LOCUS       NM_001410133            2596 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus choline kinase alpha (Chka), transcript variant 5,
            mRNA.
ACCESSION   NM_001410133 XM_006531646
VERSION     NM_001410133.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2596)
  AUTHORS   Butterfield,N.C., Curry,K.F., Steinberg,J., Dewhurst,H.,
            Komla-Ebri,D., Mannan,N.S., Adoum,A.T., Leitch,V.D., Logan,J.G.,
            Waung,J.A., Ghirardello,E., Southam,L., Youlten,S.E.,
            Wilkinson,J.M., McAninch,E.A., Vancollie,V.E., Kussy,F.,
            White,J.K., Lelliott,C.J., Adams,D.J., Jacques,R., Bianco,A.C.,
            Boyde,A., Zeggini,E., Croucher,P.I., Williams,G.R. and
            Bassett,J.H.D.
  TITLE     Accelerating functional gene discovery in osteoarthritis
  JOURNAL   Nat Commun 12 (1), 467 (2021)
   PUBMED   33473114
  REMARK    Erratum:[Nat Commun. 2021 May 28;12(1):3302. doi:
            10.1038/s41467-021-23768-8.. PMID: 34050183]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2596)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2596)
  AUTHORS   Grechez-Cassiau,A., Feillet,C., Guerin,S. and Delaunay,F.
  TITLE     The hepatic circadian clock regulates the choline kinase alpha gene
            through the BMAL1-REV-ERBalpha axis
  JOURNAL   Chronobiol Int 32 (6), 774-784 (2015)
   PUBMED   26125130
  REMARK    GeneRIF: hepatic phosphatidylcholine is regulated by the circadian
            clock through a Bmal1-Rev-erbalpha-Chkalpha axis
REFERENCE   6  (bases 1 to 2596)
  AUTHORS   Sher,R.B., Aoyama,C., Huebsch,K.A., Ji,S., Kerner,J., Yang,Y.,
            Frankel,W.N., Hoppel,C.L., Wood,P.A., Vance,D.E. and Cox,G.A.
  TITLE     A rostrocaudal muscular dystrophy caused by a defect in choline
            kinase beta, the first enzyme in phosphatidylcholine biosynthesis
  JOURNAL   J Biol Chem 281 (8), 4938-4948 (2006)
   PUBMED   16371353
REFERENCE   7  (bases 1 to 2596)
  AUTHORS   Liao,H., Aoyama,C., Ishidate,K. and Teraoka,H.
  TITLE     Deletion and alanine mutation analyses for the formation of active
            homo- or hetero-dimer complexes of mouse choline kinase-alpha and
            -beta
  JOURNAL   Biochim Biophys Acta 1761 (1), 111-120 (2006)
   PUBMED   16490392
  REMARK    GeneRIF: Full-length cDNAs or their truncated or alanine-mutated
            versions for mouse CK-alpha1 and CK-beta were expressed in COS-7
            cells.
REFERENCE   8  (bases 1 to 2596)
  AUTHORS   Aoyama,C., Ohtani,A. and Ishidate,K.
  TITLE     Expression and characterization of the active molecular forms of
            choline/ethanolamine kinase-alpha and -beta in mouse tissues,
            including carbon tetrachloride-induced liver
  JOURNAL   Biochem J 363 (Pt 3), 777-784 (2002)
   PUBMED   11964179
  REMARK    GeneRIF: Experimental evidence for the presence of heteromeric
            ChoK/EtnK in any source. The activity of ChoK/EtnK in the cell is
            controlled not only by the level of each isoform but also by their
            combination to form the active oligomer complex.
REFERENCE   9  (bases 1 to 2596)
  AUTHORS   Aoyama,C., Yamazaki,N., Terada,H. and Ishidate,K.
  TITLE     Structure and characterization of the genes for murine
            choline/ethanolamine kinase isozymes alpha and beta
  JOURNAL   J Lipid Res 41 (3), 452-464 (2000)
   PUBMED   10706593
REFERENCE   10 (bases 1 to 2596)
  AUTHORS   Aoyama,C., Nakashima,K. and Ishidate,K.
