LOCUS       NM_015730               4575 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus cholinergic receptor, nicotinic, alpha polypeptide 4
            (Chrna4), transcript variant 1, mRNA.
ACCESSION   NM_015730
VERSION     NM_015730.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Henderson,B.J., Young,L.E., Olszewski,N.A., Tetteh-Quarshie,S.,
            Maddox,S.K., Simpkins,M.A., Dudich,M.C., McGlauglin,M.S.,
            Weinsweig,Z.C. and Cooper,S.Y.
  TITLE     Age-dependent effects of vaping on the prefrontal cortex, ventral
            tegmental area, and nucleus accumbens
  JOURNAL   Commun Biol 7 (1), 1553 (2024)
   PUBMED   39572675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Vilella,A., Romoli,B., Bodria,M., Pons,S., Maskos,U. and Zoli,M.
  TITLE     Evidence for a protective effect of the loss of alpha4-containing
            nicotinic acetylcholine receptors on Abeta-related neuropathology
            in Tg2576 mice
  JOURNAL   Front Neurosci 17, 1097857 (2023)
   PUBMED   37113156
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4575)
  AUTHORS   Bsharat,S., Monni,E., Singh,T., Johansson,J.K., Achanta,K.,
            Bertonnier-Brouty,L., Schmidt-Christensen,A., Holmberg,D.,
            Kokaia,Z., Prasad,R.B. and Artner,I.
  TITLE     MafB-dependent neurotransmitter signaling promotes beta cell
            migration in the developing pancreas
  JOURNAL   Development 150 (6) (2023)
   PUBMED   36897571
REFERENCE   4  (bases 1 to 4575)
  AUTHORS   Cooper,S.Y., Willis,C.V., Richardson,M.R., Hill,S.P., Wright,S.K.,
            Elmore,M.B., Mitchell,Z.B., Gonzalez Castro,A.K. and Henderson,B.J.
  TITLE     Chemical Flavorants in Vaping Products Alter Neurobiology in a
            Sex-Dependent Manner to Promote Vaping-Related Behaviors
  JOURNAL   J Neurosci 43 (8), 1360-1374 (2023)
   PUBMED   36690450
REFERENCE   5  (bases 1 to 4575)
  AUTHORS   Watson,W.H., Ritzenthaler,J.D., Torres-Gonzalez,E., Arteel,G.E. and
            Roman,J.
  TITLE     Mice lacking alpha4 nicotinic acetylcholine receptors are protected
            against alcohol-associated liver injury
  JOURNAL   Alcohol Clin Exp Res 46 (8), 1371-1383 (2022)
   PUBMED   35723023
  REMARK    GeneRIF: Mice lacking alpha4 nicotinic acetylcholine receptors are
            protected against alcohol-associated liver injury.
REFERENCE   6  (bases 1 to 4575)
  AUTHORS   Williamson,C.M., Dutton,E.R., Abbott,C.M., Beechey,C.V., Ball,S.T.
            and Peters,J.
  TITLE     Thirteen genes (Cebpb, E2f1, Tcf4, Cyp24, Pck1, Acra4, Edn3, Kcnb1,
            Mc3r, Ntsr, Cd40, Plcg1 and Rcad) that probably lie in the distal
            imprinting region of mouse chromosome 2 are not monoallelically
            expressed
  JOURNAL   Genet Res 65 (2), 83-93 (1995)
   PUBMED   7781998
REFERENCE   7  (bases 1 to 4575)
  AUTHORS   Pilz,A.J., Willer,E., Povey,S. and Abbott,C.M.
  TITLE     The genes coding for phosphoenolpyruvate carboxykinase-1 (PCK1) and
            neuronal nicotinic acetylcholine receptor alpha 4 subunit (CHRNA4)
            map to human chromosome 20, extending the known region of homology
            with mouse chromosome 2
  JOURNAL   Ann Hum Genet 56 (4), 289-293 (1992)
   PUBMED   1492743
REFERENCE   8  (bases 1 to 4575)
  AUTHORS   Anand,R. and Lindstrom,J.
  TITLE     Chromosomal localization of seven neuronal nicotinic acetylcholine
            receptor subunit genes in humans
  JOURNAL   Genomics 13 (4), 962-967 (1992)
   PUBMED   1505988
REFERENCE   9  (bases 1 to 4575)
  AUTHORS   Pilz,A., Le Tissier,P., Moseley,H., Peters,J. and Abbott,C.
  TITLE     Mapping of the structural gene for S-adenosyl homocysteine
            hydrolase to mouse chromosome 2, and related sequences to
            chromosomes 8 and X
  JOURNAL   Mamm Genome 3 (11), 633-636 (1992)
   PUBMED   1450513
REFERENCE   10 (bases 1 to 4575)
  AUTHORS   Bessis,A., Simon-Chazottes,D., Devillers-Thiery,A., Guenet,J.L. and
            Changeux,J.P.
