LOCUS       NM_181589               3154 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cytoskeleton associated protein 2-like (Ckap2l), mRNA.
ACCESSION   NM_181589
VERSION     NM_181589.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3154)
  AUTHORS   Ulmke,P.A., Sakib,M.S., Ditte,P., Sokpor,G., Kerimoglu,C., Pham,L.,
            Xie,Y., Mao,X., Rosenbusch,J., Teichmann,U., Nguyen,H.P.,
            Fischer,A., Eichele,G., Staiger,J.F. and Tuoc,T.
  TITLE     Molecular Profiling Reveals Involvement of ESCO2 in Intermediate
            Progenitor Cell Maintenance in the Developing Mouse Cortex
  JOURNAL   Stem Cell Reports 16 (4), 968-984 (2021)
   PUBMED   33798452
REFERENCE   4  (bases 1 to 3154)
  AUTHORS   Naiman,N., Fujioka,K., Fujino,M., Valerius,M.T., Potter,S.S.,
            McMahon,A.P. and Kobayashi,A.
  TITLE     Repression of Interstitial Identity in Nephron Progenitor Cells by
            Pax2 Establishes the Nephron-Interstitium Boundary during Kidney
            Development
  JOURNAL   Dev Cell 41 (4), 349-365 (2017)
   PUBMED   28535371
REFERENCE   5  (bases 1 to 3154)
  AUTHORS   Hussain,M.S., Battaglia,A., Szczepanski,S., Kaygusuz,E.,
            Toliat,M.R., Sakakibara,S., Altmuller,J., Thiele,H., Nurnberg,G.,
            Moosa,S., Yigit,G., Beleggia,F., Tinschert,S., Clayton-Smith,J.,
            Vasudevan,P., Urquhart,J.E., Donnai,D., Fryer,A., Percin,F.,
            Brancati,F., Dobbie,A., Smigiel,R., Gillessen-Kaesbach,G.,
            Wollnik,B., Noegel,A.A., Newman,W.G. and Nurnberg,P.
  TITLE     Mutations in CKAP2L, the human homolog of the mouse Radmis gene,
            cause Filippi syndrome
  JOURNAL   Am J Hum Genet 95 (5), 622-632 (2014)
   PUBMED   25439729
REFERENCE   6  (bases 1 to 3154)
  AUTHORS   Yumoto,T., Nakadate,K., Nakamura,Y., Sugitani,Y.,
            Sugitani-Yoshida,R., Ueda,S. and Sakakibara,S.
  TITLE     Radmis, a novel mitotic spindle protein that functions in cell
            division of neural progenitors
  JOURNAL   PLoS One 8 (11), e79895 (2013)
   PUBMED   24260314
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3154)
  AUTHORS   Kumada,T., Yamanaka,Y., Kitano,A., Shibata,M., Awaya,T., Kato,T.,
            Okawa,K., Abe,T., Oshima,N., Nakahata,T. and Heike,T.
  TITLE     Ttyh1, a Ca(2+)-binding protein localized to the endoplasmic
            reticulum, is required for early embryonic development
  JOURNAL   Dev Dyn 239 (8), 2233-2245 (2010)
   PUBMED   20568244
REFERENCE   8  (bases 1 to 3154)
  AUTHORS   Kuninger,D., Kuzmickas,R., Peng,B., Pintar,J.E. and Rotwein,P.
  TITLE     Gene discovery by microarray: identification of novel genes induced
            during growth factor-mediated muscle cell survival and
            differentiation
  JOURNAL   Genomics 84 (5), 876-889 (2004)
   PUBMED   15475267
REFERENCE   9  (bases 1 to 3154)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772347.6, BC053443.1, CV560846.1, CX205381.1 and CO042920.1.
