LOCUS       NM_173212               4692 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus cholinergic receptor, nicotinic, beta polypeptide 3
            (Chrnb3), transcript variant 1, mRNA.
ACCESSION   NM_173212
VERSION     NM_173212.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Jackson,A.B., Toma,W., Contreras,K.M., Alkhlaif,Y. and Damaj,M.I.
  TITLE     The beta3 subunit of the nicotinic acetylcholine receptor is
            required for nicotine withdrawal-induced affective but not physical
            signs or nicotine reward in mice
  JOURNAL   Pharmacol Biochem Behav 183, 1-5 (2019)
   PUBMED   31145916
  REMARK    GeneRIF: Beta3 nicotinic subunits do not seem to influence nicotine
            reward but plays an important role in affective nicotine withdrawal
            signs.
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Yan,Y., Peng,C., Arvin,M.C., Jin,X.T., Kim,V.J., Ramsey,M.D.,
            Wang,Y., Banala,S., Wokosin,D.L., McIntosh,J.M., Lavis,L.D. and
            Drenan,R.M.
  TITLE     Nicotinic Cholinergic Receptors in VTA Glutamate Neurons Modulate
            Excitatory Transmission
  JOURNAL   Cell Rep 23 (8), 2236-2244 (2018)
   PUBMED   29791835
REFERENCE   3  (bases 1 to 4692)
  AUTHORS   Henderson,B.J., Wall,T.R., Henley,B.M., Kim,C.H., Nichols,W.A.,
            Moaddel,R., Xiao,C. and Lester,H.A.
  TITLE     Menthol Alone Upregulates Midbrain nAChRs, Alters nAChR Subtype
            Stoichiometry, Alters Dopamine Neuron Firing Frequency, and
            Prevents Nicotine Reward
  JOURNAL   J Neurosci 36 (10), 2957-2974 (2016)
   PUBMED   26961950
REFERENCE   4  (bases 1 to 4692)
  AUTHORS   Srinivasan,R., Henley,B.M., Henderson,B.J., Indersmitten,T.,
            Cohen,B.N., Kim,C.H., McKinney,S., Deshpande,P., Xiao,C. and
            Lester,H.A.
  TITLE     Smoking-Relevant Nicotine Concentration Attenuates the Unfolded
            Protein Response in Dopaminergic Neurons
  JOURNAL   J Neurosci 36 (1), 65-79 (2016)
   PUBMED   26740650
REFERENCE   5  (bases 1 to 4692)
  AUTHORS   Kamens,H.M., Miyamoto,J., Powers,M.S., Ro,K., Soto,M., Cox,R.,
            Stitzel,J.A. and Ehringer,M.A.
  TITLE     The beta3 subunit of the nicotinic acetylcholine receptor:
            Modulation of gene expression and nicotine consumption
  JOURNAL   Neuropharmacology 99, 639-649 (2015)
   PUBMED   26318101
  REMARK    GeneRIF: Data provide evidence that the protective genetic variant
            at rs6474413 identified in human genetic studies reduces gene
            expression and that decreased beta3 gene expression in mice reduces
            nicotine intake
REFERENCE   6  (bases 1 to 4692)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   7  (bases 1 to 4692)
  AUTHORS   Cui,C., Booker,T.K., Allen,R.S., Grady,S.R., Whiteaker,P.,
            Marks,M.J., Salminen,O., Tritto,T., Butt,C.M., Allen,W.R.,
            Stitzel,J.A., McIntosh,J.M., Boulter,J., Collins,A.C. and
            Heinemann,S.F.
  TITLE     The beta3 nicotinic receptor subunit: a component of
            alpha-conotoxin MII-binding nicotinic acetylcholine receptors that
            modulate dopamine release and related behaviors
  JOURNAL   J Neurosci 23 (35), 11045-11053 (2003)
   PUBMED   14657161
REFERENCE   8  (bases 1 to 4692)
  AUTHORS   Kuo,Y., Lucero,L., Michaels,J., DeLuca,D. and Lukas,R.J.
