LOCUS       NM_001289580            3347 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus checkpoint with forkhead and ring finger domains
            (Chfr), transcript variant 5, mRNA.
ACCESSION   NM_001289580
VERSION     NM_001289580.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Tiruppathi,C., Wang,D.M., Ansari,M.O., Bano,S., Tsukasaki,Y.,
            Mukhopadhyay,A., Joshi,J.C., Loch,C., Niessen,H.W.M. and Malik,A.B.
  TITLE     Ubiquitin ligase CHFR mediated degradation of VE-cadherin through
            ubiquitylation disrupts endothelial adherens junctions
  JOURNAL   Nat Commun 14 (1), 6582 (2023)
   PUBMED   37852964
  REMARK    GeneRIF: Ubiquitin ligase CHFR mediated degradation of VE-cadherin
            through ubiquitylation disrupts endothelial adherens junctions.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Lu,L.Y. and Yu,X.
  TITLE     CHFR is important for the survival of male premeiotic germ cells
  JOURNAL   Cell Cycle 14 (21), 3454-3460 (2015)
   PUBMED   26566864
  REMARK    GeneRIF: CHFR is important for the survival of male premeiotic germ
            cells, which is likely through maintaining genomic stability in
            spermatogonial stem cells.
REFERENCE   3  (bases 1 to 3347)
  AUTHORS   Liu,C., Wu,J., Paudyal,S.C., You,Z. and Yu,X.
  TITLE     CHFR is important for the first wave of ubiquitination at DNA
            damage sites
  JOURNAL   Nucleic Acids Res 41 (3), 1698-1710 (2013)
   PUBMED   23268447
REFERENCE   4  (bases 1 to 3347)
  AUTHORS   Kashima,L., Idogawa,M., Mita,H., Shitashige,M., Yamada,T., Ogi,K.,
            Suzuki,H., Toyota,M., Ariga,H., Sasaki,Y. and Tokino,T.
  TITLE     CHFR protein regulates mitotic checkpoint by targeting PARP-1
            protein for ubiquitination and degradation
  JOURNAL   J Biol Chem 287 (16), 12975-12984 (2012)
   PUBMED   22337872
  REMARK    GeneRIF: The interaction between CHFR and PARP-1 plays an important
            role in cell cycle regulation and cancer therapeutic strategies.
REFERENCE   5  (bases 1 to 3347)
  AUTHORS   Jung,I., Sohn,D.H., Choi,J., Kim,J.M., Jeon,S., Seol,J.H. and
            Seong,R.H.
  TITLE     SRG3/mBAF155 stabilizes the SWI/SNF-like BAF complex by blocking
            CHFR mediated ubiquitination and degradation of its major
            components
  JOURNAL   Biochem Biophys Res Commun 418 (3), 512-517 (2012)
   PUBMED   22285184
  REMARK    GeneRIF: CHFR interacts with BRG1, SNF5, and BAF60a of the
            SWI/SNF-like BAF complex and ubiquitinates them to target for
            degradation through a proteasome-mediated pathway; SRG3 stabilizes
            these components by blocking their interaction with CHFR.
REFERENCE   6  (bases 1 to 3347)
  AUTHORS   Wu,J., Chen,Y., Lu,L.Y., Wu,Y., Paulsen,M.T., Ljungman,M.,
            Ferguson,D.O. and Yu,X.
  TITLE     Chfr and RNF8 synergistically regulate ATM activation
  JOURNAL   Nat Struct Mol Biol 18 (7), 761-768 (2011)
   PUBMED   21706008
  REMARK    GeneRIF: RNF8 and Chfr, function together to activate ATM and
            maintain genomic stability in vivo.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3347)
  AUTHORS   Castiel,A., Danieli,M.M., David,A., Moshkovitz,S., Aplan,P.D.,
            Kirsch,I.R., Brandeis,M., Kramer,A. and Izraeli,S.
  TITLE     The Stil protein regulates centrosome integrity and mitosis through
            suppression of Chfr
  JOURNAL   J Cell Sci 124 (Pt 4), 532-539 (2011)
   PUBMED   21245198
  REMARK    GeneRIF: Stil protein regulates centrosome integrity and mitosis
            through suppression of Chfr.
REFERENCE   8  (bases 1 to 3347)
  AUTHORS   Fu,Z., Regan,K., Zhang,L., Muders,M.H., Thibodeau,S.N., French,A.,
            Wu,Y., Kaufmann,S.H., Lingle,W.L., Chen,J. and Tindall,D.J.
  TITLE     Deficiencies in Chfr and Mlh1 synergistically enhance tumor
            susceptibility in mice
  JOURNAL   J Clin Invest 119 (9), 2714-2724 (2009)
   PUBMED   19690386
  REMARK    GeneRIF: Mice with deficiencies in both Chfr and Mlh1 (the gene
            that encodes the DNA mismatch-repair protein Mlh1) displayed
            dramatically higher incidence of spontaneous tumors relative to
            mice deficient for only one of these genes.
