LOCUS       NM_173368              10579 bp    mRNA    linear   ROD 18-OCT-2024
DEFINITION  Mus musculus chromodomain helicase DNA binding protein 6 (Chd6),
            transcript variant 1, mRNA.
ACCESSION   NM_173368 XM_485094
VERSION     NM_173368.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10579)
  AUTHORS   Elsen,G.E., Bedogni,F., Hodge,R.D., Bammler,T.K., MacDonald,J.W.,
            Lindtner,S., Rubenstein,J.L.R. and Hevner,R.F.
  TITLE     The Epigenetic Factor Landscape of Developing Neocortex Is
            Regulated by Transcription Factors Pax6--> Tbr2--> Tbr1
  JOURNAL   Front Neurosci 12, 571 (2018)
   PUBMED   30186101
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10579)
  AUTHORS   Stanley,F.K., Moore,S. and Goodarzi,A.A.
  TITLE     CHD chromatin remodelling enzymes and the DNA damage response
  JOURNAL   Mutat Res 750 (1-2), 31-44 (2013)
   PUBMED   23954449
  REMARK    Review article
REFERENCE   5  (bases 1 to 10579)
  AUTHORS   Alfonso,R., Rodriguez,A., Rodriguez,P., Lutz,T. and Nieto,A.
  TITLE     CHD6, a cellular repressor of influenza virus replication, is
            degraded in human alveolar epithelial cells and mice lungs during
            infection
  JOURNAL   J Virol 87 (8), 4534-4544 (2013)
   PUBMED   23408615
REFERENCE   6  (bases 1 to 10579)
  AUTHORS   Lathrop,M.J., Chakrabarti,L., Eng,J., Rhodes,C.H., Lutz,T.,
            Nieto,A., Liggitt,H.D., Warner,S., Fields,J., Stoger,R. and
            Fiering,S.
  TITLE     Deletion of the Chd6 exon 12 affects motor coordination
  JOURNAL   Mamm Genome 21 (3-4), 130-142 (2010)
   PUBMED   20111866
  REMARK    GeneRIF: The genomic location, expression pattern, and ataxic
            phenotype of Chd6 Exon 12 -/- mice indicate that mutations within
            CHD6 may be responsible for ataxias.
REFERENCE   7  (bases 1 to 10579)
  AUTHORS   Lutz,T., Stoger,R. and Nieto,A.
  TITLE     CHD6 is a DNA-dependent ATPase and localizes at nuclear sites of
            mRNA synthesis
  JOURNAL   FEBS Lett 580 (25), 5851-5857 (2006)
   PUBMED   17027977
  REMARK    GeneRIF: CHD6 colocalizes with both hypo- and hyper-phosphorlylated
            forms of RNA polymerase II. CHD6 was found to be present at sites
            of mRNA synthesis and to be part of a high molecular weight
            complex.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ075254.1, AK080892.1,
            AK040495.1, AK142177.1, AK018129.1, CN664107.1, CV559963.1,
            BU708927.1, CO433513.1, CN526286.1, CN664285.1 and AL590430.8.
            
            [WARNING] On Oct 22, 2024 this sequence was replaced by
            NM_173368.4.
            
            On Nov 21, 2014 this sequence version replaced NM_173368.2.
