LOCUS       NM_007699               2777 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), transcript
            variant 1, mRNA.
ACCESSION   NM_007699
VERSION     NM_007699.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Berezovskaia,A., Thomsen,M., Fink-Jensen,A. and Wortwein,G.
  TITLE     A sex-specific effect of M4 muscarinic cholinergic autoreceptor
            deletion on locomotor stimulation by cocaine and scopolamine
  JOURNAL   Front Mol Neurosci 17, 1451010 (2024)
   PUBMED   39737113
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2777)
  AUTHORS   Song,S., Lin,Z., Zhao,C., Wen,J., Chen,J., Xie,S., Qi,H., Wang,J.
            and Su,X.
  TITLE     Vagal-mAChR4 signaling promotes Friend virus complex (FV)-induced
            acute erythroleukemia
  JOURNAL   Virol Sin 38 (3), 429-439 (2023)
   PUBMED   37172825
  REMARK    GeneRIF: Vagal-mAChR4 signaling promotes Friend virus complex
            (FV)-induced acute erythroleukemia.
REFERENCE   4  (bases 1 to 2777)
  AUTHORS   Wang,N., Langfelder,P., Stricos,M., Ramanathan,L., Richman,J.B.,
            Vaca,R., Plascencia,M., Gu,X., Zhang,S., Tamai,T.K., Zhang,L.,
            Gao,F., Ouk,K., Lu,X., Ivanov,L.V., Vogt,T.F., Lu,Q.R.,
            Morton,A.J., Colwell,C.S., Aaronson,J.S., Rosinski,J., Horvath,S.
            and Yang,X.W.
  TITLE     Mapping brain gene coexpression in daytime transcriptomes unveils
            diurnal molecular networks and deciphers perturbation gene
            signatures
  JOURNAL   Neuron 110 (20), 3318-3338 (2022)
   PUBMED   36265442
REFERENCE   5  (bases 1 to 2777)
  AUTHORS   Shindo,Y., Nakamura,H.M., Nakai,J., Wakamori,M. and Nakamura,T.
  TITLE     A parasympathetic neurotransmitter induces myoepithelial cell
            differentiation during salivary gland development
  JOURNAL   Exp Cell Res 416 (1), 113137 (2022)
   PUBMED   35427599
REFERENCE   6  (bases 1 to 2777)
  AUTHORS   Forrester,L.M., Nagy,A., Sam,M., Watt,A., Stevenson,L.,
            Bernstein,A., Joyner,A.L. and Wurst,W.
  TITLE     An induction gene trap screen in embryonic stem cells:
            Identification of genes that respond to retinoic acid in vitro
  JOURNAL   Proc Natl Acad Sci U S A 93 (4), 1677-1682 (1996)
   PUBMED   8643689
REFERENCE   7  (bases 1 to 2777)
  AUTHORS   Lammerding-Koppel,M., Greiner-Schroder,A. and Drews,U.
  TITLE     Muscarinic receptors in the prenatal mouse embryo. Comparison of
            M35-immunohistochemistry with [3H]quinuclidinyl benzylate
            autoradiography
  JOURNAL   Histochem Cell Biol 103 (4), 301-310 (1995)
   PUBMED   7648406
REFERENCE   8  (bases 1 to 2777)
  AUTHORS   Andre,C., Dos Santos,G. and Koulakoff,A.
  TITLE     Cultured neurons from mouse brain reproduce the muscarinic receptor
            profile of their tissue of origin
  JOURNAL   Eur J Neurosci 6 (11), 1691-1701 (1994)
   PUBMED   7874308
REFERENCE   9  (bases 1 to 2777)
  AUTHORS   Van Koppen,C.J., Sell,A., Lenz,W. and Jakobs,K.H.
  TITLE     Deletion analysis of the m4 muscarinic acetylcholine receptor.
            Molecular determinants for activation of but not coupling to the Gi
            guanine-nucleotide-binding regulatory protein regulate receptor
            internalization
  JOURNAL   Eur J Biochem 222 (2), 525-531 (1994)
   PUBMED   8020490
REFERENCE   10 (bases 1 to 2777)
  AUTHORS   van Koppen,C.J., Lenz,W. and Nathanson,N.M.
  TITLE     Isolation, sequence and functional expression of the mouse m4
            muscarinic acetylcholine receptor gene
  JOURNAL   Biochim Biophys Acta 1173 (3), 342-344 (1993)
   PUBMED   7916637
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731772.9.
