LOCUS       NM_001004140            2601 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus cytoskeleton associated protein 2 (Ckap2), mRNA.
ACCESSION   NM_001004140 XM_134100
VERSION     NM_001004140.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   McAlear,T.S. and Bechstedt,S.
  TITLE     The mitotic spindle protein CKAP2 potently increases formation and
            stability of microtubules
  JOURNAL   Elife 11, e72202 (2022)
   PUBMED   35029146
  REMARK    GeneRIF: The mitotic spindle protein CKAP2 potently increases
            formation and stability of microtubules.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Yoo,B.H., Kang,D.S., Park,C.H., Kang,K. and Bae,C.D.
  TITLE     CKAP2 phosphorylation by CDK1/cyclinB1 is crucial for maintaining
            centrosome integrity
  JOURNAL   Exp Mol Med 49 (7), e354 (2017)
   PUBMED   28706298
  REMARK    GeneRIF: The results suggest that the phosphorylation of
            Cytoskeleton Associated Protein 2 (CKAP2) at T603 and possibly S608
            phosphorylation sites by Cyclin Dependent Kinase 1 (CDK1) during
            mitosis is essential for maintaining the number of centrosomes.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2601)
  AUTHORS   Tran,J.R., Zheng,X. and Zheng,Y.
  TITLE     Lamin-B1 contributes to the proper timing of epicardial cell
            migration and function during embryonic heart development
  JOURNAL   Mol Biol Cell 27 (25), 3956-3963 (2016)
   PUBMED   27798236
  REMARK    GeneRIF: we found that Lb1-null epicardial cells have a delayed
            nuclear morphology change in vivo, suggesting that Lb1 facilitates
            morphological changes associated with migration. These findings
            suggest that Lb1 contributes to nuclear shape maintenance and
            migration of epicardial cells
REFERENCE   4  (bases 1 to 2601)
  AUTHORS   Yoo,B.H., Park,C.H., Kim,H.J., Kang,D.S. and Bae,C.D.
  TITLE     CKAP2 is necessary to ensure the faithful spindle bipolarity in a
            dividing diploid hepatocyte
  JOURNAL   Biochem Biophys Res Commun 473 (4), 886-893 (2016)
   PUBMED   27055594
  REMARK    GeneRIF: These data suggest a physiological role of CKAP2 in the
            formation of spindle bipolarity, which is necessary for maintaining
            chromosomal stability.
REFERENCE   5  (bases 1 to 2601)
  AUTHORS   Ray,J.P., Staron,M.M., Shyer,J.A., Ho,P.C., Marshall,H.D.,
            Gray,S.M., Laidlaw,B.J., Araki,K., Ahmed,R., Kaech,S.M. and
            Craft,J.
  TITLE     The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling,
            Differentiation, and Metabolism of T Helper 1 and Follicular B
            Helper T Cells
  JOURNAL   Immunity 43 (4), 690-702 (2015)
   PUBMED   26410627
REFERENCE   6  (bases 1 to 2601)
  AUTHORS   Tsuchihara,K., Lapin,V., Bakal,C., Okada,H., Brown,L.,
            Hirota-Tsuchihara,M., Zaugg,K., Ho,A., Itie-Youten,A.,
            Harris-Brandts,M., Rottapel,R., Richardson,C.D., Benchimol,S. and
            Mak,T.W.
  TITLE     Ckap2 regulates aneuploidy, cell cycling, and cell death in a
            p53-dependent manner
  JOURNAL   Cancer Res 65 (15), 6685-6691 (2005)
   PUBMED   16061649
  REMARK    GeneRIF: Data suggest the existence of a functional positive
            feedback loop in which Ckap2 activates the G1 tetraploidy
            checkpoint and prevents aneuploidy.
REFERENCE   7  (bases 1 to 2601)
  AUTHORS   Jin,Y., Murakumo,Y., Ueno,K., Hashimoto,M., Watanabe,T.,
            Shimoyama,Y., Ichihara,M. and Takahashi,M.