            
            Summary: This gene encodes a member of the
            chromodomain/helicase/DNA-binding domain family of chromatin
            remodeling enzymes. This protein has been found to be specifically
            involved in transcription initiation and elongation. Homozygous
            knockout mice exhibit impaired motor coordination. A pseudogene has
            been identified on chromosome 8. Alternative splicing of this gene
            results in multiple transcript variants. [provided by RefSeq, Nov
            2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. This variant encodes the protein.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                CJ075254.1         5-15
            12-64               AK080892.1         2-54
            65-917              AK040495.1         2-854
            918-2387            AK142177.1         852-2321
            2388-2388           AK018129.1         2321-2321
            2389-4034           AK142177.1         2323-3968
            4035-4035           CN664107.1         586-586
            4036-5323           AK142177.1         3970-5257
            5324-5867           CV559963.1         57-600
            5868-6339           BU708927.1         139-610
            6340-6917           CO433513.1         121-698
            6918-6918           CN526286.1         726-726
            6919-6972           CO433513.1         699-752
            6973-7312           CN664285.1         171-510
            7313-7369           AL590430.8         20096-20152         c
            7370-10579          AL590430.8         10991-14200         c
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 80.98 cM"
     gene            1..10579
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /note="chromodomain helicase DNA binding protein 6"
                     /db_xref="GeneID:71389"
                     /db_xref="MGI:MGI:1918639"
     exon            1..98
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            99..154
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     CDS             122..8257
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /EC_number="3.6.4.12"
                     /note="ATP-dependent helicase CHD6"
                     /codon_start=1
                     /product="chromodomain-helicase-DNA-binding protein 6"
                     /protein_id="NP_775544.2"
                     /db_xref="CCDS:CCDS17000.1"
                     /db_xref="GeneID:71389"
                     /db_xref="MGI:MGI:1918639"
                     /translation="MKMKIQKKEKQLSKLRALNHSPMSDASVNFDYKSPSPFDCSPDQ
                     GENIEEAANHCLPHKNLYTTEEEADTLFSRKLTSHNGMEDSGGRGTGVKKKRKKKEPG
                     EQEGTKGSKDREPKPKRKREPKEPKEPRRAKEPKRAKEPKETKQKDGVKKPRKHREAS
                     GTKEGKEKRSCTDYGSRTKSKKASREQGPTPVERKKKGKRKNETTVESLELDHSLPNP
                     SLQSPEEPSESADSQKRRSGRQVKRRKYNEDLDFKVVDDDGETIAVLGAGRTSALSAS
                     TLAWQAEEPPEDDANIIEKILASKTVQEVHPGEPPFDLELFYVKYRNFSYLHCKWATM
                     EELEKDPRIAQKIKRFRNKQAQMKHIFTEPDEDLFNPDYIEIDRILEVAHTKDAETGE
                     EVTHYLVKWCSLPYEESTWELEEDVDPAKVKEFESLQILPEVKHVERPASDAWQKLET
                     SREYRNSNRLREYQLEGMNWLLFNWYNRKNCILADEMGLGKTIQSIAFLSEIFVRGIH
