LOCUS       NM_001081328            3882 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus chondroitin sulfate synthase 3 (Chsy3), mRNA.
ACCESSION   NM_001081328 NM_178636 XM_894542
VERSION     NM_001081328.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Hu,B., Lv,X., Wei,L., Wang,Y., Zheng,G., Yang,C., Zang,F., Wang,J.,
            Li,J., Wu,X., Yue,Z., Xiao,Q., Shao,Z., Yuan,W., Li,J., Cao,P.,
            Xu,C. and Chen,H.
  TITLE     Sensory Nerve Maintains Intervertebral Disc Extracellular Matrix
            Homeostasis Via CGRP/CHSY1 Axis
  JOURNAL   Adv Sci (Weinh) 9 (30), e2202620 (2022)
   PUBMED   36047655
REFERENCE   3  (bases 1 to 3882)
  AUTHORS   Rios,J.J., Denton,K., Russell,J., Kozlitina,J., Ferreira,C.R.,
            Lewanda,A.F., Mayfield,J.E., Moresco,E., Ludwig,S., Tang,M., Li,X.,
            Lyon,S., Khanshour,A., Paria,N., Khalid,A., Li,Y., Xie,X.,
            Feng,J.Q., Xu,Q., Lu,Y., Hammer,R.E., Wise,C.A. and Beutler,B.
  TITLE     Germline Saturation Mutagenesis Induces Skeletal Phenotypes in Mice
  JOURNAL   J Bone Miner Res 36 (8), 1548-1565 (2021)
   PUBMED   33905568
REFERENCE   4  (bases 1 to 3882)
  AUTHORS   Xiang,C., Frietze,K.K., Bi,Y., Li,Y., Dal Pozzo,V., Pal,S.,
            Alexander,N., Baubet,V., D'Acunto,V., Mason,C.E., Davuluri,R.V. and
            Dahmane,N.
  TITLE     RP58 Represses Transcriptional Programs Linked to Nonneuronal Cell
            Identity and Glioblastoma Subtypes in Developing Neurons
  JOURNAL   Mol Cell Biol 41 (7), e0052620 (2021)
   PUBMED   33903225
REFERENCE   5  (bases 1 to 3882)
  AUTHORS   Uijtdewilligen,P.J.E., Versteeg,E.M., van de Westerlo,E.M.A., van
            der Vlag,J., Daamen,W.F. and van Kuppevelt,T.H.
  TITLE     Dynamic Expression of Genes Involved in
            Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
  JOURNAL   Biomed Res Int 2018, 9873471 (2018)
   PUBMED   30228991
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3882)
  AUTHORS   Shimbo,M., Suzuki,R., Fuseya,S., Sato,T., Kiyohara,K., Hagiwara,K.,
            Okada,R., Wakui,H., Tsunakawa,Y., Watanabe,H., Kimata,K.,
            Narimatsu,H., Kudo,T. and Takahashi,S.
  TITLE     Postnatal lethality and chondrodysplasia in mice lacking both
            chondroitin sulfate N-acetylgalactosaminyltransferase-1 and -2
  JOURNAL   PLoS One 12 (12), e0190333 (2017)
   PUBMED   29287114
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3882)
  AUTHORS   Izumikawa,T., Uyama,T., Okuura,Y., Sugahara,K. and Kitagawa,H.
  TITLE     Involvement of chondroitin sulfate synthase-3 (chondroitin
            synthase-2) in chondroitin polymerization through its interaction
            with chondroitin synthase-1 or chondroitin-polymerizing factor
  JOURNAL   Biochem J 403 (3), 545-552 (2007)
   PUBMED   17253960
REFERENCE   8  (bases 1 to 3882)
  AUTHORS   Sakai,K., Kimata,K., Sato,T., Gotoh,M., Narimatsu,H., Shinomiya,K.
            and Watanabe,H.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a
            critical role in chondroitin sulfate synthesis in cartilage
  JOURNAL   J Biol Chem 282 (6), 4152-4161 (2007)
   PUBMED   17145758
REFERENCE   9  (bases 1 to 3882)
  AUTHORS   Silbert,J.E. and Freilich,L.S.