  TITLE     Identification of a mouse cytoskeleton-associated protein, CKAP2,
            with microtubule-stabilizing properties
  JOURNAL   Cancer Sci 95 (10), 815-821 (2004)
   PUBMED   15504249
  REMARK    GeneRIF: These findings suggest that CKAP2 is a new MAP with
            microtubule-stabilizing properties
REFERENCE   8  (bases 1 to 2601)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2601)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 2601)
  AUTHORS   Udina,I.G., Baranova,A.V., Kompaniitsev,A.A. and Sulimova,G.E.
  TITLE     [Evolutionarily-conserved gene CKAP2,located in region 13q14.3 of
            the human genome, is frequently rearranged in various tumors]
  JOURNAL   Genetika 37 (1), 120-123 (2001)
   PUBMED   11234418
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC117665.6, AK132463.1 and BM217153.2.
            
            On Nov 20, 2009 this sequence version replaced NM_001004140.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY692438.1, AK132463.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AC117665.6         153168-153182       c
            16-2352             AK132463.1         4-2340
            2353-2601           BM217153.2         1-249               c
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 11.13 cM"
     gene            1..2601
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /note="cytoskeleton associated protein 2"
                     /db_xref="GeneID:80986"
                     /db_xref="MGI:MGI:1931797"
     exon            1..136
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..2022
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /codon_start=1
                     /product="cytoskeleton-associated protein 2"
                     /protein_id="NP_001004140.2"
                     /db_xref="CCDS:CCDS22170.1"
                     /db_xref="GeneID:80986"
                     /db_xref="MGI:MGI:1931797"
                     /translation="MAESRKRFLGRAARNPLPVTRDLQLPPTRRDQPAFREQRKQKLK
                     EYLLIRKTVFPYKQENQISRDQKMITSEDRVQEGKKVVKLKTEVADKENIESTVEKNC
                     IPLKAGEVTSSEIHNSKDNVQAVQLLSTRDDLPGQTVTLDPACHHKDNKKMQMTAEKP
                     KQDSNVSKKRVLGYYHGQIVQSKINSFRKLPSVKGESLTTTKKLPTTVSKAMKAQSEP
                     ANTVSVKASTTAAATKFADAKPVSTASKDTLVRPPIRSLHSSSHGAAKQGLSRPLANV
                     TVRKGMLDKESHRSEPVVSVVKAGSSQAPSRSIASKDAARTDSSNTRLMVKPKDTDQR
                     RYTIAGASVHRSAQLKDTTAERKAQMTEWRTGKGKGLKRPPHSVVTQAEPKGQSENPV
                     GSFWTTMAEEDEQRLFTEKVNKTISECLNLINEGCPKEEILATLNDLIHNIPDAKKLV
                     KYWICLVRIEPITSPIENIISIYEKAILAGAQPIEEMRHIIIDILTTKSQEKVNLGEN
                     IEEAHATKEPIQEVNADANVGSGKPGEENEHHGKVEVYEDDQDNKIKDPDLTTPDSKT
                     EAGCIIRYNVSSTPRLQSMKKMQHDKNSTLKELKFLTPVRRSRRIQDKTSRLPAMLKD
                     HDPSVSSLEQLSELGGDAFVCRPNAALCPLFFETDVAEEE"
     exon            137..218
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     exon            219..291
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     exon            292..1097
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1098..1302
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1473
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1543
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1778
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..2601
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2282..2287
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /note="hexamer: AATATA"
