LOCUS       NM_201637               8489 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus chromodomain helicase DNA binding protein 8 (Chd8),
            mRNA.
ACCESSION   NM_201637 XM_006519476 XM_619244 XM_907992 XM_987619
VERSION     NM_201637.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Megagiannis,P., Mei,Y., Yan,R.E., Yuan,L., Wilde,J.J.,
            Eckersberg,H., Suresh,R., Tan,X., Chen,H., Farmer,W.T., Cha,K.,
            Le,P.U., Catoire,H., Rochefort,D., Kwan,T., Yee,B.A., Dion,P.,
            Krishnaswamy,A., Cloutier,J.F., Stifani,S., Petrecca,K., Yeo,G.W.,
            Murai,K.K., Feng,G., Rouleau,G.A., Ideker,T., Sanjana,N.E. and
            Zhou,Y.
  TITLE     Autism-associated CHD8 controls reactive gliosis and
            neuroinflammation via remodeling chromatin in astrocytes
  JOURNAL   Cell Rep 43 (8), 114637 (2024)
   PUBMED   39154337
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Cesari,E., Farini,D., Medici,V., Ehrmann,I., Guerra,M., Testa,E.,
            Naro,C., Geloso,M.C., Pagliarini,V., La Barbera,L., D'Amelio,M.,
            Orsini,T., Vecchioli,S.F., Tamagnone,L., Fort,P., Viscomi,M.T.,
            Elliott,D.J. and Sette,C.
  TITLE     Differential expression of paralog RNA binding proteins establishes
            a dynamic splicing program required for normal cerebral cortex
            development
  JOURNAL   Nucleic Acids Res 52 (8), 4167-4184 (2024)
   PUBMED   38324473
REFERENCE   3  (bases 1 to 8489)
  AUTHORS   Ellingford,R.A., Tojo,M., Basson,M.A. and Andreae,L.C.
  TITLE     Male-Dominant Effects of Chd8 Haploinsufficiency on Synaptic
            Phenotypes during Development in Mouse Prefrontal Cortex
  JOURNAL   ACS Chem Neurosci 15 (8), 1635-1642 (2024)
   PUBMED   38557009
  REMARK    GeneRIF: Male-Dominant Effects of Chd8 Haploinsufficiency on
            Synaptic Phenotypes during Development in Mouse Prefrontal Cortex.
REFERENCE   4  (bases 1 to 8489)
  AUTHORS   Tabbaa,M. and Levitt,P.
  TITLE     Chd8 haploinsufficiency impacts rearing experience in C57BL/6 mice
  JOURNAL   Genes Brain Behav 23 (2), e12892 (2024)
   PUBMED   38560770
  REMARK    GeneRIF: Chd8 haploinsufficiency impacts rearing experience in
            C57BL/6 mice.
REFERENCE   5  (bases 1 to 8489)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 8489)
  AUTHORS   Ishihara,K., Oshimura,M. and Nakao,M.
  TITLE     CTCF-dependent chromatin insulator is linked to epigenetic
            remodeling
  JOURNAL   Mol Cell 23 (5), 733-742 (2006)
   PUBMED   16949368
REFERENCE   7  (bases 1 to 8489)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 8489)
  AUTHORS   Nishiyama,M., Nakayama,K., Tsunematsu,R., Tsukiyama,T., Kikuchi,A.
            and Nakayama,K.I.
  TITLE     Early embryonic death in mice lacking the beta-catenin-binding
            protein Duplin
  JOURNAL   Mol Cell Biol 24 (19), 8386-8394 (2004)
   PUBMED   15367660
REFERENCE   9  (bases 1 to 8489)
  AUTHORS   Kobayashi,M., Kishida,S., Fukui,A., Michiue,T., Miyamoto,Y.,
            Okamoto,T., Yoneda,Y., Asashima,M. and Kikuchi,A.
