LOCUS       NP_056545                629 aa            linear   ROD 17-DEC-2024
DEFINITION  neuronal acetylcholine receptor subunit alpha-4 isoform 1 precursor
            [Mus musculus].
ACCESSION   NP_056545
VERSION     NP_056545.3
DBSOURCE    REFSEQ: accession NM_015730.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 629)
  AUTHORS   Henderson,B.J., Young,L.E., Olszewski,N.A., Tetteh-Quarshie,S.,
            Maddox,S.K., Simpkins,M.A., Dudich,M.C., McGlauglin,M.S.,
            Weinsweig,Z.C. and Cooper,S.Y.
  TITLE     Age-dependent effects of vaping on the prefrontal cortex, ventral
            tegmental area, and nucleus accumbens
  JOURNAL   Commun Biol 7 (1), 1553 (2024)
   PUBMED   39572675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 629)
  AUTHORS   Vilella,A., Romoli,B., Bodria,M., Pons,S., Maskos,U. and Zoli,M.
  TITLE     Evidence for a protective effect of the loss of alpha4-containing
            nicotinic acetylcholine receptors on Abeta-related neuropathology
            in Tg2576 mice
  JOURNAL   Front Neurosci 17, 1097857 (2023)
   PUBMED   37113156
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 629)
  AUTHORS   Bsharat,S., Monni,E., Singh,T., Johansson,J.K., Achanta,K.,
            Bertonnier-Brouty,L., Schmidt-Christensen,A., Holmberg,D.,
            Kokaia,Z., Prasad,R.B. and Artner,I.
  TITLE     MafB-dependent neurotransmitter signaling promotes beta cell
            migration in the developing pancreas
  JOURNAL   Development 150 (6) (2023)
   PUBMED   36897571
REFERENCE   4  (residues 1 to 629)
  AUTHORS   Cooper,S.Y., Willis,C.V., Richardson,M.R., Hill,S.P., Wright,S.K.,
            Elmore,M.B., Mitchell,Z.B., Gonzalez Castro,A.K. and Henderson,B.J.
  TITLE     Chemical Flavorants in Vaping Products Alter Neurobiology in a
            Sex-Dependent Manner to Promote Vaping-Related Behaviors
  JOURNAL   J Neurosci 43 (8), 1360-1374 (2023)
   PUBMED   36690450
REFERENCE   5  (residues 1 to 629)
  AUTHORS   Watson,W.H., Ritzenthaler,J.D., Torres-Gonzalez,E., Arteel,G.E. and
            Roman,J.
  TITLE     Mice lacking alpha4 nicotinic acetylcholine receptors are protected
            against alcohol-associated liver injury
  JOURNAL   Alcohol Clin Exp Res 46 (8), 1371-1383 (2022)
   PUBMED   35723023
  REMARK    GeneRIF: Mice lacking alpha4 nicotinic acetylcholine receptors are
            protected against alcohol-associated liver injury.
REFERENCE   6  (residues 1 to 629)
  AUTHORS   Williamson,C.M., Dutton,E.R., Abbott,C.M., Beechey,C.V., Ball,S.T.
            and Peters,J.
  TITLE     Thirteen genes (Cebpb, E2f1, Tcf4, Cyp24, Pck1, Acra4, Edn3, Kcnb1,
            Mc3r, Ntsr, Cd40, Plcg1 and Rcad) that probably lie in the distal
            imprinting region of mouse chromosome 2 are not monoallelically
            expressed
  JOURNAL   Genet Res 65 (2), 83-93 (1995)
   PUBMED   7781998
REFERENCE   7  (residues 1 to 629)
  AUTHORS   Pilz,A.J., Willer,E., Povey,S. and Abbott,C.M.
  TITLE     The genes coding for phosphoenolpyruvate carboxykinase-1 (PCK1) and
            neuronal nicotinic acetylcholine receptor alpha 4 subunit (CHRNA4)
            map to human chromosome 20, extending the known region of homology
            with mouse chromosome 2
  JOURNAL   Ann Hum Genet 56 (4), 289-293 (1992)
   PUBMED   1492743
REFERENCE   8  (residues 1 to 629)
  AUTHORS   Anand,R. and Lindstrom,J.
  TITLE     Chromosomal localization of seven neuronal nicotinic acetylcholine
            receptor subunit genes in humans
  JOURNAL   Genomics 13 (4), 962-967 (1992)
   PUBMED   1505988
REFERENCE   9  (residues 1 to 629)
  AUTHORS   Pilz,A., Le Tissier,P., Moseley,H., Peters,J. and Abbott,C.
