LOCUS       NM_133709               1520 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus chordin-like 2 (Chrdl2), transcript variant 2, mRNA.
ACCESSION   NM_133709
VERSION     NM_133709.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souilhol,C., Gonneau,C., Lendinez,J.G., Batsivari,A., Rybtsov,S.,
            Wilson,H., Morgado-Palacin,L., Hills,D., Taoudi,S., Antonchuk,J.,
            Zhao,S. and Medvinsky,A.
  TITLE     Inductive interactions mediated by interplay of asymmetric
            signalling underlie development of adult haematopoietic stem cells
  JOURNAL   Nat Commun 7, 10784 (2016)
   PUBMED   26952187
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Forsman,C.L., Ng,B.C., Heinze,R.K., Kuo,C., Sergi,C.,
            Gopalakrishnan,R., Yee,D., Graf,D., Schwertfeger,K.L. and Petryk,A.
  TITLE     BMP-binding protein twisted gastrulation is required in mammary
            gland epithelium for normal ductal elongation and myoepithelial
            compartmentalization
  JOURNAL   Dev Biol 373 (1), 95-106 (2013)
   PUBMED   23103586
REFERENCE   3  (bases 1 to 1520)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   4  (bases 1 to 1520)
  AUTHORS   Zakin,L., Metzinger,C.A., Chang,E.Y., Coffinier,C. and De
            Robertis,E.M.
  TITLE     Development of the vertebral morphogenetic field in the mouse:
            interactions between Crossveinless-2 and Twisted Gastrulation
  JOURNAL   Dev Biol 323 (1), 6-18 (2008)
   PUBMED   18789316
REFERENCE   5  (bases 1 to 1520)
  AUTHORS   Oren,A., Toporik,A., Biton,S., Almogy,N., Eshel,D., Bernstein,J.,
            Savitsky,K. and Rotman,G.
  TITLE     hCHL2, a novel chordin-related gene, displays differential
            expression and complex alternative splicing in human tissues and
            during myoblast and osteoblast maturation
  JOURNAL   Gene 331, 17-31 (2004)
   PUBMED   15094188
REFERENCE   6  (bases 1 to 1520)
  AUTHORS   Nakayama,N., Han,C.Y., Cam,L., Lee,J.I., Pretorius,J., Fisher,S.,
            Rosenfeld,R., Scully,S., Nishinakamura,R., Duryea,D., Van,G.,
            Bolon,B., Yokota,T. and Zhang,K.
  TITLE     A novel chordin-like BMP inhibitor, CHL2, expressed preferentially
            in chondrocytes of developing cartilage and osteoarthritic joint
            cartilage
  JOURNAL   Development 131 (1), 229-240 (2004)
   PUBMED   14660436
  REMARK    GeneRIF: CHL2 expression is restricted to chondrocytes of various
            developing joint cartilage surfaces and connective tissues in
            reproductive organs.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113298.14, AF338222.1 and AC161205.3.
            
            On Apr 30, 2009 this sequence version replaced NM_133709.2.