            
            On Feb 22, 2023 this sequence version replaced NM_007699.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.2769224.1,
                                           SRR17784646.848681.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-158               AL731772.9         59797-59954
            159-2777            AL731772.9         64828-67446
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 50.63 cM"
     gene            1..2777
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /note="cholinergic receptor, muscarinic 4"
                     /db_xref="GeneID:12672"
                     /db_xref="MGI:MGI:88399"
     exon            1..158
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /inference="alignment:Splign:2.1.0"
     exon            159..2777
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..1630
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /note="muscarinic acetylcholine receptor 4"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M4"
                     /protein_id="NP_031725.1"
                     /db_xref="CCDS:CCDS16439.1"
                     /db_xref="GeneID:12672"
                     /db_xref="MGI:MGI:88399"
                     /translation="MANFTPVNGSSANQSVRLVTTAHNHLETVEMVFIATVTGSLSLV
                     TVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTLYIIKGYWPLGAV
                     VCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSF
                     VLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHIS
                     LASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKPSIKKPPPGGASREELRNGKLEEAPP
                     PALPPPPRPVADKDTSNESSSGSATQNTKERPPTELSTTEAATTPALPAPTLQPRTLN
                     PASKWSKIQIVTKQTGSECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMA
                     ARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPERVWSIGYWLCYVNSTINP
                     ACYALCNATFKKTFRHLLLCQYRNIGTAR"
     regulatory      2749..2754
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /note="hexamer: ATTAAA"
     polyA_site      2777
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /note="major polyA site"
ORIGIN      
        1 gccacagcgc cgggagctgc aggcgaagca ggggccgccc cttggcaccc tgtccccgcc
       61 cccggccccc ggcccagtcg cccaccccac cgactcgcag ggcctctggc tagttccgcc
      121 gtctgtccgg cgccagcgga gcaagacaga agcagcggcc actgcccacc tgtctccagt
      181 tgggttcagc atggcgaact tcacacctgt caatggcagc tcagccaatc agtctgtgcg
      241 cctggtcaca acagcccaca accacctgga gacagtggag atggtgttca ttgcgacagt
      301 gactggttcc ctgagcctgg tgactgtggt gggtaacatc ctggtgatgc tgtccatcaa
      361 ggtcaacagg cagttgcaga cagtcaacaa ctacttcctg ttcagcctgg cgtgtgcaga
      421 tctcatcata ggggcgttct ctatgaacct ttacacctta tacatcatca agggctactg
      481 gcccctgggt gccgtggtct gtgacctgtg gctggccctg gactatgtgg tgagcaatgc
      541 ctctgtcatg aaccttctca tcatcagctt tgaccgctat ttctgcgtca ccaagcccct
      601 cacctatcca gcccgccgca ctactaagat ggcaggcctc atgattgcag ccgcctgggt
      661 cttgtccttt gtactctggg cccctgccat cttgttctgg cagtttgtgg tgggcaagag
      721 gacagtgcct gataaccagt gcttcatcca gttcttgtcc aacccggcgg tgaccttcgg
      781 cacagccatt gctgccttct acctgcctgt ggtcatcatg acggtgctgt atattcatat
      841 ctcgctggcc agccgcagcc gtgttcacaa gcatcgaccc gagggcccca aggagaagaa
      901 ggccaagact ctggctttcc tcaagagccc tctgatgaag ccgagcatta agaaacctcc
      961 accagggggc gcttctcgag aggaactgcg caacgggaag ctagaagagg ctcctccgcc
     1021 agccctgccc ccgcctccac gcccagtggc tgacaaggac acttccaatg agtccagctc
     1081 aggcagtgcc acccagaaca ccaaggaacg gccacccaca gagctgtcca ccacagaggc
     1141 cgccaccaca ccagcgctgc ccgctcctac cctgcagcca cgaaccctca acccagcctc
     1201 caagtggtcc aagatccaaa ttgtgacaaa gcagacaggc agtgaatgtg tgactgccat
     1261 cgagatcgta cctgccacgc cagctggtat gcgcccagca gccaatgtgg cccgaaagtt
     1321 tgccagcatc gctcgtaacc aggtgcgcaa gaagcggcag atggcggccc gggagcgcaa
     1381 agtgactcgg acaatctttg ccattctgct ggccttcatc ctcacctgga caccctacaa
     1441 tgtcatggtc ctggtgaaca ccttttgcca gagctgtatc cccgaaaggg tgtggtccat
     1501 cggctactgg ctctgctacg tcaacagcac gatcaaccct gcctgctatg cactctgcaa
     1561 tgccactttc aaaaagacct tccggcacct tttgctgtgc cagtatcgga acatcggcac
     1621 agccaggtag acgggcaggt gccttaggag gtgctggtgt caagagtgtg taccggggaa
     1681 ccacatggct caccggctgt ggaggagaga gtaggcggcc ccactctgag ttcgcatctg
     1741 ctgaagagaa caggtctccc agacaccgtg aggtacaaga ggctcttcgc tgggatggga
     1801 gagcagatct ctactgaagc ctaggaggct cggcccaggg acggtctgaa ctggcaccac
     1861 ctggcccata tcttttacct tgcctcagga agatggggga cgatggctgg agtgggcagt
     1921 ggctctatca aggccaacca tcggtggagg tggatgaata gagcagaact tgggagcatc
     1981 ttgagaggag caaaaggagc caggcttgca agggtggggt cccccgttat gctgtaatcg
     2041 gtgctttccg ccttcctgcg ccctcctctc ccttgcatgt aggctcagcc cttcccctgc
     2101 ccccaagtcc cactccaagg tgagggactt ccctgccact gttgctcagc caggctctgg
     2161 atgggaagcc aagccctttg gcactcccct ggccagcagt catctggaca gggcaactgg
     2221 gaggccatgc taagccgaga tacagactcc aggggactga atcaattaat ggtggatctt
     2281 attgtcaaat tgtcaagaac cccccccccc aggtcacaca tcaaatgaac agcagtgggc
     2341 agtgttcctc tctacctctg agcggaggga ggtatgggtc caaggaggga aaggtgatgg
     2401 tggggaactt ggtgatccat acagcccaca gtggcgaatg acacaagcaa gcaccctcat
     2461 ggatccccaa tgagaaaatt ccagacttgc agtagcctgg accagaaact cttgatgagc
     2521 tgggacagag ttcaagtgag tcacctaggt ccccttccct aaccccagcc ctgtgggtcc
     2581 ctgggagact tttttttttt tttttttttt ttttgtaaat gttctgctct ttttccacga
     2641 gctgtaggga gcacttcctt catagccctc agctcccctc agtccatctt tccccatctt
     2701 cccacctccg acagagctcc atggcaaact gggaaggggc ttttatatat taaaaaagaa
     2761 gcttttattg gaaaaaa
//
