LOCUS       NM_018763               6256 bp    mRNA    linear   ROD 02-JUL-2024
DEFINITION  Mus musculus carbohydrate sulfotransferase 2 (Chst2), mRNA.
ACCESSION   NM_018763 XM_001471695
VERSION     NM_018763.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Liu,W., Xiong,W., Liu,W., Hirakawa,J. and Kawashima,H.
  TITLE     A novel monoclonal antibody against 6-sulfo sialyl Lewis x glycans
            attenuates murine allergic rhinitis by suppressing Th2 immune
            responses
  JOURNAL   Sci Rep 13 (1), 15740 (2023)
   PUBMED   37735247
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6256)
  AUTHORS   Zhang,D., Zhang,Y., Zou,X., Li,M., Zhang,H., Du,Y., Wang,J.,
            Peng,C., Dong,C. and Hou,Z.
  TITLE     CHST2-mediated sulfation of MECA79 antigens is critical for breast
            cancer cell migration and metastasis
  JOURNAL   Cell Death Dis 14 (4), 288 (2023)
   PUBMED   37095090
  REMARK    GeneRIF: CHST2-mediated sulfation of MECA79 antigens is critical
            for breast cancer cell migration and metastasis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6256)
  AUTHORS   Pal,S., Dwivedi,D., Pramanik,T., Godbole,G., Iwasato,T.,
            Jabaudon,D., Bhalla,U.S. and Tole,S.
  TITLE     An Early Cortical Progenitor-Specific Mechanism Regulates
            Thalamocortical Innervation
  JOURNAL   J Neurosci 41 (32), 6822-6835 (2021)
   PUBMED   34193558
REFERENCE   5  (bases 1 to 6256)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6256)
  AUTHORS   Hemmerich,S., Lee,J.K., Bhakta,S., Bistrup,A., Ruddle,N.R. and
            Rosen,S.D.
  TITLE     Chromosomal localization and genomic organization for the
            galactose/ N-acetylgalactosamine/N-acetylglucosamine
            6-O-sulfotransferase gene family
  JOURNAL   Glycobiology 11 (1), 75-87 (2001)
   PUBMED   11181564
REFERENCE   7  (bases 1 to 6256)
  AUTHORS   Sakaguchi,H., Kitagawa,H. and Sugahara,K.
  TITLE     Functional expression and genomic structure of human
            N-acetylglucosamine-6-O-sulfotransferase that transfers sulfate to
            beta-N-acetylglucosamine at the nonreducing end of an
            N-acetyllactosamine sequence
  JOURNAL   Biochim Biophys Acta 1523 (2-3), 269-276 (2000)
   PUBMED   11042394
REFERENCE   8  (bases 1 to 6256)
  AUTHORS   Hiraoka,N., Petryniak,B., Nakayama,J., Tsuboi,S., Suzuki,M.,
            Yeh,J.C., Izawa,D., Tanaka,T., Miyasaka,M., Lowe,J.B. and Fukuda,M.
  TITLE     A novel, high endothelial venule-specific sulfotransferase
            expresses 6-sulfo sialyl Lewis(x), an L-selectin ligand displayed
            by CD34
  JOURNAL   Immunity 11 (1), 79-89 (1999)
   PUBMED   10435581
REFERENCE   9  (bases 1 to 6256)
  AUTHORS   Uchimura,K., Muramatsu,H., Kaname,T., Ogawa,H., Yamakawa,T.,
            Fan,Q.W., Mitsuoka,C., Kannagi,R., Habuchi,O., Yokoyama,I.,
            Yamamura,K., Ozaki,T., Nakagawara,A., Kadomatsu,K. and Muramatsu,T.
  TITLE     Human N-acetylglucosamine-6-O-sulfotransferase involved in the
            biosynthesis of 6-sulfo sialyl Lewis X: molecular cloning,
            chromosomal mapping, and expression in various organs and tumor
            cells
  JOURNAL   J Biochem 124 (3), 670-678 (1998)
   PUBMED   9722682
REFERENCE   10 (bases 1 to 6256)
  AUTHORS   Uchimura,K., Muramatsu,H., Kadomatsu,K., Fan,Q.W., Kurosawa,N.,
            Mitsuoka,C., Kannagi,R., Habuchi,O. and Muramatsu,T.
