LOCUS       NM_148944               3486 bp    mRNA    linear   ROD 15-JUL-2024
DEFINITION  Mus musculus cholinergic receptor, nicotinic, beta polypeptide 4
            (Chrnb4), mRNA.
ACCESSION   NM_148944
VERSION     NM_148944.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Tsuzuki,A., Yamasaki,M., Konno,K., Miyazaki,T., Takei,N.,
            Tomita,S., Yuzaki,M. and Watanabe,M.
  TITLE     Abundant extrasynaptic expression of alpha3beta4-containing
            nicotinic acetylcholine receptors in the medial
            habenula-interpeduncular nucleus pathway in mice
  JOURNAL   Sci Rep 14 (1), 14193 (2024)
   PUBMED   38902419
  REMARK    GeneRIF: Abundant extrasynaptic expression of
            alpha3beta4-containing nicotinic acetylcholine receptors in the
            medial habenula-interpeduncular nucleus pathway in mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3486)
  AUTHORS   Jimenez-Pompa,A., Arribas,R.L., McIntosh,J.M. and Albillos,A.
  TITLE     Differential tyrosine and serine/threonine
            phosphorylation/dephosphorylation pathways regulate the expression
            of alpha7 versus alpha3beta4 nicotinic receptor subtypes in mouse
            hippocampal neurons
  JOURNAL   Biochem Biophys Res Commun 684, 149115 (2023)
   PUBMED   37879250
  REMARK    GeneRIF: Differential tyrosine and serine/threonine
            phosphorylation/dephosphorylation pathways regulate the expression
            of alpha7 versus alpha3beta4 nicotinic receptor subtypes in mouse
            hippocampal neurons.
REFERENCE   4  (bases 1 to 3486)
  AUTHORS   Mondoloni,S., Nguyen,C., Vicq,E., Ciscato,M., Jehl,J., Durand-de
            Cuttoli,R., Torquet,N., Tolu,S., Pons,S., Maskos,U., Marti,F.,
            Faure,P. and Mourot,A.
  TITLE     Prolonged nicotine exposure reduces aversion to the drug in mice by
            altering nicotinic transmission in the interpeduncular nucleus
  JOURNAL   Elife 12, e80767 (2023)
   PUBMED   37249215
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3486)
  AUTHORS   Bsharat,S., Monni,E., Singh,T., Johansson,J.K., Achanta,K.,
            Bertonnier-Brouty,L., Schmidt-Christensen,A., Holmberg,D.,
            Kokaia,Z., Prasad,R.B. and Artner,I.
  TITLE     MafB-dependent neurotransmitter signaling promotes beta cell
            migration in the developing pancreas
  JOURNAL   Development 150 (6) (2023)
   PUBMED   36897571
REFERENCE   6  (bases 1 to 3486)
  AUTHORS   Xu,W., Orr-Urtreger,A., Nigro,F., Gelber,S., Sutcliffe,C.B.,
            Armstrong,D., Patrick,J.W., Role,L.W., Beaudet,A.L. and De Biasi,M.
  TITLE     Multiorgan autonomic dysfunction in mice lacking the beta2 and the
            beta4 subunits of neuronal nicotinic acetylcholine receptors
  JOURNAL   J Neurosci 19 (21), 9298-9305 (1999)
   PUBMED   10531434
REFERENCE   7  (bases 1 to 3486)
  AUTHORS   Drescher,D.G., Khan,K.M., Green,G.E., Morley,B.J., Beisel,K.W.,
            Kaul,H., Gordon,D., Gupta,A.K., Drescher,M.J. and Barretto,R.L.
  TITLE     Analysis of nicotinic acetylcholine receptor subunits in the
            cochlea of the mouse
  JOURNAL   Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 112 (3),
            267-273 (1995)
   PUBMED   8838678
REFERENCE   8  (bases 1 to 3486)
  AUTHORS   Nagavarapu,U. and Boyd,R.T.
  TITLE     Distribution of neuronal nicotinic acetylcholine receptor gene
            restriction fragment length polymorphisms in inbred and partially
            inbred mice
  JOURNAL   Pharmacogenetics 5 (5), 326-331 (1995)
   PUBMED   8563774
REFERENCE   9  (bases 1 to 3486)
  AUTHORS   Anand,R. and Lindstrom,J.
  TITLE     Chromosomal localization of seven neuronal nicotinic acetylcholine
            receptor subunit genes in humans
  JOURNAL   Genomics 13 (4), 962-967 (1992)
   PUBMED   1505988
REFERENCE   10 (bases 1 to 3486)
  AUTHORS   Eng,C.M., Kozak,C.A., Beaudet,A.L. and Zoghbi,H.Y.
