LOCUS       NM_016803               6176 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus carbohydrate sulfotransferase 3 (Chst3), transcript
            variant 1, mRNA.
ACCESSION   NM_016803
VERSION     NM_016803.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Hu,B., Lv,X., Wei,L., Wang,Y., Zheng,G., Yang,C., Zang,F., Wang,J.,
            Li,J., Wu,X., Yue,Z., Xiao,Q., Shao,Z., Yuan,W., Li,J., Cao,P.,
            Xu,C. and Chen,H.
  TITLE     Sensory Nerve Maintains Intervertebral Disc Extracellular Matrix
            Homeostasis Via CGRP/CHSY1 Axis
  JOURNAL   Adv Sci (Weinh) 9 (30), e2202620 (2022)
   PUBMED   36047655
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Schaberg,E., Theocharidis,U., May,M., Lessmann,K., Schroeder,T. and
            Faissner,A.
  TITLE     Sulfation of Glycosaminoglycans Modulates the Cell Cycle of
            Embryonic Mouse Spinal Cord Neural Stem Cells
  JOURNAL   Front Cell Dev Biol 9, 643060 (2021)
   PUBMED   34169071
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6176)
  AUTHORS   Bachvarova,V., Dierker,T., Esko,J., Hoffmann,D., Kjellen,L. and
            Vortkamp,A.
  TITLE     Chondrocytes respond to an altered heparan sulfate composition with
            distinct changes of heparan sulfate structure and increased levels
            of chondroitin sulfate
  JOURNAL   Matrix Biol 93, 43-59 (2020)
   PUBMED   32201365
REFERENCE   4  (bases 1 to 6176)
  AUTHORS   Uijtdewilligen,P.J.E., Versteeg,E.M., van de Westerlo,E.M.A., van
            der Vlag,J., Daamen,W.F. and van Kuppevelt,T.H.
  TITLE     Dynamic Expression of Genes Involved in
            Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
  JOURNAL   Biomed Res Int 2018, 9873471 (2018)
   PUBMED   30228991
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6176)
  AUTHORS   Kai,Y., Tomoda,K., Yoneyama,H., Yoshikawa,M. and Kimura,H.
  TITLE     RNA interference targeting carbohydrate sulfotransferase 3
            diminishes macrophage accumulation, inhibits MMP-9 expression and
            promotes lung recovery in murine pulmonary emphysema
  JOURNAL   Respir Res 16, 146 (2015)
   PUBMED   26646821
  REMARK    GeneRIF: CHST3 siRNA diminishes accumulation of excessive
            macrophages and the mediators, leading to accelerate the functional
            recovery from airway damage by repair of the elastin network
            associated with pulmonary emphysema
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6176)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
REFERENCE   7  (bases 1 to 6176)
  AUTHORS   Uchimura,K., Kadomatsu,K., Nishimura,H., Muramatsu,H., Nakamura,E.,
            Kurosawa,N., Habuchi,O., El-Fasakhany,F.M., Yoshikai,Y. and
            Muramatsu,T.
  TITLE     Functional analysis of the chondroitin 6-sulfotransferase gene in
            relation to lymphocyte subpopulations, brain development, and
            oversulfated chondroitin sulfates
  JOURNAL   J Biol Chem 277 (2), 1443-1450 (2002)
   PUBMED   11696535
REFERENCE   8  (bases 1 to 6176)
  AUTHORS   Hemmerich,S., Lee,J.K., Bhakta,S., Bistrup,A., Ruddle,N.R. and
            Rosen,S.D.
  TITLE     Chromosomal localization and genomic organization for the
            galactose/ N-acetylgalactosamine/N-acetylglucosamine
            6-O-sulfotransferase gene family
  JOURNAL   Glycobiology 11 (1), 75-87 (2001)
   PUBMED   11181564
REFERENCE   9  (bases 1 to 6176)
  AUTHORS   Uchimura,K., Kadomatsu,K., Fan,Q.W., Muramatsu,H., Kurosawa,N.,
            Kaname,T., Yamamura,K., Fukuta,M., Habuchi,O. and Muramatsu,T.