  TITLE     Molecular cloning and characterization of an
            N-acetylglucosamine-6-O-sulfotransferase
  JOURNAL   J Biol Chem 273 (35), 22577-22583 (1998)
   PUBMED   9712885
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ172365.1, BM947059.1, BM950407.1, BC051963.1, BU701895.1,
            AK048226.1, AK034634.1 and AW121776.1.
            
            On or before Aug 8, 2008 this sequence version replaced
            XM_001471695.1, NM_018763.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051963.1, AB011451.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                CJ172365.1         3-15
            14-576              BM947059.1         4-566
            577-613             BM950407.1         555-591
            614-3071            BC051963.1         1-2458
            3072-3467           BU701895.1         215-610
            3468-4589           AK048226.1         504-1625
            4590-6118           AK034634.1         2-1530
            6119-6256           AW121776.1         1-138               c
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 50.11 cM"
     gene            1..6256
                     /gene="Chst2"
                     /gene_synonym="C130041E03Rik; Chts2; GlcNAc6ST; Gn6st;
                     GST-2"
                     /note="carbohydrate sulfotransferase 2"
                     /db_xref="GeneID:54371"
                     /db_xref="MGI:MGI:1891160"
     exon            1..694
                     /gene="Chst2"
                     /gene_synonym="C130041E03Rik; Chts2; GlcNAc6ST; Gn6st;
                     GST-2"
                     /inference="alignment:Splign:2.1.0"
     exon            695..6242
                     /gene="Chst2"
                     /gene_synonym="C130041E03Rik; Chts2; GlcNAc6ST; Gn6st;
                     GST-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             877..2469
                     /gene="Chst2"
                     /gene_synonym="C130041E03Rik; Chts2; GlcNAc6ST; Gn6st;
                     GST-2"
                     /note="N-acetylglucosamine-6-O-sulfotransferase; gn6st-1;
                     glcNAc6ST-1; N-acetylglucosamine 6-O-sulfotransferase 1;
                     galactose/N-acetylglucosamine/N-acetylglucosamine
                     6-O-sulfotransferase 2"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 2"
                     /protein_id="NP_061233.2"
                     /db_xref="CCDS:CCDS52888.1"
                     /db_xref="GeneID:54371"
                     /db_xref="MGI:MGI:1891160"
                     /translation="MSRSSPRALPPGALPRPLPAAPAAVQRALLPPWPRRAGRRWPAS
                     PLGMKVFRRKALVLCAGYALLLVLTMLNLLDYKWHKEPLQQCNPDGPLGAAVGAAGAG
                     WGRPGSPPAAPPRAHSRMDPRTPYRPPAAGVGAVPAAAAGSAGAAASLGNATRGTRGG
                     GDKRQLVYVFTTWRSGSSFFGELFNQNPEVFFLYEPVWHVWQKLYPGDAVSLQGAARD
                     MLSALYRCDLSVFQLYSPAGSGGRNLTTLGIFGAATNKVVCSSPLCPAYRKEVVGLVD
                     DRVCKKCPPQRLARFEEECRKYRTLVIKGVRVFDVAVLAPLLKDPALDLKVIHLVRDP