  TITLE     Molecular cloning of mouse choline kinase and choline/ethanolamine
            kinase: their sequence comparison to the respective rat homologs
  JOURNAL   Biochim Biophys Acta 1393 (1), 179-185 (1998)
   PUBMED   9714798
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC133523.3.
            
            On Jul 27, 2022 this sequence version replaced XM_006531646.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.3908800.1,
                                           SRR13422597.9545.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-582               AC133523.3         88713-89294
            583-694             AC133523.3         101529-101640
            695-724             AC133523.3         111650-111679
            725-778             AC133523.3         112616-112669
            779-892             AC133523.3         121353-121466
            893-1026            AC133523.3         122741-122874
            1027-1131           AC133523.3         123243-123347
            1132-1190           AC133523.3         124270-124328
            1191-1278           AC133523.3         125904-125991
            1279-1387           AC133523.3         126761-126869
            1388-1494           AC133523.3         128988-129094
            1495-1576           AC133523.3         129591-129672
            1577-2596           AC133523.3         130229-131248
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 3.57 cM"
     gene            1..2596
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /note="choline kinase alpha"
                     /db_xref="GeneID:12660"
                     /db_xref="MGI:MGI:107760"
     exon            1..582
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             245..1636
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /EC_number="2.7.1.32"
                     /EC_number="2.7.1.82"
                     /note="isoform 4 is encoded by transcript variant 5;
                     ethanolamine kinase; choline/ethanolamine kinase alpha"
                     /codon_start=1
                     /product="choline kinase alpha isoform 4"
                     /protein_id="NP_001397062.1"
                     /db_xref="GeneID:12660"
                     /db_xref="MGI:MGI:107760"
                     /translation="MKTKFCTGGEAEPSPLGLLLSCGGNAAPTPGVGQQRDAAGELES
                     KQLGGRTQPLALPPPPPPPLPLPPPPSPPLADEQPEPRTRRRAYLWCKEFLPGAWRGL
                     REDQFHISVIRGGLSNMLFQCSLPDSIASVGDEPRKVLLRLYGAILKMVFLIKLWNGK
                     RSCNKEGSEQAQNENEFQGAEAMVLESVMFAILAERSLGPKLFGIFPQGRLEQFIPSR
                     RLDTEELRLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLNQVLRLKFSREAR
                     VQQLHKILSYNLPLELENLRSLLQYTRSPVVFCHNDCQEGNILLLEGQENSERRKLML
                     IDFEYSSYNYRGFDIGNHFCEWMYDYTYEKYPFFRANIQKYPSRKQQLHFISSYLTTF
                     QNDFESLSSEEQFATKEDMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMEYAQAR
                     FEAYFDQKRKLGV"
     exon            583..694
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            695..724
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            725..778
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            779..892
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1026
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1131
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1190
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1278
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1387
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1494
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1576
