LOCUS       NM_133913               3730 bp    mRNA    linear   ROD 28-NOV-2023
DEFINITION  Mus musculus chondroitin polymerizing factor 2 (Chpf2), transcript
            variant 1, mRNA.
ACCESSION   NM_133913
VERSION     NM_133913.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Shimbo M, Suzuki R, Fuseya S, Sato T, Kiyohara K, Hagiwara K, Okada
            R, Wakui H, Tsunakawa Y, Watanabe H, Kimata K, Narimatsu H, Kudo T
            and Takahashi S.
  TITLE     Postnatal lethality and chondrodysplasia in mice lacking both
            chondroitin sulfate N-acetylgalactosaminyltransferase-1 and -2
  JOURNAL   PLoS One 12 (12), e0190333 (2017)
   PUBMED   29287114
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Kozomara A and Griffiths-Jones S.
  TITLE     miRBase: integrating microRNA annotation and deep-sequencing data
  JOURNAL   Nucleic Acids Res 39 (Database issue), D152-D157 (2011)
   PUBMED   21037258
REFERENCE   3  (bases 1 to 3730)
  AUTHORS   Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M,
            Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice
            DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson
            A, Zambrowicz BP and de Sauvage FJ.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   4  (bases 1 to 3730)
  AUTHORS   Izumikawa T, Koike T, Shiozawa S, Sugahara K, Tamura J and Kitagawa
            H.
  TITLE     Identification of chondroitin sulfate glucuronyltransferase as
            chondroitin synthase-3 involved in chondroitin polymerization:
            chondroitin polymerization is achieved by multiple enzyme complexes
            consisting of chondroitin synthase family members
  JOURNAL   J Biol Chem 283 (17), 11396-11406 (2008)
   PUBMED   18316376
REFERENCE   5  (bases 1 to 3730)
  AUTHORS   Sakai K, Kimata K, Sato T, Gotoh M, Narimatsu H, Shinomiya K and
            Watanabe H.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a
            critical role in chondroitin sulfate synthesis in cartilage
  JOURNAL   J Biol Chem 282 (6), 4152-4161 (2007)
   PUBMED   17145758
REFERENCE   6  (bases 1 to 3730)
  AUTHORS   Okazaki N, Kikuno R, Ohara R, Inamoto S, Aizawa H, Yuasa S,
            Nakajima D, Nagase T, Ohara O and Koga H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   7  (bases 1 to 3730)
  AUTHORS   Silbert,J.E. and Freilich,L.S.
  TITLE     Biosynthesis of chondroitin sulphate by a Golgi-apparatus-enriched
            preparation from cultures of mouse mastocytoma cells
  JOURNAL   Biochem J 190 (2), 307-313 (1980)
   PUBMED   6781479
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK122504.1 and BC036970.1.
            
            On Oct 26, 2007 this sequence version replaced NM_133913.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.206649.1,
                                           SRR1660811.188527.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2116              AK122504.1         1-2116
            2117-3715           AK122504.1         2764-4362
            3716-3730           BC036970.1         1090-1104
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5"
     gene            1..3730
                     /gene="Chpf2"
                     /gene_synonym="2010209O12Rik; Gm42230; mir-671; mKIAA1402"
                     /note="chondroitin polymerizing factor 2"
                     /db_xref="GeneID:100910"
                     /db_xref="MGI:MGI:1917522"
     exon            1..1551
                     /gene="Chpf2"