                     RAVASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGMGGPADYHAL
                     GAMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPE
                     MEQFALNMTSGSGSSSKPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGY
                     ERVNSPEEVKDLSKTLLRKPRL"
     regulatory      3049..3054
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chst2"
                     /gene_synonym="C130041E03Rik; Chts2; GlcNAc6ST; Gn6st;
                     GST-2"
                     /note="hexamer: AATAAA"
     polyA_site      3071
                     /gene="Chst2"
                     /gene_synonym="C130041E03Rik; Chts2; GlcNAc6ST; Gn6st;
                     GST-2"
                     /note="major polyA site"
     regulatory      6219..6224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chst2"
                     /gene_synonym="C130041E03Rik; Chts2; GlcNAc6ST; Gn6st;
                     GST-2"
                     /note="hexamer: AATAAA"
     polyA_site      6242
                     /gene="Chst2"
                     /gene_synonym="C130041E03Rik; Chts2; GlcNAc6ST; Gn6st;
                     GST-2"
ORIGIN      
        1 ggagtcgggt agcagcatcc tcccgggcgc cgctttagtg cggccgcggc gggggcttcc
       61 tctgcctcgg gacctcgtag ggcagaagcc gctggtgacg cgggggtggg gggtcccgct
      121 cttttgcctt cagctaggtg tacagagtcc tgggccagtc cgcggccacg gagcgccggg
      181 gctcgcctga gccgcccgcc aggacatcct ccgggtgatg gaagaagcag ccgccgctgc
      241 ggggccgcgc tgcaccctat ccgcggccgc cggagggatg ggaaggttcg ctacgtggag
      301 gccaagagag agcgatggag agcagaacag cctggagctg gggcgtatgt aagccacccc
      361 ccactgtcgc ctcctgcctg cttcgtgccc cagagcctgg agggaagcag cggtcccgag
      421 agacctaagg agacgtcgct ggagccggga cgcgccgcgc tcggtggagc gcagagctgg
      481 gctccgggcc cggtgaaagt ttcccctccc ggggtgcagg gcgcccgctg ctggaaaact
      541 ctgcctaggg ctagggcagc ggagtctcgc ggctccctcg aaggcttggg gacccctagc
      601 agaagagaac cggagagaaa ccgaggagag tgctagccgg acagtccgcc ggtcggggat
      661 ctggggacgc tccgaggcgc accctccgct ccaggtcctt ctcggagccg ctgccatggg
      721 agagccagcc ctgggcgccg gggaccagca gcctctgccg ccgcgcccgc ctcggatcgg
      781 cggccccagt cccggcgccc gcagccggcc tgcagcgtcc ccctcctggg ctgcagggcc
      841 gcctccgccg cgccgccggc cccggctgtg cctgtgatga gccgcagctc gccgcgagct
      901 ctgccccccg gtgcgcttcc ccggccgctg ccggccgcgc ctgccgccgt gcagcgggcc
      961 ctgctcccgc cgtggccccg gcgcgcagga cgccgctggc ctgcgtcccc gctcgggatg
     1021 aaggtatttc gcaggaaggc gctggtgctg tgcgcgggct atgcactgct actggtgctc
     1081 acgatgctca acctcttgga ctacaagtgg cataaagagc cgctgcagca gtgcaacccc
     1141 gacgggcctc tgggtgccgc ggtaggggcg gccggggccg gctggggacg gccggggtcg
     1201 cctcctgcag cgccaccccg cgctcactct cgcatggacc cccgcacccc gtaccgccct
     1261 cctgccgcgg gcgtgggggc agttcccgca gccgcggctg ggagtgcagg agctgcggcc
     1321 tctctgggca atgctactcg aggcaccagg ggtggagggg acaagcggca gttggtgtat
     1381 gtgttcacca cgtggcgctc gggctcgtcc ttcttcggtg agctcttcaa ccagaaccct