  TITLE     Nuclear localization of Duplin, a beta-catenin-binding protein, is
            essential for its inhibitory activity on the Wnt signaling pathway
  JOURNAL   J Biol Chem 277 (8), 5816-5822 (2002)
   PUBMED   11744694
REFERENCE   10 (bases 1 to 8489)
  AUTHORS   Sakamoto,I., Kishida,S., Fukui,A., Kishida,M., Yamamoto,H.,
            Hino,S., Michiue,T., Takada,S., Asashima,M. and Kikuchi,A.
  TITLE     A novel beta-catenin-binding protein inhibits
            beta-catenin-dependent Tcf activation and axis formation
  JOURNAL   J Biol Chem 275 (42), 32871-32878 (2000)
   PUBMED   10921920
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC126037.4 and AC159323.3.
            
            On or before Jun 20, 2019 this sequence version replaced
            XM_006519476.3, NM_201637.2.
            
            Summary: This gene encodes a member of the
            chromodomain-helicase-DNA binding protein family, which is
            characterized by a SNF2-like domain and two chromatin organization
            modifier domains. The encoded protein also contains brahma and
            kismet domains, which is common to the subfamily of
            chromodomain-helicase-DNA binding proteins to which this protein
            belongs. In mammals, this gene has been shown to function in
            several processes including transcriptional regulation, epigenetic
            remodeling, promotion of cell proliferation, and regulation of RNA
            synthesis. Knockout of this gene causes early embryonic lethality
            due to widespread apoptosis. Heterozygous loss of function
            mutations result in autism spectrum disorder-like behaviors that
            include increased anxiety, repetitive behavior, and altered social
            behavior. [provided by RefSeq, Dec 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: DQ190419.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMN00849374,
                                    SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-83                AC126037.4         92157-92239
            84-1142             AC126037.4         112129-113187
            1143-1520           AC126037.4         114416-114793
            1521-1906           AC126037.4         116987-117372
            1907-2021           AC126037.4         118365-118479
            2022-2204           AC159323.3         221701-221883       c
            2205-2273           AC159323.3         221439-221507       c
            2274-2329           AC159323.3         220816-220871       c
            2330-2447           AC159323.3         220241-220358       c
            2448-2531           AC159323.3         218829-218912       c
            2532-2669           AC159323.3         216685-216822       c
            2670-2791           AC159323.3         215506-215627       c
            2792-3035           AC159323.3         215117-215360       c
            3036-3212           AC159323.3         213800-213976       c
            3213-3356           AC159323.3         212376-212519       c
            3357-3612           AC159323.3         211919-212174       c
            3613-3823           AC159323.3         210445-210655       c
            3824-4019           AC159323.3         210086-210281       c
            4020-4187           AC159323.3         209376-209543       c
            4188-4367           AC159323.3         208672-208851       c
            4368-4478           AC159323.3         208416-208526       c
            4479-4675           AC159323.3         207456-207652       c
            4676-4875           AC159323.3         207027-207226       c
            4876-5032           AC159323.3         206766-206922       c
            5033-5122           AC159323.3         206600-206689       c
            5123-5226           AC159323.3         206019-206122       c
