LOCUS       NP_031726                745 aa            linear   ROD 27-MAR-2025
DEFINITION  inhibitor of nuclear factor kappa-B kinase subunit alpha isoform 1
            [Mus musculus].
ACCESSION   NP_031726
VERSION     NP_031726.2
DBSOURCE    REFSEQ: accession NM_007700.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 745)
  AUTHORS   Huang,W., Zhang,H., Wang,L., Zhang,F., Ma,M., Chen,D., Wan,X.,
            Zhang,Y. and Cao,C.
  TITLE     Ulinastatin attenuates renal ischemia-reperfusion injury by
            inhibiting NLRP3 inflammasome-triggered pyroptosis
  JOURNAL   Int Immunopharmacol 143 (Pt 1), 113306 (2024)
   PUBMED   39405939
REFERENCE   2  (residues 1 to 745)
  AUTHORS   Cully,M.D., Nolte,J.E., Patel,A., Vaughan,A.E. and May,M.J.
  TITLE     Loss of Lymphatic IKKalpha Disrupts Lung Immune Homeostasis, Drives
            BALT Formation, and Protects against Influenza
  JOURNAL   Immunohorizons 8 (7), 478-491 (2024)
   PUBMED   39007717
  REMARK    GeneRIF: Loss of Lymphatic IKKalpha Disrupts Lung Immune
            Homeostasis, Drives BALT Formation, and Protects against Influenza.
REFERENCE   3  (residues 1 to 745)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (residues 1 to 745)
  AUTHORS   Song,N., Xu,Y., Paust,H.J., Panzer,U., de Las Noriega,M.M., Guo,L.,
            Renne,T., Huang,J., Meng,X., Zhao,M. and Thaiss,F.
  TITLE     IKK1 aggravates ischemia-reperfusion kidney injury by promoting the
            differentiation of effector T cells
  JOURNAL   Cell Mol Life Sci 80 (5), 125 (2023)
   PUBMED   37074502
  REMARK    GeneRIF: IKK1 aggravates ischemia-reperfusion kidney injury by
            promoting the differentiation of effector T cells.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 745)
  AUTHORS   Spella,M., Ntaliarda,G., Skiadas,G., Lamort,A.S., Vreka,M.,
            Marazioti,A., Lilis,I., Bouloukou,E., Giotopoulou,G.A.,
            Pepe,M.A.A., Weiss,S.A.I., Petrera,A., Hauck,S.M., Koch,I.,
            Lindner,M., Hatz,R.A., Behr,J., Arendt,K.A.M., Giopanou,I.,
            Brunn,D., Savai,R., Jenne,D.E., de Chateau,M., Yull,F.E.,
            Blackwell,T.S. and Stathopoulos,G.T.
  TITLE     Non-Oncogene Addiction of KRAS-Mutant Cancers to IL-1beta via
            Versican and Mononuclear IKKbeta
  JOURNAL   Cancers (Basel) 15 (6), 1866 (2023)
   PUBMED   36980752
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 745)
  AUTHORS   Nakano,H., Shindo,M., Sakon,S., Nishinaka,S., Mihara,M., Yagita,H.
            and Okumura,K.
  TITLE     Differential regulation of IkappaB kinase alpha and beta by two
            upstream kinases, NF-kappaB-inducing kinase and mitogen-activated
            protein kinase/ERK kinase kinase-1
  JOURNAL   Proc Natl Acad Sci U S A 95 (7), 3537-3542 (1998)
   PUBMED   9520401
REFERENCE   7  (residues 1 to 745)
  AUTHORS   Verma,I.M., Stevenson,J.K., Schwarz,E.M., Van Antwerp,D. and
            Miyamoto,S.
  TITLE     Rel/NF-kappa B/I kappa B family: intimate tales of association and
            dissociation
  JOURNAL   Genes Dev 9 (22), 2723-2735 (1995)
   PUBMED   7590248
  REMARK    Review article
REFERENCE   8  (residues 1 to 745)
  AUTHORS   Mock,B.A., Connelly,M.A., McBride,O.W., Kozak,C.A. and Marcu,K.B.
  TITLE     CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human
            chromosome 10 and mouse chromosome 19
  JOURNAL   Genomics 27 (2), 348-351 (1995)
   PUBMED   7558004
REFERENCE   9  (residues 1 to 745)
  AUTHORS   Connelly,M.A. and Marcu,K.B.
  TITLE     CHUK, a new member of the helix-loop-helix and leucine zipper
            families of interacting proteins, contains a serine-threonine
            kinase catalytic domain
  JOURNAL   Cell Mol Biol Res 41 (6), 537-549 (1995)
   PUBMED   8777433
REFERENCE   10 (residues 1 to 745)
  AUTHORS   Tebo,J.M., Chaoqun,W., Ohmori,Y. and Hamilton,T.A.
  TITLE     Murine inhibitory protein-kappa B alpha negatively regulates kappa
            B-dependent transcription in lipopolysaccharide-stimulated RAW
            264.7 macrophages
  JOURNAL   J Immunol 153 (10), 4713-4720 (1994)
   PUBMED   7963540
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK164236.1, AK150039.1, AK035326.1 and AC124693.4.
