LOCUS       NM_001416938            3715 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus cholinergic receptor, muscarinic 4 (Chrm4), transcript
            variant 2, mRNA.
ACCESSION   NM_001416938
VERSION     NM_001416938.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Berezovskaia,A., Thomsen,M., Fink-Jensen,A. and Wortwein,G.
  TITLE     A sex-specific effect of M4 muscarinic cholinergic autoreceptor
            deletion on locomotor stimulation by cocaine and scopolamine
  JOURNAL   Front Mol Neurosci 17, 1451010 (2024)
   PUBMED   39737113
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3715)
  AUTHORS   Song,S., Lin,Z., Zhao,C., Wen,J., Chen,J., Xie,S., Qi,H., Wang,J.
            and Su,X.
  TITLE     Vagal-mAChR4 signaling promotes Friend virus complex (FV)-induced
            acute erythroleukemia
  JOURNAL   Virol Sin 38 (3), 429-439 (2023)
   PUBMED   37172825
  REMARK    GeneRIF: Vagal-mAChR4 signaling promotes Friend virus complex
            (FV)-induced acute erythroleukemia.
REFERENCE   4  (bases 1 to 3715)
  AUTHORS   Wang,N., Langfelder,P., Stricos,M., Ramanathan,L., Richman,J.B.,
            Vaca,R., Plascencia,M., Gu,X., Zhang,S., Tamai,T.K., Zhang,L.,
            Gao,F., Ouk,K., Lu,X., Ivanov,L.V., Vogt,T.F., Lu,Q.R.,
            Morton,A.J., Colwell,C.S., Aaronson,J.S., Rosinski,J., Horvath,S.
            and Yang,X.W.
  TITLE     Mapping brain gene coexpression in daytime transcriptomes unveils
            diurnal molecular networks and deciphers perturbation gene
            signatures
  JOURNAL   Neuron 110 (20), 3318-3338 (2022)
   PUBMED   36265442
REFERENCE   5  (bases 1 to 3715)
  AUTHORS   Shindo,Y., Nakamura,H.M., Nakai,J., Wakamori,M. and Nakamura,T.
  TITLE     A parasympathetic neurotransmitter induces myoepithelial cell
            differentiation during salivary gland development
  JOURNAL   Exp Cell Res 416 (1), 113137 (2022)
   PUBMED   35427599
REFERENCE   6  (bases 1 to 3715)
  AUTHORS   Forrester,L.M., Nagy,A., Sam,M., Watt,A., Stevenson,L.,
            Bernstein,A., Joyner,A.L. and Wurst,W.
  TITLE     An induction gene trap screen in embryonic stem cells:
            Identification of genes that respond to retinoic acid in vitro
  JOURNAL   Proc Natl Acad Sci U S A 93 (4), 1677-1682 (1996)
   PUBMED   8643689
REFERENCE   7  (bases 1 to 3715)
  AUTHORS   Lammerding-Koppel,M., Greiner-Schroder,A. and Drews,U.
  TITLE     Muscarinic receptors in the prenatal mouse embryo. Comparison of
            M35-immunohistochemistry with [3H]quinuclidinyl benzylate
            autoradiography
  JOURNAL   Histochem Cell Biol 103 (4), 301-310 (1995)
   PUBMED   7648406
REFERENCE   8  (bases 1 to 3715)
  AUTHORS   Andre,C., Dos Santos,G. and Koulakoff,A.
  TITLE     Cultured neurons from mouse brain reproduce the muscarinic receptor
            profile of their tissue of origin
  JOURNAL   Eur J Neurosci 6 (11), 1691-1701 (1994)
   PUBMED   7874308
REFERENCE   9  (bases 1 to 3715)
  AUTHORS   Van Koppen,C.J., Sell,A., Lenz,W. and Jakobs,K.H.
  TITLE     Deletion analysis of the m4 muscarinic acetylcholine receptor.
            Molecular determinants for activation of but not coupling to the Gi
            guanine-nucleotide-binding regulatory protein regulate receptor
            internalization
  JOURNAL   Eur J Biochem 222 (2), 525-531 (1994)
   PUBMED   8020490
REFERENCE   10 (bases 1 to 3715)
  AUTHORS   van Koppen,C.J., Lenz,W. and Nathanson,N.M.