            5227-5356           AC159323.3         204689-204818       c
            5357-5432           AC159323.3         202193-202268       c
            5433-5487           AC159323.3         201438-201492       c
            5488-5695           AC159323.3         201054-201261       c
            5696-5904           AC159323.3         199799-200007       c
            5905-6621           AC159323.3         198993-199709       c
            6622-6773           AC159323.3         198636-198787       c
            6774-7076           AC159323.3         198080-198382       c
            7077-7190           AC159323.3         196707-196820       c
            7191-7370           AC159323.3         196347-196526       c
            7371-7487           AC159323.3         195834-195950       c
            7488-8489           AC159323.3         192354-193355       c
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 26.84 cM"
     gene            1..8489
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /note="chromodomain helicase DNA binding protein 8"
                     /db_xref="GeneID:67772"
                     /db_xref="MGI:MGI:1915022"
     exon            1..83
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            84..1142
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     CDS             300..8048
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /EC_number="3.6.4.12"
                     /note="axis duplication inhibitor; helicase with SNF2
                     domain 1; ATP-dependent helicase CHD8"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 8"
                     /protein_id="NP_963999.2"
                     /db_xref="CCDS:CCDS36919.1"
                     /db_xref="GeneID:67772"
                     /db_xref="MGI:MGI:1915022"
                     /translation="MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLD
                     SLDQMNQDGGGGDVGNSSASDLVPPPEETASTELPKESTAPAPESLTLHDYTTQPTSQ
                     EQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSATGVSPSNTGGQPSQSAPKIV
                     ILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIV
                     SGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLT
                     STPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQP
                     AKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNP
                     GQRLSVPLKMVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASCAPHTAGKTGMEE
                     NRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKS
                     SGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPR
                     EDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPD
                     GETLPSMQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHC
                     EWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDK
                     DNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELRRVNRPQANAW
                     KKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVY
                     NVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGR
                     LIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLE
                     HKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPM
                     MLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLN
                     TMMELRKCCNHPYLINGAEEKILMEFREACHIIPQDFHLQAMVRSAGKLVLIDKLLPK
                     LKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDR
                     FVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITR
                     NSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEE
                     DDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQK
                     WAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRR
                     HDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLL
                     HYRGDENIKSFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKAD
                     WIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEI
                     DIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDK
                     AIAAEHRVLDNFSDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVID
                     GEEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEA
                     AFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDNMQFHWDRFRTFARLDKKTD
                     ESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLRE
                     QVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFS
                     FLAARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTV
                     QVPNLESLTLKLEDEVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSEL
                     DLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTADRSRPKLYDEESLLSLTMSQDGF
                     PNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSNRRSQEVTAG
                     GILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQFTKLRRGM
                     DEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEP
                     NHLDLDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRK
                     QKWQRCKKNNKAELNCLGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVR
                     RTRPDLSKMMALMQGGSTGSLSLHNTFQHSSSNLQSVSSLGHSSTTSASLPFMPFVMG
                     AAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSSPATTTSGTALRLPTLQPE
                     DDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD"
     exon            1143..1520
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            1521..1906
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            1907..2021
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2204
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2273
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            2274..2329
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2447
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            2448..2531
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            2532..2669
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            2670..2791
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            2792..3035
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            3036..3212
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            3213..3356
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            3357..3612
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            3613..3823
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            3824..4019
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            4020..4187
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            4188..4367
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            4368..4478
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            4479..4675
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            4676..4875
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            4876..5032
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            5033..5122
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            5123..5226
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            5227..5356
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            5357..5432
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            5433..5487
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            5488..5695
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            5696..5904
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            5905..6621