            
            On Aug 15, 2009 this sequence version replaced NM_181589.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053443.1, SRR7345562.4112039.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849380,
                                           SAMN00849382 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                AL772347.6         128062-128109       c
            49-2466             BC053443.1         1-2418
            2467-2642           CV560846.1         500-675
            2643-3108           CX205381.1         181-646
            3109-3154           CO042920.1         1-46                c
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 62.87 cM"
     gene            1..3154
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /note="cytoskeleton associated protein 2-like"
                     /db_xref="GeneID:70466"
                     /db_xref="MGI:MGI:1917716"
     exon            1..108
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..2309
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /note="radial fiber and mitotic spindle protein"
                     /codon_start=1
                     /product="cytoskeleton-associated protein 2-like"
                     /protein_id="NP_853620.1"
                     /db_xref="CCDS:CCDS16724.1"
                     /db_xref="GeneID:70466"
                     /db_xref="MGI:MGI:1917716"
                     /translation="MVGPGPTASAAAEERWQKLQEYLAAKGKLKDPNAKPYLKAKNIC
                     PKPPPSKYTPGPKKDVSNHVLPSKTTRPINIKFQTKPASITASQKPESKPPKLPSRGL
                     TSRCFSSNTDCKQSSKPQQQPRAVSFTAGLSRNPRQCPDIQELKTKQQQQAHGGNAKC
                     THPETNTHAAKQPVDGFPDETNKENLPQALPKPEKPDPELHSIRKPNTGSSNQTQKGL
                     APKQILSKSSVTQTALKDRANKQFIRNTQIRTQAVKSRPRPTVADSTRPREKPPQTAP
                     SHSVPAHNKTQTSKKPMTKNTQDITVNRVRYGKPNETKIESCPATEQKVKHTKPSSQL
                     NVLQGGHNSRHPNMRQDQKPVQPHLGPQTSCVLQKSRAISQRPNLTARNFNSVIPSTP
                     NMRANKTLNNKYNNIFQQKAQTLDSKFRKFPPQSHFLNKTAPRTQASTAAASRKGAPS
                     ATQTHPHGKKPEGEDRRKQLEEWQKSKGKTYKRPPMKFKTKRKVIEEMNTSFWKSIER
                     EEEEKKAQLELSKKIDSTLTECLRLIEEGVLPNEIFTIVSSIPEAEKFAKFWVCKAKL
                     LASKGTFDAIGLYEEAIQNGATPVQELQEVLNVLQDPCRSTEAVTSDTSAAGTNTTSA
                     EELAKEESEQPCPSLTEMEPIAAAAPRIPVSEWDNHGIKLQVAPIPRICGMPEVQDMK
                     LITPVRRSARIERTVARYPEMLQEHDVVVASLNELLEVDKTECFIFRENEALPVTLGF
                     EVLES"
     exon            109..175
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     exon            176..227
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     exon            228..1471
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1679
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1835
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..1896
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..2083
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..3137
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3113..3118
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /note="hexamer: AATAAA"
     polyA_site      3137
                     /gene="Ckap2l"
                     /gene_synonym="2010016H04Rik; 2610318C08Rik; radmis; rdm"
                     /note="major polyA site"
ORIGIN      
        1 cagacgggcg cccggttctc gtactagaac aaggcgcgcc ctttgaagga agaaactgtc
       61 gccgtagagc catggtgggg cccggcccca ctgccagcgc agccgcggaa gaacggtggc
      121 agaagctcca ggagtacctg gcagccaagg gaaagctaaa ggaccccaac gccaagcctt
      181 atctaaaagc caagaatatc tgccccaaac caccaccatc taaatatact cctggaccta
      241 aaaaagatgt ttccaatcat gttttgccta gcaaaactac aagacccatc aacattaaat