  TITLE     Chromosomal localization of the mouse genes coding for alpha 2,
            alpha 3, alpha 4 and beta 2 subunits of neuronal nicotinic
            acetylcholine receptor
  JOURNAL   FEBS Lett 264 (1), 48-52 (1990)
   PUBMED   2338144
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL450341.10.
            
            On Feb 21, 2023 this sequence version replaced NM_015730.5.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053013.1, AK143938.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-324               AL450341.10        38129-38452         c
            325-476             AL450341.10        36556-36707         c
            477-521             AL450341.10        33246-33290         c
            522-631             AL450341.10        33057-33166         c
            632-2006            AL450341.10        27335-28709         c
            2007-4575           AL450341.10        21448-24016         c
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 103.54 cM"
     gene            1..4575
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /note="cholinergic receptor, nicotinic, alpha polypeptide
                     4"
                     /db_xref="GeneID:11438"
                     /db_xref="MGI:MGI:87888"
     exon            1..324
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /inference="alignment:Splign:2.1.0"
     exon            325..476
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /inference="alignment:Splign:2.1.0"
     exon            477..521
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /inference="alignment:Splign:2.1.0"
     exon            522..631
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /inference="alignment:Splign:2.1.0"
     exon            632..2006
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..4575
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4547..4552
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /note="hexamer: AATAAA"
     polyA_site      4575
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /note="major polyA site"
ORIGIN      
        1 agtccctccc cctcctcccc tctcgccggc ggctggaggc tgcaggtccg ggcggggccg
       61 cgccgcggag cccacaacct cgagctcccg ccccgccgcc gcaccccgcg ggacgagctg
      121 cggagcccgg tttggggacg agcggccgcg acacggggca tgaagttggg tgcgcgcggg
      181 tctcggagcg gaggcgcggt actgccggga gccgccctcg tctagagccc gttctgtgag
      241 ccatggagat cgggggctcc ggggcgccgc cgccgctgct gctcctgccg ctcctgctgc
      301 tcttagggac cggcctcttg cctgctagca gccacataga gacccgggcc catgcggagg
      361 agcggctcct gaagagactc ttctctggct acaacaagtg gtctcggcca gtagccaata
      421 tctcagatgt ggtccttgtc cgctttggct tgtcgattgc tcagctcatt gatgtggatg
      481 agaaaaacca gatgatgacg accaacgtgt gggtgaagca ggagtggcat gactacaaac
      541 tgcgctggga ccctggtgac tacgagaatg tcacctccat ccgcatccca tctgaactca
      601 tctggaggcc tgacatcgtc ctctacaaca acgcggacgg ggactttgca gtcacccacc
      661 taaccaaagc ccacctgttc tatgatgggc gtgtgcagtg gacacccccg gccatctata
      721 agagctcctg cagcatcgac gtcaccttct tccccttcga ccagcagaac tgtaccatga
      781 agtttgggtc ctggacctac gacaaggcca agattgactt ggtgagcatg cacagccgtg
      841 tggaccaact ggacttctgg gaaagtgggg agtgggtcat tgtggatgcc gtgggcacct
      901 acaacaccag gaagtatgaa tgctgtgccg agatctatcc tgacatcacc tacgccttca
      961 tcatccgccg actgccactg ttctacacca tcaaccttat catcccgtgc ctgctcatct
     1021 cctgcctcac cgtgctggtc ttctatctgc cctcggagtg cggcgagaag gtcacgctgt
     1081 gcatctcggt gctgctttct ctcaccgtct tcctgctgct catcaccgag atcatcccgt
     1141 ccacctcgct ggtcatcccg ctcatcggcg agtacctgct cttcaccatg atcttcgtca
     1201 ccctctccat tgtcatcacg gtcttcgtgc tcaatgtaca ccaccgctca ccacgcacac
     1261 acaccatgcc cgcctgggtg cgcagagtct tcctggacat tgtgccccgt ctcctcttca
     1321 tgaagcgccc atctgtggtc aaagacaact gccggagact tatcgaatcc atgcacaaga
     1381 tggccaacgc ccctcgtttc tggccagagc ctgagagtga gcccggcatc ttgggtgaca
