LOCUS       NM_016681               2301 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus checkpoint kinase 2 (Chek2), transcript variant 1,
            mRNA.
ACCESSION   NM_016681
VERSION     NM_016681.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Lin,X., Gupta,D., Vaitsiankova,A., Bhandari,S.K., Leung,K.S.K.,
            Menolfi,D., Lee,B.J., Russell,H.R., Gershik,S., Huang,X., Gu,W.,
            McKinnon,P.J., Dantzer,F., Rothenberg,E., Tomkinson,A.E. and Zha,S.
  TITLE     Inactive Parp2 causes Tp53-dependent lethal anemia by blocking
            replication-associated nick ligation in erythroblasts
  JOURNAL   Mol Cell 84 (20), 3916-3931 (2024)
   PUBMED   39383878
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Bai,L., Li,P., Xiang,Y., Jiao,X., Chen,J., Song,L., Liang,Z.,
            Liu,Y., Zhu,Y. and Lu,L.Y.
  TITLE     BRCA1 safeguards genome integrity by activating chromosome
            asynapsis checkpoint to eliminate recombination-defective oocytes
  JOURNAL   Proc Natl Acad Sci U S A 121 (19), e2401386121 (2024)
   PUBMED   38696471
REFERENCE   3  (bases 1 to 2301)
  AUTHORS   Xu,P., Gao,Y., Jiang,S., Cui,Y., Xie,Y., Kang,Z., Chen,Y.X., Sun,D.
            and Fang,J.Y.
  TITLE     CHEK2 deficiency increase the response to PD-1 inhibitors by
            affecting the tumor immune microenvironment
  JOURNAL   Cancer Lett 588, 216595 (2024)
   PUBMED   38097135
  REMARK    GeneRIF: CHEK2 deficiency increase the response to PD-1 inhibitors
            by affecting the tumor immune microenvironment.
REFERENCE   4  (bases 1 to 2301)
  AUTHORS   Lu,J., Sun,W., Liu,B., Zhang,J., Wang,R., Goltzman,D. and Miao,D.
  TITLE     Chk2 Modulates Bmi1-Deficiency-Induced Renal Aging and Fibrosis via
            Oxidative Stress, DNA Damage, and p53/TGFbeta1-Induced
            Epithelial-Mesenchymal Transition
  JOURNAL   Int J Biol Sci 20 (6), 2008-2026 (2024)
   PUBMED   38617548
  REMARK    GeneRIF: Chk2 Modulates Bmi1-Deficiency-Induced Renal Aging and
            Fibrosis via Oxidative Stress, DNA Damage, and p53/TGFbeta1-Induced
            Epithelial-Mesenchymal Transition.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2301)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 2301)
  AUTHORS   Takai,H., Naka,K., Okada,Y., Watanabe,M., Harada,N., Saito,S.,
            Anderson,C.W., Appella,E., Nakanishi,M., Suzuki,H., Nagashima,K.,
            Sawa,H., Ikeda,K. and Motoyama,N.
  TITLE     Chk2-deficient mice exhibit radioresistance and defective
            p53-mediated transcription
  JOURNAL   EMBO J 21 (19), 5195-5205 (2002)
   PUBMED   12356735
  REMARK    GeneRIF: results show that Chk2 plays a critical role in p53
            function in response to IR by regulating its transcriptional
            activity as well as its stability
REFERENCE   7  (bases 1 to 2301)
  AUTHORS   Hirao,A., Cheung,A., Duncan,G., Girard,P.M., Elia,A.J., Wakeham,A.,
            Okada,H., Sarkissian,T., Wong,J.A., Sakai,T., De Stanchina,E.,
            Bristow,R.G., Suda,T., Lowe,S.W., Jeggo,P.A., Elledge,S.J. and
            Mak,T.W.
  TITLE     Chk2 is a tumor suppressor that regulates apoptosis in both an
            ataxia telangiectasia mutated (ATM)-dependent and an
            ATM-independent manner
  JOURNAL   Mol Cell Biol 22 (18), 6521-6532 (2002)
   PUBMED   12192050
  REMARK    GeneRIF: Chk2 regulates apoptosis in both an ataxia telangiectasia
            mutated (ATM)-dependent and an ATM-independent manner
REFERENCE   8  (bases 1 to 2301)
  AUTHORS   Jack,M.T., Woo,R.A., Hirao,A., Cheung,A., Mak,T.W. and Lee,P.W.
