LOCUS       NM_021528               1802 bp    mRNA    linear   ROD 04-AUG-2023
DEFINITION  Mus musculus carbohydrate sulfotransferase 12 (Chst12), mRNA.
ACCESSION   NM_021528
VERSION     NM_021528.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Rios JJ, Denton K, Russell J, Kozlitina J, Ferreira CR, Lewanda AF,
            Mayfield JE, Moresco E, Ludwig S, Tang M, Li X, Lyon S, Khanshour
            A, Paria N, Khalid A, Li Y, Xie X, Feng JQ, Xu Q, Lu Y, Hammer RE,
            Wise CA and Beutler B.
  TITLE     Germline Saturation Mutagenesis Induces Skeletal Phenotypes in Mice
  JOURNAL   J Bone Miner Res 36 (8), 1548-1565 (2021)
   PUBMED   33905568
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Schaberg E, Theocharidis U, May M, Lessmann K, Schroeder T and
            Faissner A.
  TITLE     Sulfation of Glycosaminoglycans Modulates the Cell Cycle of
            Embryonic Mouse Spinal Cord Neural Stem Cells
  JOURNAL   Front Cell Dev Biol 9, 643060 (2021)
   PUBMED   34169071
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1802)
  AUTHORS   Nakahara M, Tateyama H, Araki M, Nakagata N, Yamamura K and Araki
            K.
  TITLE     Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from
            MSM/Ms mice
  JOURNAL   Mamm Genome 24 (5-6), 228-239 (2013)
   PUBMED   23604909
REFERENCE   4  (bases 1 to 1802)
  AUTHORS   Izumikawa T, Koike T and Kitagawa H.
  TITLE     Chondroitin 4-O-sulfotransferase-2 regulates the number of
            chondroitin sulfate chains initiated by chondroitin
            N-acetylgalactosaminyltransferase-1
  JOURNAL   Biochem J 441 (2), 697-705 (2012)
   PUBMED   21942880
  REMARK    GeneRIF: chondroitin 4-O-sulfotransferase-2 efficiently transferred
            sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of
            non-reducing terminal GalNAc-linkage residues forming chondroitin
            sulfate.
REFERENCE   5  (bases 1 to 1802)
  AUTHORS   Akita K, von Holst A, Furukawa Y, Mikami T, Sugahara K and Faissner
            A.
  TITLE     Expression of multiple chondroitin/dermatan sulfotransferases in
            the neurogenic regions of the embryonic and adult central nervous
            system implies that complex chondroitin sulfates have a role in
            neural stem cell maintenance
  JOURNAL   Stem Cells 26 (3), 798-809 (2008)
   PUBMED   18079434
REFERENCE   6  (bases 1 to 1802)
  AUTHORS   Taniwaki T, Haruna K, Nakamura H, Sekimoto T, Oike Y, Imaizumi T,
            Saito F, Muta M, Soejima Y, Utoh A, Nakagata N, Araki M, Yamamura K
            and Araki K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (bases 1 to 1802)
  AUTHORS   Araki K, Imaizumi T, Sekimoto T, Yoshinobu K, Yoshimuta J, Akizuki
            M, Miura K, Araki M and Yamamura K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB868264.1, AK142155.1 and AJ289132.1.
            
            [WARNING] On Nov 24, 2023 this sequence was replaced by
            NM_021528.4.