            
            Transcript Variant: This variant (2) contains an alternate first
            exon in place of that of variant 1. The resulting isoform (2) has a
            shorter and distinct N-terminus compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019399.1, AF338222.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                AC113298.14        15951-16046         c
            97-1323             AF338222.1         1-1227
            1324-1363           AC161205.3         114765-114804
            1364-1520           AC161205.3         115638-115794
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 54.29 cM"
     gene            1..1520
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /note="chordin-like 2"
                     /db_xref="GeneID:69121"
                     /db_xref="MGI:MGI:1916371"
     exon            1..244
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..1443
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; chordin-like protein 2"
                     /codon_start=1
                     /product="chordin-like protein 2 isoform 2 precursor"
                     /protein_id="NP_598470.3"
                     /db_xref="CCDS:CCDS21492.1"
                     /db_xref="GeneID:69121"
                     /db_xref="MGI:MGI:1916371"
                     /translation="MVPGVRIIPSLLGLVMFWLPLDSQALSRSGKVCLFGEKIYTPGQ
                     SWHPYLEPQGTIYCVRCTCSENGHVNCYRLRCPPLHCSQPVMEPQQCCPRCVDPHVPS
                     GLRVPLKSCQLNETTYQHGEIFSAQELFPARLSNQCVLCSCIEGHTYCGLMTCPEPSC
                     PTTLPLPDSCCQTCKDRTTESSTEENLTQLQHGERHSQDPCSERRGPSTPAPTSLSSP
                     LGFIPRHFQSVGMGSTTIKIILKEKHKKACTHNGKTYSHGEVWHPTVLSFGPMPCILC
                     TCIDGYQDCHRVTCPTQYPCSQPKKVAGKCCKICPEDEAEDDHSEVISTRCPKVPGQF
                     QVYTLASPSPDSLHRFVLEHEASDQVEMYIWKLVKGIYHLVQIKRVRKQDFQKEAQNF
                     RLLTGTHEGYWTVFLAQTPELKVTASPDKVTKTL"
     exon            245..357
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            358..451
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            452..594
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            595..688
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            689..744
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            745..901
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            902..1096
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1097..1270
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1363
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1520
                     /gene="Chrdl2"
                     /gene_synonym="1810022C01Rik; Chl2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcatcctcac cttagacctc tcctgtcctt ggctcctctt catctttgct tttccgactc
       61 ctcaagcagc ggtcctactt ggtcctctga ggacttactt gtgtccttat ctcactttct
      121 cccggctcat cccggggttg tctgaccttg ggacaaggaa ggatggttcc cggggtgagg
      181 atcatcccct ctttgctggg actcgtgatg ttctggctcc cgttggactc gcaagcacta
      241 tcccgctcgg gcaaagtctg ccttttcggt gaaaagatat atacccccgg ccagagctgg
      301 cacccctact tggaaccaca aggcacgata tactgcgtgc gctgtacctg ctctgagaat
      361 ggacatgtga attgttaccg cctccgctgc ccaccccttc actgctcaca gcctgtgatg
      421 gagccacagc aatgctgtcc caggtgtgtg gatcctcatg tcccctctgg cctccgagtt
      481 cccctaaagt cctgccagct caatgagacc acataccaac atggagagat cttcagtgcc
      541 caggagctgt tccctgcccg cctgtccaac cagtgtgtcc tgtgtagctg tattgaaggc
      601 cacacttact gtggtctcat gacctgtcct gaacccagct gccccaccac actccctctg
      661 cctgattcct gctgtcagac ctgcaaagac aggacaactg agagttccac agaagaaaac
      721 ttgacacagc tgcagcatgg agagagacat tcccaggatc catgctcgga gaggagaggc
      781 cccagcacgc cagcccccac cagcctcagc tcccctctgg gcttcatccc tcgccacttc
      841 cagtcagtag gaatgggcag cacaaccatc aagattatct tgaaggagaa acataaaaaa
      901 gcttgcacac acaatgggaa gacatactcc catggggagg tgtggcaccc cactgtgctc
      961 tcctttggcc ccatgccctg catcctgtgc acatgtatcg atggctacca ggactgccac
     1021 cgtgtgacct gccccaccca atatccctgc agtcaaccca agaaagtggc tgggaagtgc
     1081 tgcaagatct gcccagagga cgaggcggaa gatgaccaca gtgaggtcat ttccacccgg
     1141 tgtcccaagg taccaggcca gttccaggtg tacacgttgg catctccaag cccagacagc
     1201 ctacaccgct ttgtcctgga gcatgaagcc tctgaccagg tagagatgta catttggaag
     1261 ctggtgaaag gaatctacca cttggttcag atcaagagag tcaggaagca agatttccag
     1321 aaagaggctc agaacttccg gctgctcacc ggcacccatg aaggttactg gaccgtcttc
     1381 ctagcccaga ctccagagct gaaagttaca gccagcccag acaaagtgac caagacatta
     1441 tagcaaggac ctaaagagtt gcagatacga gttttattgg ttttgttatt atatattaat
     1501 aaagaagtcg cattaccctc
//
