LOCUS       NM_203491               5792 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus cholinergic receptor, muscarinic 2, cardiac (Chrm2),
            transcript variant 1, mRNA.
ACCESSION   NM_203491 XM_145245
VERSION     NM_203491.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Dudukcu,O., Raj,D.D.A., van de Haar,L.L., Grossouw,L.M.,
            Linders,L.E., Garritsen,O., Adolfs,Y., van Kronenburg,N.C.H.,
            Broekhoven,M.H., Kapteijns,T.H.W., Meye,F.J. and Pasterkamp,R.J.
  TITLE     Molecular diversity and migration of GABAergic neurons in the
            developing ventral midbrain
  JOURNAL   iScience 27 (11), 111239 (2024)
   PUBMED   39569362
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Fasciani,I., Petragnano,F., Wang,Z., Edwards,R., Telugu,N.,
            Pietrantoni,I., Zabel,U., Zauber,H., Grieben,M., Terzenidou,M.E.,
            Di Gregorio,J., Pellegrini,C., Santini,S. Jr., Taddei,A.R.,
            Pohl,B., Aringhieri,S., Carli,M., Aloisi,G., Marampon,F.,
            Charlesworth,E., Roman,A., Diecke,S., Flati,V., Giorgi,F.,
            Amicarelli,F., Tobin,A.B., Scarselli,M., Tokatlidis,K., Rossi,M.,
            Lohse,M.J., Annibale,P. and Maggio,R.
  TITLE     The C-terminus of the prototypical M2 muscarinic receptor localizes
            to the mitochondria and regulates cell respiration under stress
            conditions
  JOURNAL   PLoS Biol 22 (4), e3002582 (2024)
   PUBMED   38683874
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5792)
  AUTHORS   Shindo,Y., Nakamura,H.M., Nakai,J., Wakamori,M. and Nakamura,T.
  TITLE     A parasympathetic neurotransmitter induces myoepithelial cell
            differentiation during salivary gland development
  JOURNAL   Exp Cell Res 416 (1), 113137 (2022)
   PUBMED   35427599
REFERENCE   4  (bases 1 to 5792)
  AUTHORS   Szabo,G.G., Farrell,J.S., Dudok,B., Hou,W.H., Ortiz,A.L., Varga,C.,
            Moolchand,P., Gulsever,C.I., Gschwind,T., Dimidschstein,J.,
            Capogna,M. and Soltesz,I.
  TITLE     Ripple-selective GABAergic projection cells in the hippocampus
  JOURNAL   Neuron 110 (12), 1959-1977 (2022)
   PUBMED   35489331
REFERENCE   5  (bases 1 to 5792)
  AUTHORS   Meier,A.M., Wang,Q., Ji,W., Ganachaud,J. and Burkhalter,A.
  TITLE     Modular Network between Postrhinal Visual Cortex, Amygdala, and
            Entorhinal Cortex
  JOURNAL   J Neurosci 41 (22), 4809-4825 (2021)
   PUBMED   33849948
REFERENCE   6  (bases 1 to 5792)
  AUTHORS   Shapiro,M.S., Loose,M.D., Hamilton,S.E., Nathanson,N.M., Gomeza,J.,
            Wess,J. and Hille,B.
  TITLE     Assignment of muscarinic receptor subtypes mediating G-protein
            modulation of Ca(2+) channels by using knockout mice
  JOURNAL   Proc Natl Acad Sci U S A 96 (19), 10899-10904 (1999)
   PUBMED   10485923
REFERENCE   7  (bases 1 to 5792)
  AUTHORS   Gomeza,J., Shannon,H., Kostenis,E., Felder,C., Zhang,L.,
            Brodkin,J., Grinberg,A., Sheng,H. and Wess,J.
  TITLE     Pronounced pharmacologic deficits in M2 muscarinic acetylcholine
            receptor knockout mice
  JOURNAL   Proc Natl Acad Sci U S A 96 (4), 1692-1697 (1999)
   PUBMED   9990086
REFERENCE   8  (bases 1 to 5792)
  AUTHORS   Matsui,M., Araki,Y., Karasawa,H., Matsubara,N., Taketo,M.M. and
            Seldin,M.F.
  TITLE     Mapping of five subtype genes for muscarinic acetylcholine receptor
            to mouse chromosomes
  JOURNAL   Genes Genet Syst 74 (1), 15-21 (1999)
   PUBMED   10549128
REFERENCE   9  (bases 1 to 5792)
  AUTHORS   Lammerding-Koppel,M., Greiner-Schroder,A. and Drews,U.
