LOCUS       NM_007700               3500 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus conserved helix-loop-helix ubiquitous kinase (Chuk),
            transcript variant 1, mRNA.
ACCESSION   NM_007700
VERSION     NM_007700.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Huang,W., Zhang,H., Wang,L., Zhang,F., Ma,M., Chen,D., Wan,X.,
            Zhang,Y. and Cao,C.
  TITLE     Ulinastatin attenuates renal ischemia-reperfusion injury by
            inhibiting NLRP3 inflammasome-triggered pyroptosis
  JOURNAL   Int Immunopharmacol 143 (Pt 1), 113306 (2024)
   PUBMED   39405939
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Cully,M.D., Nolte,J.E., Patel,A., Vaughan,A.E. and May,M.J.
  TITLE     Loss of Lymphatic IKKalpha Disrupts Lung Immune Homeostasis, Drives
            BALT Formation, and Protects against Influenza
  JOURNAL   Immunohorizons 8 (7), 478-491 (2024)
   PUBMED   39007717
  REMARK    GeneRIF: Loss of Lymphatic IKKalpha Disrupts Lung Immune
            Homeostasis, Drives BALT Formation, and Protects against Influenza.
REFERENCE   3  (bases 1 to 3500)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3500)
  AUTHORS   Song,N., Xu,Y., Paust,H.J., Panzer,U., de Las Noriega,M.M., Guo,L.,
            Renne,T., Huang,J., Meng,X., Zhao,M. and Thaiss,F.
  TITLE     IKK1 aggravates ischemia-reperfusion kidney injury by promoting the
            differentiation of effector T cells
  JOURNAL   Cell Mol Life Sci 80 (5), 125 (2023)
   PUBMED   37074502
  REMARK    GeneRIF: IKK1 aggravates ischemia-reperfusion kidney injury by
            promoting the differentiation of effector T cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3500)
  AUTHORS   Spella,M., Ntaliarda,G., Skiadas,G., Lamort,A.S., Vreka,M.,
            Marazioti,A., Lilis,I., Bouloukou,E., Giotopoulou,G.A.,
            Pepe,M.A.A., Weiss,S.A.I., Petrera,A., Hauck,S.M., Koch,I.,
            Lindner,M., Hatz,R.A., Behr,J., Arendt,K.A.M., Giopanou,I.,
            Brunn,D., Savai,R., Jenne,D.E., de Chateau,M., Yull,F.E.,
            Blackwell,T.S. and Stathopoulos,G.T.
  TITLE     Non-Oncogene Addiction of KRAS-Mutant Cancers to IL-1beta via
            Versican and Mononuclear IKKbeta
  JOURNAL   Cancers (Basel) 15 (6), 1866 (2023)
   PUBMED   36980752
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3500)
  AUTHORS   Nakano,H., Shindo,M., Sakon,S., Nishinaka,S., Mihara,M., Yagita,H.
            and Okumura,K.
  TITLE     Differential regulation of IkappaB kinase alpha and beta by two
            upstream kinases, NF-kappaB-inducing kinase and mitogen-activated
            protein kinase/ERK kinase kinase-1
  JOURNAL   Proc Natl Acad Sci U S A 95 (7), 3537-3542 (1998)
   PUBMED   9520401
REFERENCE   7  (bases 1 to 3500)
  AUTHORS   Ghosh,S., May,M.J. and Kopp,E.B.
  TITLE     NF-kappa B and Rel proteins: evolutionarily conserved mediators of
            immune responses
  JOURNAL   Annu Rev Immunol 16, 225-260 (1998)
   PUBMED   9597130
  REMARK    Review article
REFERENCE   8  (bases 1 to 3500)
  AUTHORS   Verma,I.M., Stevenson,J.K., Schwarz,E.M., Van Antwerp,D. and
            Miyamoto,S.
  TITLE     Rel/NF-kappa B/I kappa B family: intimate tales of association and
            dissociation
  JOURNAL   Genes Dev 9 (22), 2723-2735 (1995)
   PUBMED   7590248
  REMARK    Review article
REFERENCE   9  (bases 1 to 3500)
  AUTHORS   Mock,B.A., Connelly,M.A., McBride,O.W., Kozak,C.A. and Marcu,K.B.
  TITLE     CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human
            chromosome 10 and mouse chromosome 19
  JOURNAL   Genomics 27 (2), 348-351 (1995)
   PUBMED   7558004
REFERENCE   10 (bases 1 to 3500)
  AUTHORS   Connelly,M.A. and Marcu,K.B.