                     /gene_synonym="2010209O12Rik; Gm42230; mir-671; mKIAA1402"
                     /inference="alignment:Splign:2.1.0"
     CDS             1289..3595
                     /gene="Chpf2"
                     /gene_synonym="2010209O12Rik; Gm42230; mir-671; mKIAA1402"
                     /EC_number="2.4.1.226"
                     /codon_start=1
                     /product="chondroitin sulfate glucuronyltransferase
                     precursor"
                     /protein_id="NP_598674.2"
                     /db_xref="CCDS:CCDS51436.1"
                     /db_xref="GeneID:100910"
                     /db_xref="MGI:MGI:1917522"
                     /translation="MRLSSLLALLRPVLPLILGLSLGCSLSLLRVSWIQGEGEDPCVE
                     AVGKPGGPQNPDSKNGLDQGDEDFRPRIVPYYRDPNKPYKKVLRTRYIQTELGSRERL
                     LVAVLTSRATLSTLAVAVNRTVAHHFPRLLYFTGQRGARAPAGMQVVSHGDERPAWLM
                     SETLRHLHTHFGADYDWFFIMQDDTYVQAPRLAALAGHLSINQDLYLGHAEEFIGTGE
                     QARYCHGGFGYLLSRSLLLRLRPHLDGCRGDILSARPDEWLGRCLIDTLGIGCVSQHQ
                     GQQYRSFELAKNRDPEKEESPAFLSAFAVHPVPEGTLMYRLHKRFSALELERAYSEIE
                     QLQAQIRNLTVLTPEGEAGLSWPVGLPAPFTPHSRFEVLGWDYFTEQHTFACADGAPK
                     CPLQGASRADVGDAVDTALEQLNRRYQPRLRFRKQRLLNGYRRFDPARGMEYTLDLLL
                     EAVTQRGHRRSLARRVSLLRPLSRVEILPMPYVTEATRVQLVLPLLVAEAPAALAFLE
                     AFAASVLEPREHALLTLLLVYGPREGRGGPDPFLGVKAAAAELERRYPGARLAWLAVR
                     AEAPSQVRLMDVISKKHPVDTLFFLTTVWTRPGSEVLNRCRMNAISGWQAFFPVHFQE
                     FNPALSPQRSPPGVPGADPPSPGVDPSRGAPVGGRFDRQASAEGCFYNADYLAARARL
                     AGELAGQEEEEALEGLEVMDVFLRFSGLHLFRAVEPGLVQKFSLRDCSPRLSEELYHR
                     CRLSNLEGLGARTQLAMALFEQEQANST"
     exon            1552..2116
                     /gene="Chpf2"
                     /gene_synonym="2010209O12Rik; Gm42230; mir-671; mKIAA1402"
                     /inference="alignment:Splign:2.1.0"
     exon            2117..2299
                     /gene="Chpf2"
                     /gene_synonym="2010209O12Rik; Gm42230; mir-671; mKIAA1402"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..3718
                     /gene="Chpf2"
                     /gene_synonym="2010209O12Rik; Gm42230; mir-671; mKIAA1402"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagggctggc ttgtgaggtg gcagcggcag caaagcggag ctcctcggcc cgccacaggg
       61 tgggctcacc cgcgaggcgg ctccgtctgc cgtggggctt gggtgttcct gttcccctgc
      121 ccggcaggac ctgagaaccc gctcggtcct gctgtccgcc gcgagctccc ctccaccgcc
      181 caagttcgca gagcaaacca gctggacgca aggccttttc tgcagtcgtg ggacgtaggc
      241 gattaatttc tgtctccaga cggccggcta ggactcgctc tgtcccttct tctgggggct
      301 ggcacggtgc tgtggtcgcc aagggaataa tagggcactg actggcgccc tattggaagg
      361 tgccctgttg gaaggtggtg agcctctgct ggccttccca gacccccgcg tttctctaac
      421 tgctgctgtc tcccggcttg cttttgcttt atttggcttt gtttgataac ctctagccac
      481 agcaagagtg tctgtaattt gtctcctgct gttcttagat atgtgggcag catagctttg
      541 caggacgagt aggatggcta gcaaaatggc tccatcagcc attgcgctcc tatgagaggc
      601 cagctgtgct gagtcactgt gactgctcac ctcatttggg tgggtaggtg ggtgggcacc
      661 attcatagct ggtgatctta tctgacaagg aggggatttc tagagtctgt ggctctcttt
      721 gacactcagg agcggggtgg aaggaggtgc tgaccccttc tctagtgctt gcctgcagca
      781 gcggcagatc agcagttcct ctggggcctg aggagacata gtgggccagg atagcttcag
      841 catggcacag ggtgtctgag gcaggccctt ttggtcagac tgttgttgga atggcgcatg
      901 cactttgccc taagctctgt gtcactgggt gctgctgact tgaattaggc ccatgtttca
