LOCUS       NM_001373901            6377 bp    mRNA    linear   ROD 05-OCT-2024
DEFINITION  Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 5
            (Chrna5), transcript variant 1, mRNA.
ACCESSION   NM_001373901 XM_006510773
VERSION     NM_001373901.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Tsuzuki,A., Yamasaki,M., Konno,K., Miyazaki,T., Takei,N.,
            Tomita,S., Yuzaki,M. and Watanabe,M.
  TITLE     Abundant extrasynaptic expression of alpha3beta4-containing
            nicotinic acetylcholine receptors in the medial
            habenula-interpeduncular nucleus pathway in mice
  JOURNAL   Sci Rep 14 (1), 14193 (2024)
   PUBMED   38902419
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 6377)
  AUTHORS   Yang,K., McLaughlin,I., Shaw,J.K., Quijano-Carde,N., Dani,J.A. and
            De Biasi,M.
  TITLE     CHRNA5 gene variation affects the response of VTA dopaminergic
            neurons during chronic nicotine exposure and withdrawal
  JOURNAL   Neuropharmacology 235, 109547 (2023)
   PUBMED   37116611
  REMARK    GeneRIF: CHRNA5 gene variation affects the response of VTA
            dopaminergic neurons during chronic nicotine exposure and
            withdrawal.
REFERENCE   4  (bases 1 to 6377)
  AUTHORS   Brynildsen,J.K., Yang,K., Lemchi,C., Dani,J.A., De Biasi,M. and
            Blendy,J.A.
  TITLE     A common SNP in Chrna5 enhances morphine reward in female mice
  JOURNAL   Neuropharmacology 218, 109218 (2022)
   PUBMED   35973602
  REMARK    GeneRIF: A common SNP in Chrna5 enhances morphine reward in female
            mice.
REFERENCE   5  (bases 1 to 6377)
  AUTHORS   Quijano Carde,N.A., Shaw,J., Carter,C., Kim,S., Stitzel,J.A.,
            Venkatesh,S.K., Ramchandani,V.A. and De Biasi,M.
  TITLE     Mutation of the alpha5 nicotinic acetylcholine receptor subunit
            increases ethanol and nicotine consumption in adolescence and
            impacts adult drug consumption
  JOURNAL   Neuropharmacology 216, 109170 (2022)
   PUBMED   35752273
  REMARK    GeneRIF: Mutation of the alpha5 nicotinic acetylcholine receptor
            subunit increases ethanol and nicotine consumption in adolescence
            and impacts adult drug consumption.
REFERENCE   6  (bases 1 to 6377)
  AUTHORS   Miner,L.L. and Marley,R.J.
  TITLE     Chromosomal mapping of the psychomotor stimulant effects of cocaine
            in BXD recombinant inbred mice
  JOURNAL   Psychopharmacology (Berl) 122 (3), 209-214 (1995)
   PUBMED   8748389
REFERENCE   7  (bases 1 to 6377)
  AUTHORS   Drescher,D.G., Khan,K.M., Green,G.E., Morley,B.J., Beisel,K.W.,
            Kaul,H., Gordon,D., Gupta,A.K., Drescher,M.J. and Barretto,R.L.
  TITLE     Analysis of nicotinic acetylcholine receptor subunits in the
            cochlea of the mouse
  JOURNAL   Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 112 (3),
            267-273 (1995)
   PUBMED   8838678
REFERENCE   8  (bases 1 to 6377)
  AUTHORS   Nagavarapu,U. and Boyd,R.T.
  TITLE     Distribution of neuronal nicotinic acetylcholine receptor gene
            restriction fragment length polymorphisms in inbred and partially
            inbred mice
  JOURNAL   Pharmacogenetics 5 (5), 326-331 (1995)
   PUBMED   8563774
REFERENCE   9  (bases 1 to 6377)
  AUTHORS   Anand,R. and Lindstrom,J.
  TITLE     Chromosomal localization of seven neuronal nicotinic acetylcholine
            receptor subunit genes in humans
  JOURNAL   Genomics 13 (4), 962-967 (1992)
   PUBMED   1505988
REFERENCE   10 (bases 1 to 6377)
  AUTHORS   Eng,C.M., Kozak,C.A., Beaudet,A.L. and Zoghbi,H.Y.
  TITLE     Mapping of multiple subunits of the neuronal nicotinic
            acetylcholine receptor to chromosome 15 in man and chromosome 9 in
            mouse
  JOURNAL   Genomics 9 (2), 278-282 (1991)
   PUBMED   2004777
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161264.2.
