LOCUS       NM_019987               6640 bp    mRNA    linear   ROD 28-AUG-2024
DEFINITION  Mus musculus ciliogenesis associated kinase 1 (Cilk1), transcript
            variant 2, mRNA.
ACCESSION   NM_019987
VERSION     NM_019987.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Limerick,A., McCabe,E.A., Turner,J.S., Kuang,K.W., Brautigan,D.L.,
            Hao,Y., Chu,C.Y., Fu,S.H., Ahmadi,S., Xu,W. and Fu,Z.
  TITLE     An Epilepsy-Associated CILK1 Variant Compromises KATNIP Regulation
            and Impairs Primary Cilia and Hedgehog Signaling
  JOURNAL   Cells 13 (15), 1258 (2024)
   PUBMED   39120290
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6640)
  AUTHORS   Rah,G., Cha,H., Kim,J., Song,J., Kim,H., Oh,Y.K., Ahn,C., Kang,M.,
            Kim,J., Yoo,K.H., Kim,M.J., Ko,H.W., Ko,J.Y. and Park,J.H.
  TITLE     KLC3 Regulates Ciliary Trafficking and Cyst Progression in CILK1
            Deficiency-Related Polycystic Kidney Disease
  JOURNAL   J Am Soc Nephrol 33 (9), 1726-1741 (2022)
   PUBMED   35961787
REFERENCE   4  (bases 1 to 6640)
  AUTHORS   Yang,Y., Paivinen,P., Xie,C., Krup,A.L., Makela,T.P., Mostov,K.E.
            and Reiter,J.F.
  TITLE     Ciliary Hedgehog signaling patterns the digestive system to
            generate mechanical forces driving elongation
  JOURNAL   Nat Commun 12 (1), 7186 (2021)
   PUBMED   34893605
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6640)
  AUTHORS   Salvati,K.A., Mason,A.J., Gailey,C.D., Wang,E.J., Fu,Z. and
            Beenhakker,M.P.
  TITLE     Mice Harboring a Non-Functional CILK1/ICK Allele Fail to Model the
            Epileptic Phenotype in Patients Carrying Variant CILK1/ICK
  JOURNAL   Int J Mol Sci 22 (16), 8875 (2021)
   PUBMED   34445580
  REMARK    GeneRIF: Mice Harboring a Non-Functional CILK1/ICK Allele Fail to
            Model the Epileptic Phenotype in Patients Carrying Variant
            CILK1/ICK.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6640)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   7  (bases 1 to 6640)
  AUTHORS   Chen,T., Wu,D., Moskaluk,C.A. and Fu,Z.
  TITLE     Distinct expression patterns of ICK/MAK/MOK protein kinases in the
            intestine implicate functional diversity
  JOURNAL   PLoS One 8 (11), e79359 (2013)
   PUBMED   24244486
  REMARK    GeneRIF: the expressions of ICK/MAK/MOK proteins in the intestinal
            tract can be differentially and dynamically regulated, implicating
            a significant functional diversity within this group of protein
            kinases.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6640)
  AUTHORS   Sturgill,T.W., Stoddard,P.B., Cohn,S.M. and Mayo,M.W.
  TITLE     The promoter for intestinal cell kinase is head-to-head with F-Box
            9 and contains functional sites for TCF7L2 and FOXA factors
  JOURNAL   Mol Cancer 9, 104 (2010)
   PUBMED   20459822
  REMARK    GeneRIF: ICK and FBX9 are divergently transcribed from a
            bidirectional promoter that is GC-rich and contains a CpG island.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 6640)
  AUTHORS   Togawa,K., Yan,Y.X., Inomoto,T., Slaugenhaupt,S. and Rustgi,A.K.
  TITLE     Intestinal cell kinase (ICK) localizes to the crypt region and
            requires a dual phosphorylation site found in map kinases
  JOURNAL   J Cell Physiol 183 (1), 129-139 (2000)
   PUBMED   10699974
REFERENCE   10 (bases 1 to 6640)
  AUTHORS   Mombaerts,P., Anderson,S.J., Perlmutter,R.M., Mak,T.W. and
            Tonegawa,S.
