LOCUS       NM_001163640            3150 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus chimerin 2 (Chn2), transcript variant 2, mRNA.
ACCESSION   NM_001163640
VERSION     NM_001163640.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Su,Y.T., Lau,S.F., Ip,J.P.K., Cheung,K., Cheung,T.H.T., Fu,A.K.Y.
            and Ip,N.Y.
  TITLE     alpha2-Chimaerin is essential for neural stem cell homeostasis in
            mouse adult neurogenesis
  JOURNAL   Proc Natl Acad Sci U S A 116 (27), 13651-13660 (2019)
   PUBMED   31209021
  REMARK    GeneRIF: essential for neural stem cell homeostasis in mouse adult
            neurogenesis
REFERENCE   3  (bases 1 to 3150)
  AUTHORS   Estep,J.A., Wong,W., Wong,Y.E., Loui,B.M. and Riccomagno,M.M.
  TITLE     The RacGAP beta-Chimaerin is essential for cerebellar granule cell
            migration
  JOURNAL   Sci Rep 8 (1), 680 (2018)
   PUBMED   29330522
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3150)
  AUTHORS   Wang,Q., Chiu,S.L., Koropouli,E., Hong,I., Mitchell,S.,
            Easwaran,T.P., Hamilton,N.R., Gustina,A.S., Zhu,Q., Ginty,D.D.,
            Huganir,R.L. and Kolodkin,A.L.
  TITLE     Neuropilin-2/PlexinA3 Receptors Associate with GluA1 and Mediate
            Sema3F-Dependent Homeostatic Scaling in Cortical Neurons
  JOURNAL   Neuron 96 (5), 1084-1098 (2017)
   PUBMED   29154130
REFERENCE   5  (bases 1 to 3150)
  AUTHORS   Casado-Medrano,V., Barrio-Real,L., Garcia-Rostan,G., Baumann,M.,
            Rocks,O. and Caloca,M.J.
  TITLE     A new role of the Rac-GAP beta2-chimaerin in cell adhesion reveals
            opposite functions in breast cancer initiation and tumor
            progression
  JOURNAL   Oncotarget 7 (19), 28301-28319 (2016)
   PUBMED   27058424
  REMARK    GeneRIF: our data redefine the role of beta2-chimaerin as tumor
            suppressor and provide the first in vivo evidence of a dual
            function in breast cancer, suppressing tumor initiation but
            favoring tumor progression.
REFERENCE   6  (bases 1 to 3150)
  AUTHORS   Griner,E.M., Caino,M.C., Sosa,M.S., Colon-Gonzalez,F.,
            Chalmers,M.J., Mischak,H. and Kazanietz,M.G.
  TITLE     A novel cross-talk in diacylglycerol signaling: the Rac-GAP
            beta2-chimaerin is negatively regulated by protein kinase
            Cdelta-mediated phosphorylation
  JOURNAL   J Biol Chem 285 (22), 16931-16941 (2010)
   PUBMED   20335173
  REMARK    GeneRIF: the Rac-GAP beta2-chimaerin is negatively regulated by
            protein kinase Cdelta-mediated phosphorylation
REFERENCE   7  (bases 1 to 3150)
  AUTHORS   Takeuchi,S., Yamaki,N., Iwasato,T., Negishi,M. and Katoh,H.
  TITLE     Beta2-chimaerin binds to EphA receptors and regulates cell
            migration
  JOURNAL   FEBS Lett 583 (8), 1237-1242 (2009)
   PUBMED   19306875
REFERENCE   8  (bases 1 to 3150)
  AUTHORS   Beg,A.A., Sommer,J.E., Martin,J.H. and Scheiffele,P.
  TITLE     alpha2-Chimaerin is an essential EphA4 effector in the assembly of
            neuronal locomotor circuits
  JOURNAL   Neuron 55 (5), 768-778 (2007)
   PUBMED   17785183
REFERENCE   9  (bases 1 to 3150)
  AUTHORS   Wegmeyer,H., Egea,J., Rabe,N., Gezelius,H., Filosa,A., Enjin,A.,
            Varoqueaux,F., Deininger,K., Schnutgen,F., Brose,N., Klein,R.,
            Kullander,K. and Betz,A.
  TITLE     EphA4-dependent axon guidance is mediated by the RacGAP
            alpha2-chimaerin
  JOURNAL   Neuron 55 (5), 756-767 (2007)
   PUBMED   17785182
REFERENCE   10 (bases 1 to 3150)
  AUTHORS   Yasuda,S., Kai,M., Imai,S., Kanoh,H. and Sakane,F.
