LOCUS       NP_064334                395 aa            linear   ROD 09-FEB-2025
DEFINITION  carbohydrate sulfotransferase 5 [Mus musculus].
ACCESSION   NP_064334
VERSION     NP_064334.1
DBSOURCE    REFSEQ: accession NM_019950.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 395)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 395)
  AUTHORS   Tsutsumiuchi,M., Hoshino,H., Kogami,A., Tsutsumiuchi,T.,
            Yokoyama,O., Akama,T.O. and Kobayashi,M.
  TITLE     Preferential expression of sialyl 6'-sulfo
            N-acetyllactosamine-capped O-glycans on high endothelial venules in
            human peripheral lymph nodes
  JOURNAL   Lab Invest 99 (10), 1428-1441 (2019)
   PUBMED   31148596
REFERENCE   3  (residues 1 to 395)
  AUTHORS   Narentuya, Takeda-Uchimura,Y., Foyez,T., Zhang,Z., Akama,T.O.,
            Yagi,H., Kato,K., Komatsu,Y., Kadomatsu,K. and Uchimura,K.
  TITLE     GlcNAc6ST3 is a keratan sulfate sulfotransferase for the
            protein-tyrosine phosphatase PTPRZ in the adult brain
  JOURNAL   Sci Rep 9 (1), 4387 (2019)
   PUBMED   30867513
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 395)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (residues 1 to 395)
  AUTHORS   Parfitt,G.J., Pinali,C., Akama,T.O., Young,R.D., Nishida,K.,
            Quantock,A.J. and Knupp,C.
  TITLE     Electron tomography reveals multiple self-association of
            chondroitin sulphate/dermatan sulphate proteoglycans in Chst5-null
            mouse corneas
  JOURNAL   J Struct Biol 174 (3), 536-541 (2011)
   PUBMED   21440637
  REMARK    GeneRIF: Data suggest that in Chst5-KO mice, multiple chondroitin
            sulphate/dermatan sulphate proteoglycans have aggregated laterally
            and, possibly, end-to-end, with orthogonal extensions protruding
            from the main electron-dense stained filament.
REFERENCE   6  (residues 1 to 395)
  AUTHORS   Hayashida,Y., Akama,T.O., Beecher,N., Lewis,P., Young,R.D.,
            Meek,K.M., Kerr,B., Hughes,C.E., Caterson,B., Tanigami,A.,
            Nakayama,J., Fukada,M.N., Tano,Y., Nishida,K. and Quantock,A.J.
  TITLE     Matrix morphogenesis in cornea is mediated by the modification of
            keratan sulfate by GlcNAc 6-O-sulfotransferase
  JOURNAL   Proc Natl Acad Sci U S A 103 (36), 13333-13338 (2006)
   PUBMED   16938851
REFERENCE   7  (residues 1 to 395)
  AUTHORS   Akama,T.O., Misra,A.K., Hindsgaul,O. and Fukuda,M.N.
  TITLE     Enzymatic synthesis in vitro of the disulfated disaccharide unit of
            corneal keratan sulfate
  JOURNAL   J Biol Chem 277 (45), 42505-42513 (2002)
   PUBMED   12218059
REFERENCE   8  (residues 1 to 395)
  AUTHORS   Akama,T.O., Nakayama,J., Nishida,K., Hiraoka,N., Suzuki,M.,
            McAuliffe,J., Hindsgaul,O., Fukuda,M. and Fukuda,M.N.
  TITLE     Human corneal GlcNac 6-O-sulfotransferase and mouse intestinal
            GlcNac 6-O-sulfotransferase both produce keratan sulfate
  JOURNAL   J Biol Chem 276 (19), 16271-16278 (2001)
   PUBMED   11278593
REFERENCE   9  (residues 1 to 395)
  AUTHORS   Hemmerich,S., Lee,J.K., Bhakta,S., Bistrup,A., Ruddle,N.R. and
            Rosen,S.D.
  TITLE     Chromosomal localization and genomic organization for the
            galactose/ N-acetylgalactosamine/N-acetylglucosamine
            6-O-sulfotransferase gene family
  JOURNAL   Glycobiology 11 (1), 75-87 (2001)
   PUBMED   11181564
REFERENCE   10 (residues 1 to 395)
  AUTHORS   Lee,J.K., Bhakta,S., Rosen,S.D. and Hemmerich,S.
  TITLE     Cloning and characterization of a mammalian
            N-acetylglucosamine-6-sulfotransferase that is highly restricted to
            intestinal tissue
  JOURNAL   Biochem Biophys Res Commun 263 (2), 543-549 (1999)
   PUBMED   10491328
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF176840.1 and AC127315.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BY711808.1, AI115260.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 58.11 cM"
     Protein         1..395
                     /product="carbohydrate sulfotransferase 5"
                     /note="GST4; mIGn6ST; glcNAc6ST-3; intestinal
                     GlcNAc-6-sulfotransferase; N-acetylglucosamine
                     6-O-sulfotransferase 3; intestinal
                     N-acetylglucosamine-6-O-sulfotransferase;
                     galactose/N-acetylglucosamine/N-acetylglucosamine
                     6-O-sulfotransferase 4; I-GlcNAc6ST; carbohydrate
                     (N-acetylglucosamine 6-O) sulfotransferase 5"
                     /calculated_mol_wt=44406
     Site            8..26
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QUP4.1)"
     Site            116
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9QUP4.1)"
     Site            142
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9QUP4.1)"
     Region          159..357
                     /region_name="Sulfotransfer_1"
                     /note="Sulfotransferase domain; cl21551"
                     /db_xref="CDD:473908"
     Site            229
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9QUP4.1)"
     Site            305
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9QUP4.1)"
     CDS             1..395
                     /gene="Chst5"
                     /gene_synonym="Gn6st-3; GST-4; I-GlcNAc-6-ST"
                     /coded_by="NM_019950.2:84..1271"
                     /db_xref="CCDS:CCDS22682.1"
                     /db_xref="GeneID:56773"
                     /db_xref="MGI:MGI:1931825"
ORIGIN      
        1 mrlprfsstv mlsllmvqtg ilvflvsrqv psspaglger vhvlvlsswr sgssfvgqlf
       61 sqhpdvfylm epawhvwdtl sqgsapalhm avrdlirsvf lcdmdvfday lpwrrnisdl
      121 fqwavsralc sppvceafar gnisseevck plcatrpfgl aqeacssysh vvlkevrffn
      181 lqvlypllsd palnlrivhl vrdpravlrs reqtakalar dngivlgtng twveadprlr
      241 vvnevcrshv riaeaalhkp ppflqdryrl vryedlardp ltvirelyaf tglgltpqlq
      301 twihnithgs gpgarreafk ttsrdalsvs qawrhtlpfa kirrvqelcg galqllgyrs
      361 vhseleqrdl sldlllprgm dsfkwasste kqpes
//