  TITLE     Biosynthesis of chondroitin sulphate by a Golgi-apparatus-enriched
            preparation from cultures of mouse mastocytoma cells
  JOURNAL   Biochem J 190 (2), 307-313 (1980)
   PUBMED   6781479
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC102257.5 and
            AC127301.3.
            
            On or before Jun 6, 2008 this sequence version replaced
            NM_178636.2, XM_894542.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC151164.1, SRR1660817.157592.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1145              AC102257.5         125095-126239
            1146-1429           AC102257.5         129020-129303
            1430-3882           AC127301.3         77011-79463         c
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 33.93 cM"
     gene            1..3882
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
                     /note="chondroitin sulfate synthase 3"
                     /db_xref="GeneID:78923"
                     /db_xref="MGI:MGI:1926173"
     exon            1..1145
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             338..2992
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
                     /EC_number="2.4.1.175"
                     /EC_number="2.4.1.226"
                     /note="chSy-2; chondroitin synthase 2; carbohydrate
                     synthase 2; N-acetylgalactosaminyltransferase 3;
                     chondroitin glucuronyltransferase 3;
                     N-acetylgalactosaminyl-proteoglycan
                     3-beta-glucuronosyltransferase 3;
                     glucuronosyl-N-acetylgalactosaminyl-proteoglycan
                     4-beta-N-acetylgalactosaminyltransferase II"
                     /codon_start=1
                     /product="chondroitin sulfate synthase 3"
                     /protein_id="NP_001074797.1"
                     /db_xref="CCDS:CCDS37829.1"
                     /db_xref="GeneID:78923"
                     /db_xref="MGI:MGI:1926173"
                     /translation="MAVRSRRPWVSVALGLVLGFTAASWLIAPRVAELSEKRRRGSSL
                     CSYYGRSATGPRADAQQLLPQPQSRPRLEQSPPPASHELPGPQQPEAAPGGPSFRSSP
                     WQQPALLPQRRRGHTPEGATALPGAPAAKGEPEEEDGGAADPRKGGRPGSSHNGSGDG
                     GAAVPTSGPGDFLYVGVMTAQKYLGSRALAAQRTWARFIPGRVEFFSSQQSPSAALGQ
                     PPPPLPVIALPGVDDSYPPQKKSFMMIKYMHDHYLDKYEWFMRADDDVYIKGDKLEEF
                     LRSLNSSKPLYLGQTGLGNTEELGKLGLEPGENFCMGGPGMIFSREVLRRMVPHIGEC
                     LREMYTTHEDVEVGRCVRRFGGTQCVWSYEMQQLFHENYEHNRKGYIQDLHNSKIHAA
                     ITLHPNKRPAYQYRLHNYMLSRKISELRYRTIQLHRESALMSKLSNSEVSKEDQQLGR
                     TPSFNHFQPRERNEVMEWEFLTGKLLYSAAENQPPRQSINSILRSALDDTVLQVMEMI
                     NENAKSRGRLIDFKEIQYGYRRVDPMHGVEYILDLLLLYKRHKGRKLTVPVRRHAYLQ
                     QPFSKPFFREVEELDVNRLVESINSGTQSFSVISNSLKILSSLQEAKDIGGHNEKKVH