                     GPFLIIAPLSTITNWEREFRTWTEMNAIVYHGSQISRQMIQQYEMVYRDAQGNPLSGV
                     FKFHVVITTFEMILADCPELKKIHWSCVIIDEAHRLKNRNCKLLEGLKLMALEHKVLL
                     TGTPLQNSVEELFSLLNFLEPSQFPSETAFLEEFGDLKTEEQVKKLQSILKPMMLRRL
                     KDDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLTKGANQHNMPNLINTMMEL
                     RKCCNHPYLINGAEEKILEDFRKAHSSEASDFQLQAMIQAAGKLVLIDKLLPKLIAGG
                     HKVLIFSQMVRCLDILEDYLIQRRYTYERIDGRVRGNLRQAAIDRFCKPDSDRFVFLL
                     CTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYER
                     EMFDKASLKLGLDKAILQDINRKGSTNGVQQLSKMEVEDLLRKGAYGALMDEEDEGSK
                     FCEEDIDQILQRRTHTITIQSEGKGSTFAKASFVASGNRTDISLDDPNFWQKWAKIAE
                     LDTEANNEKESLVIDRPRVRKQTKHYNSFEEDELMEFSELDSDSDERPTRSRRLSDRA
                     RRYLRAECFRVEKNLLIFGWGRWKDILTHGRFKWPLNEKDMEMICRALLVYCVKHYKG
                     DEKIKSFIWELITPSKDGQVQTLQNHSGLSAPVPRGRKGKKTKNQLLLPELKNADWLA
                     TCNPEVVLHDDGYKKHLKQHCNKVLLRVRMLYYLKAEILGEAADKAFEGTPARELDVL
                     LPDIDYVEIPVDWWDAEADKSLLIGVFKHGYERYNAMRADPALCFLEKVGMPDEKSLS
                     AEQGVTDGTSDIPERGNIDKEDSAEDKLDGLQKQTASPSDGSDGIFGEKKDDSQAAQD
                     GSDPDKSPWPVSSALTARLRRLVTIYQRCNRKELCRPEILGPGNQGYWVQEEVFRRTS
                     EMDLINKEAQKRWTRREQADFYRTVSSFGVVYDQEKKAFDWTQFRIISRLDKKSDESL
                     EHYFYSFVAMCRNVCRLPAWKDDGPPDASIYVEPITEERAAKTLYRIELLRKVREQVL
                     MCPQLHERLQLCRPSLYLPVWWECGKHDRDLLIGTAKHGLNRTDYYIMNDPQLSFLDA
                     YRNYAQHKRTDTQAPGSLCCLYQSNSKLYESLTYTPVSRTSESLESEPENLMRMESRD
                     DHLCLPEGGLPDITCENFVSKVQEVISLDHDENLLPESLENMIYGKTGLSQEPHSFQE
                     APTTNTQSRKNTITISASRNESCQPPGIEAEITSASSLMSSLEAGVAKMNIKNGKHLL
                     VSISKEGEPCCSETGRRPETIGHREAKCLVSPTLDTGHESGFVDLCSLSVYDPKRNFS
                     SDQQLIDLLENKSLESKLILNQSDEEEEENEDETLAIVASATEKPEVLDFPKPTVNIP
                     RGKNLSFHQDEAKKGRLEVVSKTAGPQRVFPPPANQCHCKHIERWAHGLGSEDSEVEK
                     PKAYQPDLYRSKANNSTVEGETAVIPTEPFKLKHELLKEPWKESSEGGKSFSMYAPEG
                     SEPKPEDMDFENKDDYEKDGTCLSQDYPGKYSEEESKSSASGIAGDLGEEAQEVRAPT
                     IAQLLQEKTLYSFSEWPKDRVIINRLDNICHVVLKGKWPCSHQYEPSGALPTPVLSSS
                     AGSRSSLSEPEATEHGFSNGAALAAQIQKESFLAPVFTKDEQKHRRPYEFEVERDAKA
                     RSLEEYSATHGRPPIVLNGWHGESAIDLSCSSEGSPGATSPFPVSASTPKIGAISSLQ
                     GALGMDLSGILQAGLIHPVTGQIVNGSLRRDDAAMRRRRGRRKHIEGGMDLIFLKEQT
                     LQAGILEVHEDAGQTTLSTTHPEVPGATSSAPEPTAAASSQAEKAVPSKSLLDWLRQQ
                     ADYSLDVPGFGTSFSDKPKQRRPRCKEPGKLDISSLGGEERVPAVPKEPGLRGFLPES
                     KFNHTLAEPVLRDAGPRRRGRRPRNELLKAPAIVADSPSGMGPLFMNGLIAGMDLVGL
                     QNVRNIPGIPLTGLVGFPAGFATMPTGEEVKNTLSMLPMMLPGMAAVPQMFGVGGLLN
                     TPMATTCTTTASASLASTKSGTSATEKSTEDKLSGHDVNTDALVDDKPGPSPFSDQSE
                     PTITTSSPVAFNPFLIPGVSPGLIYPSMFLSPGMGMALPAMQQARHSEMVGLETQKRK
                     KKKTKGDSPTQEPASVCEKEPGSDQNCTESSATVSPEREHVAQAREEGLKDSNEDTN"
     exon            155..672
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            673..820
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            821..970