  TITLE     Muscarinic receptors in the prenatal mouse embryo. Comparison of
            M35-immunohistochemistry with [3H]quinuclidinyl benzylate
            autoradiography
  JOURNAL   Histochem Cell Biol 103 (4), 301-310 (1995)
   PUBMED   7648406
REFERENCE   10 (bases 1 to 5792)
  AUTHORS   Shapiro,R.A., Scherer,N.M., Habecker,B.A., Subers,E.M. and
            Nathanson,N.M.
  TITLE     Isolation, sequence, and functional expression of the mouse M1
            muscarinic acetylcholine receptor gene
  JOURNAL   J Biol Chem 263 (34), 18397-18403 (1988)
   PUBMED   2848036
  REMARK    Erratum:[J Biol Chem 1989 Apr 15;264(11):6596]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113246.15 and AC159505.7.
            
            On Aug 31, 2022 this sequence version replaced NM_203491.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.6471.1,
                                           SRR17784648.493793.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-175               AC113246.15        60474-60648         c
            176-323             AC113246.15        60089-60236         c
            324-5792            AC159505.7         87735-93203         c
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 15.48 cM"
     gene            1..5792
                     /gene="Chrm2"
                     /gene_synonym="AChR-M2; Chrm-2; M2"
                     /note="cholinergic receptor, muscarinic 2, cardiac"
                     /db_xref="GeneID:243764"
                     /db_xref="MGI:MGI:88397"
     exon            1..175
                     /gene="Chrm2"
                     /gene_synonym="AChR-M2; Chrm-2; M2"
                     /inference="alignment:Splign:2.1.0"
     exon            176..323
                     /gene="Chrm2"
                     /gene_synonym="AChR-M2; Chrm-2; M2"
                     /inference="alignment:Splign:2.1.0"
     exon            324..5792
                     /gene="Chrm2"
                     /gene_synonym="AChR-M2; Chrm-2; M2"
                     /inference="alignment:Splign:2.1.0"
     CDS             364..1764
                     /gene="Chrm2"
                     /gene_synonym="AChR-M2; Chrm-2; M2"
                     /note="muscarinic acetylcholine receptor 2; AChR M2"
                     /codon_start=1
                     /product="muscarinic acetylcholine receptor M2"
                     /protein_id="NP_987076.2"
                     /db_xref="CCDS:CCDS20004.1"
                     /db_xref="GeneID:243764"
                     /db_xref="MGI:MGI:88397"
                     /translation="MNNSTNSSNNGLAITSPYKTFEVVFIVLVAGSLSLVTIIGNILV
                     MVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLAL
                     DYVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAIL
                     FWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIK
                     KEKKEPVANQDPVSPSLVQGRIVKPNNNNMPGGDGGLEHNKIQNGKAPRDGGTENCVQ
                     GEEKESSNDSTSVSAVASNMRDDEITQDENTVSTSLGHSKDDNSRQTCIKIVTKTQKG
                     DACTPTSTTVELVGSSGQNGDEKQNIVARKIVKMTKQPAKKKPPPSREKKVTRTILAI
                     LLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYINSTINPACYALCNATFKKT
                     FKHLLMCHYKNIGATR"
     regulatory      5773..5778
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chrm2"
                     /gene_synonym="AChR-M2; Chrm-2; M2"
                     /note="hexamer: AATAAA"
     polyA_site      5792
                     /gene="Chrm2"
                     /gene_synonym="AChR-M2; Chrm-2; M2"
                     /note="major polyA site"
ORIGIN      