            
            On Oct 28, 2019 this sequence version replaced XM_006510773.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ318788.1, SRR11927933.2222185.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-234               AC161264.2         42246-42479         c
            235-386             AC161264.2         25804-25955         c
            387-431             AC161264.2         23471-23515         c
            432-541             AC161264.2         21458-21567         c
            542-1370            AC161264.2         18808-19636         c
            1371-6377           AC161264.2         12547-17553         c
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 29.84 cM"
     gene            1..6377
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /note="cholinergic receptor, nicotinic, alpha polypeptide
                     5"
                     /db_xref="GeneID:110835"
                     /db_xref="MGI:MGI:87889"
     exon            1..234
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /inference="alignment:Splign:2.1.0"
     CDS             129..1532
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; neuronal acetylcholine receptor subunit
                     alpha-5; nicotinic acetylcholine receptor alpha5 subunit"
                     /codon_start=1
                     /product="neuronal acetylcholine receptor subunit alpha-5
                     isoform 1 precursor"
                     /protein_id="NP_001360830.1"
                     /db_xref="GeneID:110835"
                     /db_xref="MGI:MGI:87889"
                     /translation="MAARGSRRRALRLLLMVQLLAGRWRPAGAARGARGGLPELSSAA
                     KHEDSLFRDLFEDYEKWVRPVEHLSDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQE
                     WIDVKLRWNPDDYGGIKIIRVPSDSLWIPDIVLFDNADGRFEGASTKTVVRYNGTVTW
                     TQPANYKSSCTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQDVDRTDFFDNGEW
                     EIMSAMGSKGNRTDSCCWYPCITYSFVIKRLPLFYTLFLIIPCIGLSFLTVVVFYLPS
                     NEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKVIPLIGEYLVFTMIFVTLSIMVTVFA
                     INIHHRSSSTHNAMAPWVRKIFLHKLPKLLCMRSHADRYFTQREEAEKDGGPKSRNTL
                     EAALDCIRYITRHVVKENDVREVVEDWKFIAQVLDRMFLWTFLLVSIIGTLGLFVPVI
                     YKWANIIVPVHIGNTIK"
     exon            235..386
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /inference="alignment:Splign:2.1.0"
     exon            387..431
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /inference="alignment:Splign:2.1.0"
     exon            432..541
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /inference="alignment:Splign:2.1.0"
     exon            542..1370
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..6377
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2740
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /note="major polyA site"
     regulatory      6355..6360
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /note="hexamer: AATAAA"
     polyA_site      6377
                     /gene="Chrna5"
                     /gene_synonym="Acra-5; Acra5"
                     /note="major polyA site"
ORIGIN      
        1 gtgcagaagc tgaagagcga gcggcgagcg gcttccgaga aacgagcgga tttcggcggc
       61 agctcttgtg ctccgtccct cagggcccgg agtaatccgc gaggcagccg tcggcgggcg
      121 gacgggcgat ggcggcgcgg gggtcgcggc ggcgcgcgct gcgcctgctc ttgatggtgc
      181 agctgctggc ggggcgctgg cggccggccg gggcggcgcg gggcgcgcgg ggagggttgc
      241 ctgagctatc ctctgctgca aaacatgaag acagtttgtt tagggattta tttgaagact
      301 atgaaaagtg ggttcgtcct gtggaacacc tgagtgacaa gataaaaatc aagtttggcc
      361 ttgcaatatc tcagttagtg gacgtggatg agaaaaacca gctaatgacc accaacgtct