REFERENCE   9  (bases 1 to 3347)
  AUTHORS   Yu,X., Minter-Dykhouse,K., Malureanu,L., Zhao,W.M., Zhang,D.,
            Merkle,C.J., Ward,I.M., Saya,H., Fang,G., van Deursen,J. and
            Chen,J.
  TITLE     Chfr is required for tumor suppression and Aurora A regulation
  JOURNAL   Nat Genet 37 (4), 401-406 (2005)
   PUBMED   15793587
  REMARK    GeneRIF: Downregulation of Chfr contributes to tumorigenesis by
            controlling the expression levels of Aurora A.
REFERENCE   10 (bases 1 to 3347)
  AUTHORS   Daniels,M.J., Marson,A. and Venkitaraman,A.R.
  TITLE     PML bodies control the nuclear dynamics and function of the CHFR
            mitotic checkpoint protein
  JOURNAL   Nat Struct Mol Biol 11 (11), 1114-1121 (2004)
   PUBMED   15467728
  REMARK    GeneRIF: PML bodies control the distribution, dynamics and function
            of CHFR
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC118260.7, AK048930.1 and AK169182.1.
            
            Transcript Variant: This variant (5) contains an alternate exon in
            the 5' region, uses an alternate in-frame splice site, and
            initiates translation at a downstream in-frame start codon,
            compared to variant 1. The encoded isoform (5) is shorter than
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK048930.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                AC118260.7         6987-7007
            22-1059             AK048930.1         2-1039
            1060-1236           AK169182.1         916-1092
            1237-2894           AK048930.1         1218-2875
            2895-3347           AC118260.7         42678-43130
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.32 cM"
     gene            1..3347
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /note="checkpoint with forkhead and ring finger domains"
                     /db_xref="GeneID:231600"
                     /db_xref="MGI:MGI:2444898"
     exon            1..105
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            106..249
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            250..419
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            420..519
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            520..629
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            630..689
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            690..905
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     CDS             707..2278
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /EC_number="2.3.2.27"
                     /note="isoform 5 is encoded by transcript variant 5; E3
                     ubiquitin-protein ligase CHFR; RING-type E3 ubiquitin
                     transferase CHFR"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase CHFR isoform 5"
                     /protein_id="NP_001276509.1"
                     /db_xref="GeneID:231600"
                     /db_xref="MGI:MGI:2444898"
                     /translation="MFHVTKDCSGPGQGDDPQVPLLSPMAQTCLEEPQPSTSTSDLLP
                     TASTSSTEPELTSAGQKHSSSSGPGNTSISPKGRSSLVANGELSSLSPVFQDKEASFS
                     LLESKDHEELEPAKKKMKGDGELDTNLQLLVSGQRGNAQTSSEDVKDASVKPDKMEET
                     LTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLV
                     EAYLIQHPDKSRSEEDVRSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSE
                     SSDISQPYIVCRQCPEYRRQAVQSLPCPVPESELGATLALGGEAPSTSASLPTAPDYM
                     CPLQGSHAICTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCFGCL
                     APFCELNLGDKCLDGVLNNNNYESDILKNYLATRGLTWKSVLTESLLALQRGVFMLSD
                     YRITGNTVLCYCCGLRSFRELTYQYRQNIPASELPVTVTSRPDCYWGRNCRTQVKAHH
                     AMKFNHICEQTRFKN"
     exon            906..1070
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1230
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1385
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1548
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1694
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1695..1811
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1895
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1896..1966
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1967..2054
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2162
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2235
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..3347