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            6622..6773
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            6774..7076
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            7077..7190
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            7191..7370
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            7371..7487
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     exon            7488..8489
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8468..8473
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /note="hexamer: AATAAA"
     polyA_site      8488
                     /gene="Chd8"
                     /gene_synonym="5830451P18Rik; Chd-8; Duplin; HELSNF1;
                     mKIAA1564"
                     /note="major polyA site"
ORIGIN      
        1 gtgctccgcc ggaaggcgcc ttgttctccc ggacggtggg ttgcaactct tgcccaggga
       61 aggaagccgc aggactggct caggagttcc atcttctccc gctgaaggct tagctgcctt
      121 gctccatgaa gttcaggaca atctgacatg cacttgtttc ttgtctcttc atttgaagaa
      181 tagtcttcgt ccactgtgta catttttttt gttaataggg aaggggaggg gagaaagagt
      241 tccccccctc cccttccctc cccggaactt caacaaatac cctttattac tctaccaaga
      301 tggcagaccc catcatggat ctttttgatg acccaaactt atttggcctg gactctctga
      361 ctgatgacag ctttaatcag gtcactcaag accccattga agaagcactt ggactgccaa
      421 gctctctgga ctccttggat cagatgaacc aagatggtgg aggtggtgat gtggggaatt
      481 catcagcaag tgacctggtt cctccaccag aggaaacagc ttccactgaa cttcccaaag
      541 aatccacagc tccagctcca gaatccttaa ccttgcatga ttataccact cagcccacca
      601 gccaggagca gccagcccag cctgtcttgc agacatcaac accaacagca ggactcttgc
      661 aggtctccaa gagccaggag atcttgagcc aagggaatcc tttcatgggt gtctctgcca
      721 caggtgtctc ccccagtaat actggaggac aaccatctca gtcagctcct aagattgtta
      781 ttcttaaagc tccaccaaac tcctccgtca caggcaccca tgtggcacaa attcaggccc
      841 aaggtatcac cagtacagct cagcccctag tggctgggac agccaacggt ggaaaagtca
      901 cttttaccaa agtgctgact ggtacacccc ttcgaccagg tgtatccatt gtctctggta
      961 atacagtgtt ggccacgaag gtccctggga accaggctgc tgttcagcgc attgtccagc
     1021 caagccgacc agtaaaacag ctggtcctcc agccagtaaa gggttcagct cctgctggga
     1081 atcctggggc tgccgggccc ccactgaagc ctgcagttac actgacgtct acacctaccc
     1141 agggtgaatc gaaacgcatc actttggtcc ttcaacagcc acagtccgga ggtccccaag
     1201 gacatcggca tgttgtgtta gggagtctac caggcaagat agtgttacag ggcaaccagc
     1261 tagcagcctt gactcaagcc aagaatgccc aggggcagcc tgccaaagta gtaactattc
     1321 agctgcaggt gcagcagcca cagcagaaaa tccagattgt acctcagcct ccatcatcac
     1381 aaccacagcc acagccgcag ccgccaccct cagcccagcc gttgaccctg tcctctgtgc
     1441 agcaggctca gataatgggg ccaggccaaa acccagggca gagactttct gtaccgctca
     1501 agatggtgct gcagccacag gctggctctt ctcagggagc ctcttctgga ctctctgtgg
     1561 ttaaagttct aagtgccagt gaagtggcag ccctgtcatc cccagcaagc tgtgcccctc
     1621 atactgcagg gaagactggg atggaggaga accgcaggtt ggagcaccag aaaaagcaag
     1681 agaaagcaaa tcggattgtg gcagaggcca ttgctagggc ccgggccagg ggcgagcaaa
     1741 acatacctcg agtcctgaat gaggatgagc tgcctagtgt ccggccagag gaggagggtg
     1801 aaaagaaacg caggaagaag agcagtgggg aaaggctgaa ggaggagaag ccaaagaaga
     1861 gcaagactgc tgctgcctcc aaaacgaagg gcaagagtaa gctaaacacc atcactcctg
     1921 tagtgggcaa gaagagaaag cgtaacacat cgtctgacaa ctctgacgtg gaagttatgc
     1981 ctgcacaatc gccccgggag gacgaagaga gcagcatcca gaagagacgt tcaaaccgcc
     2041 aagttaagcg aaaaaaatat acagaggacc tggatataaa gataacagac gatgaagagg
     2101 aggaggaggt cgatgtaact ggtccaataa aacccgagcc catcctccca gaaccagtgc
     2161 aagagcctga tggcgagact cttccttcca tgcagttctt tgtggagaat cctagtgaag
     2221 aagatgcagc tattgtggac aaagtgcttt ccatgcgagt tgtgaagaaa gagcttcctt
     2281 ctggacaata cactgaggct gaagaattct tcgtcaagta caagaactac tcttatctgc
     2341 actgtgagtg ggcaacgatc tcccagctgg agaaggataa gaggatccac cagaaactaa