  TITLE     Diacylglycerol kinase gamma interacts with and activates
            beta2-chimaerin, a Rac-specific GAP, in response to epidermal
            growth factor
  JOURNAL   FEBS Lett 581 (3), 551-557 (2007)
   PUBMED   17254573
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB617538.1, AK131641.1, BM935649.1, BQ175659.1 and BB676245.1.
            
            Transcript Variant: This variant (2) uses an alternate, upstream
            transcription initiation site, uses a distinct start codon and
            contains several additional exons, compared to variant 1. The
            resulting protein (isoform 2, also referred to as beta2-chimerin)
            has a much longer and distinct N-terminus compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.105175.1,
                                           SRR1660817.146645.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-107               BB617538.1         2-108
            108-2059            AK131641.1         2-1953
            2060-2484           BM935649.1         87-511
            2485-3146           BQ175659.1         18-679              c
            3147-3150           BB676245.1         403-406
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 26.54 cM"
     gene            1..3150
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /note="chimerin 2"
                     /db_xref="GeneID:69993"
                     /db_xref="MGI:MGI:1917243"
     exon            1..224
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     CDS             176..1582
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /note="isoform 2 is encoded by transcript variant 2;
                     beta-chimaerin; Rho GTPase-activating protein 3; chimerin
                     (chimaerin) 2"
                     /codon_start=1
                     /product="beta-chimaerin isoform 2"
                     /protein_id="NP_001157112.1"
                     /db_xref="CCDS:CCDS51778.1"
                     /db_xref="GeneID:69993"
                     /db_xref="MGI:MGI:1917243"
                     /translation="MAASSNSSLSGSSVSSDAEEYQPPIWKSYLYQLQQEAPRPKRII
                     CPREVENRPKYYGREFHGIISREQADELLGGVEGAYILRESQRQPGCYTLALRFGNQT
                     LNYRLFHDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYIAKMTTNPIYEHIGYAT
                     LLREKVSRRLSRSKNESRKASVSNEEHTPVEKISSLVRRAALTHNDNHFNYEKTHNFK
                     VHTFRGPHWCEYCANFMWGLIAQGVRCSDCGLNVHKQCSKHVPNDCQPDLKRIKKVYC
                     CDLTTLVKAHNTQRPMVVDICIREIEARGLKSEGLYRVSGFTEHIEDVKMAFDRDGEK
                     ADISANIYPDINIITGALKLYFRDLPIPIITYDTYSKFIEAAKISNADERLEAVHEVL
                     MLLPPAHYETLRYLMIHLKKVTMNEKDNLMNAENLGIVFGPTLMRPPEDSTLTTLHDM
                     RYQKLIVQILIENEDVLF"
     exon            225..263
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            264..319
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            320..351
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            352..465
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            466..751
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            752..829
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            830..914
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1088
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            1089..1166
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1304
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1410
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..3150
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1616..1621
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /note="hexamer: AATAAA"
     polyA_site      1634
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /note="major polyA site"
     regulatory      3127..3132
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
                     /note="hexamer: ATTAAA"
     polyA_site      3146
                     /gene="Chn2"
                     /gene_synonym="1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch"