  TITLE     Isolation, sequence and functional expression of the mouse m4
            muscarinic acetylcholine receptor gene
  JOURNAL   Biochim Biophys Acta 1173 (3), 342-344 (1993)
   PUBMED   7916637
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL731772.9.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17784645.312644.1,
                                           ERR3363660.1737938.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1096              AL731772.9         59797-60892
            1097-3715           AL731772.9         64828-67446
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 50.63 cM"
     gene            1..3715
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /note="cholinergic receptor, muscarinic 4"
                     /db_xref="GeneID:12672"
                     /db_xref="MGI:MGI:88399"
     exon            1..1096
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..3715
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /inference="alignment:Splign:2.1.0"
     CDS             1129..2568
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /note="muscarinic acetylcholine receptor 4"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M4"
                     /protein_id="NP_001403867.1"
                     /db_xref="GeneID:12672"
                     /db_xref="MGI:MGI:88399"
                     /translation="MANFTPVNGSSANQSVRLVTTAHNHLETVEMVFIATVTGSLSLV
                     TVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTLYIIKGYWPLGAV
                     VCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSF
                     VLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHIS
                     LASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKPSIKKPPPGGASREELRNGKLEEAPP
                     PALPPPPRPVADKDTSNESSSGSATQNTKERPPTELSTTEAATTPALPAPTLQPRTLN
                     PASKWSKIQIVTKQTGSECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMA
                     ARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPERVWSIGYWLCYVNSTINP
                     ACYALCNATFKKTFRHLLLCQYRNIGTAR"
     regulatory      3687..3692
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /note="hexamer: ATTAAA"
     polyA_site      3715
                     /gene="Chrm4"
                     /gene_synonym="AChR M4; Chrm-4; M4; mm4 mAChR"
                     /note="major polyA site"
ORIGIN      
        1 gccacagcgc cgggagctgc aggcgaagca ggggccgccc cttggcaccc tgtccccgcc
       61 cccggccccc ggcccagtcg cccaccccac cgactcgcag ggcctctggc tagttccgcc
      121 gtctgtccgg cgccagcgga gcaagacaga agcagcgggt gagtaactcc gcggaatcgc
      181 gggggcgggg ggcttctatg caagatgagg atgtagggtt ctgggacccc ctgaaggtct
      241 ccagaggtgg gtcgggctgg aacagggccc cagacccctt tgaaaggcaa gcgcggtgcg
      301 gaaggggcgg agagctcaga gatggggcac ccctctggaa gaaggacccc aggtctctaa
      361 aaggtttctc taggtgaacc tctagggatt ggtgatcatt gagaaggcta ggggaggaat
      421 agtcccagcc cacagcccaa ccccgaagca gagcagccgt cgggtcagga cccacactcc
      481 ctcactccct cccgttggct cttcttcaag gcgccggaag atgaagcccc aaagcgcccg
      541 tttcccgccc gtctccctgc cctccgttcc caccttggcc caccgggagc ctccagtacg
      601 ctctagcccg ttggagtcct ctgtccgcga ccttgagccc cgacggaaaa cacccctgtt
      661 ctctcctgtt tcccttcccc ctctccagga gtaccctctc ttttactcat ccggggagat
      721 acatctccca gccctccacc ctcccagctc ttagaggaag tgttaggcac aatgatttgg
      781 cccaatatcc agcacccctc tctcttcccc taaagtacag aggcttctct ccgcctcagg
      841 aacagtcctg ggggccatag ctttcctgaa gtgatcactt aataatcgaa aatctacccc