  TITLE     Mapping of the structural gene for S-adenosyl homocysteine
            hydrolase to mouse chromosome 2, and related sequences to
            chromosomes 8 and X
  JOURNAL   Mamm Genome 3 (11), 633-636 (1992)
   PUBMED   1450513
REFERENCE   10 (residues 1 to 629)
  AUTHORS   Bessis,A., Simon-Chazottes,D., Devillers-Thiery,A., Guenet,J.L. and
            Changeux,J.P.
  TITLE     Chromosomal localization of the mouse genes coding for alpha 2,
            alpha 3, alpha 4 and beta 2 subunits of neuronal nicotinic
            acetylcholine receptor
  JOURNAL   FEBS Lett 264 (1), 48-52 (1990)
   PUBMED   2338144
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL450341.10.
            
            On Jun 9, 2003 this sequence version replaced NP_056545.2.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053013.1, AK143938.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 103.54 cM"
     Protein         1..629
                     /product="neuronal acetylcholine receptor subunit alpha-4
                     isoform 1 precursor"
                     /note="neuronal acetylcholine receptor subunit alpha-4;
                     acetylcholine receptor, neuronal, nicotinic, alpha-4
                     subunit; acetylcholine receptor alpha 4 neural; a4
                     nicotinic receptor; alpha4 nAChR"
                     /calculated_mol_wt=67412
     sig_peptide     1..30
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2912
     mat_peptide     31..629
                     /product="Neuronal acetylcholine receptor subunit alpha-4.
                     /id=PRO_0000000352"
                     /note="propagated from UniProtKB/Swiss-Prot (O70174.2)"
                     /calculated_mol_wt=67412
     Region          39..245
                     /region_name="LGIC_ECD"
                     /note="extracellular domain (ECD) of Cys-loop
                     neurotransmitter-gated ion channels (also known as
                     ligand-gated ion channel (LGIC)); cl28912"
                     /db_xref="CDD:475126"
     Site            59
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O70174.2)"
     Site            order(74,76,82..83,90,112,114,123..124,126,128..129,
                     140..142,144,154,156,158,162,184,244)
                     /site_type="other"
                     /note="pentamer interface [polypeptide binding]"
                     /db_xref="CDD:349787"
     Site            109
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O70174.2)"
     Site            176
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O70174.2)"
     Site            250..270
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O70174.2)"
     Region          252..620
                     /region_name="Neur_chan_memb"
                     /note="Neurotransmitter-gated ion-channel transmembrane
                     region; pfam02932"
                     /db_xref="CDD:460753"
     Site            279..299
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O70174.2)"
     Site            312..332
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O70174.2)"
     Region          420..459
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O70174.2)"
     Site            427
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P09483; propagated from
                     UniProtKB/Swiss-Prot (O70174.2)"
     Region          503..529
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O70174.2)"
     Site            540
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (O70174.2)"
     Site            543
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (O70174.2)"
     Site            604..624
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O70174.2)"
     CDS             1..629
                     /gene="Chrna4"
                     /gene_synonym="Acra-4; Acra4; EBN1; ENFL1"
                     /coded_by="NM_015730.6:243..2132"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS17192.1"
                     /db_xref="GeneID:11438"
                     /db_xref="MGI:MGI:87888"
ORIGIN      
        1 meiggsgapp pllllpllll lgtgllpass hietrahaee rllkrlfsgy nkwsrpvani
       61 sdvvlvrfgl siaqlidvde knqmmttnvw vkqewhdykl rwdpgdyenv tsiripseli
      121 wrpdivlynn adgdfavthl tkahlfydgr vqwtppaiyk sscsidvtff pfdqqnctmk
      181 fgswtydkak idlvsmhsrv dqldfwesge wvivdavgty ntrkyeccae iypdityafi
      241 irrlplfyti nliipcllis cltvlvfylp secgekvtlc isvllsltvf llliteiips
      301 tslviplige yllftmifvt lsivitvfvl nvhhrsprth tmpawvrrvf ldivprllfm
      361 krpsvvkdnc rrliesmhkm anaprfwpep esepgilgdi cnqglspapt fcnrmdtave
      421 tqptcrspsh kvpdlktsev ekaspcpspg schppnssga pvlikarsls vqhvpssqea
      481 aegsircrsr siqycvsqdg aaslteskpt gspaslktrp sqlpvsdqts pckctckeps
      541 pvspitvlka ggtkappqhl plspaltrav egvqyiadhl kaedtdfsvk edwkyvamvi
      601 driflwmfii vcllgtvglf lppwlagmi
//