  TITLE     Chk2 is dispensable for p53-mediated G1 arrest but is required for
            a latent p53-mediated apoptotic response
  JOURNAL   Proc Natl Acad Sci U S A 99 (15), 9825-9829 (2002)
   PUBMED   12097646
  REMARK    GeneRIF: role of Chk2 in p53-dependent apoptotic response
REFERENCE   9  (bases 1 to 2301)
  AUTHORS   Hirao,A., Kong,Y.Y., Matsuoka,S., Wakeham,A., Ruland,J.,
            Yoshida,H., Liu,D., Elledge,S.J. and Mak,T.W.
  TITLE     DNA damage-induced activation of p53 by the checkpoint kinase Chk2
  JOURNAL   Science 287 (5459), 1824-1827 (2000)
   PUBMED   10710310
REFERENCE   10 (bases 1 to 2301)
  AUTHORS   Matsuoka,S., Huang,M. and Elledge,S.J.
  TITLE     Linkage of ATM to cell cycle regulation by the Chk2 protein kinase
  JOURNAL   Science 282 (5395), 1893-1897 (1998)
   PUBMED   9836640
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ079787.1, AK044913.1 and BX512104.1.
            
            On May 17, 2018 this sequence version replaced NM_016681.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.1328022.1,
                                           SRR5314788.99447.3 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-34                CJ079787.1         2-35
            35-2281             AK044913.1         2-2248
            2282-2301           BX512104.1         332-351
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 53.73 cM"
     gene            1..2301
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /note="checkpoint kinase 2"
                     /db_xref="GeneID:50883"
                     /db_xref="MGI:MGI:1355321"
     exon            1..66
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            67..404
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..1714
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serine/threonine-protein kinase Chk2; Cds1 homolog; Rad53
                     homolog; CHK2 checkpoint homolog"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Chk2 isoform 1"
                     /protein_id="NP_057890.1"
                     /db_xref="CCDS:CCDS19533.1"
                     /db_xref="GeneID:50883"
                     /db_xref="MGI:MGI:1355321"
                     /translation="MKSHHQSHSSTSSKAHDSASCSQSQGGFSQPQGTPSQLHELSQY
                     QGSSSSSTGTVPSSSQSSHSSSGTLSSLETVSTQELCSIPEDQEPEEPGPAPWARLWA
                     LQDGFSNLDCVNDNYWFGRDKSCEYCFDGPLLRRTDKYRTYSKKHFRIFREMGPKNCY
                     IVYIEDHSGNGTFVNTELIGKGKRCPLSNNSEIALSLCRNKVFVFFDLTVDDQSVYPK
                     ELRDEYIMSKTLGSGACGEVKMAFERKTCQKVAIKIISKRRFALGSSREADTAPSVET
                     EIEILKKLNHPCIIKIKDVFDAEDYYIVLELMEGGELFDRVVGNKRLKEATCKLYFYQ
                     MLVAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGQSKILGETSLMRTLCGTP
                     TYLAPEVLVSNGTAGYSRAVDCWSLGVILFICLSGYPPFSEHKTQVSLKDQITSGKYN
                     FIPEVWTDVSEEALDLVKKLLVVDPKARLTTEEALNHPWLQDEYMKKKFQDLLVQEKN
                     SVTLPVAPAQTSSQKRPLELEVEGMPSTKRLSVCGAVL"
     exon            405..529
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            530..677
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            678..768
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            769..877
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            878..931
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            932..993
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1093
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1180
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1344