  TITLE     Mouse chondroitin 6-sulfotransferase: molecular cloning,
            characterization and chromosomal mapping
  JOURNAL   Glycobiology 8 (5), 489-496 (1998)
   PUBMED   9597547
REFERENCE   10 (bases 1 to 6176)
  AUTHORS   Silbert,J.E. and Freilich,L.S.
  TITLE     Biosynthesis of chondroitin sulphate by a Golgi-apparatus-enriched
            preparation from cultures of mouse mastocytoma cells
  JOURNAL   Biochem J 190 (2), 307-313 (1980)
   PUBMED   6781479
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155640.5.
            
            On May 24, 2022 this sequence version replaced NM_016803.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219380.53855.1,
                                           ERR3765653.3276718.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-420               AC155640.5         45596-46015
            421-573             AC155640.5         70674-70826
            574-819             AC155640.5         76601-76846
            820-6176            AC155640.5         78036-83392
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 29.7 cM"
     gene            1..6176
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /note="carbohydrate sulfotransferase 3"
                     /db_xref="GeneID:53374"
                     /db_xref="MGI:MGI:1858224"
     exon            1..420
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /inference="alignment:Splign:2.1.0"
     exon            421..573
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /inference="alignment:Splign:2.1.0"
     exon            574..819
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /inference="alignment:Splign:2.1.0"
     CDS             680..2098
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /EC_number="2.8.2.17"
                     /EC_number="2.8.2.21"
                     /note="chondroitin 6-sulfotransferase; chondroitin
                     6-O-sulfotransferase 1;
                     galactose/N-acetylglucosamine/N-acetylglucosamine
                     6-O-sulfotransferase 0; carbohydrate (chondroitin
                     6/keratan) sulfotransferase 3"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 3"
                     /protein_id="NP_058083.3"
                     /db_xref="CCDS:CCDS23871.2"
                     /db_xref="GeneID:53374"
                     /db_xref="MGI:MGI:1858224"
                     /translation="MEKGLALPQDFRDLVHSLKIRGRYVLFLAFVVIVFIFIEKENKI
                     ISRVSDKLKQIPHFVADANSTDPALLLSENASLLSLSELDSTFSHLRSRLHNLSLQLG
                     VEPAMESQEAGAEKPSQQAGAGTRRHVLLMATTRTGSSFVGEFFNQQGNIFYLFEPLW
                     HIERTVFFQQRGASAAGSALVYRDVLKQLLLCDLYVLEPFISPPPEDHLTQFLFRRGS
                     SRSLCEDPVCTPFVKKVFEKYHCRNRRCGPLNVTLAGEACRRKDHVALKAVRIRQLEF
                     LQPLVEDPRLDLRVIQLVRDPRAVLASRIVAFAGKYENWKKWLSEGQDQLSEDEVQRL
