LOCUS       NM_023850               2682 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus carbohydrate sulfotransferase 1 (Chst1), transcript
            variant 2, mRNA.
ACCESSION   NM_023850
VERSION     NM_023850.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Gonzalez-Gil,A., Porell,R.N., Fernandes,S.M., Maenpaa,E., Li,T.A.,
            Li,T., Wong,P.C., Aoki,K., Tiemeyer,M., Yu,Z.J., Orsburn,B.C.,
            Bumpus,N.N., Matthews,R.T. and Schnaar,R.L.
  TITLE     Human brain sialoglycan ligand for CD33, a microglial inhibitory
            Siglec implicated in Alzheimer's disease
  JOURNAL   J Biol Chem 298 (6), 101960 (2022)
   PUBMED   35452678
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2682)
  AUTHORS   Tsutsumiuchi,M., Hoshino,H., Kogami,A., Tsutsumiuchi,T.,
            Yokoyama,O., Akama,T.O. and Kobayashi,M.
  TITLE     Preferential expression of sialyl 6'-sulfo
            N-acetyllactosamine-capped O-glycans on high endothelial venules in
            human peripheral lymph nodes
  JOURNAL   Lab Invest 99 (10), 1428-1441 (2019)
   PUBMED   31148596
REFERENCE   4  (bases 1 to 2682)
  AUTHORS   Kumagai,T., Kiwamoto,T., Brummet,M.E., Wu,F., Aoki,K., Zhu,Z.,
            Bochner,B.S. and Tiemeyer,M.
  TITLE     Airway glycomic and allergic inflammatory consequences resulting
            from keratan sulfate galactose 6-O-sulfotransferase (CHST1)
            deficiency
  JOURNAL   Glycobiology 28 (6), 406-417 (2018)
   PUBMED   29659839
  REMARK    GeneRIF: CHST1 does not contribute to the synthesis of glycan
            ligands for Siglec-F in the airways, although its absence results
            in exaggerated accumulation of airway macrophages and lymphocytes.
REFERENCE   5  (bases 1 to 2682)
  AUTHORS   Paul,A., Crow,M., Raudales,R., He,M., Gillis,J. and Huang,Z.J.
  TITLE     Transcriptional Architecture of Synaptic Communication Delineates
            GABAergic Neuron Identity
  JOURNAL   Cell 171 (3), 522-539 (2017)
   PUBMED   28942923
REFERENCE   6  (bases 1 to 2682)
  AUTHORS   Schilling,K. and Oberdick,J.
  TITLE     The treasury of the commons: making use of public gene expression
            resources to better characterize the molecular diversity of
            inhibitory interneurons in the cerebellar cortex
  JOURNAL   Cerebellum 8 (4), 477-489 (2009)
   PUBMED   19554387
REFERENCE   7  (bases 1 to 2682)
  AUTHORS   Hecht,J., Seitz,V., Urban,M., Wagner,F., Robinson,P.N., Stiege,A.,
            Dieterich,C., Kornak,U., Wilkening,U., Brieske,N., Zwingman,C.,
            Kidess,A., Stricker,S. and Mundlos,S.
  TITLE     Detection of novel skeletogenesis target genes by comprehensive
            analysis of a Runx2(-/-) mouse model
  JOURNAL   Gene Expr Patterns 7 (1-2), 102-112 (2007)
   PUBMED   16829211
REFERENCE   8  (bases 1 to 2682)
  AUTHORS   Yang,J., Rosen,S.D., Bendele,P. and Hemmerich,S.
  TITLE     Induction of PNAd and N-acetylglucosamine 6-O-sulfotransferases 1
            and 2 in mouse collagen-induced arthritis
  JOURNAL   BMC Immunol 7, 12 (2006)
   PUBMED   16772045
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2682)
  AUTHORS   Kawashima,H., Petryniak,B., Hiraoka,N., Mitoma,J., Huckaby,V.,
            Nakayama,J., Uchimura,K., Kadomatsu,K., Muramatsu,T., Lowe,J.B. and
            Fukuda,M.
  TITLE     N-acetylglucosamine-6-O-sulfotransferases 1 and 2 cooperatively
            control lymphocyte homing through L-selectin ligand biosynthesis in
            high endothelial venules
  JOURNAL   Nat Immunol 6 (11), 1096-1104 (2005)
   PUBMED   16227985
REFERENCE   10 (bases 1 to 2682)
  AUTHORS   Hemmerich,S., Lee,J.K., Bhakta,S., Bistrup,A., Ruddle,N.R. and
            Rosen,S.D.