  TITLE     CHUK, a new member of the helix-loop-helix and leucine zipper
            families of interacting proteins, contains a serine-threonine
            kinase catalytic domain
  JOURNAL   Cell Mol Biol Res 41 (6), 537-549 (1995)
   PUBMED   8777433
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK164236.1, AK150039.1, AK035326.1 and AC124693.4.
            
            On Jun 30, 2009 this sequence version replaced NM_007700.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U12473.1, AK035326.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AK164236.1         2-25
            25-2097             AK150039.1         4-2076
            2098-3496           AK035326.1         2055-3453
            3497-3500           AC124693.4         136703-136706       c
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 36.71 cM"
     gene            1..3500
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /note="conserved helix-loop-helix ubiquitous kinase"
                     /db_xref="GeneID:12675"
                     /db_xref="MGI:MGI:99484"
     exon            1..190
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..2323
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /EC_number="2.7.11.10"
                     /note="isoform 1 is encoded by transcript variant 1;
                     inhibitor of nuclear factor kappa-B kinase subunit alpha;
                     nuclear factor NF-kappa-B inhibitor kinase alpha; I kappa
                     B kinase 1; I-kappa-B kinase alpha; IkappaB kinase alpha"
                     /codon_start=1
                     /product="inhibitor of nuclear factor kappa-B kinase
                     subunit alpha isoform 1"
                     /protein_id="NP_031726.2"
                     /db_xref="CCDS:CCDS29843.1"
                     /db_xref="GeneID:12675"
                     /db_xref="MGI:MGI:99484"
                     /translation="MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIK
                     SCRLELSSKNRERWCHEIQIMKKLDHANVVKACDVPEELNFLINDVPLLAMEYCSGGD
                     LRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKTI
                     HKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGY
                     RPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNSLCSLIVEPMESWL
                     QLMLNWDPQQRGGPIDLTLKQPRCFALMDHILNLKIVHILNMTSAKIISFLLPCDESL
                     HSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKT
                     VYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA
                     AMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLDLERYSEQMTYGISSEKML
                     KAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAID
                     LYKQLKHRPPDHLYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPK
                     VEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGYSLEGTVTPPVSAW
                     LPPTLADREHPLTCVVTPQDGETLAQMIEENLNCLGHLSTIIREANEDQSSSLMSLDW
                     SWLAE"
     exon            191..285
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            286..400
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            401..470
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            471..559
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            560..649
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            650..774
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            775..882
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1018
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1213
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1316
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1440
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1592
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1654
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1764
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1814
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1815..1911
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2059
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2193
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2194..2293
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..3500
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3480..3485
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /note="hexamer: ATTAAA"
     polyA_site      3499
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /note="major polyA site"
