LOCUS       NM_198415               1479 bp    mRNA    linear   ROD 15-OCT-2024
DEFINITION  Mus musculus creatine kinase, mitochondrial 2 (Ckmt2), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_198415 XM_127443
VERSION     NM_198415.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Rizo-Roca,D., Guimaraes,D.S.P.S.F., Pendergrast,L.A., Di Leo,N.,
            Chibalin,A.V., Maqdasy,S., Ryden,M., Naslund,E., Zierath,J.R. and
            Krook,A.
  TITLE     Decreased mitochondrial creatine kinase 2 impairs skeletal muscle
            mitochondrial function independently of insulin in type 2 diabetes
  JOURNAL   Sci Transl Med 16 (768), eado3022 (2024)
   PUBMED   39383244
  REMARK    GeneRIF: Decreased mitochondrial creatine kinase 2 impairs skeletal
            muscle mitochondrial function independently of insulin in type 2
            diabetes.
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Li,C., Zhang,Y., Shen,J., Bao,H., Zhao,Y., Li,D., Li,S., Liu,Y.,
            Yang,J., Zhou,Z., Gao,K., Zhao,L., Pei,Y., Lu,Y., Pan,Z. and Cai,B.
  TITLE     Cfp1 Controls Cardiomyocyte Maturation by Modifying Histone H3K4me3
            of Structural, Metabolic, and Contractile Related Genes
  JOURNAL   Adv Sci (Weinh) 11 (11), e2305992 (2024)
   PUBMED   38196272
REFERENCE   3  (bases 1 to 1479)
  AUTHORS   Sakamoto,T., Matsuura,T.R., Wan,S., Ryba,D.M., Kim,J.U., Won,K.J.,
            Lai,L., Petucci,C., Petrenko,N., Musunuru,K., Vega,R.B. and
            Kelly,D.P.
  TITLE     A Critical Role for Estrogen-Related Receptor Signaling in Cardiac
            Maturation
  JOURNAL   Circ Res 126 (12), 1685-1702 (2020)
   PUBMED   32212902
REFERENCE   4  (bases 1 to 1479)
  AUTHORS   Whittington,H.J., Ostrowski,P.J., McAndrew,D.J., Cao,F., Shaw,A.,
            Eykyn,T.R., Lake,H.A., Tyler,J., Schneider,J.E., Neubauer,S.,
            Zervou,S. and Lygate,C.A.
  TITLE     Over-expression of mitochondrial creatine kinase in the murine
            heart improves functional recovery and protects against injury
            following ischaemia-reperfusion
  JOURNAL   Cardiovasc Res 114 (6), 858-869 (2018)
   PUBMED   29509881
  REMARK    GeneRIF: Modest elevation of MtCK activity in the heart does not
            adversely affect cellular metabolism, mitochondrial or in vivo
            cardiac function, but modifies mPTP opening to protect against I/R
            injury and improve functional recovery.
REFERENCE   5  (bases 1 to 1479)
  AUTHORS   Spitler,K.M., Ponce,J.M., Oudit,G.Y., Hall,D.D. and Grueter,C.E.
  TITLE     Cardiac Med1 deletion promotes early lethality, cardiac remodeling,
            and transcriptional reprogramming
  JOURNAL   Am J Physiol Heart Circ Physiol 312 (4), H768-H780 (2017)
   PUBMED   28159809
REFERENCE   6  (bases 1 to 1479)
  AUTHORS   in 't Zandt,H.J., Oerlemans,F., Wieringa,B. and Heerschap,A.
  TITLE     Effects of ischemia on skeletal muscle energy metabolism in mice
            lacking creatine kinase monitored by in vivo 31P nuclear magnetic
            resonance spectroscopy
  JOURNAL   NMR Biomed 12 (6), 327-334 (1999)
   PUBMED   10516614
REFERENCE   7  (bases 1 to 1479)
  AUTHORS   Saupe,K.W., Spindler,M., Tian,R. and Ingwall,J.S.
  TITLE     Impaired cardiac energetics in mice lacking muscle-specific
            isoenzymes of creatine kinase
  JOURNAL   Circ Res 82 (8), 898-907 (1998)
   PUBMED   9576109
REFERENCE   8  (bases 1 to 1479)
  AUTHORS   Boehm,E., Veksler,V., Mateo,P., Lenoble,C., Wieringa,B. and
            Ventura-Clapier,R.
  TITLE     Maintained coupling of oxidative phosphorylation to creatine kinase
            activity in sarcomeric mitochondrial creatine kinase-deficient mice
  JOURNAL   J Mol Cell Cardiol 30 (5), 901-912 (1998)
   PUBMED   9618231
REFERENCE   9  (bases 1 to 1479)
  AUTHORS   Steeghs,K., Benders,A., Oerlemans,F., de Haan,A., Heerschap,A.,
            Ruitenbeek,W., Jost,C., van Deursen,J., Perryman,B., Pette,D.,
            Bruckwilder,M., Koudijs,J., Jap,P., Veerkamp,J. and Wieringa,B.
  TITLE     Altered Ca2+ responses in muscles with combined mitochondrial and
            cytosolic creatine kinase deficiencies
  JOURNAL   Cell 89 (1), 93-103 (1997)
   PUBMED   9094718
REFERENCE   10 (bases 1 to 1479)
  AUTHORS   Steeghs,K., Heerschap,A., de Haan,A., Ruitenbeek,W., Oerlemans,F.,
            van Deursen,J., Perryman,B., Pette,D., Bruckwilder,M., Koudijs,J.,
            Jap,P. and Wieringa,B.