  TITLE     Chromosomal localization and genomic organization for the
            galactose/ N-acetylgalactosamine/N-acetylglucosamine
            6-O-sulfotransferase gene family
  JOURNAL   Glycobiology 11 (1), 75-87 (2001)
   PUBMED   11181564
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CX238855.1, AK134997.1 and AK011344.1.
            
            On Apr 25, 2009 this sequence version replaced NM_023850.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. All three variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CX238855.1, SRR1660815.29862.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-187               CX238855.1         1-187
            188-2595            AK134997.1         62-2469
            2596-2682           AK011344.1         1109-1195
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 51.17 cM"
     gene            1..2682
                     /gene="Chst1"
                     /gene_synonym="2610008E20Rik; C6ST; GST-1; Gst1; KS6ST;
                     KSGAL6ST; KSST"
                     /note="carbohydrate sulfotransferase 1"
                     /db_xref="GeneID:76969"
                     /db_xref="MGI:MGI:1924219"
     exon            1..442
                     /gene="Chst1"
                     /gene_synonym="2610008E20Rik; C6ST; GST-1; Gst1; KS6ST;
                     KSGAL6ST; KSST"
                     /inference="alignment:Splign:2.1.0"
     exon            443..572
                     /gene="Chst1"
                     /gene_synonym="2610008E20Rik; C6ST; GST-1; Gst1; KS6ST;
                     KSGAL6ST; KSST"
                     /inference="alignment:Splign:2.1.0"
     exon            573..2682
                     /gene="Chst1"
                     /gene_synonym="2610008E20Rik; C6ST; GST-1; Gst1; KS6ST;
                     KSGAL6ST; KSST"
                     /inference="alignment:Splign:2.1.0"
     CDS             615..1850
                     /gene="Chst1"
                     /gene_synonym="2610008E20Rik; C6ST; GST-1; Gst1; KS6ST;
                     KSGAL6ST; KSST"
                     /EC_number="2.8.2.21"
                     /note="N-acetylglucosamine-6-O-sulfotransferase 1; keratan
                     sulfate Gal-6 sulfotransferase;
                     galactose/N-acetylglucosamine/N-acetylglucosamine
                     6-O-sulfotransferase 1; carbohydrate (keratan sulfate
                     Gal-6) sulfotransferase 1"
                     /codon_start=1
                     /product="carbohydrate sulfotransferase 1"
                     /protein_id="NP_076339.1"
                     /db_xref="CCDS:CCDS16450.1"
                     /db_xref="GeneID:76969"
                     /db_xref="MGI:MGI:1924219"
                     /translation="MQCSWKAVLLLALASIAIQYTAIRTFTAKSFHTCPGLTDTGLAE
                     RLCEEGPTFSYNLSRKTHVLILATTRSGSSFVGQLFNQHMDVFYLFEPLYHVQNTLIP
                     RFTQGKSPADRRVMLGASRDLLRSLYDCDLYFLENYIKPPPVNHTTNRVFRRGASRVL
                     CSRPVCDPPGSSDLILEEGDCVRMCGLLNLTLAAEACRERSHVAIKTVRVPEVNDLRA
                     LVEDPRLNLKVIQLVRDPRGILASRSETFRDTYRLWRLWYGTGRKPYNLDVTQLTTVC
                     EDFSSSVSTGLMRPSWLKGKYMLVRYEDLARNPMKKTEEIYEFLGIPLDSHVAHWIQN
                     NTRGDPTLGKHKYSTVRNSAATAEKWRFRLSYDIVAFAQNACQQVLAQLGYKMANSEE
                     ELKNPAISLVEERDFRPFL"
     regulatory      2661..2666
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chst1"
                     /gene_synonym="2610008E20Rik; C6ST; GST-1; Gst1; KS6ST;
                     KSGAL6ST; KSST"
                     /note="hexamer: AATAAA"
     polyA_site      2678
