LOCUS       NM_175140               2397 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus carbohydrate sulfotransferase 8 (Chst8), mRNA.
ACCESSION   NM_175140
VERSION     NM_175140.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2397)
  AUTHORS   Li,J., Sun,L., Peng,X.L., Yu,X.M., Qi,S.J., Lu,Z.J., Han,J.J. and
            Shen,Q.
  TITLE     Integrative genomic analysis of early neurogenesis reveals a
            temporal genetic program for differentiation and specification of
            preplate and Cajal-Retzius neurons
  JOURNAL   PLoS Genet 17 (3), e1009355 (2021)
   PUBMED   33760820
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2397)
  AUTHORS   Uijtdewilligen,P.J.E., Versteeg,E.M., van de Westerlo,E.M.A., van
            der Vlag,J., Daamen,W.F. and van Kuppevelt,T.H.
  TITLE     Dynamic Expression of Genes Involved in
            Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
  JOURNAL   Biomed Res Int 2018, 9873471 (2018)
   PUBMED   30228991
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2397)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 2397)
  AUTHORS   Mi,Y., Fiete,D. and Baenziger,J.U.
  TITLE     Ablation of GalNAc-4-sulfotransferase-1 enhances reproduction by
            altering the carbohydrate structures of luteinizing hormone in mice
  JOURNAL   J Clin Invest 118 (5), 1815-1824 (2008)
   PUBMED   18431515
REFERENCE   7  (bases 1 to 2397)
  AUTHORS   Boregowda,R.K., Mi,Y., Bu,H. and Baenziger,J.U.
  TITLE     Differential expression and enzymatic properties of
            GalNAc-4-sulfotransferase-1 and GalNAc-4-sulfotransferase-2
  JOURNAL   Glycobiology 15 (12), 1349-1358 (2005)
   PUBMED   16079414
  REMARK    GeneRIF: analysis of enzymatic properties and expression of
            GalNAc-4-sulfotransferase-1 and GalNAc-4-sulfotransferase-2
REFERENCE   8  (bases 1 to 2397)
  AUTHORS   Okuda,T., Sawada,T., Nakano,H., Matsubara,K., Matsuda,Y., Fukuta,M.
            and Habuchi,O.
  TITLE     Mouse N-acetylgalactosamine 4-sulfotransferases-1 and -2. Molecular
            cloning, expression, chromosomal mapping and detection of their
            activity with GalNAcbeta1-4GlcNAcbeta1-octyl
  JOURNAL   J Biochem 134 (1), 111-120 (2003)
   PUBMED   12944377
REFERENCE   9  (bases 1 to 2397)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 2397)
  AUTHORS   Xia,G., Evers,M.R., Kang,H.G., Schachner,M. and Baenziger,J.U.
  TITLE     Molecular cloning and expression of the pituitary glycoprotein
            hormone N-acetylgalactosamine-4-O-sulfotransferase
  JOURNAL   J Biol Chem 275 (49), 38402-38409 (2000)
   PUBMED   10988300
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC123805.4, CF171795.1, BX649462.9 and AK051290.1.
            
            On Aug 6, 2009 this sequence version replaced NM_175140.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051124.1, AF308145.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-45                AC123805.4         125738-125782       c
            46-619              CF171795.1         1-574
            620-620             BX649462.9         145647-145647       c
            621-684             CF171795.1         576-639
            685-2397            AK051290.1         267-1979
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 20.53 cM"
     gene            1..2397
                     /gene="Chst8"
                     /gene_synonym="1500011J21Rik"
                     /note="carbohydrate sulfotransferase 8"
                     /db_xref="GeneID:68947"
                     /db_xref="MGI:MGI:1916197"
     exon            1..366
                     /gene="Chst8"
                     /gene_synonym="1500011J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            367..582
                     /gene="Chst8"
                     /gene_synonym="1500011J21Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             453..1706
                     /gene="Chst8"
                     /gene_synonym="1500011J21Rik"
                     /note="galNAc-4-ST1; galNAc-4-O-sulfotransferase 1;
                     N-acetylgalactosamine-4-O-sulfotransferase 1; GalNAc4ST-1;
                     carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase
                     8"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 8"
                     /protein_id="NP_780349.3"
                     /db_xref="CCDS:CCDS21142.1"
