LOCUS       NM_001289577            3180 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus checkpoint with forkhead and ring finger domains
            (Chfr), transcript variant 1, mRNA.
ACCESSION   NM_001289577 XM_006534914
VERSION     NM_001289577.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Tiruppathi,C., Wang,D.M., Ansari,M.O., Bano,S., Tsukasaki,Y.,
            Mukhopadhyay,A., Joshi,J.C., Loch,C., Niessen,H.W.M. and Malik,A.B.
  TITLE     Ubiquitin ligase CHFR mediated degradation of VE-cadherin through
            ubiquitylation disrupts endothelial adherens junctions
  JOURNAL   Nat Commun 14 (1), 6582 (2023)
   PUBMED   37852964
  REMARK    GeneRIF: Ubiquitin ligase CHFR mediated degradation of VE-cadherin
            through ubiquitylation disrupts endothelial adherens junctions.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Lu,L.Y. and Yu,X.
  TITLE     CHFR is important for the survival of male premeiotic germ cells
  JOURNAL   Cell Cycle 14 (21), 3454-3460 (2015)
   PUBMED   26566864
  REMARK    GeneRIF: CHFR is important for the survival of male premeiotic germ
            cells, which is likely through maintaining genomic stability in
            spermatogonial stem cells.
REFERENCE   3  (bases 1 to 3180)
  AUTHORS   Liu,C., Wu,J., Paudyal,S.C., You,Z. and Yu,X.
  TITLE     CHFR is important for the first wave of ubiquitination at DNA
            damage sites
  JOURNAL   Nucleic Acids Res 41 (3), 1698-1710 (2013)
   PUBMED   23268447
REFERENCE   4  (bases 1 to 3180)
  AUTHORS   Kashima,L., Idogawa,M., Mita,H., Shitashige,M., Yamada,T., Ogi,K.,
            Suzuki,H., Toyota,M., Ariga,H., Sasaki,Y. and Tokino,T.
  TITLE     CHFR protein regulates mitotic checkpoint by targeting PARP-1
            protein for ubiquitination and degradation
  JOURNAL   J Biol Chem 287 (16), 12975-12984 (2012)
   PUBMED   22337872
  REMARK    GeneRIF: The interaction between CHFR and PARP-1 plays an important
            role in cell cycle regulation and cancer therapeutic strategies.
REFERENCE   5  (bases 1 to 3180)
  AUTHORS   Jung,I., Sohn,D.H., Choi,J., Kim,J.M., Jeon,S., Seol,J.H. and
            Seong,R.H.
  TITLE     SRG3/mBAF155 stabilizes the SWI/SNF-like BAF complex by blocking
            CHFR mediated ubiquitination and degradation of its major
            components
  JOURNAL   Biochem Biophys Res Commun 418 (3), 512-517 (2012)
   PUBMED   22285184
  REMARK    GeneRIF: CHFR interacts with BRG1, SNF5, and BAF60a of the
            SWI/SNF-like BAF complex and ubiquitinates them to target for
            degradation through a proteasome-mediated pathway; SRG3 stabilizes
            these components by blocking their interaction with CHFR.
REFERENCE   6  (bases 1 to 3180)
  AUTHORS   Wu,J., Chen,Y., Lu,L.Y., Wu,Y., Paulsen,M.T., Ljungman,M.,
            Ferguson,D.O. and Yu,X.
  TITLE     Chfr and RNF8 synergistically regulate ATM activation
  JOURNAL   Nat Struct Mol Biol 18 (7), 761-768 (2011)
   PUBMED   21706008
  REMARK    GeneRIF: RNF8 and Chfr, function together to activate ATM and
            maintain genomic stability in vivo.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 3180)
  AUTHORS   Castiel,A., Danieli,M.M., David,A., Moshkovitz,S., Aplan,P.D.,
            Kirsch,I.R., Brandeis,M., Kramer,A. and Izraeli,S.
  TITLE     The Stil protein regulates centrosome integrity and mitosis through
            suppression of Chfr
  JOURNAL   J Cell Sci 124 (Pt 4), 532-539 (2011)
   PUBMED   21245198
  REMARK    GeneRIF: Stil protein regulates centrosome integrity and mitosis
            through suppression of Chfr.
REFERENCE   8  (bases 1 to 3180)
  AUTHORS   Fu,Z., Regan,K., Zhang,L., Muders,M.H., Thibodeau,S.N., French,A.,
            Wu,Y., Kaufmann,S.H., Lingle,W.L., Chen,J. and Tindall,D.J.