                     /gene="Chst1"
                     /gene_synonym="2610008E20Rik; C6ST; GST-1; Gst1; KS6ST;
                     KSGAL6ST; KSST"
                     /note="major polyA site"
ORIGIN      
        1 caaccaccgc tgcttttgac tccgagacgc aaggagggta ctccgtgagg ctctggcctc
       61 cgaggctgct gctgtcctcc ggagactggg gctgcagtgc tcggtgtccc ggtgcctggc
      121 cctgatctct ctctctctct ctctctctct ctctctctct ctctctctct ctctctctct
      181 ctcatcagga aaaggcactg tgcagagcga gccacccgga gctagcgccc ccgagccatg
      241 gagaggggcc cgtgagggac gcgcggaggc ggaggaagag gcttgggagg agggaggccg
      301 aggagcaggg cgcgggagga ggaggatgcc gccgccacct ccaccgccac caccacccgc
      361 gggcccccgg agagcctgac tctagactaa ggatggagcc agcgctgggc gctgcagcgg
      421 ctcccggcgc gcccgccacc aggtaactgc tgtgtgtgct gcatatggtt ggaagaagac
      481 ccctgtcccc agctgtccct ttggagctac cttctccacc tgaaggcctg aggtgactca
      541 cttccatcac aggaccagtt gaattccaga agacccggtg ccttggagca acagccccat
      601 gacggtgccc agccatgcaa tgttcttgga aggctgtcct cctcctcgcc ctggcctcca
      661 ttgccatcca gtacacagcc atccgcactt tcaccgccaa gtctttccac acttgccctg
      721 ggctgacaga tactggcttg gcagagcggc tgtgtgagga gggccctacc ttctcctaca
      781 acctctccag gaagacccac gtcctgatcc tggccaccac acgcagtggc tcttcctttg
      841 tgggccagct cttcaaccag cacatggatg tcttctacct gttcgagccg ctctatcacg
      901 tccaaaacac gctcatcccc cggttcaccc agggcaagag ccccgcagat cgcagggtca
      961 tgctaggtgc cagccgggac ctgctgagga gcctttatga ttgtgatctc tactttctgg
     1021 agaactacat caagccacct ccagtcaacc acaccaccaa cagggtcttc cgcagagggg
     1081 ccagcagggt cctttgctcc cgtccagtgt gtgaccctcc agggtcctct gacctgatcc
     1141 tggaggaggg ggactgtgtg cgcatgtgtg gcttgctgaa cctgaccttg gcagccgagg
     1201 cttgtcgtga gcgcagtcat gtggccatca agactgtgcg ggtgcccgag gtgaatgact
     1261 tgcgggctct agtggaagac cccaggctga acctcaaggt catccagctg gtgcgagacc
     1321 ctcgtggcat cttggcttct cgcagtgaga ccttccggga cacctaccgg ctctggcggc
     1381 tttggtacgg cacagggagg aagccctaca acctggatgt gacacagctg accactgtgt
     1441 gtgaggattt ctctagctct gtgtctactg gcctcatgcg gccctcctgg ctcaagggca
     1501 agtacatgct ggtacgttat gaggacctgg ccagaaatcc aatgaagaag acagaagaga
     1561 tctacgaatt cctgggtatc cccctggaca gtcacgtggc acactggatc cagaacaata
     1621 cgaggggcga ccccactttg ggcaagcaca aatacagcac agtgcgcaac tctgcagcca
     1681 cagctgagaa gtggcgcttc cgcctctcct atgacatcgt ggcctttgcc cagaatgcct
     1741 gccagcaggt gttggctcag ctgggctaca agatggccaa ctcagaggag gaactgaaga
     1801 atccggccat cagcctagtg gaggagcgtg acttccgccc ttttttgtga cctgggtgtg
     1861 gtgggggtgg gaggcaggtg gctctggttt tgctgacgcg gacctactat gggccgtctt
     1921 taactgttgc cttatctccc tcctccctct cccaccctgt ctgtgtatcc attccagccc
     1981 ctgcccactc tcccctttcc ttcgcctctc ccacaccatc tgtgtgtcct tcttgacctt
     2041 gcccacttcc ttttctcctg cctctttttg cccctgaaat ttgcactatg tctctggagg
     2101 ggaacactgg gcagagggcg tatgatgtgg ggttactccc cccaccccat ttagacacaa
     2161 ggatgttggg tctctgtgag gatggggacg atgtttacag gcacccaatc gcacattcat
     2221 tcacacgtgc acacaggcac acgatgagag gcaccccagc acacaactgg tagttttgca
     2281 attgtcttct caaggtaaga ggatggacac aaaggggccg tacctcccca gtttaaggaa
     2341 agggtccatc cccgccccct tcctgctccc tcccctggag tagggcaccc gtccctcctg
     2401 cacacccttg cctggtggtg agcagggttt tactgtgagg tgaattggga ctactttcta
     2461 tttggtttgt ggtgagcttg tctgtctgag tcttgtggct acccctagac cagtaatggc
     2521 taatgaatct tagaagtttc tgattgatct tggggtccct ctgtgctatt tctttgtgcc
     2581 aaaaaaaaaa aaaaaagaca aaaaaaatgt ggatcagttt gcagagtgaa aattgaaatg
     2641 ctttatttgt gttttctgta aataaaaatg tgcagtactg cc
//
