LOCUS       NM_173385               4162 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus cartilage intermediate layer protein, nucleotide
            pyrophosphohydrolase (Cilp), transcript variant 1, mRNA.
ACCESSION   NM_173385
VERSION     NM_173385.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Zhang,Q.J., He,Y., Li,Y., Shen,H., Lin,L., Zhu,M., Wang,Z., Luo,X.,
            Hill,J.A., Cao,D., Luo,R.L., Zou,R., McAnally,J., Liao,J.,
            Bajona,P., Zang,Q.S., Yu,Y. and Liu,Z.P.
  TITLE     Matricellular Protein Cilp1 Promotes Myocardial Fibrosis in
            Response to Myocardial Infarction
  JOURNAL   Circ Res 129 (11), 1021-1035 (2021)
   PUBMED   34610755
  REMARK    GeneRIF: Matricellular Protein Cilp1 Promotes Myocardial Fibrosis
            in Response to Myocardial Infarction.
REFERENCE   3  (bases 1 to 4162)
  AUTHORS   Xiang,C., Frietze,K.K., Bi,Y., Li,Y., Dal Pozzo,V., Pal,S.,
            Alexander,N., Baubet,V., D'Acunto,V., Mason,C.E., Davuluri,R.V. and
            Dahmane,N.
  TITLE     RP58 Represses Transcriptional Programs Linked to Nonneuronal Cell
            Identity and Glioblastoma Subtypes in Developing Neurons
  JOURNAL   Mol Cell Biol 41 (7), e0052620 (2021)
   PUBMED   33903225
REFERENCE   4  (bases 1 to 4162)
  AUTHORS   Zhang,C.L., Zhao,Q., Liang,H., Qiao,X., Wang,J.Y., Wu,D., Wu,L.L.
            and Li,L.
  TITLE     Cartilage intermediate layer protein-1 alleviates pressure
            overload-induced cardiac fibrosis via interfering TGF-beta1
            signaling
  JOURNAL   J Mol Cell Cardiol 116, 135-144 (2018)
   PUBMED   29438665
  REMARK    GeneRIF: CILP-1 is a novel extracellular matrix protein possessing
            anti-fibrotic ability in pressure overload-induced fibrotic
            remodeling.
REFERENCE   5  (bases 1 to 4162)
  AUTHORS   Seki,S., Tsumaki,N., Motomura,H., Nogami,M., Kawaguchi,Y., Hori,T.,
            Suzuki,K., Yahara,Y., Higashimoto,M., Oya,T., Ikegawa,S. and
            Kimura,T.
  TITLE     Cartilage intermediate layer protein promotes lumbar disc
            degeneration
  JOURNAL   Biochem Biophys Res Commun 446 (4), 876-881 (2014)
   PUBMED   24631904
  REMARK    GeneRIF: Overexpression of CILP in the nucleus pulposus cells
            promotes disc degeneration, indicating that CILP plays a direct
            role in the pathogenesis of lumbar disc disease.
REFERENCE   6  (bases 1 to 4162)
  AUTHORS   Rolfe,R.A., Nowlan,N.C., Kenny,E.M., Cormican,P., Morris,D.W.,
            Prendergast,P.J., Kelly,D. and Murphy,P.
  TITLE     Identification of mechanosensitive genes during skeletal
            development: alteration of genes associated with cytoskeletal
            rearrangement and cell signalling pathways
  JOURNAL   BMC Genomics 15, 48 (2014)
   PUBMED   24443808
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 4162)
  AUTHORS   Bernardo,B.C., Belluoccio,D., Rowley,L., Little,C.B., Hansen,U. and
            Bateman,J.F.
  TITLE     Cartilage intermediate layer protein 2 (CILP-2) is expressed in
            articular and meniscal cartilage and down-regulated in experimental
            osteoarthritis
  JOURNAL   J Biol Chem 286 (43), 37758-37767 (2011)
   PUBMED   21880736
REFERENCE   8  (bases 1 to 4162)
  AUTHORS   Nakagawa,O., Arnold,M., Nakagawa,M., Hamada,H., Shelton,J.M.,
            Kusano,H., Harris,T.M., Childs,G., Campbell,K.P., Richardson,J.A.,
            Nishino,I. and Olson,E.N.
