LOCUS       NM_028850               2550 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus cysteine-rich hydrophobic domain 2 (Chic2), mRNA.
ACCESSION   NM_028850
VERSION     NM_028850.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643. PMID: 29144450]
REFERENCE   3  (bases 1 to 2550)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   4  (bases 1 to 2550)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   5  (bases 1 to 2550)
  AUTHORS   Cools,J., Mentens,N. and Marynen,P.
  TITLE     A new family of small, palmitoylated, membrane-associated proteins,
            characterized by the presence of a cysteine-rich hydrophobic motif
  JOURNAL   FEBS Lett 492 (3), 204-209 (2001)
   PUBMED   11257495
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161192.12, AK006962.1, AK015681.1 and CF744991.1.
            
            On May 9, 2015 this sequence version replaced NM_028850.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BB609895.1, AK172289.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AC161192.12        170756-170781       c
            27-231              AK006962.1         12-216
            232-232             AC161192.12        170550-170550       c
            233-1112            AK006962.1         218-1097
            1113-2122           AK015681.1         366-1375
            2123-2550           CF744991.1         211-638
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 39.54 cM"
     gene            1..2550
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /note="cysteine-rich hydrophobic domain 2"
                     /db_xref="GeneID:74277"
                     /db_xref="MGI:MGI:1921527"
     exon            1..462
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /inference="alignment:Splign:2.1.0"
     exon            463..517
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /inference="alignment:Splign:2.1.0"
     exon            518..673
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /inference="alignment:Splign:2.1.0"
     exon            674..730
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /inference="alignment:Splign:2.1.0"
     exon            731..790
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /inference="alignment:Splign:2.1.0"
     exon            791..2550
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1045
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
     regulatory      1111..1116
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /note="hexamer: AATACA"
     polyA_site      1135
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /note="major polyA site"
     regulatory      2104..2109
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
                     /note="hexamer: ATTAAA"
     polyA_site      2124
                     /gene="Chic2"
                     /gene_synonym="1700081B18Rik; 4930502K01Rik; BTL"
ORIGIN      
        1 gggaagtctc gcgatgcggt agccggctgc tcctgctgcg ggtggctcgg gctgttgctg
       61 tggttgccgg agttgcggcg gcgacggcgg cggcgctggc gcgggcggcg gcgctggcgg
      121 gggtggcggc cccggcggat gttgacattg tgttgttgtt cctgcccacg gtaatggcgg
      181 cggtggcggc ggcaacggcg gcggccggga ccaggtctcc tcagtagccg gagcccacac
      241 tgccgagcgc cagctccgcg gcctccgcgc ccgccgcctc accagccgtt ccccgcggcc
      301 gccccagagc cagggccccg ggcggggggc tcgccggctc gggatggcgg atttcgatga
      361 aatctacgag gaggaggagg acgaggagcg ggccctggag gagcagctgc tcaagtactc
      421 gcccgacccg gtggtggtcc gcggctccgg tcacgtcacc gtatttggac tgagcaacaa
      481 atttgaatca gaattccctt cttcattaac tggaaaagta gctcctgaag aatttaaagc
      541 cagcatcaac agagttaaca gctgtctcag gaagaatctt cctgttaatg tgcggtggtt
      601 gctttgtggc tgcctgtgct gctgctgcac gttagggtgc agtatgtggc cagttatttg
      661 cctcagtaaa agaacacgaa gatcgattga gaagttatta gaatgggaaa acaataggtt
      721 ataccacaag ctgtgcttgc actggagact gagcaaaagg aaatgtgaaa cgaacaacat
      781 gatggaatat gtcatcctca tagaattttt accaaagaca ccgatttttc gaccagatta
      841 gcatttgctt tatttataga gactttccaa gtgtgttgtc tttccaatgg tgccttgctt
      901 ggtgctctcc tgctggtgac atgatgctgg ttctacagaa ttgggtggtg ttcttgtttg
      961 ttttgttttt taaataaccg catgttctat gtgtgcatat ttgtcaaact ttgcaagtta
     1021 tttcatacag atgtttaata cttaagttat tgtgctcttt tctgttatat attctgattt
     1081 tcaagggtta ctttttgtat tctcaaaaaa aatacattcg aacttagcat aaaaatgggc
     1141 agcctttgtt attttgtcaa caaggttcac acacacagtc ctttacttgt tggtccctta
     1201 ccctaggaaa catagtatct gttacaacat gctcatgcac tgttgtgggc ttccctttag
     1261 aattggccac aacagttgtt tggaaactgt aaattttctc acatggtctt ttgggccctg
     1321 agctgacctt cccaggaacc tcacagttcc ggatcctttg tctcagtaga tacagcttag
     1381 cctcatgaag aactagcagc cgttttgaaa atacctgtct tcaaaagtgg taatttcttt
     1441 agcgtagaca catcacaaat ggtgtgcgtg ttcctacggt tactagagtt caggctcagc
     1501 atagcgtggc ttcaggctcc tgagttccag tggacagggc agtgaggaag ctgtgctcca
     1561 gctagtgtct acttacctac agaagtagag cattgttctt aaagttaaag gtaaaacgtg
     1621 cccgatctaa cataattgta gaatggatgt gtttctgaat agtttggaaa gaaaaagttc
     1681 tagaattaaa aatcatctga tatttgatac cccactggta tatagttatg tagagcaggt
     1741 agttactaag aaatagaggg gacatttaat actattttca gtcttcccat tcactttcat
     1801 ctagttacat acttaatttt attgaaaatt ggatatttaa gttcagatca ggttagctta
     1861 ttgcttttaa atatacacat acatgtattt ttttctgctt ggttgtacat atctccacac
     1921 tatagatttc tgaatgacct atgtgtagac tttgggtttt ataagcctaa aattatacac
     1981 attttaatgt gtgtcaagaa cttgttccat accattgtgg tctagagcaa taatatgaat
     2041 aacgtttgaa attatataaa ctatgcttaa taatttgtaa aaagaattga tacttcccct
     2101 tgtattaaac cttttaaata ccaattttgt taatttatat acctatattt ttaaaaagtg
     2161 ctgctttgta cttctctgaa gtactgtgca tatacacaca cccaaacaca cacacacttt
     2221 aatagctaaa tgccaggatc tcatatccta tatgggactt taggaattgt ctttaaaact
     2281 ttgacttaag gaagtccccg tctgtgccca cccccagctc cttcccccac cacaggaact
     2341 taacagcttg aatccataga aaacttttct gtgaagcact aagtgtgcaa aggattttaa
     2401 tggtttcaca tctttattta atgtttgtct atccagggta ggtgtgtcct accaccataa
     2461 ttagtatagt tcaaagtaat gcggttccaa aatgcagtgt ttcagtgtga agtgattgta
     2521 tattgacaca gagctgaatt agcctcaggc
//
