LOCUS       NM_001410207            3204 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus checkpoint kinase 1 (Chek1), transcript variant 4,
            mRNA.
ACCESSION   NM_001410207
VERSION     NM_001410207.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Lin,X., Gupta,D., Vaitsiankova,A., Bhandari,S.K., Leung,K.S.K.,
            Menolfi,D., Lee,B.J., Russell,H.R., Gershik,S., Huang,X., Gu,W.,
            McKinnon,P.J., Dantzer,F., Rothenberg,E., Tomkinson,A.E. and Zha,S.
  TITLE     Inactive Parp2 causes Tp53-dependent lethal anemia by blocking
            replication-associated nick ligation in erythroblasts
  JOURNAL   Mol Cell 84 (20), 3916-3931 (2024)
   PUBMED   39383878
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Fan,J., Du,X., Chen,M., Xu,Y., Xu,J., Lu,L., Zhou,S., Kong,X.,
            Xu,K. and Zhang,H.
  TITLE     Critical role of checkpoint kinase 1 in spinal cord injury-induced
            motor dysfunction in mice
  JOURNAL   Int Immunopharmacol 138, 112521 (2024)
   PUBMED   38917519
  REMARK    GeneRIF: Critical role of checkpoint kinase 1 in spinal cord
            injury-induced motor dysfunction in mice.
REFERENCE   3  (bases 1 to 3204)
  AUTHORS   Wang,Z., Zhang,L., Yang,J., Zeng,Y., Su,C., Yao,M., Zhang,H.,
            Hu,W., Liu,Y., Lai,Y., Wang,X., Zeng,J. and Liu,R.
  TITLE     Chronic stress induces Alzheimer's disease-like pathologies through
            DNA damage-Chk1-CIP2A signaling
  JOURNAL   Aging (Albany NY) 16 (10), 9168-9187 (2024)
   PUBMED   38819231
  REMARK    GeneRIF: Chronic stress induces Alzheimer's disease-like
            pathologies through DNA damage-Chk1-CIP2A signaling.
REFERENCE   4  (bases 1 to 3204)
  AUTHORS   Shentu,Y., Chen,M., Wang,H., Du,X., Zhang,W., Xie,G., Zhou,S.,
            Ding,L., Zhu,Y., Zhu,M., Zhang,N., Du,C., Ma,J., Chen,R., Yang,J.,
            Fan,X., Gong,Y., Zhang,H. and Fan,J.
  TITLE     Hydrogen sulfide ameliorates lipopolysaccharide-induced
            anxiety-like behavior by inhibiting checkpoint kinase 1 activation
            in the hippocampus of mice
  JOURNAL   Exp Neurol 371, 114586 (2024)
   PUBMED   37898396
REFERENCE   5  (bases 1 to 3204)
  AUTHORS   Chen,J.W., Shan,T.K., Wei,T.W., Jiang,Q.Q., Du,C., Gu,L.F.,
            Yang,T.T., Zhou,L.H., Wang,S.B., Bao,Y.L., Wang,H., Ji,Y.,
            Xie,L.P., Gu,A.H., Sun,C.Q., Wang,Q.M. and Wang,L.S.
  TITLE     SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1
            inhibition combined with gemcitabine treatment induced
            cardiotoxicity in hiPSC-CMs and mice
  JOURNAL   Arch Toxicol 97 (12), 3209-3226 (2023)
   PUBMED   37798514
  REMARK    GeneRIF: SIRT3-dependent mitochondrial redox homeostasis mitigates
            CHK1 inhibition combined with gemcitabine treatment induced
            cardiotoxicity in hiPSC-CMs and mice.
REFERENCE   6  (bases 1 to 3204)
  AUTHORS   Liu,Q., Guntuku,S., Cui,X.S., Matsuoka,S., Cortez,D., Tamai,K.,
            Luo,G., Carattini-Rivera,S., DeMayo,F., Bradley,A., Donehower,L.A.
            and Elledge,S.J.
  TITLE     Chk1 is an essential kinase that is regulated by Atr and required
            for the G(2)/M DNA damage checkpoint
  JOURNAL   Genes Dev 14 (12), 1448-1459 (2000)
   PUBMED   10859164
REFERENCE   7  (bases 1 to 3204)
  AUTHORS   Takai,H., Tominaga,K., Motoyama,N., Minamishima,Y.A., Nagahama,H.,
            Tsukiyama,T., Ikeda,K., Nakayama,K., Nakanishi,M. and Nakayama,K.