      421 ggttgaagca ggaatggata gatgtaaagt taagatggaa tcctgatgac tatggtggaa
      481 taaaaattat acgtgttcct tcagactctc tgtggatccc agacatcgtt ttgtttgata
      541 atgcagacgg acgtttcgaa ggggccagta cgaaaacagt tgtcaggtac aatggcactg
      601 tcacttggac gcagccagca aactacaaaa gttcttgcac tatagatgtg acctttttcc
      661 catttgatct ccaaaattgc tccatgaaat tcggctcatg gacgtatgat ggatcccagg
      721 tcgatataat cctagaggac caagatgtcg acagaacaga cttttttgac aatggagaat
      781 gggaaatcat gagcgcaatg gggagcaagg ggaaccggac ggacagctgc tgctggtacc
      841 cctgcatcac ctactccttc gtgatcaaac ggctgcctct cttctacacc ctgtttctta
      901 tcataccctg catcgggctc tcgtttctga ctgtggttgt cttctatctc ccttcaaacg
      961 aaggtgaaaa gattagcctg tgcacttccg tgctggtctc tctgactgtc ttcctgctgg
     1021 tgattgagga aattataccg tcatcttcca aagtcatacc tctgatcggg gagtacttgg
     1081 tgttcaccat gatcttcgtg accctatcca ttatggtgac tgtctttgcc atcaacatcc
     1141 accaccgctc ttcttccaca cacaatgcta tggcgccgtg ggttcgtaag atatttctcc
     1201 acaagcttcc caaactgctc tgcatgagaa gtcatgccga taggtacttc actcagagag
     1261 aagaagccga gaaagacggt ggacctaaat ctcggaatac tttggaggcc gcgctcgatt
     1321 gcattcgcta catcacgaga cacgtagtga aagagaacga cgtccgcgag gttgttgaag
     1381 attggaaatt catagcccaa gttctcgatc ggatgtttct atggacgttt cttctggttt
     1441 caatcattgg gacgctaggg ctttttgttc ctgttattta taaatgggcc aatataatag
     1501 tcccagttca cattggaaac acaattaagt gaaaccaaga aattacactg gacttagtga
     1561 gcagtcaagc agctcttagg acacgtatgc cattatgaaa atatttacaa tttgatatag
     1621 gcagtaacag attagcagtt tctgacattg gcttaatgtt gtccattaga actgtagtaa
     1681 tagccttgag taccaacact gtccgcctgc actagtgaag gcctaccatc gcatcctggc
     1741 aaatcctact aacttgcaac cagtgacgaa gggcatcttt ggagtactgg aaaatgcaac
     1801 tatatttgaa gactgtttaa agcttttctc cccacattta gtaaaagcat atatttgtgt
     1861 ggttttgaat tttcagaatg ggtcttgtta aattgtccag gctggcccta aacctcctga
     1921 gtagctagga ctatgggtgt gctccatttt gccctgttct gtattcatgg atataaaaca
     1981 catcatactt ttataggaga taagccgatt acttggttta ataataacta tttttgtgaa
     2041 gagacaccat gatcaacaac tcttataaag gaaaacattt aattggggct ggcttactgg
     2101 ttctgaggtt tagtccatta ttgtcatggc agaagcatgg cagcatacag gcagacataa
     2161 tgttgaagac aagagttttc tacatctgga ttggctggca gcaagaagag agagtgaccc
     2221 actggacatg acttgagctt ctgaaacctc agaacccacc tcctgtgaca cactttcttc
     2281 aacaaggcca cacctcctca tagtgctact ccttggtgat caagcattca atctatgggt
     2341 ctatgagaac catttctatt cacaccaccc ttgataggat gctattctta ctgacatttt
     2401 taatatgtga aaaatggtaa ctagaaactc cttaagtcta ctttattctt catatggcaa
     2461 tatggaatgg cttttgtgct aactaacttt gcagcttact gtccactgaa aacatataac
     2521 aggcatctcc tgcagacatt ctttgtaatg actttataat cctgcagtga cacatttttt
     2581 gtaatgactt cagtcgcctt gtccatattc tttggttagg agagctactt atttatctct
     2641 gatttcagaa taatactgca gttgtacaga ttcccgtctc aaaacgggat gttatgtctc
     2701 tgtcttggta gttctgtgga aaaaatcatt ctgggctgga tatggtgtct catacctgtc
     2761 atctctactt tcaggaggca ggggcagggg aattgttgtg agttgtaggt catcctgggt
     2821 tacagtagga caccctgtct caaaagccaa acaaacaaaa attgcccttc tgagttgatg
     2881 taagcacact gtcacatctt ataatgtctc aatcatcttt caccccaatt ataatggtca
     2941 aatcttagag caaaacgctt tagatatagt ctagctagcc tattactagg tatgatatca
     3001 gtcagtactg tgtaatgtaa catgggtaaa gtagttaaac ttcatcccta gtgccccaac
     3061 tcagctataa cccaaaagct tgcgtttgat tcacaactct tagttgtaaa gttagcaaca
     3121 tctttggtta ttagtaatat attatcaaca atttaaattc aggtcagcat tctttgaaag
     3181 gcaggatgga ccctgtcaag tttaatttta ttgtgattat tgtgtttatg actgttagaa
     3241 tgagtgaaag tctgtactgt agtttagtaa atgtccctca aaggctcatt gttgaaagct
     3301 tggtcatggt ggttttggga ggtggtggga tcttcaggag tataggctct ggcaggggaa
     3361 agttaggtct ctaggagcgt gcccttgagg atattgagac cccagctctt ccttttgctt