  TITLE     An activated lck transgene promotes thymocyte development in RAG-1
            mutant mice
  JOURNAL   Immunity 1 (4), 261-267 (1994)
   PUBMED   7889413
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159313.3, AK087484.1, AK173068.1 and BM935223.1.
            
            On Aug 6, 2009 this sequence version replaced NM_019987.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF225918.1, AK087484.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AC159313.3         170891-170970       c
            81-771              AK087484.1         4-694
            772-6637            AK173068.1         1-5866
            6638-6640           BM935223.1         32-34               c
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 43.56 cM"
     gene            1..6640
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /note="ciliogenesis associated kinase 1"
                     /db_xref="GeneID:56542"
                     /db_xref="MGI:MGI:1934157"
     exon            1..218
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            219..495
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     CDS             395..2284
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /EC_number="2.7.11.1"
                     /note="MAK-related kinase; mICK; intestinal cell kinase"
                     /codon_start=1
                     /product="serine/threonine-protein kinase ICK"
                     /protein_id="NP_064371.2"
                     /db_xref="CCDS:CCDS40697.1"
                     /db_xref="GeneID:56542"
                     /db_xref="MGI:MGI:1934157"
                     /translation="MNRYTTIKQLGDGTYGSVLLGRSIESGELIAIKKMKRKFYSWEE
                     CMNLREVKSLKKLNHANIVKLKEVIRENDHLYFIFEYMKENLYQLIKERNKLFPESAI
                     RNIMYQILQGLAFIHKHGFFHRDLKPENLLCMGPELVKIADFGLAREIRSRPPYTDYV
                     STRWYRAPEVLLRSTNYSSPIDIWAVGCIMAEVYTLRPLFPGASEIDTIFKICQVLGT
                     PKKTDWPEGYQLSSAMNFLWPQCIPNNLKTLIPNASSEAIQLLRDLLQWDPKKRPTAS
                     QALRYPYFQIGHPLGIISKDSGKPQREVQDKTGPPPYIKPAPPAQAPAKAYTLISSRP
                     SQASQPPQHSVHPYKGDVSRTEQLSHVQEGKPSPPFFPSLHNKNLQPKILASLEQKNG
                     EIKPKSRRRWGLISRSTKGSDDWADLDDLDFSPSLTRIDVKNKKRQSDDTLCRFESVL
                     DLKPSESVGTGTTVSTQASSQRRDTPTLQSSAKQHYLKHSRYLPGINIRNGVLPNPGK
                     DFLPSNSWSSSGLSGKSSGTVSVVSKITSVGSGSASSSGLTGSYIPSFLKKEIGSVMQ
                     RVQLAPLAAPPPGYSSLKAVRPHPGRPFFHTQPRSTPGLIPRPPAAQPVHGRIDWSSK
                     YPSRR"
     exon            496..550
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            551..672
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            673..752
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            753..885
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            886..1057
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1225
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1537
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1728
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1877