     1441 tctgcaacca aggcctgtca cctgccccaa ctttctgcaa ccgcatggac acagcagtcg
     1501 agacccagcc tacatgcagg tcaccctccc acaaggtccc tgacttgaag acatcagagg
     1561 ttgagaaggc cagtccctgt ccatcacctg gctcttgtca cccacccaat agcagtgggg
     1621 ccccagtgct catcaaagcc aggtccctga gcgtccagca tgtgcccagc tcccaggaag
     1681 cagccgaggg cagcatccgc tgccggtctc ggagtatcca gtactgtgtt tcccaagatg
     1741 gagctgcttc cctgactgag agcaagccca ctggctcccc agcctccctg aagacccgtc
     1801 catcccagct tccagtgtca gaccagacct ctccatgcaa atgcacatgc aaggaaccat
     1861 ctcctgtgtc ccccatcact gtgctcaagg ctggaggcac caaagcacct ccccaacacc
     1921 tgcccctgtc accagccctg acacgggcag tagaaggcgt ccagtacatt gcagaccacc
     1981 tcaaggcaga agacacagac ttctcggtga aggaggactg gaaatacgtg gccatggtca
     2041 ttgaccgaat cttcctctgg atgttcatca ttgtctgcct tctgggcact gtgggactct
     2101 tcctgcctcc atggttggct ggtatgatct agggaatagc ggcacctagc tcccaggtct
     2161 ctacagggcc atgcgactcg tcagtcaccc acatcttcca aaccggccat gagacaccta
     2221 ggagagagag atgctgccct ggttgaccct ggttctagtc aggccacagg cctggttgga
     2281 gctagttgag gactgatata gttacaggct gagtccctca ttaaagtttc tccagagcaa
     2341 gtgacagtca ctccctggct tacagacagc acacacccat ctgtgtcaca gagaatgatc
     2401 cagtgttgat ctcagttgtc ctttgaggcc aaaacaattc atcccccttc aggaaccaga
     2461 gcccctcgtg ctgtgggatt cctacggccc aggaaattcc catggtgctc tgctggccac
     2521 accctctccc tccccataat gtggttccct caaccctcca ggctgggctg ctctctgact
     2581 caaaggtgtc agatgtaagc cccggcaggt ttttattttg tttaggttga agcgaattgg
     2641 taagaaatag agcagtgaga tatgtggatg agtcccactc acaggtgaat gagtgcaggg
     2701 tctcacagga agagtgaggc accacaggac tcctgcttcc atctcagggt cacaggcatc
     2761 aatcatgagc atttcctagg gtccataaac ccgaggaggg caagggcata gagggtctca
     2821 gggttgtgat ggagccaaat cctgtccagg gcctgggccg ttcatcccct catggatctt
     2881 ccttgatatc cctgtatgtt tctgcctctc tggaattaga agactgaaag taagatttct
     2941 catcacggtc ctgtggtgtg gccacagttc acctgagcac atctctctag accagtagga
     3001 gtggtgcgaa gccccttcaa tgttgtagaa tagcgtgagc tgccaagaga cttctaagca
     3061 aaacaggctc tgtgactcat ttttcgaggg ccatcgacca agtcttaggg gtgcctcacc
     3121 ctgtctgcct tgcacttagg gaagacccga gaggttcctc ttccccttcc caagatggca
     3181 ccaggcaacc tagagaaccc accgtggtgg gatgggagaa cgaacatgct gtgcacatct
     3241 ctatgagatt ccactgagtg aagcccagaa catgggaggc atgcaagaaa tcaccctgtg
     3301 tcgtggtccc agttgaccct ccgctgtctc caccagccag gtgggtttca cagagctggg
     3361 ccctgcaccc tcagccaagc tgttctaggc cctgaagctg aggtccctgt ttggatagtc
     3421 ctggggactg cagaatgaaa gaagaactta atgaacgcac caagcctcca gtaggtacgg
     3481 ctgccacctc cgtggtatga cttgcccatc ccagctgaat gaggatgtca ggaaggaggt
     3541 atgccagagg gccagcattg cctttacctg actacctaca ggcaaatcca cctttaaaca
     3601 cagagctgct ggacatccag ggtgctggtg ggaaaggaac tccacactgg gagccccagg
     3661 ccattcctat gaacaggaag gggatgcaga ggcctggtct ctgaactctg gatattgttc
     3721 caggtcttcc ctagagtcct aagggcatcg aggatcccat ctgccatgtt tcagtctgcc
     3781 ctccactgac tgactagatc tctagcccct atattggaac tgtcgggatg ctgcaagatg
     3841 accctggtgg ggaaattcat gccagaatct ggggaccaag gggaacacaa gccccagtga
     3901 tgaagacagc aggtaacacc tgacagatgt gtgttctacc attatggtgc atacgtggct
     3961 ccaaccacaa gaaatgcaga caacagtgga gatcagggca aggccattgt gacatggaac
     4021 aggaccgctg tgctggtctc ttcagggtta ggaaaactga actgctggga tgctcctgac
     4081 aggctaccca cttcccccta ccccccaaca cacattcaca agccagaaaa ggaaaataaa
     4141 acaccgtgtt ctccccattc ccactcagcc ggccttttgt ctgcctgctt ccagtgttga
     4201 tatgtgttca agataaagtt cagttagggc agaatgcttg atttaagact tttgaaccag
     4261 tgagctttaa agaacagaga ctgtgtggcc ccagcccctc tgatacgtag acattatctc
     4321 cccaaagctc ccagtcctcc cagtctaccc catcccatta gacagcatca actcaaatgt
     4381 gagtcttgga gaccagttcg gctagccatc atatgtctgg gaatcccata ttggactctg
     4441 caatgtctgg cttctttcgc ttgtgtggcc aaggctcatc tgcggtgttg tgtgtgatag
     4501 actcattgct gttgtgtgct tgttggatct tagttgtttc tgtctgaata aaccgagtcg
     4561 tggtgtcttc cccca
//
