LOCUS       NP_848812                942 aa            linear   ROD 02-MAY-2024
DEFINITION  calcium-activated chloride channel regulator 2 isoform 1 [Mus
            musculus].
ACCESSION   NP_848812
VERSION     NP_848812.1
DBSOURCE    REFSEQ: accession NM_178697.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 942)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 942)
  AUTHORS   Seltmann,K., Meyer,M., Sulcova,J., Kockmann,T., Wehkamp,U.,
            Weidinger,S., Auf dem Keller,U. and Werner,S.
  TITLE     Humidity-regulated CLCA2 protects the epidermis from hyperosmotic
            stress
  JOURNAL   Sci Transl Med 10 (440) (2018)
   PUBMED   29743348
  REMARK    GeneRIF: CLCA2 knockdown promoted keratinocyte apoptosis induced by
            hyperosmotic stress through impairment of cell-cell adhesion.
REFERENCE   3  (residues 1 to 942)
  AUTHORS   Hughes,K., Blanck,M., Pensa,S. and Watson,C.J.
  TITLE     Stat3 modulates chloride channel accessory protein expression in
            normal and neoplastic mammary tissue
  JOURNAL   Cell Death Dis 7 (10), e2398 (2016)
   PUBMED   27711075
  REMARK    GeneRIF: Stat3-mediated regulation of expression of members of the
            calcium-activated chloride channel regulator (CLCA) family of
            proteins during involution and mammary carcinogenesis.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 942)
  AUTHORS   Chen,J.Q., Mori,H., Cardiff,R.D., Trott,J.F., Hovey,R.C.,
            Hubbard,N.E., Engelberg,J.A., Tepper,C.G., Willis,B.J., Khan,I.H.,
            Ravindran,R.K., Chan,S.R., Schreiber,R.D. and Borowsky,A.D.
  TITLE     Abnormal Mammary Development in 129:STAT1-Null Mice is
            Stroma-Dependent
  JOURNAL   PLoS One 10 (6), e0129895 (2015)
   PUBMED   26075897
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 942)
  AUTHORS   Hiromatsu,R., Hatta,M., Okamura,K., Sakagami,R. and Yamazaki,J.
  TITLE     NF-kappaB-regulated transcriptional control of CLCA in a
            differentiated mouse keratinocyte line
  JOURNAL   J Dermatol Sci 78 (3), 189-196 (2015)
   PUBMED   25828855
  REMARK    GeneRIF: basal NF-kappaB activity is involved in the
            Ca(2+)-dependent regulation of mCLCA2 expression in a mouse
            keratinocyte line
REFERENCE   6  (residues 1 to 942)
  AUTHORS   Braun,J., Bothe,M.K., Mundhenk,L., Beck,C.L. and Gruber,A.D.
  TITLE     Murine mCLCA5 is expressed in granular layer keratinocytes of
            stratified epithelia
  JOURNAL   Histochem Cell Biol 133 (3), 285-299 (2010)
   PUBMED   20012443
  REMARK    GeneRIF: The results imply that mCLCA5 may play a role in
            maturation and keratinization of squamous epithelial cells.
REFERENCE   7  (residues 1 to 942)
  AUTHORS   Piirsoo,M., Meijer,D. and Timmusk,T.
  TITLE     Expression analysis of the CLCA gene family in mouse and human with
            emphasis on the nervous system
  JOURNAL   BMC Dev Biol 9, 10 (2009)
   PUBMED   19210762
  REMARK    Publication Status: Online-Only
REFERENCE   8  (residues 1 to 942)
  AUTHORS   Patel,A.C., Morton,J.D., Kim,E.Y., Alevy,Y., Swanson,S., Tucker,J.,
            Huang,G., Agapov,E., Phillips,T.E., Fuentes,M.E., Iglesias,A.,
            Aud,D., Allard,J.D., Dabbagh,K., Peltz,G. and Holtzman,M.J.
  TITLE     Genetic segregation of airway disease traits despite redundancy of
            calcium-activated chloride channel family members
  JOURNAL   Physiol Genomics 25 (3), 502-513 (2006)
   PUBMED   16569774
REFERENCE   9  (residues 1 to 942)
  AUTHORS   Beckley,J.R., Pauli,B.U. and Elble,R.C.