     1441 gaggtgttct tcctctatga gcctgtgtgg cacgtgtggc aaaaactgta ccccggggac
     1501 gccgtttccc tgcagggggc agcgcgggac atgctgagcg ctctctaccg ctgcgatctt
     1561 tcggttttcc agctgtatag ccccgcaggc agtggggggc gcaacctcac cactctgggc
     1621 atctttgggg cagccactaa caaggtggta tgctcctcgc cactctgtcc tgcctaccgc
     1681 aaggaggtcg tcggactggt ggacgaccgc gtgtgcaaaa agtgcccacc tcaacgcctg
     1741 gcacgcttcg aggaggagtg tcgcaagtac cgcacgctgg ttatcaaggg cgtgcgggtc
     1801 ttcgatgtgg ctgtgttggc gccgctgctt aaagatccag ccttggacct caaggtcatc
     1861 cacctagtac gtgatcctcg tgctgttgcc agctcccgca tccgctcgcg tcacggcctc
     1921 atccgggaaa gcctacaggt ggtgcgaagc cgggatccaa gagcccaccg catgcccttc
     1981 ctggaggctg ctggccacaa gcttggtgcc aagaaggagg gtatgggtgg cccagcagac
     2041 taccacgctc tgggtgcaat ggaggtcatc tgcaacagta tggccaagac gctgcaaaca
     2101 gccctgcagc ctcctgactg gctgcaggga cactacttgg tggtgaggta cgaggatctg
     2161 gtgggagacc ccgttaagac cctacggagg gtatatgact ttgtggggct gctggtgagt
     2221 cccgaaatgg agcagtttgc cctgaacatg accagtggtt cgggctcctc ctccaagcct
     2281 ttcgtggtgt cagctcgcaa tgccactcag gccgccaatg cctggcggac cgcgctcacc
     2341 ttccagcaga tcaaacaggt ggaggagttt tgctaccagc ccatggccgt gctgggctat
     2401 gagcgggtta acagtcctga ggaggtcaaa gacctcagca agaccttgct caggaagccc
     2461 cggctttgag aagggttccc aagagatctg acactctccg gagacaccca caaaaaggat
     2521 ggtgttgtgt ttaaacaaac acagcccaga cccaagctga ggaagcccac atattctatt
     2581 atagatatat aatataaata accacacagg cacttgctgt caacgttttg agtcagtgca
     2641 tttcaaggaa cagccctcaa ctcacacgtg caaacacaca cacacacaca cacacacaca
     2701 cacacacaca cacacacaca cacacacccc tcagaaaagg caagacttga aagttctgac
     2761 agattgccct gtgtccctgt ctcttctccc tctctccttc ctccctcttt ccctcctccc
     2821 ttccatattg aagtggaatg ttgataaatc aagttccagt aacccaaatc ttgtttacaa
     2881 aattttcgtg gtatctgtga atgtgtaaga gtaatttgga tgtgggatgg ggtgggtgga
     2941 gaagggggaa gtgaccccca gagcagaaag cccccactgg gctggtaaat caaggaggca
     3001 ggtcttcaaa gtagactttt gtgtcagcaa aggttatatg tgagtattaa taaagaagat
     3061 aataaataat agtcttttta tatttgattc cattactccg gctttaccag tgttacttgt
     3121 ctctgtagct tcaggtggac cctgtccctc catttccagc catctacttg ctaagctggg
     3181 acaagcctct gccttcttac taaccgagag ttgttttctc acatgtaaag cgttgcctgc
     3241 caggtaccca ggaatggagc aaatctacag gcaaaaccaa acagggtaga gaagaaagag
     3301 cacacactac tctcctgttc agctttgagt ccagaaagga agaacaagcc ctggagagtt
     3361 gaaggcattc ggttttgatt actgctgtat tgagttgaga cagggtacct cgaagcagag
     3421 actgctggtt ttgttctgat gttggttcag gggggaaaaa aaaaaaagga gaatttgaaa
     3481 gactttgaaa gcatacaatt tttttttttt taaacaagtt ctgatgacta gaatacagtt
     3541 ttcaaaacga ggtgatcact tttggcagtg gtagcagcaa cttctgtttg ttagtgggat
     3601 aagagaaacg tctgtctgga gtgtattctg tctatgacac agtaatcaga ggcatatgaa
     3661 agcaaacacg ttttaactgg gacactccta ggttttcttt ctcctctgtc ccatccaaat
     3721 taatgatatt aaagggcagg gatttgaact catttacttg ggacttgggg tgtcctggag
     3781 atatgatgtt ctctttattt ccttaaggca aatttgccaa gcgatgaaca tgttgcagac