     polyA_site      2304
                     /gene="Ckap2"
                     /gene_synonym="LB1"
                     /note="major polyA site"
ORIGIN      
        1 ccggcgccga ggcgcgctca ttaagcgatg gcagagtcca ggaaacgctt ccttggcagg
       61 gctgcgagga atcccttgcc ggtgacccgg gacctgcagc tgcccccgac caggcgagac
      121 cagccggcct tccgagaaca aagaaaacaa aaactcaagg aatatctgtt aatacgaaaa
      181 acagtgtttc catacaaaca agaaaatcag atatccagag atcagaaaat gataacatct
      241 gaggatcggg tccaagaagg gaaaaaagtt gtgaaactca aaacagaagt ggctgataaa
      301 gaaaatattg aaagtacagt tgagaaaaac tgcattccat taaaagctgg agaagtaacc
      361 agctcagaaa tacataattc aaaggataat gttcaagctg tgcagttatt atctacaaga
      421 gatgatttgc caggtcagac tgtgacatta gacccggcat gtcaccataa agacaataaa
      481 aagatgcaaa tgactgcaga gaagcccaaa caagacagta atgtgtctaa aaagcgagtg
      541 cttggctatt atcatggcca gattgttcag tctaagatta attcatttag aaagcttccc
      601 agtgtcaaag gcgagagtct tacaacaacc aagaaacttc ccactactgt gtctaaagcc
      661 atgaaggctc agagtgagcc tgcgaacacc gtcagcgtaa aagcctcaac tacagctgct
      721 gccaccaagt ttgcagacgc taaacctgtg agcactgcgt cgaaggacac acttgtgcga
      781 ccacctatcc gaagcctcca cagtagttcc cacggagctg cgaaacaggg cctcagcagg
      841 ccgcttgcca atgttacagt cagaaaaggg atgcttgaca aggagtcaca tcgatcagaa
      901 ccagttgtgt ccgttgtcaa agccggttct tctcaggccc catcaagaag catagcatct
      961 aaagatgcag ccaggactga ttcgtctaat accagactga tggtaaagcc aaaagatact
     1021 gaccagcgca gatacacgat tgcaggagca agtgttcata gatcagctca gctcaaggac
     1081 accaccgcag agaggaaagc tcagatgacg gagtggagaa ctggcaaagg aaaggggcta
     1141 aaacgacctc ctcattcagt agtcacccag gctgagccaa agggacaaag tgaaaaccca
     1201 gttgggtcct tttggactac catggcagaa gaagatgagc agagattatt tactgaaaaa
     1261 gtaaacaaaa ctatttctga atgcctgaac cttattaatg agggatgccc aaaagaagaa
     1321 attttggcta cattgaatga cctgattcat aatattccag atgccaaaaa acttgttaaa
     1381 tattggatat gtcttgtacg tattgaacca atcacaagtc ctattgaaaa tattatctca
     1441 atctatgaga aggccatcct ggcaggggct cagcctattg aagagatgcg tcatataatc
     1501 atagatattc taacaacgaa gagtcaagaa aaagtcaatt tgggagaaaa tattgaggag
     1561 gctcatgcaa ccaaggaacc tatccaagaa gtaaatgctg atgctaatgt agggtcagga
     1621 aaaccaggag aggagaatga acatcatggc aaagttgagg tgtatgaaga tgatcaagac
     1681 aacaaaataa aagatccgga tttaacaact ccggactcaa aaactgaagc aggttgcata
     1741 attcgatata atgtatcttc tacacctcgg ctgcaaagta tgaaaaagat gcaacatgat
     1801 aaaaattcca cacttaaaga gctaaagttc ctgacaccag tgagacgctc acggcgcatt
     1861 caagacaaga cttcacgact gccagccatg ttaaaagacc acgacccttc tgtgtcttcc
     1921 ctggagcagt tgtctgagtt gggaggggat gcctttgtct gccgccctaa tgcagcactg
     1981 tgccctctgt tctttgagac cgacgtagcg gaagaggaat aggagtcccg agtcctgagc
     2041 agttatggtt gtgttactcc tgtggccctg catgtgtaca actttatcgt aggtctgaag
     2101 ttgtccgcat gcctaagtat ggtacaggta aactccacta gcttgtttgt tgtgttttta
     2161 aaatataatg ttggctgctt cactttacta attacttaca gttaaagaaa caaaatcaga
     2221 aaatgtagtc tatataaatt tatattttta cgttgacttt gttcctaata cgtttataat
     2281 aaatatatat gtataatgtt ccaatgacat ttcattgatt gacttatttg atgatggaat
     2341 tgactttagg accctactgt ggggctatag ttagctgcct atggcattta tttcaacaaa
     2401 tatgttctgg ggtctcagga tgtgccccca gaagagtgct tgcctaatgt gcaaggctct
     2461 gaattcagtc cttagcacca caagaaaaac tgctttgtaa aatgtcattt aaccacagta
     2521 accaaattta aacttagttt ttgaagtata ttgctaagtt tgtattactt ttagttgtga
     2581 taaaatatac ctgacagaaa c
//