  TITLE     Aberrant cell cycle checkpoint function and early embryonic death
            in Chk1(-/-) mice
  JOURNAL   Genes Dev 14 (12), 1439-1447 (2000)
   PUBMED   10859163
REFERENCE   8  (bases 1 to 3204)
  AUTHORS   Chen,P., Luo,C., Deng,Y., Ryan,K., Register,J., Margosiak,S.,
            Tempczyk-Russell,A., Nguyen,B., Myers,P., Lundgren,K., Kan,C.C. and
            O'Connor,P.M.
  TITLE     The 1.7 A crystal structure of human cell cycle checkpoint kinase
            Chk1: implications for Chk1 regulation
  JOURNAL   Cell 100 (6), 681-692 (2000)
   PUBMED   10761933
REFERENCE   9  (bases 1 to 3204)
  AUTHORS   Flaggs,G., Plug,A.W., Dunks,K.M., Mundt,K.E., Ford,J.C.,
            Quiggle,M.R., Taylor,E.M., Westphal,C.H., Ashley,T., Hoekstra,M.F.
            and Carr,A.M.
  TITLE     Atm-dependent interactions of a mammalian chk1 homolog with meiotic
            chromosomes
  JOURNAL   Curr Biol 7 (12), 977-986 (1997)
   PUBMED   9382850
REFERENCE   10 (bases 1 to 3204)
  AUTHORS   Sanchez,Y., Wong,C., Thoma,R.S., Richman,R., Wu,Z.,
            Piwnica-Worms,H. and Elledge,S.J.
  TITLE     Conservation of the Chk1 checkpoint pathway in mammals: linkage of
            DNA damage to Cdk regulation through Cdc25
  JOURNAL   Science 277 (5331), 1497-1501 (1997)
   PUBMED   9278511
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155921.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR17253011.3041673.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164142 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                AC155921.2         93690-93749
            61-142              AC155921.2         94254-94335
            143-366             AC155921.2         96112-96335
            367-431             AC155921.2         97307-97371
            432-501             AC155921.2         97459-97528
            502-690             AC155921.2         100440-100628
            691-795             AC155921.2         101697-101801
            796-891             AC155921.2         103611-103706
            892-1000            AC155921.2         105599-105707
            1001-1178           AC155921.2         106272-106449
            1179-1310           AC155921.2         106680-106811
            1311-1412           AC155921.2         107989-108090
            1413-3204           AC155921.2         109702-111493
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 20.67 cM"
     gene            1..3204
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /note="checkpoint kinase 1"
                     /db_xref="GeneID:12649"
                     /db_xref="MGI:MGI:1202065"
     exon            1..60
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            61..142
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            143..366
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            367..431
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            432..501
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            502..690
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            691..795
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            796..891
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1000
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1178
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1310
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            1311..1412
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..3204
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2216..2221
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /note="hexamer: AATAAA"
     polyA_site      2237
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /note="major polyA site"
     regulatory      2387..2392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /note="hexamer: ATTAAA"
     polyA_site      2406
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /note="major polyA site"
     regulatory      3184..3189
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
                     /note="hexamer: AATACA"
     polyA_site      3204
                     /gene="Chek1"
