LOCUS       NM_019950               1935 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus carbohydrate sulfotransferase 5 (Chst5), mRNA.
ACCESSION   NM_019950
VERSION     NM_019950.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Tsutsumiuchi,M., Hoshino,H., Kogami,A., Tsutsumiuchi,T.,
            Yokoyama,O., Akama,T.O. and Kobayashi,M.
  TITLE     Preferential expression of sialyl 6'-sulfo
            N-acetyllactosamine-capped O-glycans on high endothelial venules in
            human peripheral lymph nodes
  JOURNAL   Lab Invest 99 (10), 1428-1441 (2019)
   PUBMED   31148596
REFERENCE   3  (bases 1 to 1935)
  AUTHORS   Narentuya, Takeda-Uchimura,Y., Foyez,T., Zhang,Z., Akama,T.O.,
            Yagi,H., Kato,K., Komatsu,Y., Kadomatsu,K. and Uchimura,K.
  TITLE     GlcNAc6ST3 is a keratan sulfate sulfotransferase for the
            protein-tyrosine phosphatase PTPRZ in the adult brain
  JOURNAL   Sci Rep 9 (1), 4387 (2019)
   PUBMED   30867513
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1935)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 1935)
  AUTHORS   Parfitt,G.J., Pinali,C., Akama,T.O., Young,R.D., Nishida,K.,
            Quantock,A.J. and Knupp,C.
  TITLE     Electron tomography reveals multiple self-association of
            chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null
            mouse corneas
  JOURNAL   J Struct Biol 174 (3), 536-541 (2011)
   PUBMED   21440637
  REMARK    GeneRIF: Data suggest that in Chst5-KO mice, multiple chondroitin
            sulphate/dermatan sulphate proteoglycans have aggregated laterally
            and, possibly, end-to-end, with orthogonal extensions protruding
            from the main electron-dense stained filament.
REFERENCE   6  (bases 1 to 1935)
  AUTHORS   Hayashida,Y., Akama,T.O., Beecher,N., Lewis,P., Young,R.D.,
            Meek,K.M., Kerr,B., Hughes,C.E., Caterson,B., Tanigami,A.,
            Nakayama,J., Fukada,M.N., Tano,Y., Nishida,K. and Quantock,A.J.
  TITLE     Matrix morphogenesis in cornea is mediated by the modification of
            keratan sulfate by GlcNAc 6-O-sulfotransferase
  JOURNAL   Proc Natl Acad Sci U S A 103 (36), 13333-13338 (2006)
   PUBMED   16938851
REFERENCE   7  (bases 1 to 1935)
  AUTHORS   Akama,T.O., Misra,A.K., Hindsgaul,O. and Fukuda,M.N.
  TITLE     Enzymatic synthesis in vitro of the disulfated disaccharide unit of
            corneal keratan sulfate
  JOURNAL   J Biol Chem 277 (45), 42505-42513 (2002)
   PUBMED   12218059
REFERENCE   8  (bases 1 to 1935)
  AUTHORS   Akama,T.O., Nakayama,J., Nishida,K., Hiraoka,N., Suzuki,M.,
            McAuliffe,J., Hindsgaul,O., Fukuda,M. and Fukuda,M.N.
  TITLE     Human corneal GlcNac 6-O-sulfotransferase and mouse intestinal
            GlcNac 6-O-sulfotransferase both produce keratan sulfate
  JOURNAL   J Biol Chem 276 (19), 16271-16278 (2001)
   PUBMED   11278593
REFERENCE   9  (bases 1 to 1935)
  AUTHORS   Hemmerich,S., Lee,J.K., Bhakta,S., Bistrup,A., Ruddle,N.R. and
            Rosen,S.D.
  TITLE     Chromosomal localization and genomic organization for the
            galactose/ N-acetylgalactosamine/N-acetylglucosamine
            6-O-sulfotransferase gene family
  JOURNAL   Glycobiology 11 (1), 75-87 (2001)
   PUBMED   11181564
REFERENCE   10 (bases 1 to 1935)
  AUTHORS   Lee,J.K., Bhakta,S., Rosen,S.D. and Hemmerich,S.
  TITLE     Cloning and characterization of a mammalian
            N-acetylglucosamine-6-sulfotransferase that is highly restricted to
            intestinal tissue
  JOURNAL   Biochem Biophys Res Commun 263 (2), 543-549 (1999)
   PUBMED   10491328
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF176840.1 and AC127315.4.
