LOCUS       NM_025783               2615 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus charged multivesicular body protein 3 (Chmp3),
            transcript variant 1, mRNA.
ACCESSION   NM_025783 XM_973747
VERSION     NM_025783.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Wollert,T., Wunder,C., Lippincott-Schwartz,J. and Hurley,J.H.
  TITLE     Membrane scission by the ESCRT-III complex
  JOURNAL   Nature 458 (7235), 172-177 (2009)
   PUBMED   19234443
REFERENCE   3  (bases 1 to 2615)
  AUTHORS   Azmi,I., Davies,B., Dimaano,C., Payne,J., Eckert,D., Babst,M. and
            Katzmann,D.J.
  TITLE     Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a
            conserved VSL region in Vta1
  JOURNAL   J Cell Biol 172 (5), 705-717 (2006)
   PUBMED   16505166
REFERENCE   4  (bases 1 to 2615)
  AUTHORS   Diaz,E., Yang,Y.H., Ferreira,T., Loh,K.C., Okazaki,Y.,
            Hayashizaki,Y., Tessier-Lavigne,M., Speed,T.P. and Ngai,J.
  TITLE     Analysis of gene expression in the developing mouse retina
  JOURNAL   Proc Natl Acad Sci U S A 100 (9), 5491-5496 (2003)
   PUBMED   12702772
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY264596.1, AK083562.1 and AI848675.1.
            
            On Mar 19, 2018 this sequence version replaced NM_025783.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083562.1, BC049964.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 BY264596.1         1-2
            3-2594              AK083562.1         2-2593
            2595-2615           AI848675.1         1-21                c
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 32.17 cM"
     gene            1..2615
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /note="charged multivesicular body protein 3"
                     /db_xref="GeneID:66700"
                     /db_xref="MGI:MGI:1913950"
     exon            1..182
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..812
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neuroendocrine differentiation factor; chromatin-modifying
                     protein 3; vacuolar protein sorting-associated protein 24"
                     /codon_start=1
                     /product="charged multivesicular body protein 3 isoform 1"
                     /protein_id="NP_080059.2"
                     /db_xref="CCDS:CCDS20232.1"
                     /db_xref="GeneID:66700"
                     /db_xref="MGI:MGI:1913950"
                     /translation="MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKV
                     KRSVKDAAKKGQKEVCVVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAG
                     SLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEA
                     AEMEIDRILFEITAGALGKAPSKVTDALPEPEPAGAMAASEEGEEEEDEEDLEAMQSR
                     LATLRS"
     exon            183..243
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /inference="alignment:Splign:2.1.0"
     exon            244..423
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /inference="alignment:Splign:2.1.0"
     exon            424..545
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /inference="alignment:Splign:2.1.0"
     exon            546..660
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /inference="alignment:Splign:2.1.0"
     exon            661..2599
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1167..1172
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /note="hexamer: AATAAA"
     polyA_site      1191
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /note="major polyA site"
     regulatory      2577..2582
