LOCUS       NP_001397062             463 aa            linear   ROD 01-MAY-2025
DEFINITION  choline kinase alpha isoform 4 [Mus musculus].
ACCESSION   NP_001397062 XP_006531709
VERSION     NP_001397062.1
DBSOURCE    REFSEQ: accession NM_001410133.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 463)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 463)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 463)
  AUTHORS   Butterfield,N.C., Curry,K.F., Steinberg,J., Dewhurst,H.,
            Komla-Ebri,D., Mannan,N.S., Adoum,A.T., Leitch,V.D., Logan,J.G.,
            Waung,J.A., Ghirardello,E., Southam,L., Youlten,S.E.,
            Wilkinson,J.M., McAninch,E.A., Vancollie,V.E., Kussy,F.,
            White,J.K., Lelliott,C.J., Adams,D.J., Jacques,R., Bianco,A.C.,
            Boyde,A., Zeggini,E., Croucher,P.I., Williams,G.R. and
            Bassett,J.H.D.
  TITLE     Accelerating functional gene discovery in osteoarthritis
  JOURNAL   Nat Commun 12 (1), 467 (2021)
   PUBMED   33473114
  REMARK    Erratum:[Nat Commun. 2021 May 28;12(1):3302. doi:
            10.1038/s41467-021-23768-8. PMID: 34050183]
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 463)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 463)
  AUTHORS   Grechez-Cassiau,A., Feillet,C., Guerin,S. and Delaunay,F.
  TITLE     The hepatic circadian clock regulates the choline kinase alpha gene
            through the BMAL1-REV-ERBalpha axis
  JOURNAL   Chronobiol Int 32 (6), 774-784 (2015)
   PUBMED   26125130
  REMARK    GeneRIF: hepatic phosphatidylcholine is regulated by the circadian
            clock through a Bmal1-Rev-erbalpha-Chkalpha axis
REFERENCE   6  (residues 1 to 463)
  AUTHORS   Sher,R.B., Aoyama,C., Huebsch,K.A., Ji,S., Kerner,J., Yang,Y.,
            Frankel,W.N., Hoppel,C.L., Wood,P.A., Vance,D.E. and Cox,G.A.
  TITLE     A rostrocaudal muscular dystrophy caused by a defect in choline
            kinase beta, the first enzyme in phosphatidylcholine biosynthesis
  JOURNAL   J Biol Chem 281 (8), 4938-4948 (2006)
   PUBMED   16371353
REFERENCE   7  (residues 1 to 463)
  AUTHORS   Liao,H., Aoyama,C., Ishidate,K. and Teraoka,H.
  TITLE     Deletion and alanine mutation analyses for the formation of active
            homo- or hetero-dimer complexes of mouse choline kinase-alpha and
            -beta
  JOURNAL   Biochim Biophys Acta 1761 (1), 111-120 (2006)
   PUBMED   16490392
  REMARK    GeneRIF: Full-length cDNAs or their truncated or alanine-mutated
            versions for mouse CK-alpha1 and CK-beta were expressed in COS-7
            cells.
REFERENCE   8  (residues 1 to 463)
  AUTHORS   Aoyama,C., Ohtani,A. and Ishidate,K.
  TITLE     Expression and characterization of the active molecular forms of
            choline/ethanolamine kinase-alpha and -beta in mouse tissues,
            including carbon tetrachloride-induced liver
  JOURNAL   Biochem J 363 (Pt 3), 777-784 (2002)
   PUBMED   11964179
  REMARK    GeneRIF: Experimental evidence for the presence of heteromeric
            ChoK/EtnK in any source. The activity of ChoK/EtnK in the cell is
            controlled not only by the level of each isoform but also by their
            combination to form the active oligomer complex.
REFERENCE   9  (residues 1 to 463)
  AUTHORS   Aoyama,C., Yamazaki,N., Terada,H. and Ishidate,K.
  TITLE     Structure and characterization of the genes for murine
            choline/ethanolamine kinase isozymes alpha and beta
  JOURNAL   J Lipid Res 41 (3), 452-464 (2000)
   PUBMED   10706593
REFERENCE   10 (residues 1 to 463)
  AUTHORS   Aoyama,C., Nakashima,K. and Ishidate,K.
  TITLE     Molecular cloning of mouse choline kinase and choline/ethanolamine
            kinase: their sequence comparison to the respective rat homologs
  JOURNAL   Biochim Biophys Acta 1393 (1), 179-185 (1998)
   PUBMED   9714798
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC133523.3.
            
            On Jul 27, 2022 this sequence version replaced XP_006531709.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.3908800.1,
                                           SRR13422597.9545.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 3.57 cM"
     Protein         1..463
                     /product="choline kinase alpha isoform 4"
                     /EC_number="2.7.1.32"
                     /EC_number="2.7.1.82"
                     /note="ethanolamine kinase; choline/ethanolamine kinase
                     alpha"
                     /calculated_mol_wt=53053
     Region          107..458
                     /region_name="ChoK_euk"
                     /note="Euykaryotic Choline Kinase; cd05156"
                     /db_xref="CDD:270705"
     Site            order(113,115,117..118,120,140,142,200,212..215,217..219,
                     312,314,316..317,319,335..336,339,355,360,426,429)
                     /site_type="active"
                     /db_xref="CDD:270705"
     Site            order(113,118,120,140,142,200,212..215,217..219,316,319,
                     335..336)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270705"
     Site            order(115,117,312,314,317,339,355,360,426,429)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:270705"
     Site            order(184,187..188,191..192,195..196,202..205,247..248,
                     250..251)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270705"
     CDS             1..463
                     /gene="Chka"
                     /gene_synonym="Chetk-alpha; Chk; ChoK; CK; CK/EK-alpha;
                     EK; EtnK-alpha"
                     /coded_by="NM_001410133.1:245..1636"
                     /note="isoform 4 is encoded by transcript variant 5"
                     /db_xref="GeneID:12660"
                     /db_xref="MGI:MGI:107760"
ORIGIN      
        1 mktkfctgge aepsplglll scggnaaptp gvgqqrdaag eleskqlggr tqplalpppp
       61 ppplplpppp sppladeqpe prtrrraylw ckeflpgawr glredqfhis virgglsnml
      121 fqcslpdsia svgdeprkvl lrlygailkm vfliklwngk rscnkegseq aqnenefqga
      181 eamvlesvmf ailaerslgp klfgifpqgr leqfipsrrl dteelrlpdi saeiaekmat
      241 fhgmkmpfnk epkwlfgtme kylnqvlrlk fsrearvqql hkilsynlpl elenlrsllq
      301 ytrspvvfch ndcqegnill legqenserr klmlidfeys synyrgfdig nhfcewmydy
      361 tyekypffra niqkypsrkq qlhfissylt tfqndfesls seeqfatked mllevnrfal
      421 ashflwglws ivqakissie fgymeyaqar feayfdqkrk lgv
//