     2401 aacggttcaa aacaaaaatg gctcagatga gacacttctt tcatgaagat gaagagcctt
     2461 tcaatccaga ctacgtagag gtggatagga tactggacga gtctcacagt gttgacaagg
     2521 ataatggtga gcctgtaatt tactacctgg taaaatggtg ctctctgccc tatgaggaca
     2581 gtacgtggga gctaaaagag gatgttgatg agggcaagat tcgggaattt aaacggatcc
     2641 agtcaaggca cccagaactg agaagggtga atcgtccaca ggcaaatgcc tggaagaaat
     2701 tggagttatc acatgaatat aaaaacagaa accaattacg ggaatatcag ttagaagggg
     2761 tcaactggct cctctttaat tggtataaca ggcagaactg catcctggct gatgaaatgg
     2821 gattgggcaa aactattcag tcaatcgcct tcttgcagga ggtatataat gtaggcatcc
     2881 atggtccctt tttggtcatt gctccattgt ccaccattac taactgggag cgtgaattca
     2941 atacatggac agagatgaac actattgtgt accatggcag cctggccagc cggcagatga
     3001 tccagcagta tgaaatgtac tgtaaagact cacggggtcg cctcatccct ggtgcatata
     3061 agtttgatgc cttgatcacc acttttgaga tgattttgtc agactgtcct gagcttcgtg
     3121 aaattgaatg gcgttgtgtt atcattgatg aagcccatag gctgaagaac cgtaattgca
     3181 agctacttga tagtctcaag cacatggacc tggagcataa agtgttactc acagggacac
     3241 cgttacaaaa taccgtagag gaactgttca gtctactgca tttcttggaa ccttctcagt
     3301 tcccctcaga atcagaattc cttaaggatt ttggggatct caagaccgag gagcaggttc
     3361 aaaagcttca ggccattcta aagccaatga tgctgaggag actcaaagag gatgttgaaa
     3421 aaaatttggc tcccaaacag gaaactatta ttgaagtaga gctaaccaac atccagaaga
     3481 aatactacag agctatttta gagaagaatt tctccttcct ttccaaaggg gcaggtcata
     3541 ccaatatgcc taatctactg aacacaatga tggagctacg aaagtgctgc aatcacccgt
     3601 acctcatcaa tggtgcagaa gagaaaatcc tgatggaatt tcgggaagct tgccatatta
     3661 tacctcaaga tttccacctg caggctatgg ttcggtcagc tggcaagtta gttctcattg
     3721 acaagttact tccaaagctt aaagctggtg gccataaagt tctgatcttc tcccagatgg
     3781 tacgctgtct agacattctg gaggattatc tgatccagag gaggtactta tatgaacgta
     3841 ttgatgggcg agttagaggc aaccttcgac aagctgctat cgaccgcttt agcaagcctg
     3901 actcagaccg ctttgtcttc ttgctatgta cccgtgctgg tggacttggt attaatctta
     3961 cagctgctga tacctgtatt atctttgatt cagactggaa tccacagaat gacctgcagg
     4021 cccaagcacg ttgtcatcga attggacaga gcaaagctgt gaaggtgtac cgactcatca
     4081 ctcgtaattc ctatgagaga gagatgtttg ataaagctag cctcaagttg ggattggata
     4141 aagctgtgct tcagtccatg agtggtcggg atggcaacat tactggaatt caacagttct
     4201 ccaagaaaga gattgaagat ctcttaagga aaggtgccta tgcggccata atggaggaag
     4261 acgatgaggg ttctaagttt tgtgaggagg acatagatca gatcttactg agacggacca
     4321 caaccatcac tattgagtct gaagggaaag gttctacttt tgccaaggca agctttgttg
     4381 cttctgaaaa taggacagat atttccctgg atgatccaaa cttttggcaa aaatgggcca
     4441 aaaaggcaga cctagacatg gatctgctga atagtaagaa taacttagtg atcgacacac
     4501 ctcgagttcg gaaacagact cgacacttca gcactttgaa agatgatgac ctggttgagt
     4561 tttctgattt ggaaagtgaa gatgatgaac ggccacgttc ccgaagacat gaccggcatc
     4621 acacctatgg gcgcactgac tgcttccggg tagaaaagca tctcctggta tatggttggg
     4681 gacgttggag agatattttg tctcacggac gatttaagcg gcgcatgact gaaagagatg
     4741 ttgagacaat atgccgtgcc atcctcgtgt actgtcttct acactaccgt ggggatgaaa
     4801 acatcaaaag cttcatttgg gacttgatta gccctgctga aaatggcaag acaaaagaat
     4861 tgcagaatca ttcaggtctg tctatccctg tgccccgtgg gcgtaagggg aaaaaagtca
     4921 agtcacaaag tacttttgac attcataagg cagactggat ccggaaatac aaccctgata
     4981 ctctgttcca agatgagagt tataagaagc atttgaaaca tcagtgtaac aaggtactgt
     5041 tgagagtccg gatgctctac tacctgagac aggaggttat tggagaccag gcagagaagg
     5101 tgttaggagg tgccatagcc agtgagattg acatatggtt cccagtggtg gatcagctgg
     5161 aggttcctac aacttggtgg gatagtgagg ctgacaaatc cctgctcatt ggcgttttta
     5221 agcatggcta tgagaaatac aataccatga gggcagaccc tgccttgtgc ttcctagaaa
     5281 aggctggcag gccagatgac aaagccatcg cagcagaaca tagagtgttg gataatttct
     5341 ccgacctggt agaagggatt gactttgata aggattgtga agatcctgaa tataaacccc
     5401 tccagggtcc tccaaaggac ccagatgatg agggtgatcc cttgatgatg atggatgagg