  TITLE     Differential expression of nicotinic acetylcholine receptor
            subunits in fetal and neonatal mouse thymus
  JOURNAL   J Neuroimmunol 130 (1-2), 140-154 (2002)
   PUBMED   12225896
REFERENCE   9  (bases 1 to 4692)
  AUTHORS   Burmeister,M., Novak,J., Liang,M.Y., Basu,S., Ploder,L.,
            Hawes,N.L., Vidgen,D., Hoover,F., Goldman,D., Kalnins,V.I.,
            Roderick,T.H., Taylor,B.A., Hankin,M.H. and McInnes,R.R.
  TITLE     Ocular retardation mouse caused by Chx10 homeobox null allele:
            impaired retinal progenitor proliferation and bipolar cell
            differentiation
  JOURNAL   Nat Genet 12 (4), 376-384 (1996)
   PUBMED   8630490
REFERENCE   10 (bases 1 to 4692)
  AUTHORS   Drescher,D.G., Khan,K.M., Green,G.E., Morley,B.J., Beisel,K.W.,
            Kaul,H., Gordon,D., Gupta,A.K., Drescher,M.J. and Barretto,R.L.
  TITLE     Analysis of nicotinic acetylcholine receptor subunits in the
            cochlea of the mouse
  JOURNAL   Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 112 (3),
            267-273 (1995)
   PUBMED   8838678
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK030464.1, AK133689.1 and BC058193.1.
            
            On Apr 29, 2009 this sequence version replaced NM_173212.3.
            
            Transcript Variant: This variant represents the longer transcript
            and it encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058193.1, AK030464.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                AK030464.1         2-58
            58-237              AK133689.1         2-181
            238-3333            BC058193.1         1-3096
            3334-4499           AK030464.1         3335-4500
            4500-4692           BC058193.1         4262-4454
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 16.0 cM"
     gene            1..4692
                     /gene="Chrnb3"
                     /gene_synonym="5730417K16Rik; Acrb3"
                     /note="cholinergic receptor, nicotinic, beta polypeptide
                     3"
                     /db_xref="GeneID:108043"
                     /db_xref="MGI:MGI:106212"
     exon            1..307
                     /gene="Chrnb3"
                     /gene_synonym="5730417K16Rik; Acrb3"
                     /inference="alignment:Splign:2.1.0"
     CDS             259..1653
                     /gene="Chrnb3"
                     /gene_synonym="5730417K16Rik; Acrb3"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; neuronal acetylcholine receptor subunit beta-3"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit beta-3
                     isoform 1 precursor"
                     /protein_id="NP_775304.1"
                     /db_xref="CCDS:CCDS22217.1"
                     /db_xref="GeneID:108043"
                     /db_xref="MGI:MGI:106212"
                     /translation="MTGFLRVFLALSATLSGSWVTLTATAGLSSVAEHEDALLRHLFQ
                     GYQKCVRPVLNSSDIIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWTDQKLRWNPED
                     YGGINSIKVPSESLWLPDIVLFENADGRFEGSLMTKAIVKSSGTVSWTPPASYKSSCT
                     MDVTFFPFDKQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGN
                     RREGFYSYPFVTYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTS
                     VLVSLTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTY
                     HPMAPWVKRLFLEKLPRWLCMKDPRDRFSFPDGTESKGTVRGKFPGKKKQTPTSDGER
