LOCUS       NM_001416952            1607 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus creatine kinase, mitochondrial 1, ubiquitous (Ckmt1),
            transcript variant 3, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001416952 XM_006498656
VERSION     NM_001416952.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Wang,S., Song,Y., Wang,Z., Chang,X., Wu,H., Yan,Z., Wu,J., He,Z.,
            Kang,L., Hu,W., Xia,T., Li,Z., Ren,X. and Bai,Y.
  TITLE     Neutrophil-derived PAD4 induces citrullination of CKMT1 exacerbates
            mucosal inflammation in inflammatory bowel disease
  JOURNAL   Cell Mol Immunol 21 (6), 620-633 (2024)
   PUBMED   38720063
  REMARK    GeneRIF: Neutrophil-derived PAD4 induces citrullination of CKMT1
            exacerbates mucosal inflammation in inflammatory bowel disease.
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Politis-Barber,V., Petrick,H.L., Raajendiran,A., DesOrmeaux,G.J.,
            Brunetta,H.S., Dos Reis,L.M., Mori,M.A., Wright,D.C., Watt,M.J. and
            Holloway,G.P.
  TITLE     Ckmt1 is Dispensable for Mitochondrial Bioenergetics Within
            White/Beige Adipose Tissue
  JOURNAL   Function (Oxf) 3 (5), zqac037 (2022)
   PUBMED   37954502
  REMARK    GeneRIF: Ckmt1 is Dispensable for Mitochondrial Bioenergetics
            Within White/Beige Adipose Tissue.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1607)
  AUTHORS   Fan,H., Lv,P., Mu,T., Zhao,X., Liu,Y., Feng,Y., Lv,J., Liu,M. and
            Tang,H.
  TITLE     LncRNA n335586/miR-924/CKMT1A axis contributes to cell migration
            and invasion in hepatocellular carcinoma cells
  JOURNAL   Cancer Lett 429, 89-99 (2018)
   PUBMED   29753758
  REMARK    GeneRIF: Mechanistic studies showed lncRNA n335586 promoted
            hepatocellular carcinoma (HCC) cells migration and invasion through
            facilitating the expression of its host gene mitochondrial creatine
            kinase (CKMT1A) by competitively binding miR-924.
            Erratum:[Cancer Lett. 2020 Dec 1;494:84-87. doi:
            10.1016/j.canlet.2020.07.018.. PMID: 32911100]
REFERENCE   4  (bases 1 to 1607)
  AUTHORS   Tachikawa,M., Watanabe,M., Fukaya,M., Sakai,K., Terasaki,T. and
            Hosoya,K.I.
  TITLE     Cell-Type-Specific Spatiotemporal Expression of Creatine
            Biosynthetic Enzyme S-adenosylmethionine:guanidinoacetate
            N-methyltransferase in Developing Mouse Brain
  JOURNAL   Neurochem Res 43 (2), 500-510 (2018)
   PUBMED   29209878
REFERENCE   5  (bases 1 to 1607)
  AUTHORS   Miotto,P.M. and Holloway,G.P.
  TITLE     In the absence of phosphate shuttling, exercise reveals the in vivo
            importance of creatine-independent mitochondrial ADP transport
  JOURNAL   Biochem J 473 (18), 2831-2843 (2016)
   PUBMED   27402793
REFERENCE   6  (bases 1 to 1607)
  AUTHORS   Zuberi,A.R., Nguyen,H.Q., Auman,H.J., Taylor,B.A. and
            Roopenian,D.C.
  TITLE     A genetic linkage map of mouse chromosome 2 extending from
            thrombospondin to paired box gene 1, including the H3 minor
            histocompatibility complex
  JOURNAL   Genomics 33 (1), 75-84 (1996)
   PUBMED   8617512
REFERENCE   7  (bases 1 to 1607)
  AUTHORS   Steeghs,K., Oerlemans,F. and Wieringa,B.
  TITLE     Mice deficient in ubiquitous mitochondrial creatine kinase are
            viable and fertile
  JOURNAL   Biochim Biophys Acta 1230 (3), 130-138 (1995)
   PUBMED   7619831
REFERENCE   8  (bases 1 to 1607)
  AUTHORS   Steeghs,K., Peters,W., Bruckwilder,M., Croes,H., Van Alewijk,D. and
            Wieringa,B.
  TITLE     Mouse ubiquitous mitochondrial creatine kinase: gene organization
            and consequences from inactivation in mouse embryonic stem cells
  JOURNAL   DNA Cell Biol 14 (6), 539-553 (1995)
   PUBMED   7598809
REFERENCE   9  (bases 1 to 1607)
  AUTHORS   Chiannilkulchai,N., Pasturaud,P., Richard,I., Auffray,C. and
            Beckmann,J.S.
  TITLE     A primary expression map of the chromosome 15q15 region containing
            the recessive form of limb-girdle muscular dystrophy (LGMD2A) gene
  JOURNAL   Hum Mol Genet 4 (4), 717-725 (1995)
   PUBMED   7633422
REFERENCE   10 (bases 1 to 1607)
  AUTHORS   Steeghs,K., Merkx,G. and Wieringa,B.