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1033
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1092
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1093..1210
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1297
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1432
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1433..1554
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1798
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..1975
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            1976..2119
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2375
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2586
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2782
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2783..2947
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..3127
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3128..3238
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3239..3441
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3442..3641
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3642..3801
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3802..3891
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3892..3995
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            3996..4125
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4126..4186
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4187..4247
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4248..4464
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4465..4673
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            4674..6245
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            6246..6397
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            6398..6604
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            6605..7057
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            7058..7249
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            7250..7369
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     exon            7370..10579
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9822..9827
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /note="hexamer: AATAAA"
     polyA_site      9844
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /note="major polyA site"
     regulatory      10090..10095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /note="hexamer: AATACA"
     polyA_site      10112
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /note="major polyA site"
     polyA_site      10475
                     /gene="Chd6"
                     /gene_synonym="5430439G14Rik; 6330406J24Rik; CHD-6"
                     /note="major polyA site"
ORIGIN      
        1 gggcccaagc tgggttaggg tgtccttgca gggtgtctga gctaaacttc accaaataat
       61 agctgtttgt attttggctg ctgcaggagc cattttagaa ataaatacct tcccttaatt
      121 gatgaaaatg aaaatacaga agaaagagaa gcagttgtcc aagttaagag ctttgaatca
      181 ctccccaatg tcggatgcct ctgttaactt tgactacaaa tctccgtcac cattcgattg
      241 cagtcctgat cagggagaga acattgaaga agctgccaat cattgtctgc cccataagaa
      301 cctctatact actgaagagg aagctgacac ccttttttct aggaaattga catcccataa
