LOCUS       NM_026635               1253 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus cytosolic iron-sulfur assembly component 2A (Ciao2a),
            transcript variant 1, mRNA.
ACCESSION   NM_026635
VERSION     NM_026635.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Liu,Z., Wang,H., Zhang,Z., Ma,Y., Jing,Q., Zhang,S., Han,J.,
            Chen,J., Xiang,Y., Kou,Y., Wei,Y., Wang,L. and Wang,Y.
  TITLE     Fam96a is essential for the host control of Toxoplasma gondii
            infection by fine-tuning macrophage polarization via an
            iron-dependent mechanism
  JOURNAL   PLoS Negl Trop Dis 18 (5), e0012163 (2024)
   PUBMED   38713713
  REMARK    GeneRIF: Fam96a is essential for the host control of Toxoplasma
            gondii infection by fine-tuning macrophage polarization via an
            iron-dependent mechanism.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1253)
  AUTHORS   Liu,Z., Xu,S., Zhang,Z., Wang,H., Jing,Q., Zhang,S., Liu,M.,
            Han,J., Kou,Y., Wei,Y., Wang,L. and Wang,Y.
  TITLE     FAM96A is essential for maintaining organismal energy balance and
            adipose tissue homeostasis in mice
  JOURNAL   Free Radic Biol Med 192, 115-129 (2022)
   PUBMED   36150559
  REMARK    GeneRIF: FAM96A is essential for maintaining organismal energy
            balance and adipose tissue homeostasis in mice.
REFERENCE   4  (bases 1 to 1253)
  AUTHORS   Yin,A., Chen,W., Cao,L., Li,Q., Zhu,X. and Wang,L.
  TITLE     FAM96A knock-out promotes alternative macrophage polarization and
            protects mice against sepsis
  JOURNAL   Clin Exp Immunol 203 (3), 433-447 (2021)
   PUBMED   33232517
  REMARK    GeneRIF: FAM96A knock-out promotes alternative macrophage
            polarization and protects mice against sepsis.
REFERENCE   5  (bases 1 to 1253)
  AUTHORS   Yin,A., Luo,Y., Chen,W., He,M., Deng,J.H., Zhao,N., Cao,L. and
            Wang,L.
  TITLE     FAM96A Protects Mice From Dextran Sulfate Sodium (DSS)-Induced
            Colitis by Preventing Microbial Dysbiosis
  JOURNAL   Front Cell Infect Microbiol 9, 381 (2019)
   PUBMED   31803631
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1253)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1253)
  AUTHORS   Stehling,O., Mascarenhas,J., Vashisht,A.A., Sheftel,A.D.,
            Niggemeyer,B., Rosser,R., Pierik,A.J., Wohlschlegel,J.A. and
            Lill,R.
  TITLE     Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and
            maturation of different subsets of cytosolic-nuclear iron-sulfur
            proteins
  JOURNAL   Cell Metab 18 (2), 187-198 (2013)
   PUBMED   23891004
  REMARK    Erratum:[Cell Metab. 2018 Jan 9;27(1):263. doi:
            10.1016/j.cmet.2017.12.009.. PMID: 29320706]
REFERENCE   8  (bases 1 to 1253)
  AUTHORS   Chen,K.E., Richards,A.A., Ariffin,J.K., Ross,I.L., Sweet,M.J.,
            Kellie,S., Kobe,B. and Martin,J.L.
  TITLE     The mammalian DUF59 protein Fam96a forms two distinct types of
            domain-swapped dimer
  JOURNAL   Acta Crystallogr D Biol Crystallogr 68 (Pt 6), 637-648 (2012)
   PUBMED   22683786
  REMARK    GeneRIF: putative metal-binding site of bacterial DUF59 proteins is
            not conserved in Fam96a, but Fam96a interacts tightly in vitro with
            Ciao1, the cytosolic iron-assembly protein
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK077700.1 and BE655498.1.