     3421 cccagacacc atgaggtagg caacttggct ctgctttgtg tctcccagtg ttatatacat
     3481 cttgacactg acctcaaagc aagaccccat catggactaa ggcctctgca agcatgagca
     3541 acaacaaagc tttctcttaa ttagctcaag tattttcttg attatctcaa gtgtttttcc
     3601 cataatagca gaaagctaat agaatattag tatcctgaag tgaaatctct ggtgtgactt
     3661 ttgctgtgga gtctacttct agtcattcta gactttaaaa aggtaggaat agaacaaaga
     3721 gataattctg cccaagtaca gcttggaaac cagtgagttt actgtggcta cttataggaa
     3781 catggaggga ggggtttttc catggaagaa accatggaaa gcccacctta gcctgggtaa
     3841 caacttttac aacctgcagg taacagtcct gcagtttcct ctttcctgtt gtttgctatt
     3901 tttataaatt caggaatgaa tcttgggagt ctggtaagtc ctgtgagttt catgaatctg
     3961 gtgagcatcc ctctcccccc tagaagggaa tgtttcaacc tggaggaaac agccacacaa
     4021 aagggtagtt attgtgataa taaataatgg ggtagctctg aagcctttga attttacccg
     4081 tagaaagaat ctggaaaggt ttgaagaaat gggatagaga aagcacttat ttgtatgggg
     4141 acttaagacc agaccagaca tgctattacc aatgttctgg ctacccacca gaacttgagg
     4201 gtaagactct atttctgaat tatcacatac tttcaagtca tggaacatga aaaaatgaag
     4261 ctggtactga cttggaaggt tcatcccgac tggaccgctt tcatagtgtg gaaggtgcta
     4321 tgtatgcaac cagaggacaa aagcaatcac caactgactg tgaaccttat gaactatact
     4381 aatggctgtc tggcaagatt caccctctgg cccgatagca tgtgaacatc ccaagagtgc
     4441 ccaaccactt tctgattgag tctaaatcca tcacaagaca aagctcatac ctggcaccat
     4501 ccaatggcca agaacccatg actagacagg taatgggctc ttgggggaga acatattgct
     4561 actattctgc taagtggaca tactactaaa ctgacaccta atgacgaatt gtgttaccct
     4621 tagataaatg catctctcag ccacttggcc acggtgtaga gcaaaagcaa ctgtagaatg
     4681 ctcaggccta aatgagacac agtataccac accctttctt cccaaggctc agagaccact
     4741 tcagaagaca gtaaagagtg taagacccag agatgttgga tgactacaag gaatgtgttt
     4801 tctgaagagc agctacacat atgagctcat ggtggttgtg ataacatgca caagacctgt
     4861 gcaaggccaa gatatctgat acaattagat aaaatctcat cacaaagagc aaagctgggc
     4921 gtgaagccct gctcctagct gactgactgc tggcagttgc tagacactag agaaagagtt
     4981 atgtttctct aagagagtag ccctgggtaa gctgaccaca ttccaatggg aggtcatata
     5041 tccaagaatg cttgggcaac acaaattggt cttgataaat gtatttttta attaatcact
     5101 tttaaaggac acaaagttga atgagtcaag aagagaggat gtgtctggga agtggtaaga
     5161 ggaggggatg agtatgatca aaatatgtat gaaattctca atgagtaaaa atatatttta
     5221 aaggggaagt caagtgcttg gcaccaggtt aatacaatac tgtgaaggtg cctcaagaat
     5281 tattcagact tgtgcctgtg caagctcaaa gaattcagac atttttccta aatagaccca
     5341 ggacagcctc cttccataag gcaatctaga aaatgttgcc ctgaagctcc ttcctcatct
     5401 gcagatactc gggtaggcca tatatagctt gtgcaaacct ggccatcatc cacataatgc
     5461 cgattttaca gacatgcaaa gtgaaagagt tgtggagtcc tggagttttc cacaaagatt
     5521 tcagaggtta tcagctctca aactggcaat atgttggaaa caagagtgtg aagctgaatc
     5581 taaaatggag accccagaaa gcaaggggtg acaaactgtg gagtgcctgc tgaggagagc
     5641 tgtgtgaggt aagttcagcc agccctagag aggcacaggg gctgcatgca gtcagcgagt
     5701 ctgtaggagc aggactgcct gcgcccttga gatttcacat actgccactg tgggcgccag
     5761 aggccagatg tctgccctgc ttagttttgc tcttgctttg atctgatctt ttctacacat
     5821 ctgtgctcct ttgtggaata gaaatgttta cctagtgtca atgtatcctg ggcatttata
     5881 atctttttac agcttctctg ccgagtttga catggactat ggaacaattc tgaagcagtt
     5941 aagactttgg gaactcttgc aaaaaacaaa ttatgaaatg gatataagtc tttggaagcc
     6001 aggccacatt ctgagtaagt gccaaaggcc agtgtatcaa aggtttggtc accagcctac
     6061 tcggtgctac tcagaggtgt ggaacctcta agaagaaagg agcttggtca ttgggagtgc
     6121 gtccttcttg gaaaaacagg agcccagctt cttccccatt ctttctgctt ccaggctgct
     6181 ttgaggtgaa tgtcattgat gaatcagaca cttcttacta tgaagtattg tctcatcaca
     6241 agcccaaaag gaatagacca catgagtggc tgtggactga aaccatgagc ccaaatacac
     6301 atttcctcct ttaaaattta ttatctcagg tgcttaacaa tatgaggaaa cactaataaa
     6361 aactagttta atgtaaa
//