                     VLVAFLEKASESIRYISRHVKKEHFISQVVQDWKFVAQVLDRIFLWLFLTASVLGSVL
                     IFIPALKMWIHRFH"
     exon            308..480
                     /gene="Chrnb3"
                     /gene_synonym="5730417K16Rik; Acrb3"
                     /inference="alignment:Splign:2.1.0"
     exon            481..525
                     /gene="Chrnb3"
                     /gene_synonym="5730417K16Rik; Acrb3"
                     /inference="alignment:Splign:2.1.0"
     exon            526..635
                     /gene="Chrnb3"
                     /gene_synonym="5730417K16Rik; Acrb3"
                     /inference="alignment:Splign:2.1.0"
     exon            636..1518
                     /gene="Chrnb3"
                     /gene_synonym="5730417K16Rik; Acrb3"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..4589
                     /gene="Chrnb3"
                     /gene_synonym="5730417K16Rik; Acrb3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagagatgt ttttagcctc acaagacctg acagctcact gggcatcagt gaagtgcact
       61 ttaaaaaacc cacaccgacg ggaggtgact tgtgactatt ttcatcaggc agctggtcac
      121 gatccccttg ggttgcatcg aggctctgaa ccacttgtat tttgattttt ttttgttttg
      181 ttttgttttt taaaccctgc ttctttccag ttggaaacac tgagcttaaa aggaaatcgt
      241 ctctctgaag cagacgtcat gacaggcttc ctacgggtct tcttggctct cagtgccact
      301 ctctcaggtt cctgggtgac tctcacagcc actgcgggac tcagctcagt ggctgaacat
      361 gaagacgcac tcctcagaca tttgttccaa ggttatcaga aatgtgtccg ccctgtgttg
      421 aattccagtg acatcataaa agtgtatttt ggattaaaga tatctcagct tgtagatgtg
      481 gatgaaaaga atcagctgat gacaacaaac gtgtggctga agcaggaatg gacagaccaa
      541 aaattacgat ggaatcccga agactatggt ggaattaatt cgataaaggt tccatcggag
      601 tcgctctggc tgcctgacat agttctcttt gaaaatgctg acggacgttt tgagggctct
      661 ctcatgacca aggccattgt gaaatccagc ggaaccgtca gttggactcc tcccgccagc
      721 tacaaaagtt cctgtaccat ggatgtcaca tttttcccgt tcgataagca gaactgctca
      781 atgaagttcg gatcctggac ttacgatggt accatggtcg acctcattct gatcaatgaa
      841 aacgttgacc ggaaagactt ttttgataac ggagagtggg agatactaaa tgcaaagggg
      901 atgaagggca acagaagaga aggcttttac tcctatccgt ttgttaccta ctcttttgtt
      961 ctgagacgcc tgcccctgtt ttacaccctc tttttgataa tcccctgcct ggggctgtct
     1021 tttctcacgg tcctggtgtt ctacctaccc tccgacgaag gggaaaaact ctccttatcc
     1081 acctcggttt tggtttcttt gacagtgttt cttttagtaa tcgaagaaat aatcccgtct
     1141 tcttcaaaag tcatccctct cattggggag tacctcctct tcattatgat atttgtcacg
     1201 ctgtctatta ttgtcacggt ttttgtaatt aatgtccacc acagatcttc ctcaacgtac
     1261 catcccatgg ccccctgggt gaagaggctg tttctggaaa aactcccgag atggctttgc
     1321 atgaaggacc ccagggaccg cttctctttc cccgatggaa cggagagtaa gggaaccgtg
     1381 agggggaaat ttccagggaa aaagaagcag actcctacca gtgatggaga aagagttctg
     1441 gtcgctttcc tggagaaggc ctctgagtcc atcagataca tctcgaggca tgtgaagaag
     1501 gaacacttca tcagtcaggt tgttcaagac tggaaattcg tggctcaggt tcttgaccgc
     1561 atcttcctct ggctctttct gacagcttcg gttttgggct ctgtcctgat ttttattcca
     1621 gccttgaaga tgtggataca tcgtttccat taggaactgc tctctggatc catgtagaag
     1681 acacacatag agagaatccc acctagaact gagggctgct ggtgtgttca cagaaaggag
     1741 ttgcatgcaa cagttgtggt gcccttgcct tgtcctggga gccttctgtg actggaggaa
     1801 actgagaaaa cgtgggtttg agctagtgtg atggtggctg ccattagaga tgtatttggg
     1861 caattcagag aagtctccat gtcatgttgt tgtgtgggag gctccgaaat gctccatttg
     1921 cctatccctg aactactgca tctgcccatc ccccagactt ctacatctgc tcatccctaa