        1 gcagatccca cttggaaatc gccagaggca ctgggagaag tggaggagcg cagctcccag
       61 acactaatta ctacttcccc ggcactaggg tgaagaaatg aagcccgccc agctcttctc
      121 cacacccagg tctcctttct gtttcggtgt taactgtcat cttcctgaaa gccagatcca
      181 gggagaaggc aagcgggcaa ccagctgagg actccgagct gtgccgctcg ctcccaaacc
      241 ggtccaacct gagcccgcca aaggctcctc gctccagcca gactccacca gatcgcagcc
      301 ccgggcgact aagtcagtga caggtttaaa tgtttctttg gccacttgac tactgaacac
      361 aaaatgaata actcaactaa ctcctcgaac aatggtttgg ctattaccag tccttacaag
      421 acatttgaag tggtatttat tgtccttgtg gctggatccc tcagtctggt gaccatcatt
      481 gggaacattc tagtcatggt ttccattaaa gtcaaccgcc accttcagac tgtcaacaat
      541 tacttcttgt tcagcctggc ctgtgctgac ctcatcatag gtgttttctc catgaacttg
      601 tataccctct acactgtgat tggctactgg cctttgggac ctgtagtgtg cgacctttgg
      661 ctagccttgg actatgttgt cagcaatgcc tccgttatga atcttctcat catcagcttt
      721 gatagatact tctgtgtcac aaaacctcta acctacccag ttaagcggac cacaaaaatg
      781 gcaggcatga tgattgcagc tgcgtgggtt ctttccttca tcctctgggc cccagccatt
      841 ctcttctggc agttcatcgt aggggtaagg actgtggaag acggggagtg ctacattcag
      901 ttcttttcca acgctgccgt cacctttggc actgccattg cggctttcta tctgcctgtc
      961 atcatcatga ctgtgctcta ttggcacata tcccgggcga gcaagagcag aataaagaaa
     1021 gaaaagaagg aaccagtggc caaccaagac ccggtgtctc cgagtctagt gcaaggaaga
     1081 attgtaaagc caaacaacaa caacatgcct ggtggtgatg gtggcctgga gcacaacaag
     1141 atccagaatg gcaaggctcc gcgggacggt gggactgaaa actgcgttca gggggaggag
     1201 aaagaaagct ccaacgactc cacgtctgtc agtgccgtgg cctccaacat gagagatgat
     1261 gagataaccc aggatgaaaa cacggtttcc acttccctgg gccactccaa agatgacaac
     1321 tctaggcaga catgcatcaa aattgtcacc aagacccaaa agggtgacgc atgcacacca
     1381 acaagtacca cagtagaact agtgggatcg tcaggtcaga atggtgatga aaagcagaac
     1441 attgtagccc gcaaaattgt gaagatgacc aagcagcctg ccaaaaagaa gcctcctcca
     1501 tcccgggaaa agaaagtgac caggacaatc ttggctatcc tgttggcttt catcatcacg
     1561 tgggcgccat acaatgtcat ggtgctcatc aataccttct gtgcaccctg catccccaat
     1621 acagtgtgga caattggcta ctggctctgt tacattaata gcaccatcaa ccctgcctgc
     1681 tatgcacttt gtaacgccac cttcaaaaag acttttaagc acctccttat gtgtcattac
     1741 aagaacatag gcgctacaag gtaaaagacc ttttcaaaag aagaaatgtg gtcgagtgtg
     1801 tcttggggaa gaacagggac aagaaagctg tgtttatagt gatctgccct tgcactttac
     1861 aatcttacta caacgtgcaa ttaaggggtt ttagagagac actcttattg tacctatgct
     1921 ccagtttgaa aaataaataa ataaataacc ttcaccgtat aaaccctgtc cgtttaggcg
     1981 caccgagaca atgaaagaga cacgctgaaa ttgcagatct aaggaacaat ctctactgcc
     2041 tcctgctctc ttgggagaag ggcttcagaa tctacaattt catgtctctg cacaagaaga
     2101 gtaaccttgt tagtttgttg tttctttctg ttattccccc gcgtggtgat gagaaagaaa
     2161 tggcgcatta catgctaaca cggagaccta aacacaaaga agcaggcact atacaatgag
     2221 agataagaag aaagaaaatc taataggatg cagagatggt atggagagcc ccgaacatat
     2281 tcccggcaat gctgtgcgct gatctgaagg gttttaatgg aatcactgtt catttctcct
     2341 ctcttccttt cccatcatga aaagcaagaa aacaaaacag caaaagccca actaggccat
     2401 actatttttt tctactattt ttgtagttcc tggttttata ttgtatatgg atagtatctg
     2461 caggacttct aaatctggcc agagtcaccc atgaaaatgg acactctgcc actccccact
     2521 gtgtcgatgt actctttgat ggtgtacaac ctgtaaggat ttcactcact tgagttcctt
     2581 tgtgtggaaa atttttaccc atgtgacttc tcaactaatc cattttaaca atgtagtctg
     2641 cctaagacat aaatatgctt gggaaaccaa agtcatttaa acttaatttt taatcacatt
     2701 agaaaaatgg tcattctagc tggagaaagt tcactgaggc aagatgaagt ttgagatgag
     2761 aagacagaag gtcagaaggg cgatctgttt gaacttgaac ctgcctgacc tgtgtgcaca
     2821 atgttaatgt gtacaacatg aactcctgac tttgtcagca ggaatatgat gttggttttc
     2881 tcatttggag tctcaattag tttactgtgt caaccccaag aggaaaagac cttatattca