  TITLE     Mapping of multiple subunits of the neuronal nicotinic
            acetylcholine receptor to chromosome 15 in man and chromosome 9 in
            mouse
  JOURNAL   Genomics 9 (2), 278-282 (1991)
   PUBMED   2004777
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC156832.2.
            
            On May 1, 2007 this sequence version replaced NM_148944.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK140071.1, BC138851.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-101               AC156832.2         67249-67349         c
            102-250             AC156832.2         62582-62730         c
            251-295             AC156832.2         55392-55436         c
            296-405             AC156832.2         55197-55306         c
            406-1396            AC156832.2         53447-54437         c
            1397-3486           AC156832.2         46961-49050         c
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 29.84 cM"
     gene            1..3486
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /note="cholinergic receptor, nicotinic, beta polypeptide
                     4"
                     /db_xref="GeneID:108015"
                     /db_xref="MGI:MGI:87892"
     exon            1..101
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..1537
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /note="neuronal acetylcholine receptor subunit beta-4;
                     nicotinic acetylcholine receptor beta4 subunit"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit beta-4
                     precursor"
                     /protein_id="NP_683746.1"
                     /db_xref="CCDS:CCDS23200.1"
                     /db_xref="GeneID:108015"
                     /db_xref="MGI:MGI:87892"
                     /translation="MRGTPLLLVSLFALLQPGDCRLANAEEKLMDDLLNKTRYNNLIR
                     PATSSSQLISIRLELSLSQLISVNEREQIMTTSIWLKQEWTDYRLAWNSSCYEGVNIL
                     RIPAKRVWLPDIVLYNNADGTYEVSVYTNVIVRSNGSIQWLPPAIYKSACKIEVKHFP
                     FDQQNCTLKFRSWTYDHTEIDMVLKSPTAIMDDFTPSGEWDIVALPGRRTVNPQDPSY
                     VDVTYDFIIKRKPLFYTINLIIPCVLITSLAILVFYLPSDCGEKMTLCISVLLALTFF
                     LLLISKIVPPTSLDIPLIGKYLLFTMVLVTFSIVTTVCVLNVHHRSPSTHTMASWVKE
                     CFLHKLPTFLFMKRPGLEVSPARVPHSSQLHLTTAEATSTSALGPSSPSNLYGNSMYF
                     VNPVPATPKSAVSSHTAGLPRDARLRSSGRFRQDLQEALEGVSFIAQHLESDDRDQSV
                     IEDWKFVAMVVDRLFLWVFVIVCILGTMGLFLPPLFQIHAPSKGL"
     exon            102..250
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /inference="alignment:Splign:2.1.0"
     exon            251..295
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /inference="alignment:Splign:2.1.0"
     exon            296..405
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /inference="alignment:Splign:2.1.0"
     exon            406..1396
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..3486
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2289..2294
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /note="hexamer: AATAAA"
     polyA_site      2309
                     /gene="Chrnb4"
                     /gene_synonym="Acrb-4; Acrb4"
                     /note="major polyA site"
ORIGIN      
        1 gctcactcgc ggttccattg tggggtgacc ggcagccacc cggccggcca tgaggggtac
       61 gcccctgctc ctcgtctctc tgttcgctct gcttcagcct ggggactgcc gcctggccaa
      121 cgcagaggag aagctgatgg atgatctcct gaacaaaacc cgctacaaca acctgatccg
      181 cccagccacc agctcctccc agctcatctc catccgcctg gagctatcac tgtcccagct
      241 catcagtgtg aatgagcgag aacagatcat gaccaccagc atctggctga aacaggaatg
      301 gactgactac cgcctggcct ggaacagctc ctgctatgaa ggggtgaaca ttctgaggat
      361 tcctgcaaag cgagtctggt tgcctgacat cgtgttgtac aacaatgccg atgggaccta
      421 tgaggtgtct gtctacacca acgtgatcgt gcgttccaac ggcagcatcc agtggctgcc