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..2006
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2129
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..6640
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2450..2455
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /note="hexamer: TATAAA"
     polyA_site      2472
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /note="major polyA site"
     regulatory      4966..4971
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /note="hexamer: AATAAA"
     polyA_site      4984
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
     regulatory      6620..6625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
                     /note="hexamer: AATAAA"
     polyA_site      6640
                     /gene="Cilk1"
                     /gene_synonym="2210420N10Rik; Ick; Mrk"
ORIGIN      
        1 tgcagggcag cttttccagg gaaagcggct ggtcgtcccc actagaagcc actctgcctt
       61 ttgtttgttt tgctgccgga ggccgcggag cggcgggttc ctgctggatg ccacgcgctg
      121 acgggtgtgg acttgccggg ttcgcccgct cggatcccac tgcccttgcc tgccgggccc
      181 tctcgggtgc acgcgccgcc gggccgcgat cgccgccagc agaagactga gctttgaaaa
      241 tatgtatttg ggagataatc acgttctgtt gaataccttg tgctggtgct gccatcgaaa
      301 aatctggtta cagtctgggg aggcctgcta ccattgcagg actgaaccgc ctcggccctg
      361 agatgagtgt cccgacagag caggcgcacg caccatgaat agatacacaa cgatcaagca
      421 gcttggggat gggacctacg gctctgtcct gctggggaga agcattgagt ctggagaact
      481 gattgccatt aaaaaaatga aaagaaagtt ttactcctgg gaagaatgca tgaaccttcg
      541 ggaggttaag tctttaaaga agcttaatca tgccaatatc gtaaagttaa aagaggttat
      601 cagggaaaac gaccatcttt actttatctt cgagtacatg aaggaaaacc tttaccagct
      661 cattaaagaa agaaataagt tgtttccaga gtctgctata aggaatataa tgtaccagat
      721 attgcaagga ctggcattca ttcacaaaca cggtttcttc caccgggact taaaacccga
      781 gaacctcctc tgcatgggac cagaacttgt gaaaatcgcc gactttggat tggctcgaga
      841 aatccgatca agacctccgt acacagacta tgtgtctact agatggtaca gggctccaga
      901 ggtactgctg agatccacca actacagctc ccccattgac atctgggcgg taggctgcat
      961 catggcggag gtgtacacgc tccggcccct tttccctggg gccagtgaaa ttgacacaat
     1021 tttcaaaatt tgccaagtgt tgggaacacc aaagaagact gactggcctg aaggctacca
     1081 gctgtcaagt gctatgaatt tcctctggcc ccagtgcata cccaataact taaagactct
     1141 aatcccaaat gctagcagcg aagccattca gctcctgaga gacttgcttc agtgggatcc
     1201 caagaaacga ccaaccgcta gtcaggctct tcgatatcct tacttccaga tcggacaccc
     1261 actgggcatc atcagcaagg actcaggaaa accacagaga gaggtccagg acaagacagg
     1321 ccctcctcct tacatcaagc cagcccctcc tgcccaggcc ccggccaagg catatacgct
     1381 gatttcttca cgaccaagcc aagccagtca gccccctcag cattctgtgc acccctacaa
     1441 aggcgatgtc tctaggactg agcagctgag ccatgttcag gagggcaagc caagccctcc
     1501 attcttccca tccctccaca ataagaatct tcagccaaaa atcctagcca gcctggaaca
     1561 gaaaaatggt gaaatcaagc caaagagcag gagaagatgg ggtctcatct ccaggtcgac
     1621 aaagggttct gacgactggg ccgacttgga tgacttagac ttcagtccct ctctcaccag
     1681 gatagatgta aaaaacaaga agagacagag tgatgacacc ctctgcaggt ttgaaagtgt