            
            On Jul 28, 2006 this sequence version replaced NM_019950.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BY711808.1, AI115260.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1328              AF176840.1         24-1351
            1329-1935           AC127315.4         42991-43597
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 58.11 cM"
     gene            1..1935
                     /gene="Chst5"
                     /gene_synonym="Gn6st-3; GST-4; I-GlcNAc-6-ST"
                     /note="carbohydrate sulfotransferase 5"
                     /db_xref="GeneID:56773"
                     /db_xref="MGI:MGI:1931825"
     exon            1..68
                     /gene="Chst5"
                     /gene_synonym="Gn6st-3; GST-4; I-GlcNAc-6-ST"
                     /inference="alignment:Splign:2.1.0"
     exon            69..1935
                     /gene="Chst5"
                     /gene_synonym="Gn6st-3; GST-4; I-GlcNAc-6-ST"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..1271
                     /gene="Chst5"
                     /gene_synonym="Gn6st-3; GST-4; I-GlcNAc-6-ST"
                     /note="GST4; mIGn6ST; glcNAc6ST-3; intestinal
                     GlcNAc-6-sulfotransferase; N-acetylglucosamine
                     6-O-sulfotransferase 3; intestinal
                     N-acetylglucosamine-6-O-sulfotransferase;
                     galactose/N-acetylglucosamine/N-acetylglucosamine
                     6-O-sulfotransferase 4; I-GlcNAc6ST; carbohydrate
                     (N-acetylglucosamine 6-O) sulfotransferase 5"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 5"
                     /protein_id="NP_064334.1"
                     /db_xref="CCDS:CCDS22682.1"
                     /db_xref="GeneID:56773"
                     /db_xref="MGI:MGI:1931825"
                     /translation="MRLPRFSSTVMLSLLMVQTGILVFLVSRQVPSSPAGLGERVHVL
                     VLSSWRSGSSFVGQLFSQHPDVFYLMEPAWHVWDTLSQGSAPALHMAVRDLIRSVFLC
                     DMDVFDAYLPWRRNISDLFQWAVSRALCSPPVCEAFARGNISSEEVCKPLCATRPFGL
                     AQEACSSYSHVVLKEVRFFNLQVLYPLLSDPALNLRIVHLVRDPRAVLRSREQTAKAL
                     ARDNGIVLGTNGTWVEADPRLRVVNEVCRSHVRIAEAALHKPPPFLQDRYRLVRYEDL
                     ARDPLTVIRELYAFTGLGLTPQLQTWIHNITHGSGPGARREAFKTTSRDALSVSQAWR
                     HTLPFAKIRRVQELCGGALQLLGYRSVHSELEQRDLSLDLLLPRGMDSFKWASSTEKQ
                     PES"
     regulatory      1908..1913
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chst5"
                     /gene_synonym="Gn6st-3; GST-4; I-GlcNAc-6-ST"
                     /note="hexamer: AATAAA"
     polyA_site      1935
                     /gene="Chst5"
                     /gene_synonym="Gn6st-3; GST-4; I-GlcNAc-6-ST"
                     /note="major polyA site"
ORIGIN      
        1 accaccgact gagcggctct ttgtgtgcgc cctgggtgcg cagcgcagaa gcgcagcggg
       61 cagcgcaggc cctagccaga ggtatgcggc taccccgttt ctccagcact gtcatgcttt
      121 cgctcctgat ggtacagact ggcatcctgg tcttcctggt ctcccggcaa gtgccatcgt
      181 ccccagcagg ccttggggag cgtgtgcacg tgctggtact gtcctcgtgg cgctcgggct
      241 cgtccttcgt gggccagctc ttcagccaac accccgatgt cttctacctg atggagccgg
      301 cttggcacgt ctgggatacg ttgtcgcagg gcagtgcccc cgcactccac atggccgtgc
      361 gtgacctgat ccgctcagtg ttcctatgcg acatggacgt atttgatgcc tacctgccct
      421 ggcgccgcaa catctcggat ctcttccagt gggcggtgag ccgcgcattg tgctcacctc
      481 cggtctgcga agccttcgct cgtggcaaca tcagcagcga ggaggtgtgt aagcctctgt
      541 gcgcaacgcg gcccttcggc ctggctcagg aagcctgcag ctcctatagt cacgtcgtgc
      601 tcaaggaggt gcgcttcttt aacctacagg tgctctaccc gctgctcagc gaccctgcgc
      661 tcaacctgcg catcgtgcac ctagtgcgcg acccgcgggc cgtgctgcgc tcccgagagc
      721 agacagccaa ggcgctggca cgggacaatg gcatcgtcct gggtaccaac ggcacgtggg
      781 tggaggcgga cccccggctg cgcgtggtca acgaggtatg ccgcagccat gtgcgcatcg
      841 cagaggcagc cttgcacaag ccgccgccct tcttgcaaga tcgctaccgc ctggtgcgct
      901 acgaggatct ggcccgggac ccactcaccg taatccgtga actctatgcc ttcaccggcc
      961 tgggtctcac gccgcagctc cagacttgga tccacaatat cacgcatggt tcagggccag
     1021 gcgcgcgccg tgaagccttc aagaccacat ccagggatgc gctcagtgta tcccaggcct
     1081 ggcgccacac gctgcccttt gccaagattc gccgggtcca ggaactgtgc gggggtgcac
     1141 tgcagctgct gggttaccgg tctgtgcatt cggagcttga gcaaagggac ctctctctgg
     1201 acctcctgct gccaagaggc atggacagtt tcaagtgggc atcgtccacg gagaagcaac
     1261 cggaatctta gaattttagt ggagagaccc agctataaca ttagggtcta ttggagtatg
     1321 ataaagaaag ggcttggaga acccaaaagc aagtagctgg gagtgtgagt gatcttgtcc
     1381 tgtactagga aaggatggag tccaaatccc acatctcttt ctgtccagat tgtagttttc
     1441 ggttgggtct tttaggattt ggattcccac caagtactat cgaatggaaa gcaaaagctg
     1501 tgcccacttc cttcagagag gcagccagcc tcctactaaa gcacttcctt tctcgttgac
     1561 tctctcccct ctttgatcat accatgcaat cgcagagaat ggggtcccag gcctgctctg
     1621 gagtgcggga aaggcgcggc tgtgggctgg ctcctaaaat ctgtgcacct gcctctcgtt
     1681 ggctcaccca gacctctgct cactgccacg ccctagtatc tcagtccatc atagacttgg
     1741 acagttatgg gcctggtcaa ggaggaaaat gagacgatgc ttccctctgt gattctctgc
     1801 ctgaccttct agaagggaat ccaggcacac acacaaccat acctgaggag gatggctttt
     1861 taatgaatct ttgatttgtc ctgagatgaa agatcctaat ttatggaaat aaacataaat
     1921 atgctgcgtg atcca
//