            
            On Aug 3, 2005 this sequence version replaced NM_026635.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK077700.1, AK002690.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1240              AK077700.1         2-1241
            1241-1253           BE655498.1         401-413
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 35.73 cM"
     gene            1..1253
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /note="cytosolic iron-sulfur assembly component 2A"
                     /db_xref="GeneID:68250"
                     /db_xref="MGI:MGI:1915500"
     exon            1..360
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..719
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /note="isoform 1 is encoded by transcript variant 1; MIP18
                     family protein FAM96A; family with sequence similarity 96,
                     member A"
                     /codon_start=1
                     /product="cytosolic iron-sulfur assembly component 2A
                     isoform 1"
                     /protein_id="NP_080911.1"
                     /db_xref="CCDS:CCDS23302.1"
                     /db_xref="GeneID:68250"
                     /db_xref="MGI:MGI:1915500"
                     /translation="MERVSGLLSWTLSRVLWLSGFSEHGAAWQPRIMEEKALEVYDLI
                     RTIRDPEKPNTLEELEVVTESCVEVQEINEDDYLVIIKFTPTVPHCSLATLIGLCLRV
                     KLQRCLPFKHKLEIYISEGTHSTEEDINKQINDKERVAAAMENPNLREIVEQCVLEPD
                     "
     exon            361..525
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /inference="alignment:Splign:2.1.0"
     exon            526..575
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /inference="alignment:Splign:2.1.0"
     exon            576..621
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /inference="alignment:Splign:2.1.0"
     exon            622..1253
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1218..1223
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /note="hexamer: AATAAA"
     polyA_site      1242
                     /gene="Ciao2a"
                     /gene_synonym="5730536A07Rik; Fam96a"
                     /note="major polyA site"
ORIGIN      
        1 cgtaaggatt tttctgtgcc ttctaaatcg agcagagcat cttgggacat gtagtcttta
       61 cagctaattt ccctaggtcc ttcgcgttgc agttttgagt cccgcctatt ctctgctccc
      121 ctaaaccttt acacatgtct tggtttattt gaagaccagg aagttctctc tcgaggtcgg
      181 ctgcggaaag acggtggctc ggttgggaca gtcgccaggg atggcggaac gtgaggatgg
      241 agcgagtgtc tgggctgctt tcctggacgc tgagcagggt cctatggctc tcaggctttt
      301 ctgagcacgg agctgcctgg cagccccgga tcatggaaga gaaagcgcta gaagtttatg
      361 atttgattcg aactatccgg gacccagaaa agcccaatac tttagaagaa ctggaagtgg
      421 tcacagagag ttgtgtggaa gttcaagaaa taaatgaaga tgactatttg gttattatca
      481 agttcacacc aacagtacct cattgctctt tggcaactct tattgggctg tgcctaagag
      541 taaagcttca gcggtgtctg ccgttcaaac acaagttgga aatctacatt tctgaaggaa
      601 ctcactcgac agaagaagac atcaacaagc agataaatga caaagagcga gtggcagctg
      661 caatggagaa ccccaacctt cgggaaatcg tggaacagtg cgtcctcgag ccggactgac
      721 ccctccctaa gagccagtgg cttagaagtg ttggatctgc tagtttcaat cctttgtaag
      781 atgtagagga cacatgttga ctagataggt gatttgtacc tcagagcttt ttttgttttt
      841 tgttttttcg agacaaggtt tctctgtgtg gccctggctg tcctggaact tgttctgttg
      901 accaggctgg ccttgaactc agagatccac ctggctctgt ctcccaaatg ctgggattaa
      961 aggtgtacac caccactgcc tggccttcag agcatctttt aagaggattc tttctgagca
     1021 aatttcagtt atgaggtaga ttctaattgt tcaatgagta acattctcag gatttctaac
     1081 tcaagtgatc agacagaaaa tattttctag ttattatgtg taaaatgtta ctatttttct
     1141 gatgaccatt ctgatacaac tgcttttcat gtcaaatatc tactgtgccc aaatgtattt
     1201 gatttaaacc attctaaaat aaatatgaca cgattctttt aatggctcac ctt
//