  TITLE     Centronuclear myopathy in mice lacking a novel muscle-specific
            protein kinase transcriptionally regulated by MEF2
  JOURNAL   Genes Dev 19 (17), 2066-2077 (2005)
   PUBMED   16140986
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK081544.1 and AC110235.13.
            
            On Jun 21, 2018 this sequence version replaced NM_173385.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK081544.1, AK036473.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4153              AK081544.1         2-4154
            4154-4162           AC110235.13        164082-164090
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 35.2 cM"
     gene            1..4162
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /note="cartilage intermediate layer protein, nucleotide
                     pyrophosphohydrolase"
                     /db_xref="GeneID:214425"
                     /db_xref="MGI:MGI:2444507"
     exon            1..44
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            45..82
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            83..233
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..3925
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; cartilage intermediate layer protein 1"
                     /codon_start=1
                     /product="cartilage intermediate layer protein 1 isoform 1
                     precursor"
                     /protein_id="NP_775561.1"
                     /db_xref="CCDS:CCDS23287.1"
                     /db_xref="GeneID:214425"
                     /db_xref="MGI:MGI:2444507"
                     /translation="MAAIKTWVFSFLVLEVTTVLGRQTMLAQSVRRVQPVKRTPKTLA
                     KPADSQESPGEWTTWFNIDHPGGQGDYERLDAIRFYYGERVCARPLRLEARTTDWMPA
                     GSTGQVVHGSPREGFWCLNREQRPGQNCSNYTVRFLCPPGSLRGDAEHIWSSWSPWSK
                     CSAACGHTGVQTRTRTCLAQTVSLCSEATEEGQLCMSQACTACDLTCPMGQVNADCDA
                     CMCQDFMLHGAISLPGGGPAPGAAVYLLAKAPKMLTRTDSSGRFRVPGLCPDGKTILK
                     ITKTKFAPIMITMPKTSLKSATINAEFVRAETPYIVMNPEMKARRAGQSVSLCCKATG
                     KPSPDKYFWYHNNTLLDPSLYKHESKLVLRNLQQDQAGEYFCKAQSDAGAVKSKVTQL
                     TVIAHDETPCNPTPESYLIRLPHDCFQNASNSFYYDVGRCPIKTCAGQQDNGIRCRDA
                     VENCCGISRTEEREIQCSGYTLPTKVAVECSCQRCAETRSIVRGRVTAADNGEPMRFG
                     HVYMGNNRVSMTGYKGTFTLHIPQDTERLVLTFVDRLQKFVNTTKVLPFNKKGSAVFH
                     EIKMLRQKEPITLEAMETNIIPLGEVIGEDPVAELEIPSKSFYRQNGEPFTGKVKASV
                     TFLDPRNISTATAAQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNAGKVKVHL
                     DSTQVKMPEHVPAMKLWSLNPDTGLWEEEGDFKFESQRRNKREERTFLVGNMEIRERR
                     LFNLDVPESRRCFIKVRTYRSERFLPSEQIQGVVVSVINLEPRTGFSSNPRAWGRFDS
                     VITGPNGACLPAFCDDQSPDAYSVYVLASLSGEELEAVESSPKFNPNAIGVPQPYLNK
                     LKYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAAHFRF
                     YQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRN
                     MGGTHRQTVGKLYGIRDVKSTRDRDQPNVSSACLEFKCSGMLYDQDRVDRTLVKVIPQ
                     GSCHRASVNSMLHEYLVNHLPLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPRTAK
                     EIALGRCFDGTSDGSSRIMKSNVGVALTFNCAERQVGRQSAFQYLQSTPARSPATGTV
                     QGRVPAMRQQRASRGGLRRRGSMGPSEIFWCCSTTSEQLSLVVLSPFCPSHVTAVVRL
                     MHRLSLVNLNISGLVQFVSLCLYLLSLPHDADCHRAPTMTKQRFSS"
     exon            234..326
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            327..596
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            597..776
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            777..1091
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1200
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1358
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1359..4162
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4134..4139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /note="hexamer: AATAAA"
     polyA_site      4157
                     /gene="Cilp"
                     /gene_synonym="9830114D16; C130036G17Rik; CILP-1"
                     /note="major polyA site"
ORIGIN      
        1 gaagaagtct tggaccccca gctaagagtc ttgctcagga cacgaaagaa gacatcttct
       61 gcacccgact tagtgattaa agatcattgg ctctatgaaa tatctctggg gaactacagg
      121 ttcaactttg tgaagcagcg tcacttatct gtgacacttc tacaagggag agatggcagc
      181 aatcaagact tgggtattct ctttcctggt ccttgaggtc accaccgtgc tggggagaca
      241 gacgatgctt gcccagtcag tgagaagagt ccagcctgtg aagaggaccc ccaagaccct
      301 tgccaagcct gccgactccc aggagagtcc cggagagtgg acaacatggt ttaacatcga
      361 ccacccaggt gggcagggtg actatgagcg gctggatgcc attcgcttct actatgggga
      421 gcgtgtgtgt gcccgtcccc tgcggctaga ggcccggacc actgactgga tgcctgcggg
      481 cagcactggc caggtggtcc acggcagtcc ccgtgagggc ttctggtgcc tcaacaggga
      541 gcagagacca ggccagaact gttccaatta taccgtgcgc ttcctctgcc caccagggtc
      601 cttgcgagga gatgcagagc acatctggag ttcctggtct ccctggagca agtgctcagc
      661 tgcctgcggt cacactgggg tgcagacccg tacccgcacc tgcttggcac agacagtgtc
      721 actgtgcagt gaggcgaccg aggagggcca gctctgcatg agccaggcct gcacagcttg
      781 tgacctgacc tgccccatgg gccaggtaaa tgctgactgt gatgcctgca tgtgccagga
      841 cttcatgctt cacggggcca tctccctccc tggaggtggc ccagctccag gagccgctgt
      901 ctacctgctg gccaaggcac caaagatgct gacccgaaca gacagcagcg ggaggttccg
      961 agttcctggc ttgtgtcctg atggcaaaac catcctgaaa atcaccaaga ccaagtttgc
     1021 cccaattatg atcacgatgc ccaagactag cctgaagtca gctaccatca atgcggagtt
     1081 tgtgagggca gagaccccat acattgtaat gaaccctgag atgaaagcac gtcgggctgg
     1141 gcagagtgtg tctctgtgct gcaaagccac ggggaaaccc agtccggaca agtacttctg
     1201 gtaccacaac aacacactgc tggatccctc cctctataag cacgagagca agctggtgct
     1261 gaggaacctg cagcaggacc aagcagggga gtacttctgt aaggctcaga gtgatgctgg
     1321 ggctgtgaag tccaaggtca cccagctcac tgtcattgca catgatgaga ctccttgcaa
     1381 cccaacccca gagagctacc ttatccggct gccccatgac tgctttcaga acgccagcaa
     1441 ttccttctac tacgacgtag gccgctgccc gatcaagacc tgtgcagggc agcaggacaa
     1501 tgggatccgg tgccgagatg ctgtggagaa ctgctgcggc atttccagaa cagaagagag
     1561 ggagatccag tgcagtgggt acacactgcc caccaaggtg gctgtggagt gcagctgcca
     1621 acgatgcgca gagacacgga gcatcgtccg gggccgagtc actgctgctg acaacgggga