ORIGIN      
        1 agcgcgtcat ctggcggagc aggaagtaca ggcagagtcc ggaggccggt gcttcccgcg
       61 cgtccccagg actttgccat gggctggggg ccgcgcgggc tgcgagcagc cggccgaggg
      121 cagcggcgac gccgcccgca ccaggactga gagagcggtg acgcgcggcg ccgagatggc
      181 agcatccagc aactctagtc tgtccggctc ctccgtgtcc tctgatgctg aggaatacca
      241 gcctccaata tggaaatcat acttgtatca gctgcagcaa gaggcacctc gtcccaagag
      301 gatcatttgt ccccgggagg tggaaaacag accaaaatat tatggaagag agtttcatgg
      361 gatcatctct cgggagcagg ctgatgagct tctaggcggc gtggaaggtg cctacatcct
      421 tcgagaaagc cagcggcagc caggatgcta cacgctggca ctgaggtttg ggaaccagac
      481 cttaaactac cggctcttcc acgacgggaa gcactttgtg ggtgagaaga ggttcgaatc
      541 cattcacgac ctggtgacag atggcttgat cacactctac atagagacca aggctgctga
      601 gtacatcgcg aaaatgacca caaatcccat ctacgagcac atcggatacg ccactctcct
      661 cagagaaaaa gtgtcccgaa ggctgagcag gtctaaaaac gaatcaagga aagctagcgt
      721 cagcaacgag gagcacacgc cagtggagaa gatctcttca ctggtccgaa gagctgccct
      781 cacacacaac gacaaccact tcaactatga gaagacacac aacttcaagg tgcacacatt
      841 ccggggccca cactggtgtg aatactgtgc caattttatg tgggggctca tcgcccaggg
      901 agtccggtgt tcagattgtg ggttaaacgt acacaaacag tgctccaagc acgtccccaa
      961 tgactgccag ccagatctca agaggatcaa gaaagtgtac tgctgtgacc tcaccacgct
     1021 tgtcaaagct cacaacactc agagacccat ggtggtggac atatgtattc gggaaattga
     1081 agcaagagga ttaaagtcag aagggcttta cagagtctct ggattcacag aacacattga
     1141 agatgtcaag atggcgtttg acagagatgg tgaaaaggcg gatatctctg ccaacatcta
     1201 cccagacata aacatcatca ctggagccct gaaactgtac ttccgagact tacccatccc
     1261 tatcatcaca tatgacacct actccaaatt tatagaggca gcaaaaatct ccaatgcgga
     1321 tgagaggctg gaagctgtcc atgaggtcct gatgctgctg cctcctgccc actatgagac
     1381 cctgcgctac ctgatgatcc acctgaaaaa ggttaccatg aatgaaaagg acaatttaat
     1441 gaatgccgaa aatctgggga tcgtgtttgg gcccaccctg atgagacccc ctgaggacag
     1501 caccctcacc accctccatg acatgcggta ccaaaagctg atcgtgcaga ttttaataga
     1561 aaacgaagat gttctgtttt aatccaccag ggaagtgagc tgatagagct agtggaataa
     1621 aaacgtttct cacacttgat ttgtttattc aaacaagtgg cagaatttcc tggaccacag
     1681 tggatcgcag agttggggac tgtgtctccc gcctccacgt gagagcaagg gtgagggtag
     1741 gaaacccctt accttgggtc ttttgccgtg cctcgtatgt atgtctgttt tgctggaaga
     1801 gtgattaata aatctttctt taacttatta aaaaaaaaaa ctagactttt aagcttcggt
     1861 cttaacagta ataaaaggga acttagttca taaaggtact tggtacagta tgcatttccc
     1921 accttcaaaa agagaagaca attctatatg ttaaatgtta aatgaaacct atattgtaag
     1981 atgtatttta ttttagctgc aagaatgtta ttttatttta gcaaatagaa tccaatgcag
     2041 tgcgttggtt atggccctgt atcttgtccc cttcttgctg tgatgcctgg tgaacgttga
     2101 ccgtgaatgc agtgtcacct tgacatacct ctgtcccgat gagttcttcc atgagagttc
     2161 acacctcctc gtgcccctgc ttttcacagc tcctgctcct cagcactggc ttttctgctg
     2221 tgagatagtg tatgttcata gtgtttctag gctggtctga agcatctgcc aatttccttt
     2281 ctgtgggctt tttgaggctt cccaaaggat gttatggaat tagtcatggc ctctccatat
     2341 ctcccgtgta catcctatta cgtgcagtgt gcaccgctac acaaaatgac ttgaggacac
     2401 tggatgcagc tggacggtgt cacagaatta gcactttccc tctcctcagc caggcccttg
     2461 agactgatgc caatggcaag ttgaaattct ttgccgcatt tccatatgga tgaatcatga
     2521 accttgaagg atcattgttg aatcttgttc ctttctttca ttttataaac caattattaa
     2581 attatgaata gataaggttg gtatctttta atcaaatgtt tttccttcat aaaagttgct
     2641 ctgtatggaa gataatttaa cataatttta ttactagaaa cacatcctgt cggttatatt
     2701 tatcttagca tgagatttcc acaccaagat ctctatattt tgtaacatag gcaaaaatat
     2761 ttaaaacagc aaaaactgta catttagatt tttaaagcca gtgactttcc cttgattgtc
     2821 tgagatgacc cacacactga caagtcttgc ttagagtgcc cagaagaaaa ctgccctgtt
     2881 acagtgatag agactgcaga agtgctctat tagcctcccc aggccctgca ggcagaatcg
     2941 tggcatctgg ggctcagttt ggcttgctct ctgctgtcac tgtccctcat gactgtacag
     3001 atttgtttgt tgtggcttct gttcttcttg atactgtcaa taataatctg gaaatggttt
     3061 tattttgtga agtgagcaat actttgtaat ttatgacagt gtaatggaag gaaaaccatt
     3121 ccatgtatta aatactctgt ccatcatttt
//
