LOCUS       NP_031718                394 aa            linear   ROD 28-APR-2025
DEFINITION  choline/ethanolamine kinase [Mus musculus].
ACCESSION   NP_031718
VERSION     NP_031718.1
DBSOURCE    REFSEQ: accession NM_007692.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 394)
  AUTHORS   Tavasoli,M. and McMaster,C.R.
  TITLE     Defects in integrin complex formation promote CHKB-mediated
            muscular dystrophy
  JOURNAL   Life Sci Alliance 7 (8), e202301956 (2024)
   PUBMED   38749543
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 394)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 394)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 394)
  AUTHORS   Kular,J., Tickner,J.C., Pavlos,N.J., Viola,H.M., Abel,T., Lim,B.S.,
            Yang,X., Chen,H., Cook,R., Hool,L.C., Zheng,M.H. and Xu,J.
  TITLE     Choline kinase beta mutant mice exhibit reduced phosphocholine,
            elevated osteoclast activity, and low bone mass
  JOURNAL   J Biol Chem 290 (3), 1729-1742 (2015)
   PUBMED   25451916
  REMARK    GeneRIF: CHKB is a new modulator of bone homeostasis
REFERENCE   5  (residues 1 to 394)
  AUTHORS   Li,Z., Wu,G., Sher,R.B., Khavandgar,Z., Hermansson,M., Cox,G.A.,
            Doschak,M.R., Murshed,M., Beier,F. and Vance,D.E.
  TITLE     Choline kinase beta is required for normal endochondral bone
            formation
  JOURNAL   Biochim Biophys Acta 1840 (7), 2112-2122 (2014)
   PUBMED   24637075
  REMARK    GeneRIF: Our data indicate that choline kinase beta plays an
            important role in endochondral bone formation by modulating growth
            plate physiology.
REFERENCE   6  (residues 1 to 394)
  AUTHORS   Yamazaki,N., Yamanaka,Y., Hashimoto,Y., Hiramatsu,T., Shinohara,Y.
            and Terada,H.
  TITLE     The gene encoding muscle-type carnitine palmitoyltransferase I:
            comparison of the 5'-upstream region of human and rodent genes
  JOURNAL   J Biochem 133 (4), 523-532 (2003)
   PUBMED   12761301
REFERENCE   7  (residues 1 to 394)
  AUTHORS   van der Leij,F.R., Cox,K.B., Jackson,V.N., Huijkman,N.C.,
            Bartelds,B., Kuipers,J.R., Dijkhuizen,T., Terpstra,P., Wood,P.A.,
            Zammit,V.A. and Price,N.T.
  TITLE     Structural and functional genomics of the CPT1B gene for
            muscle-type carnitine palmitoyltransferase I in mammals
  JOURNAL   J Biol Chem 277 (30), 26994-27005 (2002)
   PUBMED   12015320
REFERENCE   8  (residues 1 to 394)
  AUTHORS   Aoyama,C., Ohtani,A. and Ishidate,K.
  TITLE     Expression and characterization of the active molecular forms of
            choline/ethanolamine kinase-alpha and -beta in mouse tissues,
            including carbon tetrachloride-induced liver
  JOURNAL   Biochem J 363 (Pt 3), 777-784 (2002)
   PUBMED   11964179
  REMARK    GeneRIF: Experimental evidence for the presence of heteromeric
            ChoK/EtnK in any source. The activity of ChoK/EtnK in the cell is
            controlled not only by the level of each isoform but also by their
            combination to form the active oligomer complex.
REFERENCE   9  (residues 1 to 394)
  AUTHORS   Aoyama,C., Yamazaki,N., Terada,H. and Ishidate,K.
  TITLE     Structure and characterization of the genes for murine
            choline/ethanolamine kinase isozymes alpha and beta
  JOURNAL   J Lipid Res 41 (3), 452-464 (2000)
   PUBMED   10706593
REFERENCE   10 (residues 1 to 394)
  AUTHORS   Aoyama,C., Nakashima,K. and Ishidate,K.
  TITLE     Molecular cloning of mouse choline kinase and choline/ethanolamine
            kinase: their sequence comparison to the respective rat homologs
  JOURNAL   Biochim Biophys Acta 1393 (1), 179-185 (1998)
   PUBMED   9714798
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK078101.1, AC137513.3 and AI853330.1.
            
            Transcript Variant: This variant (1) represents the shortest
            transcript but encodes the supported protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK078101.1, AB011000.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 44.89 cM"
     Protein         1..394
                     /product="choline/ethanolamine kinase"
                     /EC_number="2.7.1.32"
                     /EC_number="2.7.1.82"
                     /note="choline/ethanolaminekinase; choline/ethanolamine
                     kinase-b"
                     /calculated_mol_wt=44995
     Region          1..42
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O55229.3)"
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylalanine.
                     /evidence=ECO:0000250|UniProtKB:Q9Y259; propagated from
                     UniProtKB/Swiss-Prot (O55229.3)"
     Region          73..385
                     /region_name="ChoK_euk"
                     /note="Euykaryotic Choline Kinase; cd05156"
                     /db_xref="CDD:270705"
     Site            order(75,77,79..80,82,102,104,133,145..148,150..152,242,
                     244,246..247,249,263..264,267,283,288,353,356)
                     /site_type="active"
                     /db_xref="CDD:270705"
     Site            order(75,80,82,102,104,133,145..148,150..152,246,249,
                     263..264)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270705"
     Site            order(77,79,242,244,247,267,283,288,353,356)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:270705"
     Site            order(117,120..121,124..125,128..129,135..138,180..181,
                     183..184)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270705"
     CDS             1..394
                     /gene="Chkb"
                     /gene_synonym="Chetk; Chkl; Ck; Ck/Ek; Ck/Ek-beta; Ckb;
                     Ckekb; Ek"
                     /coded_by="NM_007692.6:328..1512"
                     /db_xref="CCDS:CCDS27750.1"
                     /db_xref="GeneID:12651"
                     /db_xref="MGI:MGI:1328313"
ORIGIN      
        1 maadgtgvvg ggavggglpk dglqdakcpe pipnrrrass lsrdaqrray qwcreylgga
       61 wrrarpeels vcpvsgglsn llfrcslpnh vpsvggepre vllrlygail qgvdslvles
      121 vmfailaers lgpqlygvfp egrleqylps rplktqelrd pvlsgaiatr marfhgmemp
      181 ftkeprwlfg tmerylkqiq dlpstslpqm nlvemyslkd emnslrklld dtpspvvfch
      241 ndiqegnill lsepdsddnl mlvdfeyssy nyrgfdignh fcewvydyty eewpfykarp
      301 tdyptreqql hfirhylaev qkgeilseee qkkreeelll eisryslash ffwglwstlq
      361 asmstiefgy leyaqsrfqf yfqqkgqlts spss
//