     3841 agtgtagggc tttagctaag aacgtttgga tactggaatg atcccaagga agaggaaata
     3901 gatgcggctt agtgtgtagg attttgaaat ttgctgactt tcagtgagaa aactattgat
     3961 cttcccagca gcggttatga tactcctatg tctgtgtcct gaactcatat tgtgctgatg
     4021 tgttctgtat ggtgtcatgt tacgataaga aattatgaag cccaaatagg aaaaacagac
     4081 agtatagttg tatattgtat atcatatcaa acacggtaca agtccagcat ccttgcagct
     4141 ttgttttaat gtaatttatt ataagattca ggaaagctaa caagtgtggg ttaaatgctg
     4201 aggacttctt aaaaaaataa tatgtatata ggaaatgttt taacaggagg tgtttttgaa
     4261 tttaattatc attacaatgt aaaattcaag atgaagaaac acttaaaaaa aggtgtagta
     4321 gaacggttat ttcttaattt actaaaattg ttgctcatgt cttgtattga gctagacttt
     4381 ctaactctaa tgctggtcaa gatttccatg gtgtttttat ggtcgtgcct tcagcagcag
     4441 gggaatctat gtaagagctc taatttaaga atactttaaa attaaatgaa caaaataaat
     4501 attaatgctg ggtgactaaa gatgaagaag gcttagcagc aagttaaggt agggtcatta
     4561 aaaggcaatg actaggcata acataaccat ttaaaaacaa attttataag gcattagaat
     4621 cagctcttat cagccacagt gaaattcatt tcttgttgag caaaaagatt tttaagcagt
     4681 tgacttcatt tttctccaat atatccacac acttggattg cagtagaata taattatgta
     4741 tttctatctt gtatttaaga cgtattagat gctctacaga gccttgaggc ttacagagca
     4801 tttgtgacct ctgctaagag gaaattctca gctaccacca acagctctcc ctgcctctgt
     4861 ttgcagagtt tctgtctgta tttttccttg tgtgtgtgtg tgtgtttttt tttttttttt
     4921 ttttttaagc tgccttcttt ccactctgcc actcttgggc acctcaattt gaaaaggtga
     4981 attgttgctc ctggagattt gtaccttact gcattctagg aaagtgagat gggtgttgtt
     5041 cccactagag gctgagatac tcatatccgt gacatttagc tgccagagct tgggagaagg
     5101 ggggaggcat ttttggcgtt aacaagctct tgtcagcccc tggtgcaagc cgacctgtgc
     5161 catgacaaac tgcaacagag ctgtgaccgt ttctgaggca gcgtgtatga aagccagcct
     5221 ttcacatttc ctaacctggc aggatgtcca tcagtgggag gaagtagtcc tcagagcagt
     5281 ttatctatca gattgtgttt gtaagttctg cctgactggt agcacgcctg gcttggccct
     5341 tagactttgg aatgtgtttg ctaattagaa agttccattt agagaatgaa gtgtaggcag
     5401 tacatttagt caacaataag agtttggacc aagacagggg cacaggctgg gtgcttgagt
     5461 ttcaggaatt tcagaggcaa tcaaaggctg catttacact gaccctgagt acagggacag
     5521 gagcaaattg gtgtcctcca gtgtctacac acaacaagtg cttgctaaat acttgttaag
     5581 taagtgaatg aaatgtgtaa ctatcaaata cagaaaaggt ttagagacaa agagaggacg
     5641 attgtgttaa cctgtacaga gaaagtgcca tgcttctggg tgagattgtg gtccataggc
     5701 cgtgttgatg cgtggaacaa gcagtggtgc atctgcagcg tttccaaggt gacacagatc
     5761 tttaatcccc accaaggaat tggaaagaaa tgtagaattt attaatgtgg ggaggaaaaa
     5821 aatttcaaaa taaggaatga aacttccagt gctagttttc taaaccaaca cagaagaagg
     5881 gcagggacca gacataaccg tggttcaaaa ttgctcaggt gcctagagat ggtccgtaaa
     5941 gttcattttt gaagaaacaa tatgatgttt atggttttgg gtactgtgaa tgcatagact
     6001 gaagaatcgt ggtgtagact gtggccaaac agagcaatgg ccactgtcag aaagtccatc
     6061 ccactgtgga tgctgagtga gacaagttct gtccaagtct tttttttttt tttttgtcat
     6121 gcactgtata gttgtggctg gattctacta ttggcttgaa atgaatgtga atttgggtga
     6181 agctatgaag ttaatattcc ctgggaactg agcaattaaa taaagatcat ttttgaaaca
     6241 caaaaaaaaa aaaaaa
//