                     RGNCESIRLSAELGLRQPAWLRGRYMLVRYEDVARRPLQKAREMYSFAGIPLTPQVED
                     WIQKNTQATRDSSDVYSTQKNSSEQFEKWRFSMPFKLAQVVQAACGPTMHLFGYKLAR
                     DAASLTNRSISLLEERGTFWVT"
     exon            820..6176
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6148..6153
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /note="hexamer: AATAAA"
     regulatory      6160..6165
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /note="hexamer: AATGAA"
     polyA_site      6172
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /note="major polyA site"
     polyA_site      6176
                     /gene="Chst3"
                     /gene_synonym="C6ST; C6ST-1; GST-0"
                     /note="major polyA site"
ORIGIN      
        1 atcctcttcg agaggccggg tgggcggccg cgccaacttc accgagcgtc cccaagttgc
       61 cgggtgcgcg tgtggagtga ggtggggagt ctggaggaga ggcgcggggc tcccaaagtt
      121 gcagctgccg gctcaggagg ccgcctccgg tcgctagcgg gatcggagtc gtgggtagga
      181 ccgatcgttc gcctgcagct aggacaccgc ccgcctggcg ccgcttcccg cccagcgagg
      241 ggaccagccc ggccgcgcct ccctccccgc actagccgag gacgcagagt gggcgccgtg
      301 gggagcgcag ccccagcatt ttctgagccg agagcgcagt tgggggcgga tgctgccggg
      361 gctggacgcc gacctccgga gtgtcggatc cgcccgccgg gaggaggagg ctcgaccggg
      421 ggattcagga accccggctc cctgcttcct actgggtgac acagagggat cagccctctg
      481 gcctcacaac tttcccacag cagccagatc ttccctctgc ctgtggagcc ccgtggagcc
      541 gcgttttttc tacttggaaa gctgtatata caggatcaga gtgtccccca cctgaagagg
      601 gctgattggg tcccaagcta tgctcctgag ctgagtgcct gcagccagtc tgaggaactc
      661 catggcgccc cctctcccca tggagaaagg actcgctttg cctcaggatt tccgggacct
      721 tgtacacagc ctaaagattc gaggcagata cgtcttgttc ctggcatttg tggtcatagt
      781 ttttatcttc attgaaaagg aaaataaaat catatccagg gtctccgaca agctgaagca
      841 gatccctcat tttgtggcag atgccaacag cactgaccca gccctgctct tatcggagaa
      901 tgcatctctc ttgtccctga gcgagttgga ttccaccttt tcccatctgc ggagccgcct
      961 gcacaacctg agcctgcagc tgggcgtgga gccagcaatg gagagccagg aggctggggc
     1021 agagaagcca tcccagcagg ctggagcagg gacccggcgc cacgtgcttc tcatggccac
     1081 cacccgcacg ggttcctcgt tcgtgggcga gttcttcaac cagcagggca atatcttcta
     1141 cctcttcgag ccactgtggc acatcgagcg caccgtgttc ttccagcagc gaggcgccag
     1201 cgcggctggt tcagccttgg tctaccgtga tgtcctcaag cagttgttgc tatgcgacct
     1261 gtatgtgctg gagcccttca tcagccctcc gcccgaggac cacttgactc agttcctgtt
     1321 ccgccgggga tccagccgtt cactctgcga ggatccggtg tgcacaccct tcgtcaagaa
     1381 ggtctttgag aagtaccact gcaggaaccg tcgctgcggg ccactcaacg tgaccttggc
     1441 gggcgaggcc tgccgccgca aggaccacgt ggccctcaag gctgtgcgca tccgtcagct
     1501 ggagttcctg cagccgctag ttgaggaccc gaggttggat ctacgagtca ttcagctggt
     1561 gcgcgacccc cgggccgtgc tggcttcacg catagtggcc tttgcgggca agtatgagaa
     1621 ctggaagaag tggctgtccg aggggcagga ccagctgagc gaggatgagg tgcagcgatt