  TITLE     The ubiquitous mitochondrial creatine kinase gene maps to a
            conserved region on human chromosome 15q15 and mouse chromosome 2
            bands F1-F3
  JOURNAL   Genomics 24 (1), 193-195 (1994)
   PUBMED   7896282
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845466.4.
            
            On Feb 23, 2023 this sequence version replaced XM_006498656.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.125421.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               AL845466.4         64427-64599
            174-379             AL845466.4         65447-65652
            380-578             AL845466.4         66230-66428
            579-674             AL845466.4         66596-66691
            675-896             AL845466.4         67207-67428
            897-982             AL845466.4         67554-67639
            983-1106            AL845466.4         67757-67880
            1107-1241           AL845466.4         69326-69460
            1242-1367           AL845466.4         69626-69751
            1368-1607           AL845466.4         70149-70388
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.37 cM"
     gene            1..1607
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /note="creatine kinase, mitochondrial 1, ubiquitous"
                     /db_xref="GeneID:12716"
                     /db_xref="MGI:MGI:99441"
     exon            1..173
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            174..379
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..1484
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /EC_number="2.7.3.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 3; creatine kinase U-type, mitochondrial;
                     ubiquitous mitochondrial creatine kinase; acidic-type
                     mitochondrial creatine kinase"
                     /codon_start=1
                     /product="creatine kinase U-type, mitochondrial isoform 1
                     precursor"
                     /protein_id="NP_001403881.1"
                     /db_xref="GeneID:12716"
                     /db_xref="MGI:MGI:99441"
                     /translation="MAGPFSRLLSARPGLRLLALAGAGSLTAGILLRPESVGAAAAER
                     RRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPF
                     IKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYV
                     LSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQ
                     QQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEK
                     GGNMKRVFERFCRGLKEVEKLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLP
                     LLSKDNRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVN
                     YLIDCERRLERGQDIRIPPPLVHSKH"
     exon            380..578
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            579..674
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            675..896
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            897..982
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1106
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1241
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1367
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1607
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1583..1588
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /note="hexamer: AGTAAA"
     polyA_site      1607
                     /gene="Ckmt1"
                     /gene_synonym="mi-CK; mia-CK; Mt-CK; ScCKmit; U-MtCK;
                     UbCKmit"
                     /note="major polyA site"
ORIGIN      
        1 agctgcagag aagaaggaag agaaggaaga agaaactcac cattccctgc ccaaccccag
       61 ccccacaccc agagcaatca acaggtaggc tttcagacac tactcaggaa gagtccttcg
      121 aacagcaaac agatttaagc ctggaaaaga agaagagaaa taaaagatca aagttcctga
      181 cttgcgccag atcctgctct gaatctttgt agccgcatcc ccaagccatg gctggtccct
      241 tctcccgtct gctgtctgcc cgccctggac tcaggctcct ggctttggct ggagctgggt
      301 ctctcaccgc cgggattctg ctccgcccgg aatctgtagg agctgccgct gctgaacgga
      361 ggagactgta tcccccgagc gctgagtacc cagacctccg aaagcacaac aactgcatgg
      421 ccagtcacct gaccccagca gtctatgcac ggctctgcga caagaccaca cccactggtt
      481 ggacactaga tcagtgcatc cagactggag tggacaaccc tggccacccc ttcatcaaga
      541 ctgtgggcat ggtggctgga gatgaggaga cctatgaggt atttgctgaa ctgtttgacc
      601 ctgtgatcca agagcggcat aatggatatg accccagaac aatgaagcac accactgacc
      661 ttgatgccag taaaatccgt tctggctact ttgatgagag gtatgtattg tcttcaagag
      721 tcagaactgg ccgaagtatc aggggactca gtctccctcc agcctgcact cgggcagagc
      781 gaagagaggt agaacgtgtt gtggtggatg ctctgagtgg cctgaagggt gacctggctg
      841 gacggtacta taggctcagt gagatgacgg aggccgaaca gcagcagctt attgatgacc
      901 attttctgtt tgataaacct gtgtccccat tgctgactgc agcaggaatg gctcgagact
      961 ggcctgatgc tcgagggatc tggcacaaca atgagaagag tttcttgatc tgggtgaatg
     1021 aggaggacca cacacgggtc atctctatgg agaaaggcgg caacatgaag agagtgtttg
     1081 aaagattctg ccggggcctc aaagaggtgg agaagctgat ccaggaacga ggctgggagt
     1141 tcatgtggaa tgagcgttta ggctacatct tgacctgtcc atctaacctg ggcactggac
     1201 ttcgggcagg agtccacatc aaactgccac tgctgagcaa agataaccgc ttcccaaaga
     1261 tcctggagaa cctaagactg caaaagcgtg gaactggagg agtggacaca gctgccacag
     1321 gcagcgtctt tgacatctct aatttggatc gacttggcaa gtcagaggtg gagctggtgc
     1381 agctcgtcat cgatggggtg aactatttga ttgactgtga acggcgtctg gagagaggac
     1441 aggatattcg aatccctcca cctcttgtcc acagcaaaca ttaactcccc atccccagcg
     1501 gatgagtcaa gacccccagg acttctgcat atttaacagt ggcctagtct acttgccctg
     1561 gacctgcctc tctccctgtc ctagtaaaga ctccacatta tgttgca
//