     5461 agatctcagt catcgacgga gaggaagccc aggtaactca acagccaggg catttattct
     5521 ggcctccagg ctccgccctc acagctaggc ttcgccgcct agtaacagcc tatcaacgca
     5581 gctacaagag agaacaaatg aagatggagg ctgcggaacg tggggaccgg agacgccgac
     5641 gatgtgaagc agcctttaag ctcaaagaaa tcgcaaggcg ggaaaaacaa caacggtgga
     5701 caaggcgtga gcaaactgat ttctaccgag tagtatctac ctttggtgtg gagtatgacc
     5761 ctgacaacat gcagtttcac tgggatcgct tccgcacttt tgctcgcctg gacaaaaaaa
     5821 cagatgaaag ccttaccaag tacttccatg gttttgtggc catgtgtcga caagtgtgcc
     5881 gccttccccc agctgctggt gatgaacctc cagaccctaa tctgttcatt gagcccatca
     5941 ctgaagagag agcctcgagg actctctacc gaattgagtt gcttcgacgc ttacgggaac
     6001 aagttttatg ccaccccctt ttagaagatc ggctggcatt gtgtcagcct ccaggtcttg
     6061 aattgcccaa atggtgggaa cctgtccgtc atgatgggga gctcctacga ggagcagccc
     6121 gccatggggt gagccaaaca gactgcaaca tcatgcagga cccagacttc tcttttctgg
     6181 ctgcccgtat gaattatatg cagaaccatc aggcgggagc atcagctgca tccctgtcgc
     6241 gatgctccac tccactgctg caccagcagt gtacctcacg tactgcttca ccatcacccc
     6301 tgcgcccaga tgctcctgtt gaaaagtcac ctgaggaaag tactgttcag gtccccaatc
     6361 tggagagtct gactttaaag ttagaggatg aggttgtggc taggagccga ctcacctcac
     6421 aagactatga agtacgcgta ggctcttcag atacagctcc tctgtcccgg agtgtcccac
     6481 cagtgaaact ggaggacgag gatgattcag actctgagct ggacttgagc aagttgtcac
     6541 catcctcctc ctcttcttca tcctcgtcca gctccagctc cagcactgac gagagtgaag
     6601 acgagaagga agagaagtta actgctgacc ggtcccgccc aaagctctat gatgaagaga
     6661 gccttctgtc ccttactatg tcccaagatg gattcccaaa tgaagatgga gaacaaatga
     6721 cccctgagtt gctgctactg caggaaagac aaagagcctc tgaatggcct aaggatcgtg
     6781 ttctgataaa ccgcattgac ctcgtctgcc aggctgtact ctcagggaaa tggccttcta
     6841 accgccggag ccaggaagtg acagcaggag gaattttggg gccaggcaac catttattag
     6901 acagtccctc tttgacccca ggagaagatg gggactctcc agtccccacg ccacgaagtg
     6961 gcagtgcagc ttccatggcg gaggaagagg catctgcagt caccacagcg gcggcccagt
     7021 ttacgaaact tcggcgaggc atggatgaga aagagtttac agttcagatc aaagatgaag
     7081 aaggattgaa gttaacattc cagaagcata gattgatggc taatggtgta atgggagatg
     7141 gacatccact gtttcataag aagaagggga acaggaaaaa gctagtagag ctggaggtgg
     7201 agtgcatgga agagcctaat caccttgatc tggacctgga gacccggatc cctgtcatca
     7261 ataaggtgga tggtactttg ctggtgggtg atgaggcccc tcgccgggcg gagctggaga
     7321 tgtggttaca gggtcatcca gagtttgctg ttgatccccg atttctagcg tatatggagg
     7381 aacgtagaaa acagaagtgg cagagatgta aaaaaaataa taaggcagaa ttgaactgtt
     7441 tgggaatgga accagtacag ccagcgaact ctagaaatgg gaaaaagggt cactatgctg
     7501 aaactgcgtt caaccgggtt ttgccagggc ctgttgcacc agagaacagc aagaaacggg
     7561 tccgtaggac aagaccagac ctttctaaga tgatggccct gatgcagggt ggaagcactg
     7621 ggtccctgtc tctgcataac acctttcaac acagcagtag taacctacag tctgtgtcgt
     7681 ctctgggtca cagcagtacc acttctgcat ctctgccttt tatgccgttt gtgatgggtg
     7741 cagcagcacc ccctcatgta gactccagca ccatgcttca tcatcaccac caccatcccc
     7801 acccccacca tcaccaccat caccatccag gcttgagaac cactggctac ccttcttcac
     7861 cagctactac cacctctggt actgccttgc ggttaccaac actgcaacct gaggacgatg
     7921 atgaagagga ggatgaagaa gatgatgatt tatctcaggg ctatgacagc tcagaacggg
     7981 acttctcact cattgatgac cctatgatgc cagccaactc agactccagt gaagatgctg
     8041 acgactgaag ccccatatag acccgtgatg ggtggctgct ggaatccctt actctggccc
     8101 ttggactttg gaaatgtggg aggggcaggg aacaatatgg ggaaacccaa gacttcagga
     8161 ggtgaaaaga aaaagaaaaa aacaaaaaac aaaaaaaaac aaaaaacttg tacccaattg
     8221 ctcccaattt tgagaggatt gggtgggcag gggaactcct aaaaaaaaaa aataataaat
     8281 gaccacttcc tcgtttctgg ggaagcaaag gagcttagag cagctggtgt gctcacccct
     8341 ccccagacgc ctccattaac cacagactat gtagcgctgg ccctagcctc tggcagagcc
     8401 tgttcctggc caaactgtgg ctacagctgg agggtcagga actgttacct tccttcccct
     8461 tggcattaat aaatttaagt taatccttt
//