      361 tgggatggag gatagtggag gcagaggtac tggagtaaag aagaaacgga agaaaaagga
      421 gccaggggag caagagggga caaagggaag caaggacaga gagcctaagc caaagaggaa
      481 gcgagaacct aaagagccaa aagaacccag aagggccaag gagccgaaga gagccaagga
      541 gcccaaggag acaaagcaga aagacggtgt caagaagccc cggaagcacc gggaggcctc
      601 cggtaccaag gaaggtaaag agaagagaag ctgcactgat tatggatcca ggacaaagtc
      661 caagaaggcc agcagggagc aaggaccaac cccagtggag agaaagaaga aaggaaaaag
      721 gaaaaacgaa accaccgtgg agagtttaga gctggaccac agtcttccaa acccatctct
      781 gcagagccct gaggagccca gtgagtctgc agacagccag aaacggcgct caggacggca
      841 agtaaagcgc agaaaataca atgaggacct ggacttcaaa gtggtggatg acgatgggga
      901 aacaattgct gtcctgggag ctggccgcac atctgcactc tcggcttcca ctctggcctg
      961 gcaggctgag gagcctccag aagatgatgc aaacataatt gagaagatcc tggcatccaa
     1021 gactgtccag gaggttcacc caggagaacc tccattcgac ctagagcttt tctacgttaa
     1081 gtatagaaat ttttcctatt tacattgtaa atgggccaca atggaagaac tggaaaagga
     1141 tccccgcatc gcacagaaga tcaagcgatt taggaacaaa caggcccaga tgaagcacat
     1201 tttcactgag cctgatgaag acttgttcaa tccagattat atagaaattg atcgtatctt
     1261 agaagtggcc cacaccaagg atgcagaaac aggggaggaa gtaacacatt acctggtaaa
     1321 atggtgctcc ctgccatatg aggaaagcac ctgggagcta gaagaagatg tggatcctgc
     1381 aaaagtgaag gaatttgaat ctctacaaat tctccctgaa gttaaacatg tggaacgacc
     1441 tgcctctgat gcgtggcaga aactggagac atcccgtgag tataggaaca gtaaccggct
     1501 gcgagagtac caactcgagg ggatgaactg gcttcttttc aactggtaca acagaaaaaa
     1561 ctgtatattg gctgatgaaa tgggcctagg gaaaaccatc cagtccatcg cattcctttc
     1621 agaaatcttt gtcagaggaa tccacggccc ctttctcatc attgccccac tttccaccat
     1681 cactaactgg gagcgagaat tccggacatg gacggagatg aatgctattg tgtaccacgg
     1741 cagccagatc agcaggcaga tgatccagca gtacgaaatg gtatacagag atgcacaggg
     1801 aaaccccctt tcaggagtct tcaagttcca cgtcgtcatc acaacgtttg agatgatcct
     1861 ggcagattgc ccagagctga agaaaattca ctggagctgt gtgataattg atgaggccca
     1921 cagactgaag aataggaact gcaaacttct ggaagggtta aagctcatgg ccctggaaca
     1981 caaagtgctg ctcacaggaa cacccttgca gaactctgtg gaagagctct tcagtttgct
     2041 taactttctg gagccctcgc agttcccttc agagactgcc ttcttagagg agtttggtga
     2101 tctcaaaaca gaagaacagg taaagaaatt gcagtctatc ctaaaaccaa tgatgcttcg
     2161 gcgcctgaaa gatgatgtgg agaagaacct tgctcctaag caagagacca tcattgaagt
     2221 agaactgacc aatatccaga aaaagtacta tcgtgctatc ctagagaaaa acttttcctt
     2281 cctgaccaaa ggtgcaaatc aacacaacat gcccaatctt atcaatacta tgatggagct
     2341 cagaaaatgc tgtaaccacc cctacctgat caatggggcc gaggaaaaga ttctggaaga
     2401 tttcagaaaa gcgcacagct ccgaagcctc tgacttccag ctgcaggcca tgatccaggc
     2461 ggccgggaag ctggtgctga ttgacaagct tctccccaag ctcattgccg ggggccacaa
     2521 agtgctcatc ttctcgcaga tggtgcgctg cctcgacatc ctggaagatt atctcatcca
     2581 gaggagatac acctatgagc gaattgatgg gcgagtacgg ggaaacctgc gccaggcagc
     2641 catcgaccgg ttctgtaagc cagactcaga ccgctttgtc tttcttctgt gcaccagagc
     2701 gggaggcctg ggaatcaatc tcacagctgc tgatacctgc atcatatttg attctgactg
     2761 gaacccacaa aatgacttgc aggctcaggc ccgatgtcat cgtataggcc agagcaaagc
     2821 cgtgaaggta tatcgcctaa tcactcgaaa ctcctatgag cgggaaatgt ttgacaaggc
     2881 cagcctgaag ctgggcctgg acaaggctat tcttcaggac atcaaccgaa agggcagcac