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1460
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1546
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1627
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     exon            1628..2288
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2265..2270
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /note="hexamer: ATTAAA"
     polyA_site      2288
                     /gene="Chek2"
                     /gene_synonym="Cds1; CHK2; HUCDS1; Rad53"
                     /note="major polyA site"
ORIGIN      
        1 gtaggtttag cgccactcca ctggcggagg ctgtgcagtg tttgcggctc ctggtggtct
       61 cacgcggtcg gatatgaaga gtcatcatca gtcccacagc agcacctcct cgaaggctca
      121 tgacagtgct tcctgttcac agtcccaggg tggcttcagc cagccccaag gcactccttc
      181 tcagttgcac gagctctcac agtatcaggg ttcgtccagc tcctctaccg gcacagtgcc
      241 gtcctccagc cagtcctcgc actccagttc tgggactctg agctcgctgg agacagtgtc
      301 tacccaggaa ctctgttcta ttcctgagga ccaagaacct gaagaacctg gtcctgcccc
      361 ttgggctcgg ctatgggctc ttcaggatgg attctccaat ctagactgtg ttaacgacaa
      421 ctactggttt gggagagata aaagctgtga atattgcttc gatggaccac tgttgagaag
      481 gacggacaag taccggactt acagcaagaa gcattttcgt attttcaggg aaatgggccc
      541 taaaaattgt tacatcgtat acatagagga tcacagtgga aatggaacct tcgtaaatac
      601 cgagcttatt gggaaaggca aacgctgtcc tctgagtaac aactctgaaa tcgcactttc
      661 actatgtaga aataaagttt ttgtattttt tgatctgact gtagatgatc agtcagttta
      721 tcctaaggaa ttaagagacg aatacatcat gtcaaaaact cttggaagtg gtgcgtgtgg
      781 ggaggtaaag atggcttttg agaggaagac atgtcagaaa gtggccataa agatcattag
      841 caagcggagg tttgctcttg gctcatcgag agaagccgac acagctccca gtgtggaaac
      901 tgaaatagaa attttgaaga aactaaatca tccatgcatc atcaagatta aagatgtttt
      961 tgatgcggaa gattattaca ttgttctgga actgatggaa ggaggagaac tatttgaccg
     1021 ggtggtgggg aacaagcgcc tgaaagaagc cacctgtaag ctctacttct accagatgct
     1081 tgtagctgta cagtaccttc acgaaaatgg gatcatacat cgggacttaa agccggagaa
     1141 tgttctttta tcatctcagg aagaggattg tctaatcaag atcactgact ttgggcagtc
     1201 caagatcttg ggggagacct ccttgatgag aaccttatgt ggtacgccca cttatctggc
     1261 tcctgaggtt cttgtctcca acgggactgc tgggtacagc cgcgctgtgg actgctggag
     1321 tttaggagtt attcttttca tctgcctaag tgggtatcca cctttctctg agcataagac
     1381 ccaagtgtcc ctgaaggatc agatcaccag tggaaagtac aactttattc ctgaagtctg
     1441 gacagatgtc tcagaggagg ctctggacct tgtcaagaaa ctgttagttg tagacccaaa
     1501 ggctcggctt accacagagg aggccttaaa tcatccgtgg cttcaggatg agtacatgaa
     1561 gaagaaattt caggatctcc tggtgcagga aaagaactcg gtgaccctcc ctgtggctcc
     1621 cgcccagact tccagtcaaa agcggcccct ggaactggag gtggagggta tgccgagcac
     1681 aaaacgcctg tctgtgtgtg gggctgtgtt gtgagctttg aatgtgaaag aaatgtgctt
     1741 ccttccacgc tccaattgtc ttctccatct tgacatcttt tttttatatg tatatatttt
     1801 ttaaaaaata tagccaggca gttgtggccc actcctttaa tcccagcact tgggaggcag
     1861 aggcaggcag atttctgagt tcaaggccag cctggtctac agagtgagtt ccaggatagc
     1921 caggactaca cagagaaacc ctgtcttgga aaaaaaaaca aacaagaaac aaaacaaaat
     1981 agcctatatg atggaacaat tgcttttcca tcacagatac acgattaaaa aacgtggaaa
     2041 tctgggctga ttgtttgatg acctgcactt tagatttctt tctttctttc tttcatttta
     2101 agaaaaatgt tagggggtgg tgttgagaga ttaacaccac tcctgttgag ggctgcagtt
     2161 cagttcccag cacccatgta actacatttc tggatcggat gccctcacct ggcctctctg
     2221 gtcacctact acacatgtgt tcatgtacac agaaacacat aatcattaaa aagaatattc
     2281 taaaacaaaa aaaaaaaaaa a
//