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3320..3325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /note="hexamer: AATAAA"
     polyA_site      3344
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /note="major polyA site"
ORIGIN      
        1 cccctccccc tcgcctcccg tggatcagct ggggtacccg agatgtctgt tgacagcggt
       61 ggcggcgact gtaaggccgg ttccgggatc cgcgcagggc gtgggatgtg agtcctatgg
      121 agctacacgg ggaagagcag ccgccgccgc cgcaggaacc ctgggggagg cttcttcgtc
      181 taggcgcaga ggaggacgaa ccccagatcc ttctttggaa acgcgagtgg accatcggaa
      241 ggaggagagt tgctcttctg gatgcttgat gatggatatc agtcagttct cagtacaact
      301 ttgtgaaata gatctgtaaa tatatccttc gaaatacgat cttgtcctca ttactgtaca
      361 cttctctttc tcaatgaaat gcaattagaa attattaaca aaaggaaaac tgaaaaaagg
      421 ctgtgacctc tctttcccca gcaataaact ggtctctgga gatcactgta aacttacagt
      481 ggatgaaata tctggtgagg tgacactgga agacaccagc accaatggaa cagtgatcaa
      541 taagctgcaa gttgttaaga agcagactta ccctttacag agcggggata tcatctattt
      601 ggtgtacagg aagaatgagc cagaacacaa tgtggcatac ctctatgaat ctttaagtgg
      661 caaacagagc ttaactcaag actcccttga agccaataag gaaaatatgt tccatgtgac
      721 caaagattgc tcaggtccag ggcagggtga tgatccccag gttccactat tgtcacccat
      781 ggctcagaca tgcttagagg aaccacagcc atcaacatcg acatcagacc tcctccccac
      841 ggcctctacc tcttctacgg agccagagct gacctctgca gggcaaaagc attcttctag
      901 ctctggacct gggaacacaa gcatctcccc aaaaggacgc agttcacttg ttgcaaatgg
      961 cgaactctct agcctttctc cagttttcca agacaaagaa gcatcctttt ctttgctgga
     1021 aagtaaagac catgaggaat tggagcctgc caaaaaaaag atgaaaggag atggggaact
     1081 tgacacgaac ctccagttat tagtttcagg ccagcgtgga aatgcccaaa cctcaagtga
     1141 agatgtcaaa gatgcctctg tgaagccaga caagatggag gagacactaa cctgtatcat
     1201 ctgccaggac cttctgcacg attgtgtgag tttgcagcct tgtatgcaca cattttgtgc
     1261 ggcttgctac tctggttgga tggagcgttc atctctgtgc cctacctgcc gatgtccagt
     1321 ggagcggatt tgcaaaaacc acatcctgaa caacctagtg gaagcatacc ttatccagca
     1381 cccagataaa agtcgcagtg aagaagatgt gagaagtatg gatgcaagga ataaaatcac
     1441 tcaagatatg ctgcaaccca aagtcaggag gtctttctct gatgaagagg ggagttcaga
     1501 ggacctgcta gagctgtctg atgtcgacag tgaatcctca gatatcagtc agccatacat
     1561 tgtctgcaga cagtgtcctg aatacagaag gcaagcggtg cagtctcttc cttgcccagt
     1621 cccagagagt gagctgggag ctacactggc ccttggtggg gaggcacctt caacatctgc
     1681 cagcttgcca acagccccgg attacatgtg ccctcttcaa ggaagccatg ccatatgcac
     1741 ctgctgcttc cagcctatgc ctgaccggag agctgaacgg gagcaggatc cccgcgtcgc
     1801 ccctcagcag tgtgcggtgt gcctgcagcc cttctgccac ctgtactggg gctgcacgag
     1861 gactggctgc tttggctgct tggccccatt ctgtgagctc aacctggggg acaagtgctt
     1921 ggatggagtg ctgaacaata acaattatga atcggacatc ctgaagaatt acctggcaac
     1981 caggggtctg acatggaaaa gtgtgttgac agagagtctc ctggctctgc agcgaggtgt
     2041 atttatgctg tctgattaca gaatcactgg aaatactgtg ctgtgttact gctgtggtct
     2101 gcgtagcttc cgagagctga cctaccagta tcgtcagaac attcctgctt ctgagttgcc
     2161 agtgactgta acatcccgtc ctgactgcta ctggggccgt aactgtcgca ctcaggtgaa
     2221 ggctcaccat gcaatgaaat tcaatcacat ctgtgagcaa acaaggttca agaactgaat
     2281 attggaagag acacgtgaaa cagttacaga gcacaggagg caatgttttt taaaaggccc
     2341 catacaaaca cttgaagggc atttttacag ttttccctac gcagcactgg gggttctggt
     2401 caggcactgg ggtgtggatt ttccctttgt ggagcgtcat cgtcagtgac acacacacca
     2461 cgtgccccag taacctggga ggcctcctgg ccacaccagc cttctagtcg gtgtaagagg
     2521 atcagtagac tgagttctcc ctccctccca ctggaccgct ctccctcctg tgtgctgagg
     2581 cagtgacagt gcaggtttgt acttttgtat cttttctacc ttgtaccttg actatggtta
     2641 aaatgggaag ctcataggaa aagttgtcag ctgtgtcaga cgcattctcc ggaaggaaag
     2701 gaagcatacg tttacagcct ccaggggtac ataggatagt ctcctgtctg gaaagacaca
     2761 gccttttgta tattttttat tttaataagt ttggtgctta tcttctaata agatttaaat
     2821 attaaaaact gtagcacaaa tactataatt tataatttac aaattgacta aaattaggaa
     2881 tagcctcata tttggaagaa tgagcatatg tttctgttta tttaaaaaaa attatcaact
     2941 gtccgaaact gctaatcctg ggaaggaagt cttgagtgtc caggtcctcg acccagggct
     3001 ggctgagccc ttagccctcc agtccctgag agcaactccc tgctgaaact ccagatggct
     3061 ccagactgac agggcctttg ctttccaagc cctcatctgg tacattaggt ttgccatgtc
     3121 atctggaata atacttgaaa ttttgttttt aggtttttta aaactttttt atcttttatt
     3181 gaaatcacct gttatatttg tttgaaaact ggtaactttt tgcttctcag gaaaaaaatt
     3241 ttcaactgtt gtcttgctct tgatacagac tgagaggcag tactctgtgt ttgtgggaga
     3301 tggttctttt tttgattcta ataaatgagc tgtgtttgtt tctcttc
//