      961 aatggctgta gaccttctgg ggttaggttt ggcaggactg cactttcttt tttctttttc
     1021 ctggactaca gactgcaatc cccgtaggga gtggcattgc tcacatagac attagtattt
     1081 tactgagggt atcagcacca tggccactga agaccagaca tagggttggg aagacttggt
     1141 ggggatgctg ggctcctttt tctgttgctg acctcgttga ctccgggttc tctgggtaaa
     1201 accgtgggct actgctggcc tgtggcccat caacctactc atccttgaga ctgtgcccct
     1261 ggggcgccct gtgtcagggc ctgccaccat gcgactgagc tccctgctgg ctctgctgcg
     1321 gccagtgctc cccctcatcc taggactgtc tctgggatgc agcctgagcc tcctgcgggt
     1381 ttcctggatc cagggtgagg gagaagatcc ctgtgtagag gctgtgggga agccaggagg
     1441 gccacagaat ccagactcaa aaaatgggct ggaccaaggc gatgaagact tcagacccag
     1501 gattgtgcct tactacaggg atcctaacaa gccctacaag aaggtgctca ggactcggta
     1561 tatacagaca gagctgggct ctcgtgagcg cctgctggtg gctgtcctga cttctcgagc
     1621 cacactgtcc accctggccg tcgcggttaa ccgtacagta gcacatcact tccctcggtt
     1681 actctacttc acagggcagc gaggggcccg ggctcctgca gggatgcagg tggtgtctca
     1741 tggagatgag cggcctgcct ggctcatgtc agagaccctg cgccaccttc acacacactt
     1801 tggggcagac tatgactggt tcttcatcat gcaggacgac acatatgtgc aggcccccag
     1861 attggcagct cttgctggtc accttagcat caaccaagac ctgtacttag gccatgcaga
     1921 ggaattcatt ggtacaggag agcaggcccg gtactgccat gggggctttg gctacctgtt
     1981 gtcacggagt ctcctattgc gcttgcggcc acatctggat ggctgccgag gggacattct
     2041 cagtgcccgg cctgatgaat ggcttggccg ctgcctcatc gacactctgg gcatcggctg
     2101 tgtctcacag caccaggggc aacagtatcg ctccttcgaa ctggccaaga atagggaccc
     2161 tgagaaggag gagagccctg ctttcctgag tgccttcgcc gttcacccgg tccccgaggg
     2221 caccctcatg taccggctcc acaaacgctt cagtgctctg gagttggagc gggcttacag
     2281 cgaaatagaa caactgcagg cccagatccg gaacctgacg gtgctgaccc ctgaggggga
     2341 ggcagggcta agctggcctg ttgggctccc ggcccctttc actccacact ctcgcttcga
     2401 ggtgctgggc tgggactact tcacggagca acacaccttc gcctgtgcag acggggctcc
     2461 caagtgtccc ctgcaggggg ctagcagggc agatgtaggg gacgctgtgg acacagctct
     2521 ggagcagctc aatcggcgct atcagccccg cctgcgcttc cggaagcagc ggcttctcaa
     2581 tggctaccgg cgctttgacc ctgcaagggg catggaatac accctcgact tgctgctgga
     2641 agctgtgact cagcgagggc acagacgctc cctggcccgc agagtgagcc tgctgaggcc
     2701 actgagcaga gtggaaatcc tgcctatgcc ctatgtcacc gaggctaccc gggtgcaact
     2761 ggtgctgcca ctcctggtgg ctgaagctcc ggccgccttg gcttttcttg aggcctttgc
     2821 tgccagtgtt ctggagcctc gagaacatgc attgctcacc ctgttgttgg tctatgggcc
     2881 ccgggaaggc cgagggggcc ctgacccatt tctcggagtg aaggctgcag cagctgagtt
     2941 agaacgacgg tacccagggg cgaggctggc ctggctcgct gtgcgagcag aggccccttc
     3001 gcaggtgcga ctcatggatg tcatctccaa gaaacaccct gtggacacac tcttcttcct
     3061 taccaccgtg tggacaagac ctgggtcgga agtcctcaac cgctgccgta tgaatgcaat
     3121 ctcgggctgg caggccttct ttccggtcca ctttcaggag ttcaaccctg ctctgtcacc
     3181 acagagatct cccccaggtg tccctggggc tgatcccccc tcccctggtg tggacccttc
     3241 taggggggct ccggtcgggg gcagatttga ccggcaggct tcagcagagg gctgcttcta
     3301 caatgctgac tacctggcag cccgcgcccg gctggctggc gaactggcag gccaggaaga
     3361 ggaggaagcc ctggaggggc tggaggtgat ggatgttttc ctccggttct cagggctcca
     3421 cctctttcga gccgtagagc cagggctggt gcagaagttc tctctgcggg actgtagccc
     3481 ccggctcagc gaggaactgt accaccgttg tcgcctcagc aatctggagg ggctgggggc
     3541 ccgtacccag ctagccatgg ctctgtttga acaggaacag gccaacagca cttagcctct
     3601 ccacccctgt atctgccata gccccgggaa gggcaaggca aggtgacgga cagatggagg
     3661 gcttgccact gtattttttt aaaaataaga aatgttatta aacgtatctt ctgccaaaaa
     3721 aaaaaaaaaa
//