  TITLE     Deficiencies in Chfr and Mlh1 synergistically enhance tumor
            susceptibility in mice
  JOURNAL   J Clin Invest 119 (9), 2714-2724 (2009)
   PUBMED   19690386
  REMARK    GeneRIF: Mice with deficiencies in both Chfr and Mlh1 (the gene
            that encodes the DNA mismatch-repair protein Mlh1) displayed
            dramatically higher incidence of spontaneous tumors relative to
            mice deficient for only one of these genes.
REFERENCE   9  (bases 1 to 3180)
  AUTHORS   Yu,X., Minter-Dykhouse,K., Malureanu,L., Zhao,W.M., Zhang,D.,
            Merkle,C.J., Ward,I.M., Saya,H., Fang,G., van Deursen,J. and
            Chen,J.
  TITLE     Chfr is required for tumor suppression and Aurora A regulation
  JOURNAL   Nat Genet 37 (4), 401-406 (2005)
   PUBMED   15793587
  REMARK    GeneRIF: Downregulation of Chfr contributes to tumorigenesis by
            controlling the expression levels of Aurora A.
REFERENCE   10 (bases 1 to 3180)
  AUTHORS   Daniels,M.J., Marson,A. and Venkitaraman,A.R.
  TITLE     PML bodies control the nuclear dynamics and function of the CHFR
            mitotic checkpoint protein
  JOURNAL   Nat Struct Mol Biol 11 (11), 1114-1121 (2004)
   PUBMED   15467728
  REMARK    GeneRIF: PML bodies control the distribution, dynamics and function
            of CHFR
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC118260.7.
            
            On Jan 24, 2014 this sequence version replaced XM_006534914.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049792.1, AK154038.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               AC118260.7         6987-7091
            106-249             AC118260.7         7208-7351
            250-349             AC118260.7         11511-11610
            350-459             AC118260.7         14708-14817
            460-519             AC118260.7         15748-15807
            520-735             AC118260.7         15929-16144
            736-900             AC118260.7         17627-17791
            901-1060            AC118260.7         22684-22843
            1061-1215           AC118260.7         23493-23647
            1216-1378           AC118260.7         24275-24437
            1379-1524           AC118260.7         25994-26139
            1525-1644           AC118260.7         29956-30075
            1645-1728           AC118260.7         31315-31398
            1729-1799           AC118260.7         33868-33938
            1800-1887           AC118260.7         35163-35250
            1888-1995           AC118260.7         39281-39388
            1996-2068           AC118260.7         40299-40371
            2069-3180           AC118260.7         42019-43130
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.32 cM"
     gene            1..3180
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /note="checkpoint with forkhead and ring finger domains"
                     /db_xref="GeneID:231600"
                     /db_xref="MGI:MGI:2444898"
     exon            1..105
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            106..249
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            250..349
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            350..459
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            460..519
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            520..735
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            736..900
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1060
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1215
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1378
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1524
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1644
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1728
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1799
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1887
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..1995
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2068
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..3180
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3153..3158
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /note="hexamer: AATAAA"