      901 acgggcgccc agagcaccct ttcccgactt gtccccagca ggggtagggg ctcgagaacc
      961 cgcggaagca cattgcccac gcccagagac tacaggaact gcgtgcctgg gggttgatgg
     1021 gagggcgggg catcggagaa ccttggccct ttcgaccagg acccagacgt gatggagccg
     1081 ggagatgggc ccaaggccac tgcccacctg tctccagttg ggttcagcat ggcgaacttc
     1141 acacctgtca atggcagctc agccaatcag tctgtgcgcc tggtcacaac agcccacaac
     1201 cacctggaga cagtggagat ggtgttcatt gcgacagtga ctggttccct gagcctggtg
     1261 actgtggtgg gtaacatcct ggtgatgctg tccatcaagg tcaacaggca gttgcagaca
     1321 gtcaacaact acttcctgtt cagcctggcg tgtgcagatc tcatcatagg ggcgttctct
     1381 atgaaccttt acaccttata catcatcaag ggctactggc ccctgggtgc cgtggtctgt
     1441 gacctgtggc tggccctgga ctatgtggtg agcaatgcct ctgtcatgaa ccttctcatc
     1501 atcagctttg accgctattt ctgcgtcacc aagcccctca cctatccagc ccgccgcact
     1561 actaagatgg caggcctcat gattgcagcc gcctgggtct tgtcctttgt actctgggcc
     1621 cctgccatct tgttctggca gtttgtggtg ggcaagagga cagtgcctga taaccagtgc
     1681 ttcatccagt tcttgtccaa cccggcggtg accttcggca cagccattgc tgccttctac
     1741 ctgcctgtgg tcatcatgac ggtgctgtat attcatatct cgctggccag ccgcagccgt
     1801 gttcacaagc atcgacccga gggccccaag gagaagaagg ccaagactct ggctttcctc
     1861 aagagccctc tgatgaagcc gagcattaag aaacctccac cagggggcgc ttctcgagag
     1921 gaactgcgca acgggaagct agaagaggct cctccgccag ccctgccccc gcctccacgc
     1981 ccagtggctg acaaggacac ttccaatgag tccagctcag gcagtgccac ccagaacacc
     2041 aaggaacggc cacccacaga gctgtccacc acagaggccg ccaccacacc agcgctgccc
     2101 gctcctaccc tgcagccacg aaccctcaac ccagcctcca agtggtccaa gatccaaatt
     2161 gtgacaaagc agacaggcag tgaatgtgtg actgccatcg agatcgtacc tgccacgcca
     2221 gctggtatgc gcccagcagc caatgtggcc cgaaagtttg ccagcatcgc tcgtaaccag
     2281 gtgcgcaaga agcggcagat ggcggcccgg gagcgcaaag tgactcggac aatctttgcc
     2341 attctgctgg ccttcatcct cacctggaca ccctacaatg tcatggtcct ggtgaacacc
     2401 ttttgccaga gctgtatccc cgaaagggtg tggtccatcg gctactggct ctgctacgtc
     2461 aacagcacga tcaaccctgc ctgctatgca ctctgcaatg ccactttcaa aaagaccttc
     2521 cggcaccttt tgctgtgcca gtatcggaac atcggcacag ccaggtagac gggcaggtgc
     2581 cttaggaggt gctggtgtca agagtgtgta ccggggaacc acatggctca ccggctgtgg
     2641 aggagagagt aggcggcccc actctgagtt cgcatctgct gaagagaaca ggtctcccag
     2701 acaccgtgag gtacaagagg ctcttcgctg ggatgggaga gcagatctct actgaagcct
     2761 aggaggctcg gcccagggac ggtctgaact ggcaccacct ggcccatatc ttttaccttg
     2821 cctcaggaag atgggggacg atggctggag tgggcagtgg ctctatcaag gccaaccatc
     2881 ggtggaggtg gatgaataga gcagaacttg ggagcatctt gagaggagca aaaggagcca
     2941 ggcttgcaag ggtggggtcc cccgttatgc tgtaatcggt gctttccgcc ttcctgcgcc
     3001 ctcctctccc ttgcatgtag gctcagccct tcccctgccc ccaagtccca ctccaaggtg
     3061 agggacttcc ctgccactgt tgctcagcca ggctctggat gggaagccaa gccctttggc
     3121 actcccctgg ccagcagtca tctggacagg gcaactggga ggccatgcta agccgagata
     3181 cagactccag gggactgaat caattaatgg tggatcttat tgtcaaattg tcaagaaccc
     3241 ccccccccag gtcacacatc aaatgaacag cagtgggcag tgttcctctc tacctctgag
     3301 cggagggagg tatgggtcca aggagggaaa ggtgatggtg gggaacttgg tgatccatac
     3361 agcccacagt ggcgaatgac acaagcaagc accctcatgg atccccaatg agaaaattcc
     3421 agacttgcag tagcctggac cagaaactct tgatgagctg ggacagagtt caagtgagtc
     3481 acctaggtcc ccttccctaa ccccagccct gtgggtccct gggagacttt tttttttttt
     3541 tttttttttt ttgtaaatgt tctgctcttt ttccacgagc tgtagggagc acttccttca
     3601 tagccctcag ctcccctcag tccatctttc cccatcttcc cacctccgac agagctccat
     3661 ggcaaactgg gaaggggctt ttatatatta aaaaagaagc ttttattgga aaaaa
//
