttga
     3181 aaggcaaagg aagaacaaaa tcaaatcgag tcaagaaaag cttgttttat tgaagttcag
     3241 gttgggggaa gttctgggta cagaaaagaa tgttgggtct tagttagaca gtgtaactat
     3301 ctgtatgatg caaacatgtt actttgatga atttctcatc tctgcttatc tgtgcagaga
     3361 aggacacatg tttatactga caaccaagct gctttataga agaggccata ctacagggtt
     3421 ctttatatct tgtcctttgg ttaaattcac tgtggctcac aagacaccac taaagttcag
     3481 ataggacttt tctcaccatg agaggagacc ttagaatgca attgttgtcc ttgtcccttg
     3541 gatactgatc tgtagcagag gtcaccggag tttactgttt gtaagacgtt agtgccattg
     3601 aagcagcttt ctaagttatt ggcttggaag tattgaatga aaatggttac ggctcccatg
     3661 aggctttaca ggtaaaagac attatgctga aataatttta atatatacac catatagttt
     3721 tattccatct caaataagaa gtcatggaaa caattagcaa attctgcccc ggtttaataa
     3781 gtacatgcaa ttcttttctt catcaaggac acagagaaga cagcagagaa aatgtggaaa
     3841 taaaaatcat ctaatgctac ttcttccttt taaaaaatgt tatttaataa tattatgtca
     3901 tttctaaatt caagaactta aaggtttatt gaataaatat tatgttacag a
//