     1681 gcggggcaac tgtgagagca tccgcctgtc tgcagagctg ggcttgcggc agccagcctg
     1741 gctgcgcggt cgttacatgc tggtgcgcta tgaggatgtg gcacgcaggc cactgcagaa
     1801 ggcccgagag atgtacagct ttgcgggcat ccccttgacc ccgcaggtgg aggactggat
     1861 ccagaagaac acgcaggcga cacgcgacag cagcgatgtc tactccactc agaaaaactc
     1921 ttctgagcag tttgagaagt ggcgcttcag catgcctttc aagctggcac aggtggtaca
     1981 ggctgcctgt ggcccgacca tgcacctctt tggctacaag ttggccaggg atgccgcctc
     2041 actcaccaac cgctccatca gcctgctgga ggagcggggc accttctggg tcacgtagtg
     2101 ggggatgtct gggacccttg gaactccttc ttgtgaaagg ctggccctgc ttccctcaca
     2161 cccagcctgg cagtgagaca tagccctggc agaaagtcaa aatggggagc gatgatggga
     2221 acatagcccc tggctgtagc ggtagggccc cctgccagct agactcccca gagcagctac
     2281 agctagggcc ttgggctcct ctgaggacac cctgtctcct tagtggtact ggtcataagg
     2341 tgtcctcacc ctatgacctg cagtgtcccg agcaggtcaa ggtaggttcc tgtgtgtgga
     2401 cacacctccc tagctctttc tcacacagtc tacacgaagc ctgtaaaggc ctgtaggttg
     2461 tgtgggctgg agtccaagta tttaacaacc agaaggggtg tagaccctct gcagcctctc
     2521 agacctccta ctgtattaag tgttaacctc ttccgctctg agtcagagaa gctgggatct
     2581 ggctgagtcc tgggaaggag gaggacagcc tagggtgagg aaggggacct ttgaagctcc
     2641 tcagggaaca gtggctgtgt accagctcat agaaaatggt ttgatcagct gttctagtca
     2701 ctcatgagta tcaatcagcc tgtgtagagc aagacacaca aagtgcattg aaaacagaca
     2761 aggccagggg tgtggctcag cggtaaagcc cttgcctctc tcatgcccag ggccctaggt
     2821 gtgattctta gcactataaa tataagggaa aataagccat acatacgtgt gcagtacaga
     2881 gagagacaga aaagcgtaat aaacattccc ccctctcata agcacacgct ctgcctctgt
     2941 ctctgtctct gtctctctct gtctctgtct ctctctgtct ctctctctca ctctctctct
     3001 ctctctctct ctctcacaca cacacacaca cacacacaca caaacacaca cacacacact
     3061 ggtacagaaa agcttccctt ctccactctt tatgctcacc atgtttttag gatttttgtt
     3121 tgtttgtttt gttttgggtt tctatatatc taaacaagga gccttaaatt acatcttttt
     3181 gggattacgt ggcgaggggg agcagcaggg agcatttgct tctggtaaaa ggatagacca
     3241 gaaaggactt ccccttggat gatcggagtc atgcagagcc cccagggtcc cacatgttct
     3301 gtgtgaactt catgtggaat gactcacaga agtgactacc ttaggcgctc gctctgtagc
     3361 tcaatgtgcg tagagtttgt ctattgtata ggaagccctg ggttctgtgc cctgccccac
     3421 accatatagg tatgatggcg tgtacccata tcccagcatt caggaaatca aaggttggcg
     3481 gtcaaaagtt caaggtgcag ctatgtatct tttgagatgt ccgagtctat aagctccctc
     3541 tactgagagc tgctagtatt ccttctcaag aacaggttat ttgaaaatct cttcttgtaa
     3601 gccccaacat ttgggggagg ggaggatggg ggaactcttg actgagcagc taggtttccc
     3661 caagcagaag ttcttctctc ccagtgcaag ctgaggttta gtgccccggc tagcctcgga
     3721 actggctcag tgggggttgg cacgctgact tcctggacaa ttgctgcatc tgacactgtg
     3781 agcgcccagg acaccggatg tgtagcatgg atgtgggcct agcacaggtg tggaagagga
     3841 cagtcaggac actcactgtt ggtgtttgtt atccacctgt tcagccgccg ggtcccacac