     2941 caatggagta cagcagctct caaaaatgga agtggaggat ttgctccgga aaggtgctta
     3001 tggggcttta atggacgagg aagacgaagg ctccaagttc tgtgaggaag atatagacca
     3061 gatcctgcag agaagaaccc acaccatcac catccagtca gaaggcaagg ggtccacttt
     3121 tgccaaggct agtttcgtgg cttcaggaaa cagaacagat atttccttgg atgaccctaa
     3181 tttttggcag aaatgggcta aaatagctga attagacact gaagcaaata atgaaaagga
     3241 aagcttagtg attgaccggc cgcgtgtgag gaagcagacc aagcactaca actcttttga
     3301 ggaggacgag ctcatggagt tctcagagtt agacagcgac tcagacgaaa ggcccacacg
     3361 gtccagacgc ctcagcgaca gagcccgacg ctacctccgc gctgagtgct tccgagtgga
     3421 gaagaacctg ctcatctttg gctggggccg gtggaaggac atcctgactc acgggcgatt
     3481 caagtggccc ctgaatgaga aggatatgga gatgatttgc agggctctgc tggtgtactg
     3541 tgtcaagcat tacaaagggg atgagaaaat caaaagtttt atttgggaac taatcacacc
     3601 ttccaaagac gggcaagttc agaccctcca gaaccactca ggcttgtctg ccccagtccc
     3661 cagagggagg aaaggaaaaa agacaaaaaa ccagctcttg ctcccagaac tgaagaatgc
     3721 agactggctg gccacctgca atcctgaggt ggtcttacat gatgatggat acaagaaaca
     3781 cctcaaacag cattgcaata aggtgctttt gagagtccgg atgctgtact acctaaaagc
     3841 tgaaatactg ggagaagcag ctgataaagc attcgaggga actcctgcca gagagctgga
     3901 tgtgcttctg cctgacattg actatgtgga gatcccagtg gattggtggg acgctgaagc
     3961 tgataagtcc cttctcattg gcgtgttcaa gcacgggtat gagaggtaca atgccatgcg
     4021 ggctgaccca gcgctctgct tcctagagaa agttggcatg cctgatgaga agtccctttc
     4081 tgcagagcag ggagttacag atggaacttc ggacattcct gaaagaggca acatagataa
     4141 agaagacagt gctgaggaca agttggatgg tctccagaag caaacggcga gtcccagtga
     4201 tggaagtgat ggcatttttg gtgaaaagaa ggatgacagc caggcagccc aggatggctc
     4261 cgacccagac aaatcaccct ggccagtttc ctctgccctc actgctcgac tcaggcgtct
     4321 ggtcactatt taccagcgct gcaaccgcaa ggagctatgc agacctgaaa tcttaggccc
     4381 aggtaaccaa ggatactggg ttcaggagga agtgttcagg agaacttcag aaatggacct
     4441 catcaacaag gaagcccaaa aaaggtggac tagaagagag caagctgact tctataggac
     4501 agtgtcttcc tttggtgttg tgtatgacca agaaaagaaa gcctttgact ggacacagtt
     4561 ccgcatcatt tctcgtttgg acaagaagtc agatgagagc ctggagcact atttttatag
     4621 ctttgtagcc atgtgtcgga atgtgtgtcg tctccccgca tggaaagatg atggtccccc
     4681 tgatgccagc atctatgttg aacccatcac tgaggagcgg gcagcaaaga ctctataccg
     4741 tattgaactg ctgaggaaag tccgtgagca ggtgctcatg tgtcctcaac tacatgagcg
     4801 cctccagctt tgcaggccca gcctctacct cccagtctgg tgggagtgtg ggaaacatga
     4861 tcgagacctg ctcattggca ccgccaaaca tgggctcaac cgcactgact attacatcat
     4921 gaatgacccc cagctatcct tcctagatgc ctatagaaac tatgcccaac acaaaagaac
     4981 tgacacccag gcacctggaa gtctctgttg cctttaccag agcaattcta aactatatga
     5041 gtctcttact tacaccccag tgagtaggac ttcagagtct cttgaaagtg agccagagaa
     5101 tctgatgaga atggaaagca gggatgatca tctctgcctg cctgagggcg gcttacctga
     5161 cataacgtgt gaaaactttg tttccaaagt tcaggaggtc atttctcttg accatgatga
     5221 aaacttgctg cctgagtctt tggagaacat gatatatggt aagacaggac tcagccaaga
     5281 gccgcattct tttcaggagg ccccaactac caacacacag tccaggaaaa atactatcac
     5341 catctcagcc agcagaaatg agagctgcca gcctcctggt attgaggcag aaattacttc
     5401 agcttcctct ctcatgagca gcttagaagc aggagtagct aaaatgaata ttaaaaatgg