     1741 tctggacctg aagccatctg agtcagtggg tacaggaacc actgtctcca cgcaggcttc
     1801 ctcgcagagg agagacacgc cgaccctgca gtcctcagca aagcagcact acttgaagca
     1861 ctctcgctac ttgcctggaa taaatataag aaatggcgtg ctcccaaatc caggcaagga
     1921 ctttcttcca tcaaattcat ggtccagttc cggcttgtct ggaaaatctt caggaacagt
     1981 atcagtagtg agcaaaataa cttcagttgg ctcaggctct gcgagctcta gtggactgac
     2041 tggaagctac attccttcct ttctgaaaaa ggaaatcggt tctgtcatgc agagggttca
     2101 gctggcgccc ctcgcagccc ctccccccgg ctactcttcc ctcaaggccg tgagacctca
     2161 cccggggagg cctttctttc acacccagcc caggagcacg cccggcttga tcccccgacc
     2221 tccagctgct cagcctgtgc acggcaggat agactggtct tccaagtacc catcccggcg
     2281 gtgactgtcc cggccacggg ccgtgctcct cagctggctc cattgtaccc gcacaaccac
     2341 gaggcggtgt aaaacaaccc acacttacag ctgttctgaa ctaatacatt tcaatatttc
     2401 ttttaaattt tttttaaaac attgatttga atgcaatacc ctctttttgt ataaaattat
     2461 tttattctaa aaccgggtct cattattttc ttaaacaaca gcattttgta tatatggatt
     2521 atgttttagc attttataca gtcaactttg taatgaactt tttaaagatt aagtgatttt
     2581 ttgttcattg ttccatgtaa tattttatac agattttgaa atatgtaata atattgattc
     2641 agtattgtga cagggtgcag tttaagacag tgggatacaa gataatttac ttgatatatt
     2701 tgaattttat ggagtggccc attcggtact ctaggctctc ttagactggt cttcggaagg
     2761 tggtctgctc actgcttttc tgaacagctt gtattgattg ttctgggggt ggaagttgtt
     2821 ttgttgtttt gagacagggt cctatgtagc ccagactggc ctcccactca ctatgtcgct
     2881 gaggatagct ttgaacttgg gatcttcctg tgtctacctg cctgagtgct gggatcgcag
     2941 gtgtgtaagc catgcttggt tttaccctgg gctttgtaca agcctcccag ccctgatcct
     3001 atcttttata cgattgtctt tcatacaacg gttctgtcct aaactatcgc tttctttaga
     3061 cattttactt tatttttgcc cccaaaacaa agaacttttc ataactagtt ggtgaaattt
     3121 tattacactc acaaacacag ctgcaaaaaa tccagataaa cccaggcata atcattcgag
     3181 acagcacagt cctacttctt tgttgctttg tattggttga catctctgct ggcctctgcc
     3241 ttcaccagcc cacacctgtg ggtggggatg gggatgaagg ggctgagtag gacagccatg
     3301 ggcagacaga gagatggcag agttgtagtc acctgttgct ctggtgagct tttttttttt
     3361 tggttccctg gggcctacca gccaggacct acctacccac agaactgcca gccagcctcc
     3421 ctcaggcccc accgagccac agaggcaggg tggggagctt gcagcccatc gaaggtgtct
     3481 ccacctgcac ccctctagcc agagaggagg ctccctccca cccctgcggt ccatactttt
     3541 tttccatttt taaaaatacc caggctggag agatgactca gccattccag ttccaaggga
     3601 tccgacgccc tcttctggct tctgtgggca catgcactcg tgtatataat tgccactgac
     3661 agggacacat acatgtaaaa tctttataaa aatctaattg ttaaaaatcc cttaaaaggg
     3721 aagctttctt atgtcaaaag tacctttttt attattttta ttaggatttc tgattgcttt
     3781 aatgtaaaat tttcttaaca tatggtaata caatcaatta agcttatatc cctgccaaga
     3841 tgaaagacca aaaaagccaa gtatctttct atgtagccat ggctgtcctg gaactcactc
     3901 tgtagacgag actgacctcg aacttagaga tctgcctgcc tctgcctacc aagggctggg
     3961 actaaagacg cacgccacca cacccagtga cttttttttt tttaaactga gagtgaaggt
     4021 tgtattcagc tgcattttcc acacctgact aggagcagct cataaacgcc gcaggccaac
     4081 ttctcatgac gattttagga gtagggaagc tttggggggt ggggagggga tctaaactgt
     4141 aagacgtcac actcgccagg atgttatgtt cagactgtgc cacggctcag cccccatctt