     2941 atgtctgtta gcagatggta ctcaaattat attcaaatga agttttctat aatcatacta
     3001 agacatgggg tggggaaatg ggtcatgcaa aaatatttac taatactacc tgtttcatgt
     3061 tcttccagtt gtcgtcaaat ctctgtaaga ttatattgct tcaaatttca ctaatcagga
     3121 gagttatatt aatctttgtg gcttgaatat aatgaaaaaa ccaaacagag tcaagatatg
     3181 cctgcataat ctgatggaag aatacatgtg attctggaca tgccatttcc cacagtctaa
     3241 tataaccaca actagctgtc acaaaaaatg attattaaag ggtaacacta aacatataat
     3301 gtgagtatag aaaatccaca cttgacactc taaaataaat gcagaattac accaatctac
     3361 atgaactagt tagatttttg tcttcatata atttcttcca ttatgttatg gtatttcaat
     3421 cagattttct aagtgtgggc ctagtagata ggaattaaag ttataaattc tgttataaag
     3481 ttcagatttt cactgtgtgc tctgattcct cctccgccat tccatgagta ttgcaaggca
     3541 ggttcaacag cgtgtagtcc tcagagctac cagagttctt ttgtctgact gcatgtcagg
     3601 aacatctcag caagtgggtg ataaaggata ggacagcagc aacagcaaca ttgtaagcta
     3661 gcaagaagca agttgctaac aatatatgta tttattcttt aggaacagaa cttactgttt
     3721 gagtaatttc agttagtttt aactccaaaa tctcctatat gtaaacatga gttcggttca
     3781 ggttaattta gtctattaat tagttgatgt agttccctta gttcagaatt actaaaatgt
     3841 aaattttgaa atttaagatc acatttattt ttgtgttgtt tattcaacta attgagtgga
     3901 gaaaacttca ctccaattga tttcttgctt tcctgtaaga aatgttatca atctgtatta
     3961 tatgctatgt gatacagaca tggccaccat tgggaggcca ggattcattc tctgtttgct
     4021 ttaagcccaa tccaccatga gctaatcttt gtcttgttct actgtaatct ttccttgatt
     4081 ctttctgtgc ctagcaattt gccacagcta ccacatctta aaagagaaac tgtcaagttc
     4141 aagaagaaag tgaaggttgt ctaacatacc atggaaaatt tccaaaagaa tcagaaaaaa
     4201 aaggggggga cattggcaag tttggaggga gaatgtattc aatgatcatt ctttactgtt
     4261 gctagttttg ctttgttttt aatgactcac acgcagaggc aatttctacc ctatggatac
     4321 ttagtgataa gtagggccat tttgatggtc acaacatgga ataggcattt gtgtctaata
     4381 agaaggagtt ttgaatgcac aagctatgct cccccctccc aatgatttgg ctcaaaatgg
     4441 cagtgatacc aggactgaga aagactagtt tatattaagg gatctctgaa gattccttta
     4501 ataaccctcc atcatttaaa caaacaaaca gaaaagatta atgcagcttg ctttcctgtg
     4561 agcaaagctc actaactcag aggctatcat caattctcct gcattaattt agatttgaaa
     4621 tttacagata agagcacaga ccacacctat ggaaaccagc agcttccaca cacctagagc
     4681 actgcagcat aaccaatgcc tggctatgga atcacagccc ctacttgaga ggctatggct
     4741 ttgtccgtga acatcactgc ttctagtctg atcagtttct actggaagca tcatttcttt
     4801 tcttttcaaa ttaatgaaga gccctttaaa gaaacagtat gactcatctt ctctgctttc
     4861 actagaactg tcagaagctg tcaagccttt atggtttttt tttctcctca tagcaataat
     4921 gtaaatatcc cagagtggga ttacaaaaag aagattaaga atataaaaaa gaggagccag
     4981 ctcacttcta gagtaaataa atgattgcct catgaccaag tcatgattat gttaatggca
     5041 tatcccaagt attgggacaa ggtcgccaat atagtaagct acaaaggata agataaacca
     5101 tgtggcttga tatatggtat cctgaatcgt acttccttct gctttttgct tacttgatgc
     5161 tgtttgatcc agaagaaatg atggcatgag agggaattag atttcaaaat aataccaata
     5221 gttatttacc aatgcagcca acttttattt tcttgtattt agttcatcca ctgttgctcc
     5281 cctttccaat ttgatatctt caccaacaac cataacactg gtgatacaaa tagagtagga
     5341 aggcaccccc agctggatga gacatgtatc ctcagcatgt atatacaacg ccatgccttt
     5401 ccattaggct tctcttaaaa gggtgcacgc tgtagcatga actctgtgta tatttaatat
     5461 accttccaat gatatgctgt atgcttcata tatatattat ataaataaaa taaaatgtat
     5521 aatagaaata aatatttctg tatgcaaata aatatattat acacaaaatt gtaacagaca
     5581 ctctggcata tatgttgctt cttaggaata agagtagatt taatgggcaa aaccatttta
     5641 aaagaagatc atattttgca tcgaaggaag agatttaaga acagccaaac tcctattcac
     5701 attaatattc aactgattta tcatctttga gtgtctgaca ctgattttag agatcttttg
     5761 gaggaataaa ttaataaatc ttgctggtat ca
//