     5461 aaaacatttg ctggtatcta tctcaaaaga aggggagccc tgctgcagtg agacaggacg
     5521 gagacctgaa accataggcc accgagaggc caaatgctta gtgtccccta ccttagatac
     5581 aggacatgaa agtgggtttg tagatctatg tagtcttagt gtctatgacc ccaaaagaaa
     5641 cttctcctca gatcagcaat taattgactt attagaaaac aagagtttag aaagtaagtt
     5701 gattttaaat cagagtgatg aggaagagga agaaaatgag gacgaaacct tagccatagt
     5761 agcaagcgcc acagaaaagc cagaggtctt ggatttcccc aagcccactg taaacatccc
     5821 aagggggaag aacctcagct tccaccaaga tgaagccaag aaaggaaggc tggaggtggt
     5881 gagcaagact gctggaccac agagggtctt tcctccccca gccaatcagt gccactgtaa
     5941 acacatcgaa aggtgggcac atggcctggg gagcgaggac tctgaagtag agaaacccaa
     6001 ggcttatcag ccagacctgt acagaagcaa agccaataat tccacagtgg aaggtgagac
     6061 tgctgttatt ccaacagagc catttaaact gaagcatgag cttttaaaag aaccatggaa
     6121 agaaagttca gaggggggaa agagtttctc catgtatgct cctgagggaa gtgagcccaa
     6181 accagaagac atggattttg agaataaaga tgattatgag aaagatggaa cctgcctcag
     6241 tcaagactac ccaggcaaat actctgagga agagagtaag agttcggcat caggcattgc
     6301 aggagacctt ggggaagagg cacaggaagt gcgggctccc accattgctc agctactgca
     6361 ggagaaaacc ctctattcct tctcagaatg gccaaaggat cgtgtgataa ttaaccgcct
     6421 agataatatt tgccatgtgg tgttaaaggg gaagtggccc tgcagccacc agtatgagcc
     6481 ctcaggtgca ctgcccacac cagtgttatc cagcagtgca ggttctcgaa gcagcctctc
     6541 agagccagaa gcaacagagc acggcttcag caatggtgca gcactggcag cccagatcca
     6601 gaaggagagc ttcttagccc cagtattcac aaaagatgaa caaaagcaca gacgtcccta
     6661 tgagtttgag gtggaaaggg atgccaaagc tcggagccta gaagaatact ctgccaccca
     6721 tgggcgtccg cccattgtcc tcaatggctg gcatggggag tcagctatag acctctcctg
     6781 ctcctcagag gggtctccag gagccacatc tcctttccca gtgagtgcca gcacccctaa
     6841 gattggtgct atcagttcac ttcaaggagc tctcggtatg gacttgtctg gaattctgca
     6901 agctggtcta atccacccag tgactgggca gattgtcaac ggaagcctca ggagggatga
     6961 tgctgccatg agaaggcgga gaggaaggcg aaaacatatt gagggaggga tggacctcat
     7021 cttcctgaag gagcaaacac ttcaggctgg aatcttggaa gtccatgaag atgcagggca
     7081 gactaccctg agcaccacac accctgaagt gccaggagct acttcctcag ctcctgagcc
     7141 aacagcagca gccagcagcc aggctgagaa agctgttccc agcaagagcc tacttgactg
     7201 gctgaggcag caggctgact actctttgga tgttcctggc tttgggacaa gtttttcaga
     7261 caaacccaag cagaggagac cacgctgcaa agaacctgga aaattagaca tcagctctct
     7321 aggtggagaa gagagggttc ctgctgtccc caaggagcca ggactgaggg ggtttctccc
     7381 agaaagcaaa ttcaatcaca ccctagctga gcctgtgctt cgagatgcgg gtccccgcag
     7441 gagggggagg cggcctcgaa atgaactcct aaaagctcca gccattgtag cggactctcc
     7501 ttctggaatg ggaccactgt tcatgaatgg gctgattgcg ggaatggacc tggtaggatt
     7561 gcagaatgtg agaaatattc caggcattcc tctcactggg ctggtgggct tcccagctgg
     7621 ttttgccacc atgcccacgg gtgaagaggt taagaacacc ctgagcatgc tgcccatgat
     7681 gctgccaggc atggccgcgg tgccccagat gtttggagtt ggcggactcc tcaacacacc
     7741 catggcaacc acctgcacga ccactgcttc agcgtctctt gcaagcacaa aaagtggtac
     7801 ctcagccact gaaaaatcta cagaggacaa gctgagtggc catgatgtaa acacagacgc
     7861 tctggtagac gacaagcctg gccctagtcc gttttctgat cagtcagaac ccacaataac
     7921 tactagtagt cctgtggctt tcaacccatt tctcatccca ggagtgtctc ctggactcat
     7981 ctacccttcc atgtttcttt cccctggcat gggcatggct ctgccagcca tgcagcaggc