  TITLE     Use of gene targeting for compromising energy homeostasis in
            neuro-muscular tissues: the role of sarcomeric mitochondrial
            creatine kinase
  JOURNAL   J Neurosci Methods 71 (1), 29-41 (1997)
   PUBMED   9125373
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167972.3.
            
            On Feb 28, 2022 this sequence version replaced NM_198415.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061221.1, SRR5189673.186879.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849390 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-61                AC167972.3         152107-152167       c
            62-233              AC167972.3         140467-140638       c
            234-432             AC167972.3         138424-138622       c
            433-528             AC167972.3         137038-137133       c
            529-750             AC167972.3         136423-136644       c
            751-836             AC167972.3         135144-135229       c
            837-960             AC167972.3         134480-134603       c
            961-1095            AC167972.3         133496-133630       c
            1096-1221           AC167972.3         131023-131148       c
            1222-1479           AC167972.3         128676-128933       c
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 47.43 cM"
     gene            1..1479
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /note="creatine kinase, mitochondrial 2"
                     /db_xref="GeneID:76722"
                     /db_xref="MGI:MGI:1923972"
     exon            1..61
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            62..233
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..1341
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /EC_number="2.7.3.2"
                     /note="creatine kinase S-type, mitochondrial; basic-type
                     mitochondrial creatine kinase; sarcomeric mitochondrial
                     creatine kinase"
                     /codon_start=1
                     /product="creatine kinase S-type, mitochondrial precursor"
                     /protein_id="NP_940807.1"
                     /db_xref="CCDS:CCDS26679.1"
                     /db_xref="GeneID:76722"
                     /db_xref="MGI:MGI:1923972"
                     /translation="MASAFSKLLTGRNASLLFTTLGTSALTTGYLLNRQKVSADAREQ
                     HKLFPPSADYPDLRKHNNCMAECLTPTIYAKLRNKMTPSGYTLDQCIQTGVDNPGHPF
                     IKTVGMVAGDEESYEVFADLFDPVIKLRHNGYDPRVMKHPTDLDASKITHGQFDERYV
                     LSSRVRTGRSIRGLSLPPACSRAERREVENVAITALEGLKGDLAGRYYKLSEMTEQDQ
                     QRLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNYDKTFLIWINEEDHTRVISMEK
                     GGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVRIP
                     KLSKDPRFSKILENLRLQKRGTGGVDTAAVADVYDISNIDRIGRSEVELVQIVIDGVN
                     YLVDCEKKLERGQDIKVPPPLPQFGRK"
     exon            234..432
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            433..528
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            529..750
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            751..836
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            837..960
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1095
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1221
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1479
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1461..1466
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /note="hexamer: AATAAA"
     polyA_site      1479
                     /gene="Ckmt2"
                     /gene_synonym="2300008A19Rik; mib-CK; S-MtCK; ScCKmit"
                     /note="major polyA site"
ORIGIN      
        1 acacttcttg gctgtgtgca cgcagcagga tctggatacg acagactcct ccagcttcaa
       61 gacacttaac caagaagaag gatggccagt gccttctcaa agttgctaac tggccgaaat
      121 gcatctctgc tgtttactac actgggaacc agtgccctga ccactggtta cctgctgaat
      181 aggcagaagg tatctgctga tgcccgggaa cagcacaagc tctttccccc aagtgcggac
      241 taccctgacc ttcgaaaaca taacaactgc atggctgagt gcctcacccc caccatctat
      301 gccaaactcc gaaacaagat gacacccagt ggctataccc tggaccagtg catccaaact
      361 ggagtggaca accccggcca ccccttcatt aagactgtgg gcatggtggc tggtgatgag
      421 gagtcgtatg aggtatttgc tgaccttttt gatcctgtca tcaaactaag gcacaatggc
      481 tatgacccta gggtgatgaa gcatcctacg gacctggatg catccaagat tactcacggg
      541 cagtttgatg agcgctatgt gttgtcctct cgggtacgca ctggccgaag catccgcggg
      601 ctgagcctgc ctccagcctg ctcccgagca gagagaaggg aggtggagaa tgtggccatt
      661 actgccctgg agggcctgaa gggcgatctg gctggccgct actacaagct gtctgagatg
      721 acggagcagg atcagcaacg tctcatcgat gaccactttc tgtttgataa gccagtgtcc
      781 cctttactaa catgtgctgg aatggcccgt gactggcctg atgccagggg aatctggcat
      841 aattatgaca agacatttct catctggata aatgaggaag accacaccag ggtgatctca
      901 atggaaaagg gaggcaatat gaaacgcgta tttgagagat tctgtcgtgg actaaaggaa
      961 gtggaacgat taatccagga acgaggctgg gagttcatgt ggaatgagcg tctgggatac
     1021 attttgactt gcccctcgaa cctcggaact ggattgcgag ctggtgtcca cgttaggatc
     1081 ccgaagctca gcaaggatcc acgcttttct aagatcttgg agaacctgcg gctccaaaag
     1141 cgtggcacag gtggtgtgga cactgctgca gtggcagatg tgtacgatat ttccaacata
     1201 gatcggatcg gcagatcaga ggttgagctt gttcagattg ttattgacgg agtcaactac
     1261 ctggtggatt gtgaaaagaa gctggagaga ggccaagaca tcaaggtgcc ccctcctctg
     1321 cctcagtttg gcaggaagtg aattttatcc ctccaagttt atgggtaaac tgtctgctgg
     1381 tatgacagac acaggaagtc tctgctctga gagtttttgt agacttgaac atgtaaaatt
     1441 gtaggtattg ctatctttac aataaaactc tccttaata
//