     3901 ataggtcatc tttcgtcact caagccggcc tctgtttttg attttaacaa tccaagagca
     3961 agtgtgtagg ggacaaagag acaccagctc ttcatacttg atggagaccg ggacaggatg
     4021 ctgcaggcag gctcgggagt gcattatcgt ttcttctgag ctcttcatcc aggccatatt
     4081 cccttttctg catcgcatct ggggtgggag gggctgagct gggagtttcc gtccttcttc
     4141 cctgtggggg tgggagatgg ggctcaaggt ctccatctct cggctccctg aggacagaac
     4201 ccccacagtg gacctttggg ccttctcagg acattgacaa tgttgtgtgc actgcaagtt
     4261 gactttattt attttgtagg aaaaagagat ggtattcttt agcaggatct gaaactgtac
     4321 cctagtcaag aagtacaatt aataacatta ttattaataa caattgggat gattcaaagg
     4381 tcacacacac accagaccac ccaagaacca tgtaggagat gaggcggagc caggtcatgg
     4441 aagcccagaa tgctacagag attctgtggg tctattattg atcagaaaat acaacatggg
     4501 ggggcctttt ctatggcata actcaggtgt ctgcaaagac agagcagctc aggtgggtgg
     4561 ataggcaggc agatggagcc ttgaatcaca tcccctgagg ctcagccagc cagtgcgaat
     4621 cctcaaggtt cggaaaggga cagtgtagcg gcaaactact gggcatcttc tctgatgctt
     4681 agaggctctt accaaggagc ttgatggaga aagtggccat gttggtggca tgggacaact
     4741 gctcacccaa cttcaaagat caaagggcac ccaggtggcc tatgacagtg acactctttc
     4801 ctaccattag gggtgtctgc ccccactgaa agccatgaaa tttctggccc taagggggta
     4861 ggaaggactt aggagtagca gatggtttga tcccatcccc cccacacaca cacacacatc
     4921 cctcagctgt cttccacatt agagccactt cagttgtcca tggacttgtc cctttgagac
     4981 atcctggatt ttgaaggata gaaacatccc caagatggtc tcgtgttaat cccacaacag
     5041 aggcagaagg gtcatactga gagagagaga gagagagaga gagagagaga gaggagtggg
     5101 ggccagaccc tcaagaagcc aagtgggtct ggtcaacctg tgcacatgag aaggagggaa
     5161 catcactaaa atcagggcct gggctggtgt gttgttggtg agatcccgtg gagtggctgg
     5221 ctagatatgg atgagttttc tgagcatgct cacacacccc caacttcaca ttcttagaaa
     5281 tagcacaacc ataatgcctt acctcaaaag gatgaggcaa agcttgcaat taattcccag
     5341 tgtctgaaga cctccgggat cctttgagct gtgtgtcctt gtcagtacat ggggacaggc
     5401 tccttaggta ttcctgacat agaggtaagg tgctgccctt gctgccttgc ataagctgtg
     5461 acaagcttct tactgggcac atagaacggc tctgtcatct gcttccacta aatttgggct
     5521 tggactttgc ctcctgccaa atctccatct ctgctggata gtctagtccc tagggtctaa
     5581 ccaccaccct ccacttcctg gtgggtccta caagcgctgt ctttgtgcca gtacccggat
     5641 ggtgtcctgc ctcatggcta aatggtacag gacatcttcc cagactgagt ggtcatggca
     5701 tgtgcatgta tgttcatgag tacatgcatg gtgtgtgggg gtggatgggt gcattctgtt
     5761 ctgcttctgg tgcagctaca cagccacaac ctctttctgt cattgacctt cttggtcttc
     5821 ttgtagccac agataatctt ccaatgcccg attctgctgt tctcatctga gagctgacaa
     5881 cccagcgctc agagtagagt tcatgaccta ggaaaccctt ctcctggtag cttactgaac
     5941 ttatttaatt aaaaacgaac atataggggt tggagagatg gttcattggt taagagcacc
     6001 ggctgctttt gcaaaggacc tgagttcaaa tcccagcaac catatggtgg cttacaacca
     6061 tctgtaatga gttctggtgc cctcttctgg catgcaggtg tacatgcaga tagagcactc
     6121 ctatgcataa aataaataaa tcttttaaat aaataaataa atgaacatgc cataaa
//