      301 tccagactaa accagccagt atcacagcat cccagaagcc agagtcgaag ccaccaaaac
      361 ttccaagcag aggactcacg tcaagatgtt tttcttctaa cacagactgt aaacagtcca
      421 gcaagcctca gcagcaacct agagcagtgt cattcactgc aggactgtca agaaacccca
      481 ggcagtgccc tgatatacag gaactaaaaa ccaaacagca acagcaagca catggaggaa
      541 atgctaaatg tacacatccc gagactaaca cccacgctgc aaagcaacct gtggatggct
      601 ttccagatga aacaaacaaa gaaaacttgc cccaggccct gccaaaacct gagaagccag
      661 atcctgagtt acattctata agaaagccaa atactggctc ttctaatcaa actcagaaag
      721 gtctggctcc taaacagatc ttgagcaaaa gttcagttac tcagactgct ctgaaagaca
      781 gagctaacaa acagttcatt cgaaacacac aaatcagaac tcaagcagtg aagtctcggc
      841 cacgccctac agttgcagat tctacacgac caagagaaaa acccccacaa acagctccct
      901 ctcattccgt tccagcccat aataagactc agacatcaaa gaaaccaatg accaagaaca
      961 cacaggacat aacggttaac agggttagat acggaaaacc aaatgaaact aagatagagt
     1021 catgccctgc tactgaacag aaagtaaaac acactaaacc tagttcccaa ctcaatgtgc
     1081 ttcagggagg acataacagt agacatccca acatgagaca ggatcagaag cctgtacagc
     1141 ctcatcttgg accccagacg tcatgcgttc tgcaaaaatc aagagccata agccagaggc
     1201 ctaatttgac agctagaaac tttaattcag tcatcccaag cacgcctaac atgagagcaa
     1261 ataaaacact taacaataag tacaataaca tctttcaaca aaaggcacag acattggact
     1321 ccaagttcag aaagtttcct ccccagagcc attttttgaa caagactgct cccaggactc
     1381 aagccagtac agcagccgca agtaggaagg gagccccaag tgcgacccag actcacccac
     1441 atggtaaaaa accagaagga gaggatcgga gaaaacagct ggaagaatgg cagaaatcca
     1501 aagggaaaac ctataaacga cctcccatga aatttaaaac aaaaagaaaa gtcatagagg
     1561 aaatgaacac ttccttctgg aagagcattg aaagagaaga agaagaaaag aaagctcagc
     1621 tggagctgtc caagaaaatc gacagcactc tgacagagtg tctgaggctc attgaagagg
     1681 gtgtacttcc taatgaaata ttcaccatag tatccagtat tcctgaggct gaaaaatttg
     1741 ccaagttctg ggtgtgcaaa gcaaagttac tggcaagcaa aggcaccttt gatgctattg
     1801 gactatacga agaggccatt cagaacgggg caacaccagt acaggagctg caggaagttc
     1861 tgaacgttct acaagaccca tgcaggagca cagaagcagt aacctctgac acctcagctg
     1921 ctggaactaa taccacatct gcagaggagc tggccaagga ggaatctgag cagccttgcc
     1981 catctctaac agaaatggag cctattgcag cagcagcacc gcggatacct gtttcagagt
     2041 gggataacca tggtatcaaa ttacaggttg ctccgatccc tagaatatgc gggatgccag
     2101 aagtgcaaga catgaagctt atcactcccg tgaggcgctc agccaggatc gagcgcacgg
     2161 tggctcgcta cccagaaatg ctgcaggagc atgatgtggt ggtggcttct cttaatgagc
     2221 tactggaagt ggacaaaaca gagtgtttca tcttccgtga aaatgaggct ttgcctgtga
     2281 cactgggctt tgaagtcctt gaatcatgat cttttgatat ttgtgggttt tgttttgttt
     2341 ttttaacttt aagtgaggtt tttttcagag cttgcaagac ataaatgccc ttgtcaagat
     2401 gacatgaaat aaatgtttgc tgagacctca ccatgcaggt ggtagactct ttggtctcag
     2461 aaggaatgca ttgagtttac tctcagattg tatatcccca tgagaaggct tttggttcat
     2521 gtatttgtta tatactttat aaaagtttta tagcatagtt tttcagcatc cacactacct
     2581 ggagggcagg cttctagagt acaaagggtt gacttttgac accagctcac caggcggaaa
     2641 gtcaaactcc acagattaca gagacgtttg ggctagccac atcttcactc agatcccaga
     2701 aggtatttgt ggagaccttc agcttctacc acagtgaccc taaagatgag ggggacgctt
     2761 gtcctctctg ggtggctttc ctctagtgaa gcacagtcca gaattgaggt ggcagcccag
     2821 gttttattta cagtcctcct tttgcacttg ttattgagaa atgcttcgag taaagacaaa
     2881 tatggggaca gagcctgctg cgtccattcc tctccctacc agaggactgg tagcctgagg
     2941 aggccacgag caccgcagag actatggcag gggatctttt ctgcaccttg gacaactgct
     3001 cctggaaagg aaaagcagcg ttgttttgta aatgtaacat aggaaagcct ggttggcaat
     3061 ttttaaacct tagctttgtt cctatgatta ctaagtacta agtaatatct aaaataaaaa
     3121 ccactgaatt ctcttcaaaa aaaaaaaaaa aaaa
//
