LOCUS       NM_009603               1599 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus cholinergic receptor, nicotinic, epsilon polypeptide
            (Chrne), mRNA.
ACCESSION   NM_009603 XM_001471640
VERSION     NM_009603.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Eguchi,T., Tezuka,T., Watanabe,Y., Inoue-Yamauchi,A., Sagara,H.,
            Ozawa,M. and Yamanashi,Y.
  TITLE     Calcium-binding protein 7 expressed in muscle negatively regulates
            age-related degeneration of neuromuscular junctions in mice
  JOURNAL   iScience 27 (2), 108997 (2024)
   PUBMED   38327785
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Leek,C.C., Soulas,J.M., Bhattacharya,I., Ganji,E., Locke,R.C.,
            Smith,M.C., Bhavsar,J.D., Polson,S.W., Ornitz,D.M. and Killian,M.L.
  TITLE     Deletion of Fibroblast growth factor 9 globally and in skeletal
            muscle results in enlarged tuberosities at sites of deltoid tendon
            attachments
  JOURNAL   Dev Dyn 250 (12), 1778-1795 (2021)
   PUBMED   34091985
REFERENCE   3  (bases 1 to 1599)
  AUTHORS   Ito,K., Ohkawara,B., Yagi,H., Nakashima,H., Tsushima,M., Ota,K.,
            Konishi,H., Masuda,A., Imagama,S., Kiyama,H., Ishiguro,N. and
            Ohno,K.
  TITLE     Lack of Fgf18 causes abnormal clustering of motor nerve terminals
            at the neuromuscular junction with reduced acetylcholine receptor
            clusters
  JOURNAL   Sci Rep 8 (1), 434 (2018)
   PUBMED   29323161
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1599)
  AUTHORS   Missias,A.C., Mudd,J., Cunningham,J.M., Steinbach,J.H., Merlie,J.P.
            and Sanes,J.R.
  TITLE     Deficient development and maintenance of postsynaptic
            specializations in mutant mice lacking an 'adult' acetylcholine
            receptor subunit
  JOURNAL   Development 124 (24), 5075-5086 (1997)
   PUBMED   9362465
REFERENCE   5  (bases 1 to 1599)
  AUTHORS   Witzemann,V., Schwarz,H., Koenen,M., Berberich,C., Villarroel,A.,
            Wernig,A., Brenner,H.R. and Sakmann,B.
  TITLE     Acetylcholine receptor epsilon-subunit deletion causes muscle
            weakness and atrophy in juvenile and adult mice
  JOURNAL   Proc Natl Acad Sci U S A 93 (23), 13286-13291 (1996)
   PUBMED   8917583
REFERENCE   6  (bases 1 to 1599)
  AUTHORS   Sanes,J.R., Johnson,Y.R., Kotzbauer,P.T., Mudd,J., Hanley,T.,
            Martinou,J.C. and Merlie,J.P.
  TITLE     Selective expression of an acetylcholine receptor-lacZ transgene in
            synaptic nuclei of adult muscle fibers
  JOURNAL   Development 113 (4), 1181-1191 (1991)
   PUBMED   1811935
REFERENCE   7  (bases 1 to 1599)
  AUTHORS   Gu,Y., Camacho,P., Gardner,P. and Hall,Z.W.
  TITLE     Identification of two amino acid residues in the epsilon subunit
            that promote mammalian muscle acetylcholine receptor assembly in
            COS cells
  JOURNAL   Neuron 6 (6), 879-887 (1991)
   PUBMED   2054186
REFERENCE   8  (bases 1 to 1599)
  AUTHORS   Gardner,P.D.
  TITLE     Nucleotide sequence of the epsilon-subunit of the mouse muscle
            nicotinic acetylcholine receptor
  JOURNAL   Nucleic Acids Res 18 (22), 6714 (1990)
   PUBMED   2251144
REFERENCE   9  (bases 1 to 1599)
  AUTHORS   Gu,Y., Franco,A. Jr., Gardner,P.D., Lansman,J.B., Forsayeth,J.R.
            and Hall,Z.W.
  TITLE     Properties of embryonic and adult muscle acetylcholine receptors
            transiently expressed in COS cells
  JOURNAL   Neuron 5 (2), 147-157 (1990)
   PUBMED   2383398
REFERENCE   10 (bases 1 to 1599)
  AUTHORS   Buonanno,A., Mudd,J. and Merlie,J.P.
  TITLE     Isolation and characterization of the beta and epsilon subunit
            genes of mouse muscle acetylcholine receptor
  JOURNAL   J Biol Chem 264 (13), 7611-7616 (1989)
   PUBMED   2708381
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from X55718.1.
            
            On Jul 25, 2007 this sequence version replaced XM_001471640.1.