     4201 gtgtggcgtg tcttgaggtt gggacctctg ttggacagag gctgtcccat tgagaggaat
     4261 gtttacagca gttctacaaa tagcaaagct cgtctggaga cagaagcata cagaggatcc
     4321 acttgcttcc atctctgtgt gacgcagcca cctcctctcc tggttacctt acaggcaaaa
     4381 ggaagagtca cgaggccagt agccaacctc aaccgatggc agagccactc gcatgctccc
     4441 agggcccaaa ggacgttttt gttactgttt ccaaatgcgg caaagcaaac catatgaaac
     4501 gttctttttt gtagataaaa atggtaaggt gaagtgaatt cctcatacgt tagtaaatga
     4561 ttgctttgat gtattaacag cgacttccaa agggctctgt ctgccctcaa atcagaaagc
     4621 agtttatact ttggagaatc tgaatccaaa gtacactcga tggagccctt ttgatgctga
     4681 ggggcgtgca gaacgcacat cacgggcaag ggcttgagag ggaatgtttc cctgttaaca
     4741 agctagcagc aaaaccttag gaaatgcaca tgcaagcaca acttgaaagc gttcgtttct
     4801 gtattatatt ctaaaccaac aaaatgtgcg tccggagatg agtcctttac tgtaaggtta
     4861 atatttattt tctcttttct gtattatgta taaaaagtaa atctcaaatc ctgctcggct
     4921 agctgccaag ctgcctgcaa gctgtagata ccattaagat acagaaataa atctcgaacc
     4981 cccatggagt ctctgtaatc tagccagtgt taactaaggt cattatgttc ttttttaaaa
     5041 ttgacaagat cccagctacc cagttgtttg acgccatgaa attgttttcc ttcatgttct
     5101 gtggacccta acttggcttc ccttccttga ctagcaaaca gcttcccttc tgaagttagc
     5161 catgaaagtt tacatgagag cggcagctgt gcagctgagc aaaccagctt actggaaaac
     5221 caaattctaa agaactagca agaaccgcag acgtacgttt ctctcatcca aatgggtaaa
     5281 ggggaaattg tatgcaagct atgtgctata gacagaaata gccctttgta aacagagata
     5341 gtcgaatttg cctatcgcat atattttacc tactatagaa ttatgaagtg aaaagcaaaa
     5401 tgctgacagg agtgaggggt tagatggcac ctagtcatga ctgtacctca ttttcaattg
     5461 caaagctttg gagggctaga gagtggtcca gaggttaaga gcaccagctg ctcctccaga
     5521 aggcccaggt tcgattccca gcaccaaaga gacagctcac aaccatttat agttccagtt
     5581 ccaaggactc tgccctcttc tgagggcata cagtgcacag atatgaatgc agacaaaaca
     5641 cccatagaga gaaagtttaa attatattgc atcaaaataa aggagattta ttattaaaag
     5701 caatatcaaa acagctttct ggcaagtcag agtggctcat tctgggcctg gtttgttggc
     5761 atggatatgc tgagatgccc agagtttgcc tcacagagac aagggaaact atgtttgcat
     5821 aaaagctgct gctattagaa ttgtttcctt taaggtaagt cctgttaatt aaggagtgta
     5881 gaaagctgat cccgaaacaa tgtatcacag ttcttgtcta aggaaaacat acaactaaaa
     5941 cagccatact gccacgttcc tgaattctta agacctgagt acaggaaggc aaaatgtgtc
     6001 aagtgaaggc tcctttccct ttcagttcca aggataaata ccgttggagc taatgggcac
     6061 catcttttcc tgtgaagtca catttccata acatcaagcc attgtcttgt gccattgggg
     6121 ggtgggaatg gtggggcagg tgacacaggt cactgtatga ccagaagcga taagtttcta
     6181 aggaaatatt tgacatactt attgctttat gttgttctaa gcagatgttt gttcaataaa
     6241 ctgggtaggt attgtttaca attgtcttta caatttccaa actaatgtac tgtatgtttg
     6301 tataatttat ttgtatggtc tgctcatgta aattatcaag acaaaacagg ggctcgtatc
     6361 atcctaaggg gtgttggttt ttccttaaac actgatatgt gacttgaaca ccagctggac
     6421 aaagccttcc cagctcccac atctcagtac agaatgcagt tctgttccac tgactcgatt
     6481 ccaaagcaac cttcgcacca ttagaaggca aagcacaggg ggtaaagagg gaccctcctg
     6541 aaatacaatg ttactttttg aatgatttat cctgagatct ttgaggatgt taatactata
     6601 gatggaaact tctgctccaa ataaagttaa attcaagaaa
//