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /note="hexamer: AATAAA"
     polyA_site      2599
                     /gene="Chmp3"
                     /gene_synonym="25.1; 4921505F14Rik; 9130011K15Rik; CGI-49;
                     D6Ertd286e; NEDF; Vps24"
                     /note="major polyA site"
ORIGIN      
        1 gtactagccc tgctgccgcc gcgcccttct ctgggcgtct gattcccttc ctcccagccc
       61 cgcccaggcg gctgcccgtg acctgcctgg gcgcagggaa cggaaagtcg gaaggggtaa
      121 gaggagttca gttcatcatg ggactctttg gaaaaaccca ggagaagcct cccaaagagc
      181 tggttaatga atggtcactg aagatcagaa aggaaatgag agttgttgac aggcaaataa
      241 gagatatcca aagagaagaa gagaaagtaa aacggtctgt gaaagatgca gccaagaagg
      301 gccaaaagga agtctgtgtg gttctggcca aggagatgat caggtcaagg aaagccgtga
      361 gcaagctcta tgcctccaaa gcacacatga actctgtgct catgggcatg aagaaccaac
      421 tcgcggtcct gcgagtagct ggttctctgc agaagagcac agaagtgatg aaggcaatgc
      481 agagtcttgt gaagatccca gaaatccagg ccaccatgcg ggagctgtcc aaagagatga
      541 tgaaggctgg aatcatagag gagatgttag aggatacatt tgaaagcatg gatgatcaag
      601 aagaaatgga agaagcagct gagatggaga ttgacagaat cctctttgaa atcacagcag
      661 gagccttggg caaagcaccc agtaaagtaa ccgatgccct tcctgagcct gaacccgcag
      721 gagcgatggc tgcctccgaa gagggagaag aggaagaaga tgaagaggac ctggaggcta
      781 tgcagtcacg gctggccaca ctcagaagct agggccaccc agttgggggt ctcaactctc
      841 tggtgtcctc ctctaggtgt gtatctggcc tctgaggagt caccatttct tgtatctctt
      901 gcactacacc tctgctgtga agcattacat ttggagaggg ttctgtgctg gtatatcttc
      961 acgatctgca gaggatttaa ggattgcttt tatgaaggtg gtgtaatcaa atgtattatt
     1021 ttcaggagca taaatagcag aagtatcttc cttgggggag ggcaaagaaa tccattttct
     1081 catgaagcaa ttttgagaag agtagattga atgatgagga ctctatattt ggtgtgtatg
     1141 tgtaaaataa tatattaatg gatgtcaata aacttctgct ttagctcttg gtctcattgt
     1201 aaatgtcaca tgcaatgaac tcaagatatt aatctcccag attgtctcag tgttggcctt
     1261 ggaaagagga catgctatct ccaagcatcg ggacatggga gcttagcaga agtaccttca
     1321 gctccggggc cgctggtcct gctcttcaca ggctggttgt gggggccact cttgtgcatt
     1381 ttgtcatttg tctaaccttc gggttaacgt gctgtgttga ctgaatgacc atatcataca
     1441 ctggtgtgta caagggagtt tttacagctg gtaggcattc ttccccactg caagtgaaat
     1501 gcaggacagt tagatctggg atcaggactc ttgcagtcca tccctgcttg gagctaagtg
     1561 tcttgtaccc agggacgtag ccttaaccat gagcaggaag agctaaccag gaagaggttt
     1621 ggattatgca gtgaaacaca cacagcagag catgctttag atgcattgag aaatagccca
     1681 gcagtagcac tgtgtggagc agtttgaaaa gctttctggt atgcatgatc ttcacaaatg
     1741 aggacagctg tcatctgaag tcacaaaccc taaagtacaa tagccagtat ccgccagtat
     1801 tgtacagaat ggctttgtta cccaaaacac aacccatgtg ctgttcattg cttcctctaa
     1861 tcccttggca cccactgacc cggttgctgt ctccatgggc tttcctttcc cagcctttca
     1921 gtatgtcttc cctcacgtgg tgatatgcat ttacgttcct ccgtggattc atgacttgac
     1981 tgttcagccc ttctcattga gcagcgttcc attttctagc tgtaccacag cttgtcttga
     2041 aggccacctg gactgcttct gagtttggga gtttgaagca atgtctgtag caggtttccc
     2101 tgtgtgtttt aattcctctg gataccagag agctcaaccc tgggatcatg tggtcagcac
     2161 aaacctgtgc tgtaaggtaa accagccgtg cagagcacgt actgtccccg tctcaggagg
     2221 aagagttctg cctgccatcc tgtggctttg gcagaatttg tcatgaggga tttgtgttga
     2281 agtaccctgg gcatgttttt atacttgcct tctctgtatc ttgtttgaaa atgttgacat
     2341 agtggccttg aactcatgac ctgtctgctc taccaccaca ctgagcttgc cgcctggatt
     2401 tcttcgttgt tggtgtttgt tcattttata acctggattg tttgttttct gattattcaa
     2461 gtttaaggac ctcttacaga ttttgaacca atcatctatc agattatatc ctattctctg
     2521 ttctattctc tcattgttga ctgtttttca aagctgaagg tttctttttt acttttaata
     2581 aaaccctgct tctcaattaa aaaaaaaaaa aaaaa
//