                     ILVPLVGRYDIFLRFMENFESTCLIPKQNVKLVIILFSRDAGQESIKHIELIQEYQSR
                     YPSAEMMLIPMKGEFSRGLGLEMASSQFDNDTLLLFCDVDLIFRGDFLQRCRDNTVQG
                     QQVYYPIIFSQYDPKVTHMRNPPTEGDFVFSKETGFWRDYGYGITCIYKSDLLGAGGF
                     DTSIQGWGLEDVDLYNKVILSGLRPFRSQEVGVVHIFHPVHCDPNLDPKQYKMCLGSK
                     ASTFASTMQLAELWLEKHLGVRDNRTLS"
     exon            1146..1429
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..3882
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3823..3828
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3861
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
                     /note="major polyA site"
     regulatory      3864..3869
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
                     /note="hexamer: GATAAA"
     polyA_site      3882
                     /gene="Chsy3"
                     /gene_synonym="4833446K15Rik"
ORIGIN      
        1 gtgacagggt ggcagccgag aagcggacgc gttgctgccg ccgctcctcc tcctgcagct
       61 cctctcgcgg ccgccgctgt cgccgcctcc gcctctgccg ctgccgaagt ttcactcccg
      121 accctcggcg ggagccccag cgccgagcag agctgccacc tcgcccggag ctgcagcgca
      181 gctcggcgtg cggacagcca gcccgcgtcg gcgggcaggc agcgttccac ggccccgggc
      241 taggggcgcg gagacccagc cggggcggca gccagcctag gggacccgcg acagcgccca
      301 caccgcccac tctgcctctg tgttccgagc cgctacgatg gccgtgcgct cccgccgccc
      361 atgggtgagc gtggcattgg ggttggtcct gggcttcacc gccgcgtcct ggctcatcgc
      421 cccccgggtg gccgagctga gcgagaagag gcgacgcggc tccagtcttt gctcctacta
      481 cggccgctcg gctaccgggc cccgcgcgga cgcgcagcag ctgctccccc aaccccagtc
      541 ccggccgcgg ctagagcagt cgccgccccc tgccagccac gagctccccg gtcctcagca
      601 gccggaggcg gcgcccggag gtcccagttt tcggagcagc ccctggcagc agccggctct
      661 gttgccgcag aggaggcgag gacacacgcc cgaaggtgcg acggcgcttc ccggcgctcc
      721 ggctgccaaa ggggaaccag aggaggagga tgggggcgcg gctgaccctc ggaagggtgg
      781 ccggccgggg agcagccaca acggcagcgg ggacgggggt gccgctgtcc cgacctccgg
      841 acccggggac ttcctgtacg tgggtgtgat gaccgcacag aagtacctgg gcagtcgcgc
      901 gctggccgcg cagcggacct gggcgcgctt catccctggc cgcgtggagt tcttttccag
      961 tcagcaatct cccagtgctg cgcttggcca gcccccgcca cctttgcctg tcatcgcgct
     1021 gccaggggtc gacgattcct accctcccca gaaaaagtcc ttcatgatga tcaagtacat
     1081 gcacgaccac tatctggaca agtatgagtg gttcatgcgc gccgacgacg atgtctacat
     1141 caaaggtgat aagttagaag aatttctaag atccctaaat agcagcaagc ctctctacct
     1201 gggacagacg ggcctgggca atactgaaga acttggaaag ctggggctgg agcccgggga
     1261 gaacttctgc atgggaggac ctggcatgat cttcagcaga gaggttctca ggcggatggt
     1321 gcctcatatc ggcgaatgcc tccgagagat gtacaccaca cacgaagacg tagaagtagg
     1381 aaggtgtgtt cgccgtttcg gcgggacgca gtgtgtctgg tcttatgaga tgcagcagct
     1441 gttccatgaa aactacgaac acaatcgcaa gggttacatc caagacctcc acaacagcaa
     1501 aatccacgca gccatcacgc tccatccgaa caaaaggccc gcgtaccagt acagacttca