            
            Summary: This gene encodes the epsilon subunit of the
            muscle-derived nicotinic acetylcholine receptor, a pentameric
            neurotransmitter receptor and member of the ligand-gated ion
            channel superfamily. The acetylcholine receptor changes subunit
            composition shortly after birth when the epsilon subunit replaces
            the gamma subunit seen in embryonic receptors. In mice, deficiency
            of this gene can lead to a decline in the number of nicotinic
            acetylcholine receptors at neuromuscular junctions and causes
            progressive muscle weakness, atrophy and premature death. Mutations
            in this gene serve as a pathophysiological model for human
            congenital myasthenia. Several alternatively spliced transcript
            variants of this gene have been described, but their full-length
            nature is not known. [provided by RefSeq, Nov 2012].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X55718.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 43.14 cM"
     gene            1..1599
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /note="cholinergic receptor, nicotinic, epsilon
                     polypeptide"
                     /db_xref="GeneID:11448"
                     /db_xref="MGI:MGI:87894"
     exon            1..65
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..1501
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /note="acetylcholine receptor subunit epsilon; AchR
                     epsilon subunit; nicotinic acetylcholine receptor epsilon
                     subunit"
                     /codon_start=1
                     /product="acetylcholine receptor subunit epsilon
                     precursor"
                     /protein_id="NP_033733.1"
                     /db_xref="CCDS:CCDS24956.1"
                     /db_xref="GeneID:11448"
                     /db_xref="MGI:MGI:87894"
                     /translation="MAGALLGALLLLTLFGRSQGKNEELSLYHHLFDNYDPECRPVRR
                     PEDTVTITLKVTLTNLISLNEKEETLTTSVWIGIDWHDYRLNYSKDDFAGVGILRVPS
                     EHVWLPEIVLENNIDGQFGVAYDSNVLVYEGGYVSWLPPAIYRSTCAVEVTYFPFDWQ
                     NCSLIFRSQTYNAEEVEFIFAVDDDGNTINKIDIDTAAFTENGEWAIDYCPGMIRRYE
                     GGSTEGPGETDVIYTLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVS
                     INVLLAQTVFLFLIAQKIPETSLSVPLLGRYLIFVMVVATLIVMNCVIVLNVSLRTPT
                     THATSPRLRQILLELLPRLLGSSPPPEDPRTASPARRASSVGILLRAEELILKKPRSE
                     LVFEGQRHRHGTWTAALCQNLGAAAPEIRCCVDAVNFVAESTRDQEATGEELSDWVRM
                     GKALDNVCFWAALVLFSVGSTLIFLGGYFNQVPDLPYPPCIQP"
     exon            66..208
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            209..253
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            254..363
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            364..519
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            520..620
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            621..821
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            822..936
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1051
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1238
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            1239..1345
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     exon            1346..1591
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1567..1572
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /note="hexamer: AATAAA"
     polyA_site      1591
                     /gene="Chrne"
                     /gene_synonym="AChrepsilon; Acre; nAChRE"
                     /note="major polyA site"
ORIGIN      
        1 ctgagaacca gacatcagga tggcaggggc tctgcttggt gccctgcttc tcctgacact
       61 ctttggcaga agccagggaa agaatgaaga gcttagcctg tatcaccatc tcttcgacaa
      121 ttatgatcca gaatgccggc cagttaggag acctgaggac actgtcacca tcaccctcaa
      181 ggtcacccta accaacctca tctcactgaa cgagaaagaa gaaactctga ccaccagtgt
      241 ctggattggc attgactggc acgactatcg gctcaactac agcaaggacg attttgcagg
      301 tgtaggaatc ctccgggtcc cttcagaaca tgtatggctg ccagagattg ttctagaaaa
      361 caatattgat gggcagtttg gagtggccta cgacagcaat gttctagtct atgagggagg
      421 ctatgtgagc tggttgcccc cagccatcta ccgcagcacc tgcgcagtgg aggtcaccta
      481 tttccccttt gactggcaga actgctctct catttttcgc tcccagacct acaatgctga
      541 ggaggtggag ttcatctttg ccgtggatga cgacggcaat accatcaaca agattgacat
      601 tgacacggca gcttttaccg agaatggaga atgggccata gactactgcc caggcatgat
      661 tcgccgctat gagggaggtt ccacagaagg tcctggagaa actgacgtca tctatacgct
      721 catcatccgc cggaagccgc ttttttacgt cattaacatc attgtgcctt gcgtgctcat
      781 ttctggcttg gtgctgctcg cttacttcct gcctgcgcag gctggtggcc agaaatgcac
      841 ggtctctatc aacgtcctgc tagcccagac tgtcttcttg tttctaattg cccagaaaat
      901 tccagagact tctctgagcg tgccactgct gggcaggtat cttatattcg tcatggtggt
      961 tgccacgctc attgtcatga attgcgtcat cgtgctcaac gtatctttga ggacgccaac
     1021 gactcatgct acatcccctc ggctgcgcca gattttatta gagctgctgc cgcgtctcct
     1081 gggctcgagc ccacccccag aggatccccg aactgcctca ccagcgaggc gtgcctcgtc
     1141 tgtgggcatt ctgctcagag cggaggagct catcttgaaa aagccgcgga gcgaactcgt
     1201 gtttgagggt cagaggcatc ggcacggaac ttggaccgca gccctctgcc agaacctggg
     1261 tgctgcagcc ccagaaatcc gctgctgtgt ggatgctgtg aactttgtgg ctgagagcac
     1321 aagagaccag gaagccactg gagaggaact gtccgactgg gtgcgtatgg ggaaggccct
     1381 ggacaatgtc tgtttttggg cagctttggt gctcttcagc gttggttcta ctctcatctt
     1441 ccttgggggt tacttcaacc aagttcctga tctcccttac ccaccgtgca tccaaccatg
     1501 agcctgcact ggcacccacc tctcccccac cccccaagaa agagattttg aaaacaggcc
     1561 gctgacaata aatctggttt gtgaacttgc aaaaaaaaa
//