     polyA_site      3177
                     /gene="Chfr"
                     /gene_synonym="5730484M20Rik; C230082M18; RNF116"
                     /note="major polyA site"
ORIGIN      
        1 cccctccccc tcgcctcccg tggatcagct ggggtacccg agatgtctgt tgacagcggt
       61 ggcggcgact gtaaggccgg ttccgggatc cgcgcagggc gtgggatgtg agtcctatgg
      121 agctacacgg ggaagagcag ccgccgccgc cgcaggaacc ctgggggagg cttcttcgtc
      181 taggcgcaga ggaggacgaa ccccagatcc ttctttggaa acgcgagtgg accatcggaa
      241 ggaggagagg ctgtgacctc tctttcccca gcaataaact ggtctctgga gatcactgta
      301 aacttacagt ggatgaaata tctggtgagg tgacactgga agacaccagc accaatggaa
      361 cagtgatcaa taagctgcaa gttgttaaga agcagactta ccctttacag agcggggata
      421 tcatctattt ggtgtacagg aagaatgagc cagaacacaa tgtggcatac ctctatgaat
      481 ctttaagtgg caaacagagc ttaactcaag actcccttga agccaataag gaaaatatgt
      541 tccatgtgac caaagattgc tcaggtccag ggcagggtga tgatccccag gttccactat
      601 tgtcacccat ggctcagaca tgcttagagg aaccacagcc atcaacatcg acatcagacc
      661 tcctccccac ggcctctacc tcttctacgg agccagagct gacctctgca gggcaaaagc
      721 attcttctag ctctggacct gggaacacaa gcatctcccc aaaaggacgc agttcacttg
      781 ttgcaaatgg cgaactctct agcctttctc cagttttcca agacaaagaa gcatcctttt
      841 ctttgctgga aagtaaagac catgaggaat tggagcctgc caaaaaaaag atgaaaggag
      901 atggggaact tgacacgaac ctccagttat tagtttcagg ccagcgtgga aatgcccaaa
      961 cctcaagtga agatgtcaaa gatgcctctg tgaagccaga caagatggag gagacactaa
     1021 cctgtatcat ctgccaggac cttctgcacg attgtgtgag tttgcagcct tgtatgcaca
     1081 cattttgtgc ggcttgctac tctggttgga tggagcgttc atctctgtgc cctacctgcc
     1141 gatgtccagt ggagcggatt tgcaaaaacc acatcctgaa caacctagtg gaagcatacc
     1201 ttatccagca cccagataaa agtcgcagtg aagaagatgt gagaagtatg gatgcaagga
     1261 ataaaatcac tcaagatatg ctgcaaccca aagtcaggag gtctttctct gatgaagagg
     1321 ggagttcaga ggacctgcta gagctgtctg atgtcgacag tgaatcctca gatatcagtc
     1381 agccatacat tgtctgcaga cagtgtcctg aatacagaag gcaagcggtg cagtctcttc
     1441 cttgcccagt cccagagagt gagctgggag ctacactggc ccttggtggg gaggcacctt
     1501 caacatctgc cagcttgcca acagcagccc cggattacat gtgccctctt caaggaagcc
     1561 atgccatatg cacctgctgc ttccagccta tgcctgaccg gagagctgaa cgggagcagg
     1621 atccccgcgt cgcccctcag cagtgtgcgg tgtgcctgca gcccttctgc cacctgtact
     1681 ggggctgcac gaggactggc tgctttggct gcttggcccc attctgtgag ctcaacctgg
     1741 gggacaagtg cttggatgga gtgctgaaca ataacaatta tgaatcggac atcctgaaga
     1801 attacctggc aaccaggggt ctgacatgga aaagtgtgtt gacagagagt ctcctggctc
     1861 tgcagcgagg tgtatttatg ctgtctgatt acagaatcac tggaaatact gtgctgtgtt
     1921 actgctgtgg tctgcgtagc ttccgagagc tgacctacca gtatcgtcag aacattcctg
     1981 cttctgagtt gccagtgact gtaacatccc gtcctgactg ctactggggc cgtaactgtc
     2041 gcactcaggt gaaggctcac catgcaatga aattcaatca catctgtgag caaacaaggt
     2101 tcaagaactg aatattggaa gagacacgtg aaacagttac agagcacagg aggcaatgtt
     2161 ttttaaaagg ccccatacaa acacttgaag ggcattttta cagttttccc tacgcagcac
     2221 tgggggttct ggtcaggcac tggggtgtgg attttccctt tgtggagcgt catcgtcagt
     2281 gacacacaca ccacgtgccc cagtaacctg ggaggcctcc tggccacacc agccttctag
     2341 tcggtgtaag aggatcagta gactgagttc tccctccctc ccactggacc gctctccctc
     2401 ctgtgtgctg aggcagtgac agtgcaggtt tgtacttttg tatcttttct accttgtacc
     2461 ttgactatgg ttaaaatggg aagctcatag gaaaagttgt cagctgtgtc agacgcattc
     2521 tccggaagga aaggaagcat acgtttacag cctccagggg tacataggat agtctcctgt
     2581 ctggaaagac acagcctttt gtatattttt tattttaata agtttggtgc ttatcttcta
     2641 ataagattta aatattaaaa actgtagcac aaatactata atttataatt tacaaattga
     2701 ctaaaattag gaatagcctc atatttggaa gaatgagcat atgtttctgt ttatttaaaa
     2761 aaaattatca actgtccgaa actgctaatc ctgggaagga agtcttgagt gtccaggtcc
     2821 tcgacccagg gctggctgag cccttagccc tccagtccct gagagcaact ccctgctgaa
     2881 actccagatg gctccagact gacagggcct ttgctttcca agccctcatc tggtacatta
     2941 ggtttgccat gtcatctgga ataatacttg aaattttgtt tttaggtttt ttaaaacttt
     3001 tttatctttt attgaaatca cctgttatat ttgtttgaaa actggtaact ttttgcttct
     3061 caggaaaaaa attttcaact gttgtcttgc tcttgataca gactgagagg cagtactctg
     3121 tgtttgtggg agatggttct ttttttgatt ctaataaatg agctgtgttt gtttctcttc
//