  TITLE     Re-expression of detachment-inducible chloride channel mCLCA5
            suppresses growth of metastatic breast cancer cells
  JOURNAL   J Biol Chem 279 (40), 41634-41641 (2004)
   PUBMED   15292178
  REMARK    GeneRIF: results establish mCLCA5 as the mouse ortholog of hCLCA2,
            demonstrate that mCLCA5 is a detachment-sensitive growth inhibitor,
            and suggest a mechanism whereby these channels may antagonize
            mammary tumor progression, metastases [CLCA5]
REFERENCE   10 (residues 1 to 942)
  AUTHORS   Evans,S.R., Thoreson,W.B. and Beck,C.L.
  TITLE     Molecular and functional analyses of two new calcium-activated
            chloride channel family members from mouse eye and intestine
  JOURNAL   J Biol Chem 279 (40), 41792-41800 (2004)
   PUBMED   15284223
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC152400.9.
            
            Summary: This gene encodes a member of the calcium-activated
            chloride channel regulator (CLCR) family of proteins. Members of
            this family regulate the transport of chloride across the plasma
            membrane. Expression of this gene is upregulated by the tumor
            suppressor protein p53 in response to DNA damage. Mice lacking a
            functional copy of this gene exhibit increased liver weight and
            hepatocyte hypertrophy. [provided by RefSeq, Sep 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096379.1, AK028704.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 69.31 cM"
     Protein         1..942
                     /product="calcium-activated chloride channel regulator 2
                     isoform 1"
                     /note="calcium-activated chloride channel regulator 2;
                     calcium-activated chloride channel family member 5"
                     /calculated_mol_wt=103495
     Region          33..881
                     /region_name="hCaCC"
                     /note="calcium-activated chloride channel protein 1;
                     TIGR00868"
                     /db_xref="CDD:129946"
     Region          54..205
                     /region_name="Metalloprotease domain.
                     /evidence=ECO:0000250|UniProtKB:A8K7I4"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            74
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            97
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            231
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            235
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            254
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            286
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            522
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            580
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            637
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            708..709
                     /site_type="cleavage"
                     /note="Cleavage, by autolysis.
                     /evidence=ECO:0000250|UniProtKB:A8K7I4; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            821
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q8BG22.1)"
     Site            906..926
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8BG22.1)"
     CDS             1..942
                     /gene="Clca2"
                     /gene_synonym="4732440A06; Clca5"
                     /coded_by="NM_178697.6:107..2935"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS17890.1"
                     /db_xref="GeneID:229933"
                     /db_xref="MGI:MGI:2139758"
ORIGIN      
        1 mthrdstgpv iglklvtllf tlspellflg aglklkengy dgllvainpr vpedlklitn
       61 ikemiteasf ylfnatkrrv ffrnvqilvp atwtdhnysr vrqesydkan vivaeqseeh
      121 gddpytlqhr gcgqegryih ftpsfllnde laagygargr vfvhewahlr wgvfdeynnd
      181 kpfyvngrne iqvtrcssdi tgvfvcekgl cphedciisk ifregctfly nstqnatgsi
      241 mfmpslpsvv efcnesthnq eapnlqnqvc slrstwdvit assdlnhslp vhgvglpapp
      301 tfsllqagdr vvclvidvsr kmaegdrllr lqqaaelylm qvveahtfvg ivtfdskgei
      361 raslqqiysd ddrkllvsyl ptavstdaet nicagvkkgf evveerngra dgsvlilvts
      421 gadehiancl ltsmnsgsti hsmalgssaa rkvgelsrlt gglkffipdk ftsngmteaf
      481 vrissgtgdi fqqslqvesv cetvqpqhql adtmtvdsav gndtlflvtw qtggppeial
      541 ldpsgrkynt gdfiinlafr taslkipgta khghwtytln nthhspqalk vtvasrassl
      601 amspatleaf verdstyfpq pviiyanvrk glhpilnatv vatvepeagd pvvlqlldgg
      661 agadvirndg iysryfssfa vsgsysltvh vrhspststl alpvpgnham yvpgyitndn
      721 iqmnapknlg hrpvkerwgf srvssggsfs vlgvpdgphp dmfppckitd leamkveddv
      781 vlswtapged fdqgqttsye irmsrslwni rddfdnailv nsselvpqha gtretftfsp
      841 klvtheldhe laedaqepyi vyvalramdr sslrsavsni alvsmslppn sspvvsrddl
      901 ilkgvlttvg liailclimv vahcifnrkk rpsrkenetk fl
//