     1681 gcccatgcgc tttggccatg tgtacatggg gaataaccgc gtgagcatga ctggctacaa
     1741 aggcaccttc acactccaca tcccccagga cacggagagg ctggtgctca catttgtgga
     1801 caggctgcag aagtttgtca acacaaccaa agtgctgccc ttcaacaaga agggaagcgc
     1861 agtgttccac gagatcaaga tgcttcggca gaaagagccc atcacgttgg aagccatgga
     1921 gaccaacatt atccccctgg gagaggtgat tggtgaagac cctgtggctg agctggagat
     1981 cccatccaaa agtttctata ggcagaacgg ggagcccttc acaggaaagg taaaggccag
     2041 cgtgaccttc ctggatcctc ggaacatctc gacggccaca gctgcccaaa gtgacctcaa
     2101 cttcatcaat gatgaaggag acacttttcc ccttagaaca tatggtatgt tttcagtgga
     2161 ctttagagat gaggccactt ccgagtcact taatgctggc aaggtgaagg tccaccttga
     2221 ctcgacccag gtcaaaatgc cagagcatgt gccagccatg aaactctggt cgctcaaccc
     2281 agacacagga ctgtgggaag aggaagggga tttcaagttt gaaagccaaa ggaggaacaa
     2341 gagggaagag agaaccttcc tagtgggcaa tatggagatc cgggagagaa ggctctttaa
     2401 cctggacgtc cctgaaagcc gaaggtgctt cattaaggtg aggacttacc ggagtgagag
     2461 gttcctgccc agtgagcaaa ttcagggagt tgtggtctca gtgatcaatc tggagcccag
     2521 aactggcttc tcatctaacc ccagggcctg gggccgattt gacagtgtca tcacagggcc
     2581 caatggggcc tgcctgcctg ccttctgtga tgatcagtcc cccgatgctt actctgtcta
     2641 tgtcttggca agcctttctg gagaagaact agaggcagtt gagtcttctc ctaaattcaa
     2701 cccaaatgca atcggtgtcc ctcagcctta cctcaacaaa cttaaatacc gtcggacaga
     2761 ccatgaggat ccacgggtta aaaagacggc tttccaaatc agtatggcca aaccaaggcc
     2821 caactcagct gaggagagca atgggcctat ctatgccttt gagaaccttc gggcgtgtga
     2881 ggaggcaccc cccagtgcag ctcacttccg gttctatcag atcgaggggg atcgctatga
     2941 ctataacaca gttcctttca atgaagatga ccctatgagc tggactgaag actacctggc
     3001 atggtggccc aagccaatgg agttcagggc ctgctatatc aaggttaaga ttgtggggcc
     3061 actggaggtg aatgtacgat cccgtaacat gggaggcacc caccggcaga ctgtggggaa
     3121 gctctatggg atccgggatg tgaagagtac tcgggacaga gaccaaccta atgtctcatc
     3181 tgcctgcctg gagttcaagt gcagtggaat gctgtatgac caagaccgtg tagaccgcac
     3241 attagtaaag gttatccccc agggcagctg ccatcgagct agtgttaact ctatgttgca
     3301 cgagtacctg gtcaaccacc ttcccctggc agtcaacaat gacaccagcg agtatactat
     3361 gctggcaccc ctggaccctc tgggccacaa ttacggtatc tatactgtta ctgatcagga
     3421 ccctcgtaca gctaaggaga ttgcgctcgg ccggtgcttt gatggcactt ccgatggctc
     3481 ctccagaatc atgaagagca atgtgggagt tgccctgacc tttaactgtg cagaaaggca
     3541 ggtaggccgc cagagtgcct tccagtacct ccaaagcaca ccggccaggt ccccagctac
     3601 aggcactgtc caaggaagag taccagccat gaggcaacaa cgggcaagca ggggtggcct
     3661 acgccggcgt ggaagcatgg gcccctctga gattttctgg tgttgctcaa caacctctga
     3721 gcaactaagt cttgtggttc ttagtccttt ctgtccatct catgtgacag ctgtcgtgag
     3781 actgatgcac agactgtcac ttgttaattt aaacatatct ggtttggtgc agtttgtttc
     3841 tttgtgcctt tacttactgt ctctgcctca tgatgctgat tgccacaggg cccccacgat
     3901 gacaaaacaa agattctcct cttaaaaaga aacaaaagga attggtaaac gtctgcagct
     3961 ttggctgaaa acacacatct tattgtatgg ttttgccagc ctttacacta atgataatct
     4021 aatactgagg ctcaagtgaa caacaataaa acatttttct tggccatgtt ccacaagatg
     4081 taagcaaagt ctctgtcaca gtccatatat atataaatgg tggtgaataa ggaaataaac
     4141 aatgttttta tgtgaaatgc aa
//