     1561 taactacatg ctcagccgca agatctccga gctccgctac cgcaccatcc agctccaccg
     1621 ggagagcgct ctcatgagca agctcagcaa cagtgaagtg agcaaagagg accaacagct
     1681 gggaaggacg ccgtccttca accacttcca gcctcgggag agaaatgaag tcatggagtg
     1741 ggagttcctg acggggaagc tgctttactc agctgcagag aaccagcctc ctcgacagag
     1801 catcaacagc atcctaaggt cagctctgga tgacactgtc ctgcaggtga tggagatgat
     1861 caacgagaat gccaagagta ggggccgact catcgacttc aaggaaattc agtatggcta
     1921 ccgcagggtt gatcccatgc acggggttga gtacatattg gacctgctac tcctgtacaa
     1981 aaggcacaaa ggaaggaaac tgactgtgcc tgtgaggcgc catgcctatc ttcagcagcc
     2041 atttagcaag cctttcttca gagaggtgga agaactcgac gtcaaccgtc tggtggagag
     2101 tatcaacagc ggtacacagt cattctccgt tatatccaat tctctaaaaa ttctctcttc
     2161 tcttcaagag gccaaagaca taggagggca caatgaaaag aaagtacaca ttctcgttcc
     2221 actcgttgga aggtacgaca ttttcttgag attcatggaa aattttgaaa gtacgtgtct
     2281 tatcccaaag caaaatgtca agcttgtcat catccttttc agcagggatg ctggccaaga
     2341 gtccatcaag cacattgagc tgatacaaga atatcagagc aggtatccca gtgcagaaat
     2401 gatgctcatt cccatgaagg gagagttttc cagaggtctt ggtcttgaaa tggcttcttc
     2461 ccagtttgac aatgacacat tgctgctatt ttgtgatgtt gacttgattt tcagaggaga
     2521 cttcctccaa cgctgtcgag acaatacagt tcagggacaa caggtatatt accccatcat
     2581 ctttagccag tatgacccaa aggtcaccca tatgagaaat cctcccacag agggtgactt
     2641 tgtattctca aaggaaactg ggttttggag agactatggc tacggaatca catgcattta
     2701 caaaagcgat ctactgggtg caggtggatt tgatacctca atacaaggct ggggactgga
     2761 agatgtagat ctctataata aagtcatcct atctggctta cggcccttca gaagtcaaga
     2821 agtgggagtg gtgcatattt tccatcctgt tcattgtgat cctaacttgg accctaagca
     2881 gtataagatg tgcttaggat ccaaagcaag tacttttgcc tcaaccatgc aactggctga
     2941 actctggtta gaaaaacatt tgggtgtcag ggataatcga actctctcct gacagtccag
     3001 gcaatacata ttgcctttgg aaaggggagt ttacctcagt gttggctgtt gttattttta
     3061 ttttattatt gttattttat tattattatt attattgttg ttgttgttga tgttgttgtt
     3121 gttataattt tattttgttg tcctggtctt aaagttgtct tccaaagggt gtttgttgac
     3181 ctcaagcaag aagagtctgc agttcactga tatttcacag tcagaagttc tactgaagtc
     3241 aatacatgtc ttacttttat atgactttat ttgagattga gttaaatcat ggcaatcaaa
     3301 tgactttggg agctcataca tcacaagaga cagcttagaa gaatgttctc ttagggctta
     3361 aagatgcaat attgactttt cttttgtttg tcaacttcaa tgtgatataa atatttacca
     3421 gtgaaaggta ccacaaaagt gctttatgct tcccaatggg gaagggactc tgtgacagaa
     3481 acttgagttt tgtaatttat acgaggactt aaatatatag acaataattt tcttcatcgt
     3541 tagaattttt aaagtaaatg aacacctatg attgtatgtt tattttttaa gaaaaaaaat
     3601 tttaaaaact gaggagtcct gttccacaag caagcggtac tggctaccct ggtcttacaa
     3661 ttacaaactc ctcacaactg tctgctctaa atgcgaagga actttatttt ctcaaggaaa
     3721 tatgatttaa tgaaatattc atgtacattt tagaagcttt atgaaacaat gtccttcatt
     3781 tgctggcaag aagataaaat atgacagaac ctgtttattt ataataaaac acaggtataa
     3841 ccgtattctt tttcaaaaac attgataaag ctctgttgtt ta
//