            
            On Jun 30, 2009 this sequence version replaced NP_031726.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U12473.1, AK035326.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 36.71 cM"
     Protein         1..745
                     /product="inhibitor of nuclear factor kappa-B kinase
                     subunit alpha isoform 1"
                     /EC_number="2.7.11.10"
                     /note="inhibitor of nuclear factor kappa-B kinase subunit
                     alpha; nuclear factor NF-kappa-B inhibitor kinase alpha; I
                     kappa B kinase 1; I-kappa-B kinase alpha; IkappaB kinase
                     alpha"
                     /calculated_mol_wt=84674
     Region          21..309
                     /region_name="STKc_IKK_alpha"
                     /note="Catalytic domain of the Serine/Threonine kinase,
                     Inhibitor of Nuclear Factor-KappaB Kinase (IKK) alpha;
                     cd14039"
                     /db_xref="CDD:270941"
     Site            order(21..25,29,42,44,74,95..98,102,104,144,146,148..149,
                     151,165,168,183..186)
                     /site_type="active"
                     /db_xref="CDD:270941"
     Site            order(21..25,29,42,44,74,95..98,102,144,146,148..149,151,
                     165)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270941"
     Site            23
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by PKB/AKT1 and SGK1.
                     /evidence=ECO:0000250|UniProtKB:O15111; propagated from
                     UniProtKB/Swiss-Prot (Q60680.1)"
     Site            order(25,102,104,144,146,148,168,183..186)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:270941"
     Site            order(108,110,114,117,218,223,251..252,254,260..263)
                     /site_type="other"
                     /note="putative SDD interface [polypeptide binding]"
                     /db_xref="CDD:270941"
     Site            164..186
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:270941"
     Site            176
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by MAP3K14.
                     /evidence=ECO:0000250|UniProtKB:O15111; propagated from
                     UniProtKB/Swiss-Prot (Q60680.1)"
     Site            180
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by SGK1.
                     /evidence=ECO:0000250|UniProtKB:O15111; propagated from
                     UniProtKB/Swiss-Prot (Q60680.1)"
     Region          311..387
                     /region_name="Ubl_IKKA_like"
                     /note="ubiquitin-like (Ubl) domain found in inhibitor of
                     nuclear factor kappa-B kinases, IKK-alpha and IKK-beta,
                     and similar proteins; cd17046"
                     /db_xref="CDD:340566"
     Region          386..659
                     /region_name="IKBKB_SDD"
                     /note="IQBAL scaffold dimerization domain; pfam18397"
                     /db_xref="CDD:465745"
     Region          455..476
                     /region_name="Leucine-zipper"
                     /note="propagated from UniProtKB/Swiss-Prot (Q60680.1)"
     Region          707..743
                     /region_name="IKKbetaNEMObind"
                     /note="I-kappa-kinase-beta NEMO binding domain; pfam12179"
                     /db_xref="CDD:463485"
     Region          738..743
                     /region_name="NEMO-binding"
                     /note="propagated from UniProtKB/Swiss-Prot (Q60680.1)"
     CDS             1..745
                     /gene="Chuk"
                     /gene_synonym="Chuk1; Fbx24; Fbxo24; IKBKA; IKK alpha;
                     IKK1; Ikka; NFKBIKA"
                     /coded_by="NM_007700.2:86..2323"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS29843.1"
                     /db_xref="GeneID:12675"
                     /db_xref="MGI:MGI:99484"
ORIGIN      
        1 merppglrpg aggpwemrer lgtggfgnvs lyqhreldlk iaikscrlel ssknrerwch
       61 eiqimkkldh anvvkacdvp eelnflindv pllameycsg gdlrkllnkp enccglkesq
      121 ilsllsdigs girylhenki ihrdlkpeni vlqdvggkti hkiidlgyak dvdqgslcts
      181 fvgtlqylap elfenkpyta tvdywsfgtm vfeciagyrp flhhlqpftw hekikkkdpk
      241 cifaceemtg evrfsshlpq pnslcslive pmeswlqlml nwdpqqrggp idltlkqprc
      301 falmdhilnl kivhilnmts akiisfllpc deslhslqsr ieretgintg sqellsetgi
      361 sldprkpasq cvldgvrgcd symvylfdks ktvyegpfas rslsdcvnyi vqdskiqlpi
      421 iqlrkvwaea vhyvsglked ysrlfqgqra amlsllryna nltkmkntli sasqqlkakl
      481 effrksiqld leryseqmty gissekmlka wkemeekaih ysevgvigyl edqimslhte
      541 imelqkspyg rrqgdlmesl eqraidlykq lkhrppdhly sdstemvkii vhtvqsqdrv
      601 lkelfghlsk llgckqkiid llpkvevals nikeadntvm fmqgkrqkei whllkiactq
      661 ssarslvgys legtvtppvs awlpptladr ehpltcvvtp qdgetlaqmi eenlnclghl
      721 stiireaned qssslmsldw swlae
//