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1577..2596
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2577..2582
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /note="hexamer: AATAAA"
     polyA_site      2596
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /note="major polyA site"
ORIGIN      
        1 agcagtcgcc tgccgtcagc ctcccgcgct cgtctctcgt tactgctgcc tgacgtccat
       61 tgctgcctct ccccgccgtc gccgacgtcg cctccccgcg cgctcccaca accgccgccc
      121 ggccggtcag tgaagccggc gagccattcc ccgcgccggc ccccagaggc gggcatccag
      181 ccggaccctg agtgtggccc tctcgtgctg tggccgcccg cgccttctcg gccgcttgtc
      241 cagcatgaaa accaagttct gcaccggggg cgaggccgag ccgtcgcctc tcgggctgct
      301 gctgagctgc ggtggcaacg ctgccccgac gcccggcgta gggcagcagc gggacgccgc
      361 aggcgagctg gagtccaagc agcttggtgg ccgaacccaa cctctcgcgc tgccgccgcc
      421 gccgccgccg cccctgccgc tgcccccgcc gccatcaccg ccgctagcgg acgaacaacc
      481 cgagccccgg accaggcgca gggcctacct gtggtgtaag gaattcctgc ccggagcctg
      541 gcgaggcctt cgcgaggacc agttccacat cagtgtcatc aggggtggtc tcagtaacat
      601 gctgttccag tgctccctgc cagactccat agccagcgtt ggcgatgagc ctcggaaagt
      661 gctcttgcgg ctctatgggg caatcttaaa gatggtgttt ttgataaagc tgtggaatgg
      721 caagaggtcc tgtaataaag agggatccga acaagctcag aatgaaaatg aatttcaagg
      781 ggctgaggcg atggttctgg agagtgttat gtttgccatt cttgcagaga ggtcacttgg
      841 gccaaaactc tttggcatct ttccccaagg ccgactggag cagtttatcc cgagccggcg
      901 attggacact gaagagttac gtttaccaga tatttctgca gaaatagctg agaaaatggc
      961 cacatttcat ggtatgaaaa tgccattcaa taaggaacca aaatggcttt ttggaacaat
     1021 ggaaaaatac ctgaatcaag tactaagact caaattcagc agggaggcca gagttcagca
     1081 actgcacaag atcctctctt acaacctgcc tcttgagctc gagaacctga ggtcattgct
     1141 gcagtatact agatctccag ttgtattttg tcataatgac tgtcaagaag gtaatatctt
     1201 attgttggaa ggccaggaga actctgaaag gcggaagctg atgcttattg actttgagta
     1261 cagcagttac aattacaggg gatttgacat tggaaatcat ttctgtgaat ggatgtatga
     1321 ttatacctat gagaagtatc ctttcttcag agcgaacatt cagaagtatc cttcccgaaa
     1381 acaacagctc cattttattt ccagttactt gactacattc caaaatgatt ttgaaagcct
     1441 cagtagtgaa gagcagtttg ctacaaaaga agacatgttg cttgaagtca acagatttgc
     1501 tcttgcctct catttcctct ggggactttg gtccattgta caggccaaga tctcatccat
     1561 tgaatttggg tacatggaat atgcccaagc cagattcgag gcctactttg accagaagag
     1621 gaagcttggg gtgtgaatgg ggtggctcca ttcttcacca ctggactgcg ggaggcggct
     1681 gcaccaggcc ctcagtggag cgctgctgtg accactgccc tgggcagaag gcctggatgt
     1741 ctcactactg agcaccgatg tgtatgatac tacagactat attaaagtgg agtaacattt
     1801 ctttcatctt tgtttacact ctcactagga ctctgaacca tgattggaag cagaaatata
     1861 gtacgatagt gcaatagctc agaacccgcc taaactgaag gccttttggc tgcatggcta
     1921 cagctttcag ccacttacgg ccccagctgg acagagcagt gctgtgtggg tactccaagt
     1981 gctgacttag gatattaatg tgctgcaaca catttgtcac caggctttga aggtgacagc
     2041 ctgacaatgt gttggagaca cttctgaagg gcaagtgaac agacatactg tgaagtggct
     2101 caacaggagg agcctgagtt gtggggtcta tggatggcaa ccagctgatt ctgtacaggg
     2161 tacacttgac tgtgggtatg caggtggcag ctgcagccct cctgtgcttg tgtacgcatg
     2221 actgcatgga ccagcaccac tgcctggcca taacttcagt gtctcctaac tgggtgtgct
     2281 ttatagctcc agcttcctgg gaggagcagt ggagccagct ccctcacccc ttcttgcctt
     2341 ccctctgcct ggcctggaac tcgggcttct gcccattgcc ctctgaagct gcttcccatc
     2401 tgatgtcact gggagacagc agctgtctgt ggggtattgg ggtgcaggta gattagagct
     2461 gtgaaatcca tgtacattaa tacccaatgg gataaactta gatttttttt ttactctgaa
     2521 ctctgaattg tttgtgcaca tatttctgct accaccaaga ctgtattata cagataaata
     2581 aacaacttga aacctg
//