            
            On Sep 14, 2007 this sequence version replaced NM_021528.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003201.1, AK142155.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 BB868264.1         61-62
            3-1772              AK142155.1         1-1770
            1773-1802           AJ289132.1         1686-1715
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 79.04 cM"
     gene            1..1802
                     /gene="Chst12"
                     /gene_synonym="C4S-2; C4ST2"
                     /note="carbohydrate sulfotransferase 12"
                     /db_xref="GeneID:59031"
                     /db_xref="MGI:MGI:1929064"
     exon            1..88
                     /gene="Chst12"
                     /gene_synonym="C4S-2; C4ST2"
                     /inference="alignment:Splign:2.1.0"
     exon            89..1786
                     /gene="Chst12"
                     /gene_synonym="C4S-2; C4ST2"
                     /inference="alignment:Splign:2.1.0"
     CDS             168..1427
                     /gene="Chst12"
                     /gene_synonym="C4S-2; C4ST2"
                     /EC_number="2.8.2.5"
                     /note="chondroitin 4-O-sulfotransferase 2; chondroitin
                     4-sulfotransferase 2"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 12"
                     /protein_id="NP_067503.3"
                     /db_xref="CCDS:CCDS19820.1"
                     /db_xref="GeneID:59031"
                     /db_xref="MGI:MGI:1929064"
                     /translation="MTKPRLFRLWLVLGSALMILLIIVYWDNVGTAHFYLHTSLSRPH
                     ILEPLPTQGLVEENVFTSDVDEFLDTLLSSDAKHNDLSRRKTEQPPVPAPSKPVLSHM
                     EENVRGYDWSTHDAHQNPDRDRQQAERRSLLRDFCANASLAFPTKDRSFDDIPNYELN
                     HLIVDDRHGVIYCYVPKVACTNWKRVMIVLSESLLDRGSPYRDPLDIPREHVHNTSTH
                     LTFNKFWRRYGKFSRHLMKVKLKKYTKFLFVRDPFVRLISAFRSKFELENEEFYRKFA
                     VPMLRLYANHTSLPASVSEAFSAGLKVSFANFIQYLLDPHTEKLAPFNEHWRQVYRLC
                     HPCQIDYDFVGKLETLDEDAAQLLRFLKVDSQLHFPPSYRNRTASSWEEDWFANIPLA
                     WRQQLYKLYEADFVLFGYPKPENLLRD"
     polyA_site      1786
                     /gene="Chst12"
                     /gene_synonym="C4S-2; C4ST2"
ORIGIN      
        1 ggggctgcta gcgggaggag gcgatttcgg ctgcagaatc agcatcacca gcaacagcag
       61 cagcggcggt gactgtggcg ggcgctaggt ccgtctccca gggaccatgt cccagctgtg
      121 cacaaggctg aagtgaaggg ccaggagtgg gcccagccca gggcagcatg accaagccgc
      181 ggctcttccg gctgtggctg gtactagggt cggctctcat gatccttttg atcattgtat
      241 attgggacaa cgtgggcacc gcccacttct atctgcacac gtctctctcc aggccacaca
      301 tcctagaacc ccttcccacc cagggattgg tggaggagaa cgtgttcaca tccgacgtgg
      361 atgagttttt ggatactctc cttagttctg acgcaaagca caacgacctt tccaggagaa
      421 aaactgagca gcccccggtg cccgccccca gcaagccagt cttgagccac atggaggaga
      481 acgtgagagg ctacgactgg tccactcatg atgcccatca gaaccctgac cgggacaggc
      541 agcaggccga gaggaggagc ctgctgagag acttctgtgc caacgccagc ctggcattcc
      601 ccaccaagga ccgctctttt gacgacatcc ccaactacga actgaaccac ctgatcgtgg
      661 acgaccgcca cggggtcatc tactgctacg tgcccaaggt ggcctgcacc aactggaagc
      721 gagtgatgat cgtgctgagc gagagcctgc tggaccgggg cagcccctac cgagaccccc
      781 tggacatccc ccgggaacac gtgcacaaca ccagcacgca cctcaccttc aacaagttct
      841 ggcgccgcta cggaaagttc tcccgtcacc taatgaaggt gaagctgaag aagtacacca
      901 agttcctgtt cgtgcgcgac ccctttgtgc gcctcatctc agccttccgc agcaagttcg
      961 agctggagaa cgaagagttt taccgcaagt tcgcggtgcc catgctccga ctgtacgcca
     1021 accacaccag cctgcccgcc tcggtgagtg aggctttcag cgccgggctc aaggtctcct
     1081 tcgccaactt catccagtac ctcctggacc cacacacgga gaagctggcg cctttcaacg
     1141 agcactggcg acaggtgtac cgcctctgcc acccgtgcca gatagactat gacttcgtgg
     1201 ggaagctgga gacgctcgat gaggacgctg cccagctcct gaggttcctc aaggtagact
     1261 cccagctcca cttccccccc agttatcgga acaggacggc cagcagctgg gaggaagact
     1321 ggtttgccaa catccccctg gcatggaggc aacagctcta taaactctac gaggccgact
     1381 ttgttctctt tggctacccc aagccagaaa acctgctcag ggactgagcc cccagaagcc
     1441 ctcacgctgc ccccaacaaa ttgaatggct gtcccatgag gccgtccttt gaggatggga
     1501 ccctgtggcc tcctgggttc tctcctggct tcctttgctt cctggtgtga caggcagagg
     1561 attccacgcc tctcgcatct ggagaccgtg gtacagccaa gacccaagca cctcactctc
     1621 cagagttttg cgctccccac ccccgccctt ttgcaatctg gatttgttta ctccacagcc
     1681 tgtattcatg gaacactgtg ttaaatactg ttttctaaga ttaatatatt tcagatatat
     1741 ttaatacgaa agtgggagga agctggagta aagtgtggca cccgcaaaaa aaaaaaaaaa
     1801 aa
//