                     /gene_synonym="Chk1; rad27"
ORIGIN      
        1 cggagcccgg gctggagcgg gtggcccgtg gctctccagc ataaagggac cagagcgatt
       61 tcgctgtgct tggagtcatg gcagtgcctt ttgtggaaga ctgggatttg gtgcaaactt
      121 tgggagaagg tgcctatgga gaagttcaac ttgctgtgaa tagaataact gaagaagctg
      181 ttgcagtgaa aattgtagac atgaagcggg ccatagactg tccagaaaat attaagaaag
      241 agatctgcat caataaaatg ttaagccacg agaatgtagt gaaattctat ggccacagga
      301 gggaaggcca tatccagtat ctgtttctgg agtactgtag tggaggagaa ctttttgata
      361 gaattgagcc agacataggg atgcctgaac aagatgctca gaggttcttc caccaactca
      421 tggcaggggt ggtttatctt catggaattg gaataactca cagggatatt aaaccagaaa
      481 acctcctctt ggatgaaagg gataacctca aaatctctga ctttggcttg gcaacggtat
      541 ttcggcataa taatcgtgaa cgcttactga acaagatgtg tgggacttta ccttatgttg
      601 ctccggagct tctaaagaga aaagaatttc atgcagaacc agttgatgtt tggtcctgtg
      661 gaatagtact tactgcaatg ttggctggag aattgccgtg ggaccagccc agtgatagct
      721 gtcaggaata ttctgattgg aaagaaaaaa aaacctatct caatccttgg aaaaaaattg
      781 attctgctcc tctggctttg cttcataaaa ttctagttga gactccatca gcaaggatca
      841 ccatcccaga cattaagaaa gatagatggt acaacaaacc acttaacaga ggagcaaaga
      901 ggccacgcgc cacatcaggt ggtatgtcag agtcttctag tggattctct aagcacattc
      961 attccaattt ggacttttct ccagtaaata atggttccag tgaagaaacc gtgaagttct
     1021 ctagttccca gccagagccg agaacagggc tttccttgtg ggacactggt ccctcgaacg
     1081 tggacaaact ggttcagggc atcagttttt cccagcctac gtgtcctgag catatgcttg
     1141 taaacagtca gttactcggt acccctggat cttcacagaa cccctggcag cgcttggtca
     1201 aaaggatgac acgattcttt actaaattgg atgcggacaa atcttaccaa tgcctgaaag
     1261 agaccttcga gaagttgggc tatcagtgga agaagagttg tatgaatcag gttactgtat
     1321 caacaactga tagaagaaac aataagttga ttttcaaaat aaatttggta gaaatggatg
     1381 agaagatact ggttgacttc cgactttcta agggtgatgg attagagttc aagagacact
     1441 tcctgaagat taaagggaag ctcagcgatg ttgtgagcag ccagaaggtt tggtttcctg
     1501 ttacatgagg aagctgtcag ctctgcacat tcctggtgaa tagagtgctg ctatgtgaca
     1561 tttttcttcc tagagaagat tatcctattc tgcaaactgc aaacaatagt tgttgaagag
     1621 ttctcttccc attacccaaa catcttccga tttgtagtgt ttggcataca aatactactg
     1681 tattttaatt gtatgtaatg ctttggggaa aggatggatc aaattcatta ggtatttctc
     1741 cagctgtctt taaattgtct ggatttgaaa ccaagttatg ggatacttga gtttgccagc
     1801 ttttataccc atgtagtagt atcacttttg aaaaatcaaa agcttgtttc atcccaagca
     1861 aaatattttc ttctctgcct atttaattgt aaggatgaat aaacacagac catatacagt
     1921 tgattggttc atgaatgagg ccagccacaa aaatgtgtat gttaatgtat gtactgtatt
     1981 ttcagtttgg gtatatgtgc tgcacaaggg cttgaccaat tataaaactt ttttgagtta
     2041 aagtgtttta aattgcaaat tttcctcctg gcgtagcttg tcaaatataa agactaattt
     2101 ggttggattt tcacaaagtt gaacataaac tctagtgctt aagtgacatt actctaaaaa
     2161 ttacacactt aaacaatttt ttctagattc acagccatat tttaattact ctagaaataa
     2221 actattttca aaatcaatat ctaactctga acttattggc cagccaccaa gtttctagat
     2281 gacactgact gaaaaggggc gggagtaagg aaatgcagga gttgaccgga tccttgttcc
     2341 tcaccttgta cactcactta aactttgttt tgttcataaa atttatatta aaatatgaat
     2401 atatgattgg ggtcctgtat tccttttcat agataaattc caacattagt tagtctgaaa
     2461 gctgagaaca tttaaaattt tatggctcaa gtttatttca tgttggtata tgctgttgtc
     2521 acccacttaa ctctcccatc cccaaaagtt aacttgaacc taactctatt cagtctcctg
     2581 tttcccactt gtagtcccac tgtggtaaaa gtcagccatc ttgttaaaaa cgttacttat
     2641 tcgaacccca agtcccatct gcgctgtact gttttatgtc attgtcttct tcatctgatg
     2701 gtatgcagta ctgtcttccg agcctgtatc tctcaagtca tgattgcttt ccgccattac
     2761 ttggcaccta ctcaactata ttgctttggt ctccatgatt ttgaatattc ttgtacttca
     2821 tataagtaga attgtatatt tgcctcttag tgtttgggtt acttcataac aactcttttc
     2881 aggatttatc tatgttataa catatcaggt ttcattcctt ttggtgacta atactccata
     2941 ttatgtatgt ctgtgcgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtatgtatac
     3001 tctttcatat gatcatgcat atttggattg ttgccacctt ttgtccatgt aattgatact
     3061 ggtgtgaaca tgaatatata agtacctgct caagtatgtt tttaattttt tgtgtataga
     3121 ccctgaggta gaattgctat gtcatatgat aattgtgtgt tgtttatttt tttccgctta
     3181 taaaatacaa caatttattt aaaa
//