                     /db_xref="GeneID:68947"
                     /db_xref="MGI:MGI:1916197"
                     /translation="MTPRLGTMRLACMFSSILLFGAAGLLLFISLQDPIELSPQQVPG
                     IKFSIRPQQPQHDSHLRISTEKGTRDSPSGSPRGLQLQAPDQPRPHPKAAGSPLRLRQ
                     RRRRLLIKKMPAAGTNQGNNSSETFIQPRPRTMDSRWVSLHQTQQERKRVMREACAKY
                     RASSSRRAVTPRHVSRIFVEDRHRVLYCEVPKAGCSNWKRVLMVLAGLASSTADIQHN
                     TVHYGSALKRLDTFDRQGIVHRLSTYTKMLFVREPFERLVSAFRDKFEHPNSYYHPVF
                     GKAILARYRANASREALRTGSGVQFPEFVQYLLDVHRPVGMDIHWDHVSRLCSPCLID
                     YDFVGKFESMEDDANFFLRLIHAPGNLTFPRFKDRHSEEARTTSRITHQYFAQLSSLQ
                     RQRTYDFYYMDYLMFNYSKPFSDLY"
     exon            583..620
                     /gene="Chst8"
                     /gene_synonym="1500011J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            621..2397
                     /gene="Chst8"
                     /gene_synonym="1500011J21Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2082..2087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chst8"
                     /gene_synonym="1500011J21Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2106
                     /gene="Chst8"
                     /gene_synonym="1500011J21Rik"
                     /note="major polyA site"
ORIGIN      
        1 cagccttgcc ggaggcttcg ctcgcacttc gctgggagcc ttcccggcgc gtgagccgga
       61 tccggtggca ccgcggggaa gagacaggac cgggcggtgg cggcagagac agggggacgc
      121 acccggtgca gaagatccaa taggagcacg ccgccgcaac ctctcccgcg cgctccggtc
      181 gccgactcta cgccgatcgc ccactccccg caccttggac tacaccggga aaaagacgca
      241 gcctggcccg aactggggcg gagatccttc ttgcttccca agagctccgg agggaagatt
      301 ctcccgccgc cgagaacccc gccggactgg aggaacccgt ggcctggaga gcgctggctg
      361 tgccagaccc cagcctgatg gatgtctggt gtggataatg agggaagaac gtgcctttta
      421 cacccaagag gtgaccccgg agcgtgcccc ggatgacccc acgactcgga acgatgcggc
      481 tagcctgcat gttctcgtcc atcctgctgt ttggagctgc gggcctgctc ctcttcatca
      541 gcctccagga ccctatagag ctcagccccc agcaagttcc aggtataaag ttcagcatca
      601 ggccccagca accccagcat gatagccact tgaggatatc cacagaaaag ggcacacgag
      661 attcacccag cgggtcgcca agaggcctcc agctgcaagc gcctgaccaa cctcgacctc
      721 acccgaaggc agcgggatct cctttgcgcc tccggcagcg caggcggaga ctgctcatca
      781 aaaagatgcc agccgcaggg actaaccaag gcaacaactc gtccgaaacc tttatccagc
      841 cgagaccccg caccatggac agtcgttggg tcagcctgca ccagacccaa caggagcgca
      901 agcgtgtgat gcgagaagcc tgcgctaaat acagggccag cagcagccgc agagctgtca
      961 ctccccgcca cgtctcccgc atcttcgtgg aggaccgcca ccgtgtactg tactgtgaag
     1021 tacccaaggc aggctgctcc aactggaaga gggtgctcat ggtgctggca gggttagcct
     1081 catccacggc agatatccaa cacaacaccg tccactatgg cagcgccctt aagcgcctgg
     1141 atacttttga ccggcagggc atagtgcacc gcctcagtac ctacaccaag atgctctttg
     1201 tccgggaacc ctttgagcgg ctggtctctg ctttccgaga caagtttgag catcctaaca
     1261 gctactatca tcctgtcttt ggcaaggcta tcctggcccg gtaccgcgcc aacgcctcgc
     1321 gggaggcact gcggactggc tccggtgtgc agttccccga gttcgtccag tacctgttgg
     1381 atgtccaccg gcccgtgggc atggacatcc actgggacca tgttagccgg ctgtgcagcc
     1441 cctgcctcat cgactatgac tttgtgggca agttcgagag catggaagac gatgccaact
     1501 tcttcctgcg tctcatccat gcgcccggga acctgacttt cccgaggttc aaggacaggc
     1561 actccgagga ggcgcggacc acatcgagaa tcacccatca gtacttcgct cagctctcct
     1621 cgctgcagcg acagcgaacc tacgacttct actacatgga ttacctgatg ttcaactact
     1681 ccaaaccttt ctcggacctg tactgagggc ggggcctgct ggtcaggggc ggggtctgcc
     1741 ggtcatgccc actcacctgc gcataggcgg cctccgggga ctgagctctg aggatgtgag
     1801 gccttgtggc tgtggcccta gggtgggcca cagaggccca gacaatggac cttgaccctt
     1861 gtcccacacc catttcctca ttgggttggc tgagtttgag acggagcacg actcggatgg
     1921 atgctttaag aactcagctg agctatgctg agctatgctg tccagggaag cctgagaccc
     1981 agaagagggc cccagcgtcg agggatgtcc tacatcccct tatcctttgc cttgtaccaa
     2041 accacgtggt ttgctgcttt tctatgaccc agggtcatct gaataaagca catggttttc
     2101 agagcagtgg tgtctactcc aggctgtgct gtgagctcat aggcaccctg ccctatgcct
     2161 tggggagtct tagaaaaaaa atttttaatg tattttacta ttaataatac gtgaatatgt
     2221 gcacatgagt gcaggtgtct gtgaaggctg tatggggtca gattcccccc cccccccaaa
     2281 gctggagtga caggcagctg tcaacctccc tgtaggagct gaaccgctag agcaatctga
     2341 gctcttaacc ttaaccgctg agcctgcttt tgttttggtg aatcaggatc ttgctgt
//