ORIGIN      
        1 gagacggagg actccgcaag cctcggcggc ccggaaccgg ccttagaccg gcggcgttgc
       61 ctgaggcggc tggcgctccc gccccatgga gcggcccccg gggctgcggc cgggcgcggg
      121 cggcccctgg gagatgcggg aacggcttgg caccggcggt ttcgggaacg tcagtctgta
      181 ccagcaccgg gaacttgatc tcaaaatagc aattaagtct tgtcgtttag agctaagttc
      241 caaaaacaga gagcgatggt gccatgaaat ccagatcatg aaaaagttgg accatgcgaa
      301 tgttgtaaag gcctgtgatg tccctgagga attgaacttt ttaattaacg atgtgcctct
      361 tctggcaatg gagtactgtt ctggagggga cctccggaag ctactcaaca aaccagaaaa
      421 ttgttgtgga cttaaagaaa gccagatact ttctttactg agtgacatag gatctgggat
      481 ccgatatctg catgaaaaca aaattataca tcgagatcta aaacctgaaa atatagttct
      541 tcaagatgtt ggtgggaaga caatacataa aataattgat ttgggttatg ccaaagatgt
      601 tgatcaagga agtctctgta catcttttgt gggaacattg cagtatttgg ccccagagct
      661 ctttgaaaat aagccgtaca cagccactgt ggattattgg agctttggga ccatggtgtt
      721 tgaatgtatt gctggatata ggcctttttt gcatcatctg cagccattta cctggcatga
      781 gaagattaag aagaaagatc caaagtgtat atttgcatgt gaagagatga ctggagaagt
      841 tcggtttagt agccatttac ctcagccaaa cagcctttgt agtttaatag tagagccaat
      901 ggaaagctgg ctccaattga tgctgaattg ggacccacag cagagagggg gacctattga
      961 tcttactttg aagcagccaa gatgttttgc attaatggat cacattctca atttaaagat
     1021 agtgcacatc ctaaatatga cttctgcaaa aatcatttct tttctgttac catgtgatga
     1081 aagtcttcat tcactacagt ctcgaattga gcgtgaaaca ggaataaata caggttctca
     1141 ggagcttctg tcagagacag ggatttctct ggatcctcgg aaaccagcct ctcagtgtgt
     1201 tctagatgga gttagaggct gtgatagcta catggtttat ttgtttgata aaagtaagac
     1261 tgtatatgaa ggaccatttg catccagaag tttatctgat tgtgtaaatt atattgtaca
     1321 agacagcaaa atacaactgc caattataca gctgcggaaa gtatgggctg aagcagtgca
     1381 ctacgtatct gggctaaagg aagactacag caggctcttc cagggacaaa gagcagcaat
     1441 gttaagtctt cttagatata atgctaactt gacaaaaatg aagaatactt tgatctcagc
     1501 atcacagcaa ctcaaagcta aattggagtt ttttcgaaaa agcattcagc ttgacttgga
     1561 gagatatagt gagcagatga cttatgggat atcttcagaa aaaatgttaa aagcatggaa
     1621 agaaatggaa gaaaaggcca ttcactattc tgaggttggt gtcattggtt atcttgagga
     1681 tcaaattatg tctttgcaca ctgaaatcat ggagctgcag aagagcccct atggacgacg
     1741 ccagggagac ttgatggagt ctctggagca gcgtgccatt gatctctata agcagctaaa
     1801 gcacagacct cctgatcact tgtacagcga cagcacagag atggtgaaga tcatcgtgca
     1861 caccgtgcag agtcaggacc gtgttctcaa ggagctgttt ggtcacctga gcaagttgtt
     1921 gggctgcaag cagaagatta ttgatctact ccccaaggtg gaagtggccc tcagtaacat
     1981 caaagaagct gacaatactg tcatgtttat gcagggaaag aggcagaaag aaatttggca
     2041 cctccttaaa attgcctgta cacagagttc tgcccgctct cttgtaggat acagtctaga
     2101 aggcacagta acccctccag tatcagcatg gctgccccct acattagcag accgtgaaca
     2161 tcctctgaca tgtgtggtaa ctcctcaaga tggagagacg ttagcacaaa tgatagaaga
     2221 aaatctgaac tgtcttggcc atttaagtac tattattcgt gaagcaaatg aggaccagag
     2281 cagtagtttg atgagtcttg attggagttg gttagcagaa tgactcgaca ctcgtttact
     2341 gtcctggagc ctacgaagct gttttgtcat ttactccaaa gtcatcttta cttgctgaag
     2401 ccattcctca cttaccagtc cgtgaggaga tggctgtgat cggaaactac gagtgacttt
     2461 acaagcacag tagcttggtg ttttgtttgt ttctaataat tatgatctct gaacagatag
     2521 aattttatag caaattagtg aaattaatta ttctttttaa caccgcaact aatgagggag
     2581 atcattagtg acctgcttat cttataaaat tggaaaaata ctactactag tttagctgat
     2641 gaaaaagata atcttctaaa ggcctaaatt ttcggcataa ggcccaacat ggtattagta
     2701 tacaggaatg aaaaattcac ccagtgttca tttgaagtaa agttttatct atgggttttc
     2761 tgtggaagag actgctgaca agtaaaattg ctcttcctga agactaagcc cagcctcctt
     2821 gtgttgctct cagcaagtgt tcttcatggc atcacatgga gtcagatgaa tcccatcttt
     2881 aatcacacat ttaatagagt ccttttcctg tgtaaggggt tggacttttg tgcctttgat
     2941 atcagctgac cataatgaat tgtgttgtgt gctatatgta tatgtattta aggtgtacat
     3001 ttaataatat caaagagaag atgcctgtta atttataatg tatttgaagg ttgtattgtt
     3061 tttgcatttg taaaaatggg ttacttgttt aaacaatctt ttatgtcttg tcatacaaat
     3121 tccaaagggt ctgcattcct ttatctgtaa ttacagtctc agaatccaag ttctgaaaac
     3181 aaggtatcta ttctgatctg acactggatc tgcttatccc atttagtgtg aatattcatt
     3241 gatttatgtg tttgattatt gggatgtgct gccacaggct ctcttgaagg ttgatgtagt
     3301 gtggcgtatg cactgaatta cctttctaaa atctgaacag ttctcattct gaaacatcta
     3361 gacttaaggg tttcagataa aagactgcgg ttctctgcct tatgttaaat aacttagaag
     3421 atgttatttt gtttgaaaaa atgtgaaatg cttttatatt ctagtttttc actttgcata
     3481 ttaaatgatt ttaaaattat
//