      481 ccctgccatc tacaagagtg cctgcaagat agaggtgaag cactttccct tcgaccagca
      541 gaactgcacc ctcaaatttc gctcctggac ctatgaccac acagagattg acatggttct
      601 taaatcaccc acagccatca tggatgactt tacccccagc ggtgaatggg acattgtggc
      661 cctcccgggt cgaaggacag tgaacccaca ggaccccagc tatgtggacg tgacctatga
      721 cttcatcatc aagcgcaagc cactcttcta caccatcaat ctgatcatcc cttgtgtgct
      781 catcacgtcg ctggctatcc tggtcttcta cctgccctcc gactgtgggg agaagatgac
      841 gctctgcatc tcagtgctgc tggcactcac attcttcctg ctgctcatct ccaagatcgt
      901 gcctcccacc tcccttgaca ttcccctcat tggcaagtac ctcttgttca ccatggtgct
      961 ggtcaccttt tccatcgtca ccactgtgtg tgtcctcaat gtgcaccacc gttcacccag
     1021 cactcacacc atggcatcct gggtcaagga gtgcttcctg cacaagctgc ccaccttcct
     1081 cttcatgaag cgccctggtc tggaagtcag ccccgccagg gtccctcatt ccagccagct
     1141 gcacttgacc acagctgaag ctacgtccac ctctgcctta ggccccagca gcccatccaa
     1201 cctctatggg aattccatgt actttgtgaa ccctgtccct gccactccta agtctgcagt
     1261 cagctcccac acggcaggcc tccccaggga tgcccggctg aggtcctctg ggaggttccg
     1321 gcaagatcta caggaagcat tagagggcgt cagcttcatc gcacagcatt tggagagtga
     1381 tgatcgagat cagagtgtca tcgaggactg gaaattcgtc gcaatggtcg tcgaccgcct
     1441 gttcctgtgg gtgttcgtga ttgtgtgtat tctgggcacc atggggctct tcctgccacc
     1501 cctcttccag atccacgcac cctccaaggg cctctaggct acccagcgtg ttatggggcc
     1561 ctgggttgta gtgggatgat atgagcagcc tagtgggcag tttactgctt catctgggtt
     1621 gtagctaatg agaccctaag taaagctaag actattggca gtcagcctat caatctagcc
     1681 acgagaagga cagagatagg tggcttgcac tgatcgctct gaatatcttc gagtaagtcc
     1741 cttgaaggag ggggattagg tggctgaagt ccattctggc ctccttcctt ccatgtgcca
     1801 ggcactggag tcagacagct gtgtatcatc ccatcacacc ccagcccacc ttcccatcca
     1861 tctccctcca atccaggcta cccactagat tccctggccg tctttcattt cctctcacac
     1921 ttccagattc ttccaggtta gaaccttcaa atctgctgta gctaccgccc aaacctcaga
     1981 ctctcaattc ccaagctttt tacatgtctg cactaggcag agatctactc ccatgatcct
     2041 ctgttccaat atgtcacttc ctgtacaagc cttccctagc catgcattcc aaagcagcgt
     2101 ctcccataac cacattaaac ctccagcttt cttgagtgtc ctccattggc agctgcagtc
     2161 cttagttggt ggcacactag tcaccagcac cttgcccatt aaagtctatg ctcaacaagg
     2221 gtcggagtgt gtgaattcac attcacactc cagagcctgg gacagagctc tgcacacaga
     2281 aggtgcaaaa taaatgtttg ttgaatgaat ggctcctgct ctccttcaaa cacacacaca
     2341 cacacacaca cacacacaca cacacacaca caccctctga gtagctgtcc ttgctccctt
     2401 cctgctttct gcctttacac catggttggt tcaggcctgt ctctttctca cactgatctg
     2461 tttctctcac ggtgcctgcc ccactgttgg cactcaagat acttctggaa ttaaggagtg
     2521 ttagtggctt ttttaaaaaa ataaatgttg agccaggtgt ggtggttcac gcctttagtc
     2581 ccagcacttg ggaggcagag gtaggcggat ctctgagttc gaggccagcc tggtctacag
     2641 agtgagttcc aggacagcca gggctacaca gagaaaccct gtctcataaa aacaaacaaa
     2701 caaacaaaac aatacaaaaa aaatgttgct gttttccttt tacccagcta gctcccaaat
     2761 aattttgact ggactattgt atttatttaa gaagctttaa aggcacaaca acgagcagca
     2821 ttatctattt taatcctcta aactgatctg gctacctccc agtcaaaacc ccagtgatac
     2881 ttgcatttta gtactgatct ggctctctgg gctccaggtg ttttcttatg gcatctcctg
     2941 gactctctac ccaaagagaa tccccactct cctcctgccc ccttccctgg atgggaggaa
     3001 gtccagacct agtctctccc ctgcttagcc attgaccaat cagggaataa ttggggagca
     3061 atgtttacat aacattgaga ctggaaattc tcagaataag aattacaatc aaatgtgggg
     3121 gcacagaaat cagcgtttga ataatataaa ggtaatcttt acacagggta ttatttcatg
     3181 cctacaaggg gaagatttta aaaatttatt attttatgtg tatggatgtt ttgccttcat
     3241 gtatgtgtgt gtaccacgta catgctagta cccccccccc cagaggccag gaaagggcac
     3301 tagatcccct ggaactggag ttacagactt gagccaccac gtatgtggtg ggaatcaaac
     3361 ctgagtcttc tggaagaaca gccaatgctc ttcttgactg ctgagtctcc agcccctgag
     3421 ggagaatatt ttaaagtgtt ttgtttttag aattaaccta caataaagtt gacttcttta
     3481 aagttt
//