     1981 actcttgatt ttgtccatcc actgaactcc tagtatctgc ccagcccctt atcgacttgt
     2041 ctgcacactg gggccatgtg gtacttctct agcagtgtgg ttgaacacgt tttgacaaca
     2101 gtttcaggaa ttacacaggt acaactcttg ccacacaggt caaagatgcc acttgtcaac
     2161 aaatgtccca taaatagggt cattgaagat gaccttgaat gactatgaca gttctctaag
     2221 gcaggggtta ttggaagttt gttcactgca ttgtaaactt ttctaataca ggtaggaact
     2281 acaccagaaa aatagccatt agctattaga actgcattca ttaatgcatg tctttttttt
     2341 ttttctctta gaaaattaaa agtgttaaat gttctttgat tatggttttg gctggtgtct
     2401 taggcagtgt tctattactg tgaagagaca ctgtaaccat ggcaacaggt caggtgcaga
     2461 ggtttagttc actttcatta cggtgggaaa catggtggca ctctggcaga tatggtgttg
     2521 ggagaggtag cctagagttt tacagcaaga gagagactgg tgtgggcttt tgaaacccca
     2581 aagcacaccc ccagtgacat acttccccca acaaatctac acctctggat ccttctcaag
     2641 gagtgacaat ccatgatgac taagtactaa agtatgggag ctcatggggg ccatttctat
     2701 tcaagccacc acagacagaa ccggttttca cagagttttc tgcctccacc tgtgtcccct
     2761 ctccctgtgt ctccctcctt tcctcatccc accccatgct cctccaggac agtcaggaag
     2821 tgtttatcag atttactttg ctgccgttgc ttctgagtcc ttcctgggct ttgttaaagg
     2881 agtttgttct tccacctcag tgaaacccca tgtgctctgt tttatttgag acacggttct
     2941 tctaagcagt ttatccctga gatttaaaaa accctaatct aagattttat ttttagatta
     3001 ctataactgc aacattcagt aagcgctgcg ttctcttatt cctactagtc tgattatttt
     3061 ttttcttcct aaatagctta agcttttctt atcttcagtc ttagacatgt cacactgtgc
     3121 ctgagcagag aatgataccc ccatccatct ctatctctat tccctccacc ctccccttct
     3181 cctctcctct ctgccatctc cttctctctg cacgcgatga ctagtgtctt caagctatgc
     3241 agattctttt ctatttctct tactatctct gttctgcatt ttctctcttt gtctctaaaa
     3301 atttctgata aaatttcttg tcattgttgg gcctgtactc cttgtttatt aactaaccgg
     3361 ttgactcttc tttcatactt ttaaaattct ttaattaata gtttcttaat tctttgacaa
     3421 ttccatacaa catattttga tcctatttac cccaaattcc tcccctgtaa cttctcctag
     3481 acccactccc tcaccctatc cccccacatc aggtccttat tatttatttt taaatgcccc
     3541 cctcccctga gtccaatttg tgttgcctgc atactccggt gtggtcccag cctttggtcc
     3601 caactctgca gctggttcct ctctagctgt atcagtctgt gtgagcaagg gagggtagga
     3661 gctcttgctg atagcacagc gggagactct gcgctcagac taccagcatc atgcttccaa
     3721 ccctggcaag cagcctgcat gaacccctca ctgcccatgg cggggtcagc ccttggtcag
     3781 acttctctaa ctagtctttt gtttggactc ttccaatggt gtttaagtgc ctttgtagcg
     3841 ggaatgctgt aaaccctgat ctggagttcc cactgagtcc aaccacagcc agtcgaagga
     3901 gaatctctgc ttcaggttct ttagatcgcc ttgcacagac tacacggctc tcccacctcg
     3961 ctgccaatag tgtgcccaca agatccagca cacgagatgg atttttaaag aaatggataa
     4021 acacagtgga acaagagcta ggtcacacag attgctggtc ctagctctgt ggctaaccag
     4081 tggtgtgagc agtgggcctt acacacgctg tctccctgag gactttttag cttgtgggtc
     4141 agcagaacga ggtgagcatt cgtgaccttt gaagttctcc tcagtcctgt actctatctt
     4201 ttggaggttt gctatggagc ttcctcagtg ttcaacccca tggtcctcac agtcgcattg
     4261 ttccttgctg ggcaggtagt gttgaagggt gtgtgtggtc tgctctgtgg ctccctgagg
     4321 gtagacattg tgataaaaat caaggcttag ctatgacttc ctgaatgata ctgtggataa
     4381 aagaacgaat agttattatt ccccctcctc caggttgatt ttaattcatc acatttgatg
     4441 tgtgattaca ctaggaaaag tgcagtatca atgttagaaa aaaaatgttt ggatctgagc
     4501 ttagctttac atttggaaaa ataccagcac gaaccatttc ccacaacaaa tattttctgt
     4561 tgaatcaaat aaagaaaaga ttaagcctaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4621 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4681 aaaaaaaaaa aa
//