     8041 cagacactca gagatggtcg gtcttgagac ccagaagagg aaaaagaaga aaactaaggg
     8101 ggacagcccc acccaagagc ctgcgtctgt gtgtgaaaag gagccaggca gtgatcagaa
     8161 ctgcaccgag tccagtgcca ctgtgtcccc agagagagaa cacgtggcac aggccaggga
     8221 ggaggggctc aaagactcca atgaggatac caattagaac tttttcattt aagaaatgat
     8281 tgtggcttgt aagtttctta tcccataaag gtttgtcact tccctcactt caccttcata
     8341 agaacctgtg ttttcatcag taatattacc tacctaattt cctgtctgag aactctggtg
     8401 acatgttaat agttgcaggg tcttgccact ttgttaggta agtgtcacct gcctagtcta
     8461 ggcggcacag aatccttcct tttatccagt tcattccagc aatcacagtt gatacagtgc
     8521 ttggtgacat accaccttcc ccttctcttt caagtcccac tcatctgagg aagaaaatgg
     8581 cagaagaaag ctgtgtaggg attcactgtg gggccaaaga gcaaagatga acgtaagtgc
     8641 aagctcgttc ttttgtgagc aactgaataa aaagatcata ttgaggaaac caaaaggcac
     8701 atgataggaa gtgccggtgc gtacttttgt agttcaggtg tcctgttgtc ttggttactg
     8761 agtagcgtag tctacagcat ataaactaat ggttcaaaat gcatatactg atggcttcta
     8821 aaaacaggaa ccttccagat agtaatacag tttccccctc aaggttttcc tcctcttcag
     8881 caagaagaaa tactggccag ctaggcagtt tccctatcgt ccctgctgag tctgagacac
     8941 ttcagcacct tgagttactg cagcaactgc gagggcgctg ctcagggtcc acagatgctg
     9001 ctggcattta tcccaggaca actgctgcca cccgtttcca cttttaagaa acttgctgga
     9061 tttttgtgtt ctttcaccca gagcaaggct ttttggagcc tagtaaggta atcgctcagc
     9121 acttgctaga ttctgtcatt agcgtagcgt gtcaaaacac gcacaccacc ttttacattg
     9181 caactgtgaa gttttgtccc tcccattttc tcactactca aacggtatct tagtattggg
     9241 cccaaattcc aaattgtcag tcaggcaagg ttctgttaag tcagcagcat acactctcca
     9301 gctccgtgtt gaagaattga gcagggagga gcccactcgg tggcgcttca gttcccgttt
     9361 tctttcccct cacccttaca tcatgttcct cctgagggtc tcctccaagg acagcccctg
     9421 ctgagacttg aaccagccaa acctagttct ctggagcaca ggctcagggg ctagacaggt
     9481 ggcgggtgtc ccgagtacca ggttcacttc ggtgttacaa ttaactgtgt tttttttact
     9541 gtttctgtta cagttttgct aacttatcag aaggtcggaa agtttggctg tttcagtttg
     9601 cattatttat ttatgatgat ttttgtcatt gtcttttata cgtttgggtt ataaattatg
     9661 taaatgttaa aatgagcatc tcaaagaagt ctgttaaatt gtgaccggaa aaaaaaaaac
     9721 aatcaggtgt attttcaaaa cagagagtcc cagttttatg aatcatagat ataaactaga
     9781 aattctataa cgatctataa gaaagaagaa tccaagaact gaataaatgc tatttaataa
     9841 cctccttgta gtgtagaagg tctgtgatgc tggtaacaga tttcttagaa atgctgctca
     9901 ctgttgaact aacatcctgt tcagttctgc tccggcggaa gcagaaaggt ttctcggctg
     9961 ttaaatccta aagatcaata taatttattt atgtaagaga aacatgcaat caatatattt
    10021 ttgaaccttt taagtactaa ttttcttttt atcaagtaga aaaaaatgta tttgccctaa
    10081 atccttaaaa atacaaatgc tataaaacct cctgtatttt gcaagcctta ctgcaatgag
    10141 cattgtagac acccagcagg gtctgctctt cctgtcgtct cccgtgttgt ctcatgtgga
    10201 gagctcctct cccagcttcc acctaaactt ccaagtaggt cacagggtcc gctgcggacc
    10261 caacttaacc accagcatgc gcaatccctt gttatatctt aaccgaacct gtatgttaac
    10321 tctcgggtgt tcaggcttct gttgactgct gttgtgatac tgtttgcaaa atgaaattga
    10381 cactgtggat tatttgctct gtaaggcata agtgcttctt atgattattt taacgttccg
    10441 agagcaaaag ccaaatttaa actctcttca gcaaacttga gcctcttcat ctaattccat
    10501 gtcactcata gccataactt ctcttggtca tcttcaccca tgtttcacat aataaattgt
    10561 ctgtggtctt gatcctgga
//
